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152 results on '"Boccaccini, Aldo R."'

3. Contributors

Author

Abraham, Gustavo A., primary, Ahlfeld, Tilman, additional, Alini, Mauro, additional, Allen, Josephine B., additional, Ambrosio, Luigi, additional, Arango-Ospina, Marcela, additional, Armiento, Angela R., additional, Baleizão, Carlos, additional, Beckmann, Matthias W., additional, Beier, Justus P., additional, Best, Serena M., additional, Bleisinger, Nathalie, additional, Boccaccini, Aldo R., additional, Cai, Aijia, additional, Caracciolo, Pablo C., additional, D’Amora, Ugo, additional, Dittrich, Ralf, additional, Epple, Matthias, additional, Farinha, José Paulo S., additional, Fehnel, Alexandra, additional, Gelinsky, Michael, additional, Hahn, Artur, additional, Huang, Jie, additional, James, Bryan D., additional, Kannan, Rahasudha, additional, Kingham, Paul J., additional, Kuna, Vijay Kumar, additional, Kurz, Felix T., additional, Kuth, Sonja, additional, Lee, Sangwon, additional, Liu, Jessica Z., additional, Liverani, Liliana, additional, Lu, Helen H., additional, Ludtka, Christopher M., additional, Ma, Peter X., additional, Mano, João F., additional, Mansbridge, Jonathan, additional, Morgante, Debora, additional, Mouriño, Viviana, additional, Nazhat, Showan N., additional, Oliveira, J. Miguel, additional, Park, Hyeree, additional, Parmentier, Laurens, additional, Pina, Sandra, additional, Popov Pereira da Cunha, Matthäus D., additional, Raucci, Maria G., additional, Reis, Rui L., additional, Rivero, Guadalupe, additional, Ronca, Alfredo, additional, Rosenzweig, Derek H., additional, Ruhl, Tim, additional, Ruther, Florian, additional, Schäfer, Benedikt, additional, Sokolova, Viktoriya, additional, Southgate, Jennifer, additional, Tavares, Márcia T., additional, Ursino, Heather L., additional, Vernengo, Andrea J., additional, Van Vlierberghe, Sandra, additional, Vogt, Lena, additional, and Wei, Guobao, additional

Published
2022
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4. Preface

Author

Boccaccini, Aldo R., primary, Ma, Peter X., additional, and Liverani, Liliana, additional

Published
2022
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6. Investigation of catalytic activation of peroxydisulfate on cu-doped mesoporous silica-based particles (Cu-BMS) for efficient degradation of methylene blue

Author

Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Universidad de Sevilla. TEP123: Metalurgia e Ingeniería de los Materiales, European Union's Horizon 2020 Research and Innovation Programme under grant agreement No 739566, Sajjadi, Saeed, Anand, Akrity, Beltrán, Ana M., Dvoranová, Dana, Boccaccini, Aldo R., Galusková, Dagmar, Jaška, David, Klement, Róbert, Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Universidad de Sevilla. TEP123: Metalurgia e Ingeniería de los Materiales, European Union's Horizon 2020 Research and Innovation Programme under grant agreement No 739566, Sajjadi, Saeed, Anand, Akrity, Beltrán, Ana M., Dvoranová, Dana, Boccaccini, Aldo R., Galusková, Dagmar, Jaška, David, and Klement, Róbert

Abstract

The Cu-doped mesoporous silica-based particles (Cu-BMS) were prepared using an evaporation-induced self-assembly sol-gel procedure as a heterogeneous catalyst for the activation of peroxydisulfate (PDS). The formation of well-organized mesoporous structures with amorphous nature and high surface area of 286 m²/g was demonstrated. The catalytic activity of Cu-BMS in the degradation of Methylene Blue (MB) and the effects of operating parameters, including Cu-BMS dosage, initial PDS amount, initial MB concentration, temperature and initial pH, were investigated in details. The Cu-BMS demonstrated a remarkable catalytic activity (93.5% degradation efficiency within 60 min) and good stability.

Published
2024

7. Unlocking the Potential of Iron-Containing Mesoporous Bioactive Glasses: Orchestrating Osteogenic Differentiation in Bone Marrow Mesenchymal Stem Cells and Osteoblasts

Author

Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Universidad de Sevilla. TEP123: Metalurgia e Ingeniería de los Materiales, Universidad de Sevilla, Zhou, Tian, Xu, Zeqian, Sun, Haishui, Beltrán, Ana M., Nawaz, Qaisar, Sui, Baiyan, Boccaccini, Aldo R., Zheng, Kai, Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Universidad de Sevilla. TEP123: Metalurgia e Ingeniería de los Materiales, Universidad de Sevilla, Zhou, Tian, Xu, Zeqian, Sun, Haishui, Beltrán, Ana M., Nawaz, Qaisar, Sui, Baiyan, Boccaccini, Aldo R., and Zheng, Kai

Abstract

Iron (Fe) is a crucial element in the human body, playing a significant role in bone metabolism. The release of Fe ions at bone defect sites can promote bone regeneration. In this study, we synthesized Fe-containing mesoporous bioactive glasses (Fe-MBGs) in SiO₂-CaO-Fe₂O₃ composition using a sol-gel method. Regardless of the amount of incorporated Fe₂O₃ (up to 5 mol%), the Fe-MBGs maintained a mesoporous structure, and the inclusion of Fe₂O₃ did not alter their amorphous characteristics. However, the presence of Fe₂O₃ led to a reduction in both pore volume and specific surface area of the Fe-MBGs. Notably, Fe-MBGs demonstrated degradability in physiological fluids and could sustain release of Si, Fe, and Ca ions. Higher concentrations of incorporated Fe₂O₃ were found to reduce the degradation of Fe-MBGs. All Fe-MBGs exhibited favorable bioactivity, as evidenced by the rapid formation of hydroxyapatite when exposed to simulated body fluid. Fe-MBGs also demonstrated concentration-dependent effects on BMSCs and Saos-2 cells. Extracts of Fe-MBGs at 0.1 and 1 mg/mL exhibited non-cytotoxicity and promoted cell proliferation. Additionally, extracts of Fe-MBGs at 1 mg/mL significantly enhanced the alkaline phosphatase activity of BMSCs and Saos-2 cells, along with an upregulation of the expression of osteogenesis-related genes. These findings unlock the significant potential of Fe-MBGs as functional biomaterials for bone regeneration applications. The controlled release of Fe ions from these mesoporous bioactive glasses orchestrates osteogenic differentiation in bone marrow mesenchymal stem cells and osteoblasts.

Published
2024

11. Contributors

Author

Asano, Takuji, primary, Baino, Francesco, additional, Boccaccini, Aldo R., additional, Deisinger, Ulrike, additional, Fiume, Elisa, additional, Gunduz, Oguzhan, additional, Hayakawa, Satoshi, additional, Kasuga, Toshihiro, additional, Katagiri, Kiyofumi, additional, Li, Huihua, additional, Mahirogullari, Mahir, additional, Mettang, Melanie, additional, Miyazaki, Toshiki, additional, Nakamura, Jin, additional, Narayan, Roger, additional, Nigoghossian, Karina, additional, Ohtsuki, Chikara, additional, Oktar, Faik Nuzhet, additional, Osaka, Akiyoshi, additional, Pezzotti, Giuseppe, additional, Piconi, Corrado, additional, Pitto, Rocco, additional, Porporati, Alessandro Alan, additional, Reinhardt, Carina, additional, Schuhladen, Katharina, additional, Shirosaki, Yuki, additional, Soga, Kohei, additional, Sugawara-Narutaki, Ayae, additional, Tafu, Masamoto, additional, Toshima, Takeshi, additional, Tsuru, Kanji, additional, Unal, Semra, additional, Wang, Min, additional, Wu, Jin-Ming, additional, Xia, Lunguo, additional, Yokoi, Taishi, additional, and Yoshioka, Tomohiko, additional

Published
2021
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13. Contributors

Author

Almaguer-Flores, Argelia, primary, Arango-Ospina, Marcela, additional, Augurio, Adriana, additional, Boccaccini, Aldo R., additional, Cazzola, Martina, additional, Drouet, Christophe, additional, Ferraris, Sara, additional, Gaspar, J., additional, Guarino, Vincenzo, additional, Huang, Jie, additional, Iafisco, Michele, additional, Massera, Jonathan, additional, Morra, Marco, additional, Narayan, Roger, additional, Nawaz, Qaisar, additional, Parisi, Ortensia Ilaria, additional, Pascale, Silvia, additional, Pires, L.R., additional, Puoci, Francesco, additional, Ravizza, Alice, additional, Rey, Christian, additional, Rodil, Sandra E., additional, Ruffo, Mariarosa, additional, Salama, Ahmed, additional, Serra, Tiziano, additional, Shukry, Nadia, additional, Silva-Bermúdez, Phaedra, additional, Spriano, Silvia, additional, Sutthavas, Pichaporn, additional, de Oliveira, Paulo Tambasco, additional, Tognato, Riccardo, additional, van Rijt, Sabine, additional, Warchomicka, Fernando, additional, Yamaguchi, Seiji, additional, Zengin, Aygul, additional, Zhang, Bin, additional, and Zuardi, Leonardo Raphael, additional

Published
2020
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16. Contributors

Author

Alotaibi, Hessah, primary, Arya, S.K., additional, Awais, Muhammad, additional, Bachar, Ahmed, additional, Baino, Francesco, additional, Boccaccini, Aldo R., additional, Borges, Roger, additional, Bretcanu, Oana, additional, Catteaux, Rémy, additional, Désanglois, Françoise, additional, Duée, Cédric, additional, Evans, Iain, additional, Farooq, Imran, additional, Ferraris, Sara, additional, Follet-Houttemane, Claudine, additional, Fu, Qiang, additional, Ghafoor, Faisal, additional, Gomez-Gramajo, Fátima, additional, Gorustovich, Alejandro, additional, Guo, Ya-Ping, additional, Haro Durand, Luis A., additional, Heise, Svenja, additional, Husain, Shehriar, additional, Hussain, Rafaqat, additional, Kai, Karen Cristina, additional, Kaur, Gurbinder, additional, Ke, Qin-Fei, additional, Khan, Maria, additional, Khan, Abdul S., additional, Khattak, Muhammad A., additional, Khurshid, Zohaib, additional, Krishnan, Vidya, additional, Kumar, Vishal, additional, Lebecq, Isabelle, additional, Lin, Yinan, additional, Lü, Jun-ying, additional, Marchi, Juliana, additional, Mauro, John C., additional, Mercier, Cyrille, additional, Miguez-Pacheco, Valentina, additional, Miola, Marta, additional, Najeeb, Shariq, additional, Nicholson, John, additional, Pickrell, Gary R., additional, Ramos Rivera, Laura, additional, Rehman, Rabiya, additional, Sefat, Farshid, additional, Stanić, Vojislav, additional, Syed, Mariam R., additional, Vargas, Gabriela E., additional, Vera-Mesones, Rosa, additional, Verné, Enrica, additional, Zafar, Muhammad S., additional, Zambanini, Telma, additional, and Zohaib, Sana, additional

Published
2019
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20. Cerium doped dendritic mesoporous bioactive glass nanoparticles with bioactivity and drug delivery capability

Author

Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Universidad de Sevilla. TEP123: Metalurgia e Ingeniería de los Materiales, Xu, Zhiyan, Keller, Emily, Beltrán, Ana M., Zheng, Kai, Boccaccini, Aldo R., Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Universidad de Sevilla. TEP123: Metalurgia e Ingeniería de los Materiales, Xu, Zhiyan, Keller, Emily, Beltrán, Ana M., Zheng, Kai, and Boccaccini, Aldo R.

Abstract

Dendritic mesoporous bioactive glass nanoparticles (DMBGNs) with unique three-dimensional structures are attracting increasing attention for biomedical applications. However, it is still challenging to tune the chemical composition of DMBGNs. In this work, we provide for the first time a feasible post-impregnation approach to incorporate metallic ions (Ca and Ce) into dendritic mesoporous silica nanoparticles, resulting in SiO₂-CaO and SiO₂-CaO-CeO₂ (Ce-DMBGNs) without destroying the dendritic mesoporous topography. Both DMBGNs and Ce-DMBGNs were amorphous, negatively charged, with a hydrodynamic size of ∼200 nm. The synthesized DMBGNs and Ce-DMBGNs exhibited high bioactivity as evidenced by the rapid formation of hydroxyapatite (HA) after immersion in simulated body fluid (SBF) for 7 days. In addition, DMBGNs and Ce-DMBGNs showed high propolis loading efficiency and sustained release behavior. The results suggest that the developed Ce-DMBGNs have the potential to be used as a delivery vehicle of therapeutic ions and drugs in bone regeneration applications.

Published
2023

21. Quaternary and pentanar mesoporous bioactive glass nanoparticles as novel nanocarriers for gallic acid: Characterisation, drug release and antibacterial activity

Author

Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Universidad de Sevilla. TEP123: Metalurgia e Ingeniería de los Materiales, Damian-Buda, Andrada-Ioana, Nawaz, Qaisar, Unalan, Irem, Beltrán, Ana M., Boccaccini, Aldo R., Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Universidad de Sevilla. TEP123: Metalurgia e Ingeniería de los Materiales, Damian-Buda, Andrada-Ioana, Nawaz, Qaisar, Unalan, Irem, Beltrán, Ana M., and Boccaccini, Aldo R.

Abstract

Mesoporous bioactive glass nanoparticles (MBGNs) have gained considerable attention as multifunctional platforms for simultaneously releasing ions and phytotherapeutic compounds. Thus, in the first part of this study, MBGNs based on the 53SiO₂–4P₂O₅–20CaO–23Na₂O (wt %) (S53P4) composition were synthesized by a microemulsion assisted sol-gel method. More precisely, P₂O₅ was substituted with B₂O₃ and Na₂O with MgO and/or ZnO. For B containing MBGNs all ions were successfully incorporated into the borosilicate structure without inducing crystallisation. In contrast, for S53P4 a poorly crystalline hydroxyapatite phase was identified. All MBGNs had a typical spherical shape with an internal radial network of mesopores. Additionally, for S53P4 a second fraction of particles with a smaller size and compact core was observed. Secondly, the feasibility of MBGNs as nanocarriers for gallic acid (GA) was evaluated. All drug-loaded samples showed a similar in vitro release profile which can be divided into three main phases: burst release, slow release and sustained release. Among the different compositions, S53P4 exhibited the highest cumulative release, whereas B and Mg containing particles exhibited the opposite. The presence of Zn in the MBGN compositions improved their antibacterial effect against both E. coli and S. aureus. Moreover, it was shown that depending on the MBGNs’ composition, the antibacterial activity of GA loaded MBGNs can be enhanced. Thus, the results proved that MBGNs can be used as controlled drug delivery system and, by tailoring the composition, a synergistic antibacterial effect can be achieved, considering that GA and biologically active ions are simultaneously released.

Published
2023

24. List of contributors

Author

Abhari, Roxanna Elizabeth, primary, Altobelli, Rosaria, additional, Álvarez-Pérez, Marco A., additional, Ambrosio, Luigi, additional, Basoli, Francesco, additional, Boccaccini, Aldo R., additional, Boffito, Monica, additional, Booth, Larnii, additional, Bushman, Jared, additional, Carella, Francesca, additional, Carr, Andrew Jonathan, additional, Castaño, Oscar, additional, Chanes-Cuevas, Osmar A., additional, Chavarría-Bolaños, Daniel, additional, Cheng, Liying, additional, Cheng, Ruoyu, additional, Chiellini, Federica, additional, Ciardelli, Gianluca, additional, Cirillo, Valentina, additional, Coppola, Sara, additional, Costantini, Marco, additional, Cruz Maya, Iriczalli, additional, Cui, Wenguo, additional, D’Amato, Anthony R., additional, Degli Esposti, Lorenzo, additional, Ferraro, Pietro, additional, Giannitelli, Sara M., additional, Gilbert, Ryan J., additional, Granados-Hernández, Marco V., additional, Grilli, Simonetta, additional, Guarino, Vincenzo, additional, Hernández-Tapia, Laura G., additional, Iafisco, Michele, additional, Johnson, Christopher D.L., additional, Kohn, Joachim, additional, Lanaro, Matthew, additional, Liverani, Liliana, additional, Mao, Xiyuan, additional, Martí-Muñoz, Joan, additional, Mouthuy, Pierre-Alexis, additional, Pires, Liliana R., additional, Powell, Sean K., additional, Pozos-Guillén, Amaury J., additional, Puppi, Dario, additional, Rainer, Alberto, additional, Serrano-Bello, Janeth, additional, Suarez-Franco, José L., additional, Sun, Xiaoming, additional, Tonda-Turo, Chiara, additional, Trombetta, Marcella, additional, Varesano, Alessio, additional, Vázquez-Vázquez, Febe C., additional, Vineis, Claudia, additional, Wan Abdul Khodir, W.K., additional, Woodruff, Maria A., additional, Zhang, Yuguang, additional, and Ziemba, Alexis M., additional

Published
2018
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25. Contributors

Author

Alves, S.A., primary, Aparicio, C., additional, Apaza-Bedoya, K., additional, Barão, V.A.R., additional, Beline, T., additional, Benfatti, C.A.M., additional, Bijukumar, D., additional, Bins-Ely, L., additional, Boccaccini, Aldo R., additional, Celis, J.-P., additional, Cooper, L., additional, da Silva, J.S.P., additional, Almeida Varela, H., additional, do Nascimento, R.M., additional, Fredel, M.C., additional, Galarraga-Vinueza, M.E., additional, Gindri, I.M., additional, Henriques, B., additional, Hotza, D., additional, Madeira, S., additional, Magini, R.S., additional, Mathew, M.T., additional, Mesquita-Guimarães, J., additional, Noronha Oliveira, M.A.P.P., additional, Novaes de Oliveira, A.P., additional, Pereira, J., additional, Piaia, L., additional, Pinto, N., additional, Quirynen, M., additional, Rocha, L.A., additional, Salmoria, G.V., additional, Shokuhfar, T., additional, Silva, F.S., additional, Sordi, M.B., additional, Souza, J.C.M., additional, Sukotjo, C., additional, Teughels, W., additional, and Zhang, Y., additional

Published
2018
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28. List of contributors

Author

Akbari, Zahra, primary, Almeida, Luis, additional, Almela, Thafar, additional, Amdjadi, Parisa, additional, Amrollahi, Pouya, additional, Ansari, Sahar, additional, Bader, Rizwan, additional, Baghaban Eslaminejad, Mohamadreza, additional, Bardsley, Katie, additional, Bencharit, Sompop, additional, Bindal, Priyadarshni, additional, Boccaccini, Aldo R., additional, Borkent, Dewi, additional, Brook, Ian, additional, Chai, Wen L., additional, Colley, Helen, additional, Crawford, Aileen, additional, Dabbagh, Ali, additional, Dashtimoghadarm, Erfan, additional, Dehghani, Shima, additional, Del Monico, Michael, additional, Dentino, Andrew R., additional, El-Bialy, Tarek, additional, El-Wassefy, Noha A., additional, Fahimipour, Farahnaz, additional, Fahmy, Mina D., additional, Faldu, Jasmine, additional, Farahani, Masomeh, additional, Farahat, Dina S., additional, Fatehinya, Atena, additional, Golzar, Hossein, additional, Gonzalez, Jose, additional, Goudouri, Ourania-Menti, additional, Hearnden, Vanessa, additional, Hopkinson, Louise, additional, Hosseini, Samaneh, additional, Ibrahim, Mohamed S., additional, Ivanovski, Saso, additional, Jafari, Maissa, additional, Jahangir, Shahrbanoo, additional, Jazayeri, Hossein E., additional, Abu Kasim, Noor H., additional, Khojasteh, Arash, additional, Khoshroo, Kimia, additional, Khurshid, Zohaib, additional, Kontonasaki, Eleana, additional, Lam, Lisetta, additional, Lee, Ryan S.B., additional, Mazafari, Masoud, additional, Moghadam, Farzaneh, additional, Moharamzadeh, Keyvan, additional, Moshaverinia, Alireza, additional, Murdoch, Craig, additional, Najeeb, Shariq, additional, Nazeman, Pantea, additional, Nejatian, Touraj, additional, Nicholson, Zach, additional, Omidi, Maysam, additional, Omidi, Meisam, additional, Paknejad, Zahrasadat, additional, Ramasamy, Thamil Selvee, additional, Rasoulianboroujeni, Morteza, additional, Rezai Rad, Maryam, additional, Salehi-Nik, Nasim, additional, Sefat, Farshid, additional, Seifi, Massoud, additional, Shah, Brinda, additional, Shahmoradi, Saleheh, additional, Tahriri, Mohammadreza, additional, Tayebi, Lobat, additional, Vashaee, Daryoosh, additional, Wirth, Jonathan, additional, Wu, Benjamin M., additional, Yadegari, Amir, additional, Yao, Wiley, additional, Yazdian, Fatemeh, additional, Yazdimamaghani, Mostafa, additional, S. Zafar, Muhammad, additional, Zahedi, Ehsan, additional, and Zohaib, Sana, additional

Published
2017
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29. Estudio in vitro de recubrimientos de vidrio bioactivo depositados mediante proyección térmica por plasma atmosférico

Author

Cañas Recacha, Eugeni, Grünewald, Alina, Detsch, Rainer, Orts Tarí, María José, Sánchez-Vilches, Enrique, and Boccaccini, Aldo R.

Subjects
cell culture test, proyección térmica por plasma atmosférico, atmospheric plasma spraying, simulated body fluid, fluido biológico simulado, bioactive glass coatings, polvo de vidrio bioactivo, recubrimiento de vidrio bioactivo, bioactive glass powder, ensayos de cultivo celular
Abstract

This research has addressed a complete study of the bioactivity of bioactive glass coatings obtained by atmospheric plasma spraying. The coatings have been characterized in terms of microstructure, adhesion, crystalline phases and bioactivity. Hydroxycarbonate apatite formation was also monitored following a standard protocol and the in vitro cell response was evaluated by human osteoblast-like cells (MG-63 cells) incubation. The obtained coatings shown a microstructure typical of glass coatings. A simulated body fluid test proved that coatings are capable of developing a surface layer of hydroxycarbonate apatite whereas the appearance of this phase takes place at a longer time than that observed for the powder feedstock. Cell-culture test showed multidirectional growth of MG-63 cells which promoted good contact between cells and the surface of the coating. This study has confirmed a positive effect of the coatings in terms of surface bioactivity and, more interestingly, it has proven an adequate cell-material interaction on the coating surface. Este trabajo ha abordado un estudio completo de la bioactividad de recubrimientos de vidrio bioactivo depositados mediante proyección térmica por plasma atmosférico. Se han caracterizado la microestructura, la adherencia, las fases cristalinas y la bioactividad de los recubrimientos obtenidos. También se ha estudiado la formación de hidroxiapatita carbonatada siguiendo un protocolo estándar y se ha evaluado la respuesta in vitro de los recubrimientos mediante su incubación con osteoblastos humanos (células MG-63). Los recubrimientos obtenidos han mostrado una microestructura típica de recubrimientos de vidrio. Tras la inmersión en fluido biológico simulado, se ha comprobado que el recubrimiento es capaz de desarrollar una capa superficial de hidroxiapatita carbonatada, aunque la velocidad de aparición de esta capa es menor que la observada para el polvo de vidrio de partida. El ensayo de cultivo celular ha mostrado un crecimiento multidireccional de las células MG-63, dando lugar a un buen contacto entre las células y la superficie del recubrimiento. Este estudio ha confirmado un efecto positivo de los recubrimientos en términos de bioactividad de la superficie y, lo que es más interesante, ha demostrado una adecuada interacción célula-material sobre la superficie del recubrimiento.

Published
2022

31. Preface

Author

Boccaccini, Aldo R., primary and Ma, Peter X., additional

Published
2014
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32. Deposition of bioactive gelatin coatings on porous titanium: Influence of processing parameters, size and pore morphology

Author

Torres Hernández, Yadir, Begines Ruiz, Belén, Beltrán, Ana M., Boccaccini, Aldo R., Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica, Universidad de Sevilla. TEP123: Metalurgia e Ingeniería de los Materiales, and Universidad de Sevilla. FQM135: Carbohidratos y Polímeros

Subjects
Osteochondral defects, Tribomechanical behavior, Biphasic implant, Structural characterization, Porous titanium substrates, Gelatin coating
Abstract

In this work porous commercially pure Ti substrates fabricated by spacer-holder technique (50 vol% NH4HCO3 and three range pores sizes, 100–200 μm, 250–355 μm and 355–500 μm) were coated with two types of gelatinous materials, one as a linear polymer (non-crosslinked) and another one crosslinked. The role of the crosslink, as well as the morphology, size and degree of interconnectivity of gelatins coatings on their tribo mechanical behavior (P-h curves and scratch tests) were analyzed. Results revealed a new promising route to manufacture composite porous implants which are potential candidates to develop new treatments, not only for osteochondral defects, but also for other types of damages involving tissues of different nature (hard and soft) perfectly joined. Ministry of Science and Innovation of Spain PID2019-109371GB-I00

Published
2021

34. List of Contributors

Author

Abu-Lail, Nehal I., primary, Bandyopadhyay, Amit, additional, Beyenal, Haluk, additional, Biswas, Subhasish, additional, Boccaccini, Aldo R., additional, Bose, Susmita, additional, Cao, Hengchu, additional, Chu, Paul K., additional, Detsch, Rainer, additional, Gupta, Gautam, additional, Khan, Imran, additional, Sampath Kumar, T.S., additional, Nandi, Samit K., additional, Naylor, Malcolm, additional, Roeder, Ryan K., additional, Roy, Mangal, additional, Sharma, Chandra P., additional, Thasneem, Y.M., additional, Wang, Huaiyu, additional, Will, Julia, additional, and Wu, Ming H., additional

Published
2013
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35. Contributor contact details

Author

Banerjee, Rajat, primary, Manna, Indranil, additional, Ranjan Bose, Nripati, additional, Dutta Majumder, D., additional, Dutta Majumder, Debosmita, additional, Karan, Sankar, additional, Banerjee, Rajat, additional, Hvizdoš, Pavol, additional, Tomar, Vikas, additional, He, Fei, additional, Boccaccini, Aldo R., additional, Subhani, Tayyab, additional, Shaffer, Milo S.P., additional, Liang, Xinhua, additional, King, David M., additional, Weimer, Alan W., additional, Tonoyan, Anahit O., additional, Davtyan, S.P., additional, Paul, Gayatri, additional, Wieczorek-Ciurowa, Krystyna, additional, Fang, Zak, additional, Dubois, Philippe, additional, Kumar, Binod, additional, Garmendia, Nere, additional, and Okamoto, Masami, additional

Published
2013
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36. Contributor contact details

Author

Purbrick, Malcolm, primary, Ambrosio, Luigi, additional, Ventre, Maurizio, additional, Netti, Paolo, additional, Cardon, Ludwig K., additional, Ragaert, K.J., additional, Koster, R.P., additional, Tanner, K. Elizabeth, additional, Santin, Matteo, additional, Grøndahl, Lisbeth, additional, Jack, Kevin, additional, Wang, Min, additional, Gloria, Antonio, additional, De Santis, Roberto, additional, Causa, Filippo, additional, Nazhat, Showan N., additional, Deb, Sanjukta, additional, Weiss, Pierre, additional, Fatimi, Ahmed, additional, Di Benedetto, A.T., additional, Pinatti, Laura, additional, Anderson, James M., additional, Voskerician, Gabriela, additional, Jayakumar, Prakash, additional, Di Silvio, Lucy, additional, Giardino, Roberto, additional, Fini, Milena, additional, Aldini, Nicolò Nicoli, additional, Parrilli, Annapaola, additional, Nicolais, Luigi, additional, Kanagaraj, S., additional, Oliveira, Monica S.A., additional, de Oliveira Simões, José António, additional, Pegoretti, Alessandro, additional, Meng, Decheng, additional, Boccaccini, Aldo R., additional, Tampieri, Anna, additional, Sprio, Simone, additional, Landi, Elena, additional, Sandri, Monica, additional, Planell, Josep A, additional, Navarro, Melba, additional, Trommelmans, Leen, additional, and Dierickx, Kris, additional

Published
2010
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37. Contributor contact details

Author

Boccaccini, Aldo R., primary, Gough, Julie E., additional, Huang, J., additional, Best, S.M., additional, Wei, Guobao, additional, Ma, Peter X., additional, Jones, J.R., additional, Misra, S.K., additional, Boccaccini, A.R., additional, Mansbridge, J., additional, Shakesheff, K., additional, Tsourpas, G., additional, Tirelli, N., additional, Cellesi, F., additional, Davis, F., additional, Higson, S.P.J., additional, Urquhart, Andrew J., additional, Alexander, Morgan R., additional, Donald, A.M., additional, Anderson, S.I., additional, Notingher, I., additional, Atala, A., additional, Woźniak, P., additional, El Haj, A.J., additional, Di Silvio, L., additional, Chen, Q.Z., additional, Jawad, H., additional, Harding, S.E., additional, Ali, N.N., additional, Hoyland, J., additional, Freemont, T., additional, MacNeil, S., additional, Saito, A., additional, Turner, A.M., additional, Southgate, J., additional, Subramaniam, R., additional, Thomas, D.F.M., additional, Kingham, P., additional, Terenghi, G., additional, Bishop, A.E., additional, Rippon, H.J., additional, Lloyd, D.A.J., additional, Gabe, S.M., additional, Fritsch, A., additional, Dormieux, L., additional, Hellmich, C., additional, Sanahuja, J., additional, and Gough, J.E., additional

Published
2007
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39. Metals

Author

MINAY, E. JANE, primary and BOCCACCINI, ALDO R., additional

Published
2005
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40. Preface: Vitrification and geopolymerization of industrial wastes

Author

Rincón, Jesús María, Jordán, Manuel, Boccaccini, Aldo R., Rincón, Jesús María, Jordán, Manuel, and Boccaccini, Aldo R.

Abstract

This VIRTUAL TOPICAL ISSUE of MATERIALS LETTERS including 35 refereed letters from the international community is the response to a broad Open Call for papers published in 2017, which attracted contributions from Europe (Bulgaria, France, Germany, Italy, Poland, Portugal, Spain and United Kingdom), Algeria, Argentina, Bolivia, Brazil, China, Colombia, Ecuador and Ukraine. The idea of publishing this topical issue in Materials Letters originated from the success of a scientific meeting supported by the Technical Committee TC05 of the International Commission on Glass (ICG), focused on Industrial Wastes and their Vitrification, which was held at University ‘‘Miguel Hernández” in Elche, Alicante, Spain, in September 2017 under the general topic: ‘‘Vitrification and Geopolymerization of Industrial Wastes”. The scope of that ‘‘pioneering” meeting and indeed of this topical issue has been to increase the interactions and participation of scientists involved in glasses from wastes (vitrification processing) and those involved in geopolymers (alkali activation) from wastes, e.g. in both cases developing fundamental science and technologies to achieve the inertization or safe encapsulation of wastes in inorganic matrices. In both cases, the disordered nature of structures (amorphous or pseudo-amorphous) facilitates the hosting or immobilization of a wide range of industrial wastes. However, there are questions not yet fully solved such as: the proportion of ‘‘short or large range ordering” in both these types of materials; their capability, commonalities and differences to immobilize specific type of residues; the determination of the most efficient treatment by vitrification or geo-polymerization; the use of glasses and waste glasses as precursors for geopolymer production, among other open research topics.

Published
2019

41. Bioactive Coatings on Porous Titanium for Biomedical Applications

Author

Domínguez-Trujillo, Cristina, Ternero Fernández, Fátima, Rodríguez-Ortiz, José Antonio, Heise, S., Boccaccini, Aldo R., Lebrato Martínez, Julián, Torres Hernández, Yadir, Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Universidad de Sevilla. Departamento de Ingeniería Química, Universidad de Sevilla. TEP123: Metalurgia e Ingeniería de los Materiales, and Universidad de Sevilla. RNM159: Grupo TAR-Bioingeniería

Subjects
Porous titanium, Osseointegration, Bioactive glass coating, Stress-shielding, In vitro bioactivity, Hydroxyapatite
Abstract

Commercial pure titanium is a recognized and accepted material for cortical bone tissue substitution. However, stress-shielding phenomena and lack of osseointegration result in significant limitations. This work is focused on the achievement of an effective solution for both problems via fabrication of porous titanium substrates coated with bioactive glass. Substrates were obtained through the space holder technique giving values of stiffness and yield strength compatible with cortical bone tissue to reduce the stress-shielding phenomenon. Titanium substrates were coated with different number of layers of bioactive glass 45S5 by dripping sedimentation. The substrates porosity was characterized by different techniques. Ultrasound, compression and micro-mechanical testing were used for mechanical properties evaluation. After substrates coating, the infiltration ability, coating homogeneity and structural integrity (chipping and cracking) were evaluated for each coating layer. The chemical composition of coating and phases were studied before and after in vitro tests in Simulated Body Fluid. The results showed more homogenous coating, adherence and greater hydroxyapatite growth for the tri-layer system in both dense and porous samples. Besides, the relation of Ca/P was closed to that of stoichiometric hydroxyapatite in the human body. The coated porous titanium could be potentially used in load bearing partial implants with improved osseointegration. Ministry of Economy and Competitiveness of the State General Administration of Spain grant MAT2015-71284-P

Published
2018

42. Bioactive Coatings on Porous Titanium for Biomedical Applications

Author

Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Universidad de Sevilla. Departamento de Ingeniería Química, Universidad de Sevilla. TEP123: Metalurgia e Ingeniería de los Materiales, Universidad de Sevilla. RNM159: Grupo TAR-Bioingeniería, Domínguez-Trujillo, Cristina, Ternero Fernández, Fátima, Rodríguez-Ortiz, José Antonio, Heise, S., Boccaccini, Aldo R., Lebrato Martínez, Julián, Torres Hernández, Yadir, Universidad de Sevilla. Departamento de Ingeniería y Ciencia de los Materiales y del Transporte, Universidad de Sevilla. Departamento de Ingeniería Química, Universidad de Sevilla. TEP123: Metalurgia e Ingeniería de los Materiales, Universidad de Sevilla. RNM159: Grupo TAR-Bioingeniería, Domínguez-Trujillo, Cristina, Ternero Fernández, Fátima, Rodríguez-Ortiz, José Antonio, Heise, S., Boccaccini, Aldo R., Lebrato Martínez, Julián, and Torres Hernández, Yadir

Abstract

Commercial pure titanium is a recognized and accepted material for cortical bone tissue substitution. However, stress-shielding phenomena and lack of osseointegration result in significant limitations. This work is focused on the achievement of an effective solution for both problems via fabrication of porous titanium substrates coated with bioactive glass. Substrates were obtained through the space holder technique giving values of stiffness and yield strength compatible with cortical bone tissue to reduce the stress-shielding phenomenon. Titanium substrates were coated with different number of layers of bioactive glass 45S5 by dripping sedimentation. The substrates porosity was characterized by different techniques. Ultrasound, compression and micro-mechanical testing were used for mechanical properties evaluation. After substrates coating, the infiltration ability, coating homogeneity and structural integrity (chipping and cracking) were evaluated for each coating layer. The chemical composition of coating and phases were studied before and after in vitro tests in Simulated Body Fluid. The results showed more homogenous coating, adherence and greater hydroxyapatite growth for the tri-layer system in both dense and porous samples. Besides, the relation of Ca/P was closed to that of stoichiometric hydroxyapatite in the human body. The coated porous titanium could be potentially used in load bearing partial implants with improved osseointegration.

Published
2018

43. Extensive reuse of soda-lime waste glass in fly ash-based geopolymers

Author

Toniolo, Nicoletta, Rincon Romero, Acacio, Roether, J.A., Ercole, Piero, Bernardo, Enrico, Boccaccini, Aldo R., Toniolo, Nicoletta, Rincon Romero, Acacio, Roether, J.A., Ercole, Piero, Bernardo, Enrico, and Boccaccini, Aldo R.

Abstract

The possibility of extensive incorporation of soda-lime waste glass in the synthesis of fly ash-based geopolymers was investigated. Using waste glass as silica supplier avoids the use of water glass solution as chemical activator. The influence of the addition of waste glass on the microstructure and strength of fly ash-based geopolymers was studied through microstructural and mechanical characterization. Leaching analyses were also carried out. The samples were developed changing the SiO2/Al2O3 molar ratio and the molarity of the sodium hydroxide solution used as alkaline activator. The results suggest that increasing the amount of waste glass as well as increasing the molarity of the solution lead to the formation of zeolite crystalline phases and an improvement of the mechanical strength. Leaching results confirmed that the new geopolymers have the capability to immobilize heavy metal ions.

Published
2018

44. In silico design of magnesium implants: Macroscopic modelling

Author

Universidad de Sevilla. Departamento de Mecánica de Medios Continuos y Teoría de Estructuras, Universidad de Sevilla. TEP245: Ingeniería de las Estructuras, Ministerio de Economía y Competitividad (MINECO). España, Sanz Herrera, José Antonio, Reina Romo, Esther, Boccaccini, Aldo R., Universidad de Sevilla. Departamento de Mecánica de Medios Continuos y Teoría de Estructuras, Universidad de Sevilla. TEP245: Ingeniería de las Estructuras, Ministerio de Economía y Competitividad (MINECO). España, Sanz Herrera, José Antonio, Reina Romo, Esther, and Boccaccini, Aldo R.

Abstract

Magnesium-based biomedical implants offer many advantages versus traditional ones although some challenges are still present. In this context, mathematical modeling and computational simulation may be a useful and complementary tool to evaluate in silico the performance of magnesium biomaterials under different conditions. In this paper, a phenomenologically-based model to simulate magnesium corrosion is developed. The model describes the physico-chemical interactions and evolution of species present in this phenomenon. A set of 7 species is considered in the model, which allows to simulate hydrogen release, pH evolution, corrosion products formation as well as degradation of magnesium. The model is developed under the continuum media theory and is implemented in a finite element framework. In the results section, the effect of model parameters on outcomes is firstly explored. Second, model results are qualitative validated versus two examples of application found in the literature. Two main conclusions are derived from this work: (i) the model captures well the experimental trends and allows to analyze the main variables present in magnesium corrosion, (ii) even though further validation is needed the model may be a useful standard in the design of degradable metal implants.

Published
2018

45. Sponge-derived natural bioactive glass microspheres with self-assembled surface channel arrays opening into a hollow core for bone tissue and controlled drug release applications

Author

Martin V. Sørensen, Ebru Toksoy Oner, Ilker Torun, Lalehan Akyuz, Idris Sargin, Merve Erginer Hasköylü, Muhammad Mujtaba, Aldo R. Boccaccini, Demet Cansaran-Duman, Ismail Bilican, Caglar Elbuken, Kai Zheng, M. Serdar Onses, Murat Kaya, Kaya, Murat, Bilican, Ismail, Mujtaba, Muhammad, Sargin, Idris, Haskoylu, Merve Erginer, Oner, Ebru Toksoy, Zheng, Kai, Boccaccini, Aldo R., Cansaran-Duman, Demet, Onses, M. Serdar, Torun, Ilker, Akyuz, Lalehan, Elbuken, Caglar, Sorensen, Martin Vinther, Rektörlük, and Elbüken, Çağlar

Subjects
Materials science, Bone tissue, Silica fume, General Chemical Engineering, Simulated body fluid, FABRICATION, 02 engineering and technology, SPICULES, 010402 general chemistry, SCAFFOLDS, 01 natural sciences, Industrial and Manufacturing Engineering, Biosilica, law.invention, SDG 3 - Good Health and Well-being, law, Porous beads, NANOPARTICLES, medicine, Environmental Chemistry, ENCAPSULATION, SILICA, Bone regeneration, Porosity, Nanoscopic scale, Macroporous silica, General Chemistry, Drug release, CHEMOTHERAPY, 021001 nanoscience & nanotechnology, HYDROXYAPATITE, POLYMERIC MICELLES, 0104 chemical sciences, Porifera, medicine.anatomical_structure, Chemical engineering, Bioactive glass, Drug delivery, Geodia, 0210 nano-technology, Sterrasters
Abstract

*Bilican, Ismail ( Aksaray, Yazar ), Porous, bioactive microspheres have always been a dream material to biomedical scientists for bone regeneration and drug delivery applications due to their interconnectivity, unique pore geometry, encapsulation ability and porosity spanning macroscopic, microscopic and nanoscopic length scales. Extensive efforts have been made to produce such materials synthetically at a great cost of money, time and labor. Herein, naturally-assembled multifunctional, open-channeled and hollow bioactive micro silica spheres (diameter 209.4 +/- 38.5 mu m) were discovered in a marine sponge (Geodia macandrewii), by peeling the outer surface of the sterrasters using hydrogen fluoride. The obtained micro silica spheres exhibited valuable characteristics such as homogeneously distributed pores, a cavity in the center of the sphere, and channels (approx. 3000) opening from each pore into the central cavity. Simulated body fluid analysis demonstrated the bioactivity of the micro silica spheres; whereas, no bioactivity was recorded for the original untreated sterrasters. The non-cytotoxicity and osteogenic ability of the isolated microspheres were confirmed through osteoblast cell culture. Finally, these silica based porous microspheres were tested for controlled drug release capacity. The spheres showed excellent loading and release abilities for an anti-cancer drug, carboplatin, in simulated solutions and in human cancer cell culture, HeLa, through a real time cell analyzer system. The drug loading capacity of the porous beads was determined as 10.59%. Considering the unique biological and physicochemical properties, these novel bioactive silica spheres, which we name as giant macroporous silica (GMS), are promising materials for a range of applications including bone tissue engineering and drug delivery.

Published
2021

46. Multifunctional scaffolds for bone tissue engineering and in situ drug delivery

Author

Juan Pablo Cattalini, Viviana Silvia Lourdes Mouriño, Aldo R. Boccaccini, Silvia Edith Lucangioli, Wei Li, and Boccaccini, Aldo R.

Subjects
BONE TISSUE ENGINEERING, DRUG DELIVERY, Materials science, THERAPEUTIC DRUGS, Otras Ingeniería de los Materiales, INGENIERÍAS Y TECNOLOGÍAS, Bone tissue, Bone tissue engineering, MULTIFUNCTIONAL SCAFFOLDS, medicine.anatomical_structure, Ingeniería de los Materiales, Controlled delivery, Drug delivery, medicine, Biomedical engineering
Abstract

This chapter provides an overview about the development of bone tissue engineering scaffolds with capability for the controlled delivery of therapeutic drugs. Typical drugs considered include gentamicin and other antibiotics generally used to combat osteomyelitis as well as anti-inflammatory drugs and bisphosphonates. Special attention has been given to the technology used for controlling the release of the loaded drugs. A detailed summary of drugs included in bone tissue scaffolds is presented and the several approaches developed to combine organic and inorganic biomaterials in composites for drug delivery systems are discussed. The remaining challenges in the field are summarized suggesting also future research directions. Fil: Mouriño, Viviana Silvia Lourdes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina Fil: Cattalini, Juan Pablo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina Fil: Wei, L.. No especifíca; Fil: Boccaccini, A. R.. No especifíca; Fil: Lucangioli, Silvia Edith. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Tecnología Farmacéutica; Argentina

Published
2014

47. Electrically conductive coatings in tissue engineering.

Author

Kohestani AA, Xu Z, Baştan FE, Boccaccini AR, and Pishbin F

Subjects
Humans, Animals, Polymers chemistry, Tissue Engineering methods, Electric Conductivity, Coated Materials, Biocompatible chemistry, Coated Materials, Biocompatible pharmacology, Tissue Scaffolds chemistry
Abstract

Recent interest in tissue engineering (TE) has focused on electrically conductive biomaterials. This has been inspired by the characteristics of the cells' microenvironment where signalling is supported by electrical stimulation. Numerous studies have demonstrated the positive influence of electrical stimulation on cell excitation to proliferate, differentiate, and deposit extracellular matrix. Even without external electrical stimulation, research shows that electrically active scaffolds can improve tissue regeneration capacity. Tissues like bone, muscle, and neural contain electrically excitable cells that respond to electrical cues provided by implanted biomaterials. To introduce an electrical pathway, TE scaffolds can incorporate conductive polymers, metallic nanoparticles, and ceramic nanostructures. However, these materials often do not meet implantation criteria, such as maintaining mechanical durability and degradation characteristics, making them unsuitable as scaffold matrices. Instead, depositing conductive layers on TE scaffolds has shown promise as an efficient alternative to creating electrically conductive structures. A stratified scaffold with an electroactive surface synergistically excites the cells through active top-pathway, with/without electrical stimulation, providing an ideal matrix for cell growth, proliferation, and tissue deposition. Additionally, these conductive coatings can be enriched with bioactive or pharmaceutical components to enhance the scaffold's biomedical performance. This review covers recent developments in electrically active biomedical coatings for TE. The physicochemical and biological properties of conductive coating materials, including polymers (polypyrrole, polyaniline and PEDOT:PSS), metallic nanoparticles (gold, silver) and inorganic (ceramic) particles (carbon nanotubes, graphene-based materials and Mxenes) are examined. Each section explores the conductive coatings' deposition techniques, deposition parameters, conductivity ranges, deposit morphology, cell responses, and toxicity levels in detail. Furthermore, the applications of these conductive layers, primarily in bone, muscle, and neural TE are considered, and findings from in vitro and in vivo investigations are presented. STATEMENT OF SIGNIFICANCE: Tissue engineering (TE) scaffolds are crucial for human tissue replacement and acceleration of healing. Neural, muscle, bone, and skin tissues have electrically excitable cells, and their regeneration can be enhanced by electrically conductive scaffolds. However, standalone conductive materials often fall short for TE applications. An effective approach involves coating scaffolds with a conductive layer, finely tuning surface properties while leveraging the scaffold's innate biological and physical support. Further enhancement is achieved by modifying the conductive layer with pharmaceutical components. This review explores the under-reviewed topic of conductive coatings in tissue engineering, introducing conductive biomaterial coatings and analyzing their biological interactions. It provides insights into enhancing scaffold functionality for tissue regeneration, bridging a critical gap in current literature., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.)

Published
2024
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48. Architecture of β-lactoglobulin coating modulates bioinspired alginate dialdehyde-gelatine/polydopamine scaffolds for subchondral bone regeneration.

Author

Ghorbani F, Kim M, Ghalandari B, Zhang M, Varma SN, Schöbel L, Liu C, and Boccaccini AR

Subjects
Animals, Sheep, Mesenchymal Stem Cells metabolism, Mesenchymal Stem Cells drug effects, Mesenchymal Stem Cells cytology, Aldehydes chemistry, Cell Proliferation drug effects, Alginates chemistry, Alginates pharmacology, Indoles chemistry, Indoles pharmacology, Tissue Scaffolds chemistry, Polymers chemistry, Polymers pharmacology, Bone Regeneration drug effects, Gelatin chemistry, Lactoglobulins chemistry, Lactoglobulins pharmacology, Coated Materials, Biocompatible pharmacology, Coated Materials, Biocompatible chemistry
Abstract

In this study, we developed polydopamine (PDA)-functionalized alginate dialdehyde-gelatine (ADA-GEL) scaffolds for subchondral bone regeneration. These polymeric scaffolds were then coated with β-Lactoglobulin (β-LG) at concentrations of 1 mg/ml and 2 mg/ml. Morphological analysis indicated a homogeneous coating of the β-LG layer on the surface of network-like scaffolds. The β-LG-coated scaffolds exhibited improved swelling capacity as a function of the β-LG concentration. Compared to ADA-GEL/PDA scaffolds, the β-LG-coated scaffolds demonstrated delayed degradation and enhanced biomineralization. Here, a lower concentration of β-LG showed long-lasting stability and superior biomimetic hydroxyapatite mineralization. According to the theoretical findings, the single-state, representing the low concentration of β-LG, exhibited a homogeneous distribution on the surface of the PDA, while the dimer-state (high concentration) displayed a high likelihood of uncontrolled interactions. β-LG-coated ADA-GEL/PDA scaffolds with a lower concentration of β-LG provided a biocompatible substrate that supported adhesion, proliferation, and alkaline phosphatase (ALP) secretion of sheep bone marrow mesenchymal stem cells, as well as increased expression of osteopontin (SPP1) and collagen type 1 (COL1A1) in human osteoblasts. These findings indicate the potential of protein-coated scaffolds for subchondral bone tissue regeneration. STATEMENT OF SIGNIFICANCE: This study addresses a crucial aspect of osteochondral defect repair, emphasizing the pivotal role of subchondral bone regeneration. The development of polydopamine-functionalized alginate dialdehyde-gelatine (ADA-GEL) scaffolds, coated with β-Lactoglobulin (β-LG), represents a novel approach to potentially enhance subchondral bone repair. β-LG, a milk protein rich in essential amino acids and bioactive peptides, is investigated for its potential to promote subchondral bone regeneration. This research explores computationally and experimentally the influence of protein concentration on the ordered or irregular deposition, unravelling the interplay between coating structure, scaffold properties, and in-vitro performance. This work contributes to advancing ordered protein coating strategies for subchondral bone regeneration, providing a biocompatible solution with potential implications for supporting subsequent cartilage repair., Competing Interests: Declaration of competing interest The authors whose names are listed certify that they have NO affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers' bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript., (Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Published
2024
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49. Bovine serum albumin-modified 3D printed alginate dialdehyde-gelatin scaffolds incorporating polydopamine/SiO 2 -CaO nanoparticles for bone regeneration.

Author

Kim M, Schöbel L, Geske M, Boccaccini AR, and Ghorbani F

Subjects
Humans, Alginates chemistry, Gelatin chemistry, Serum Albumin, Bovine, Silicon Dioxide, Bone Regeneration, Printing, Three-Dimensional, Tissue Engineering methods, Osteogenesis, Tissue Scaffolds chemistry, Nanoparticles, Indoles, Polymers
Abstract

Three-dimensional (3D) printing allows precise manufacturing of bone scaffolds for patient-specific applications and is one of the most recently developed and implemented technologies. In this study, bilayer and multimaterial alginate dialdehyde-gelatin (ADA-GEL) scaffolds incorporating polydopamine (PDA)/SiO 2 -CaO nanoparticle complexes were 3D printed using a pneumatic extrusion-based 3D printing technology and further modified on the surface with bovine serum albumin (BSA) for application in bone regeneration. The morphology, chemistry, and in vitro bioactivity of PDA/SiO 2 -CaO nanoparticle complexes were characterized (n = 3) and compared with those of mesoporous SiO 2 -CaO nanoparticles. Successful deposition of the PDA layer on the surface of the SiO 2 -CaO nanoparticles allowed better dispersion in a liquid medium and showed enhanced bioactivity. Rheological studies (n = 3) of ADA-GEL inks consisting of PDA/SiO 2 -CaO nanoparticle complexes showed results that may indicate better injectability and printability behavior compared to ADA-GEL inks incorporating unmodified nanoparticles. Microscopic observations of 3D printed scaffolds revealed that PDA/SiO 2 -CaO nanoparticle complexes introduced additional topography onto the surface of 3D printed scaffolds. Additionally, the modified scaffolds were mechanically stable and elastic, closely mimicking the properties of natural bone. Furthermore, protein-coated bilayer scaffolds displayed controllable absorption and biodegradation, enhanced bioactivity, MC3T3-E1 cell adhesion, proliferation, and higher alkaline phosphatase (ALP) activity (n = 3) compared to unmodified scaffolds. Consequently, the present results confirm that ADA-GEL scaffolds incorporating PDA/SiO 2 -CaO nanoparticle complexes modified with BSA offer a promising approach for bone regeneration applications., Competing Interests: Declaration of competing interest The authors whose names are listed certify that they have no affiliations with or involvement in any organization or entity with any financial or non-financial interest in the subject matter or materials discussed in this manuscript., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Published
2024
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50. Tailorable mechanical and degradation properties of KCl-reticulated and BDDE-crosslinked PCL/chitosan/κ-carrageenan electrospun fibers for biomedical applications: Effect of the crosslinking-reticulation synergy.

Author

Vargas-Osorio Z, González Castillo EI, Mutlu N, Vidomanová E, Michálek M, Galusek D, and Boccaccini AR

Subjects
Carrageenan, Tissue Engineering methods, Polyesters, Polymers, Solvents, Organic Chemicals, Tissue Scaffolds, Chitosan
Abstract

The development of intricated and interconnected porous mats is desired for many applications in biomedicine and other relevant fields. The mats that comprise the use of natural, bioactive, and biodegradable polymers are the focus of current research activities. In the present work, crosslinked fibers with improved characteristics were produced by incorporating 1,4-butanediol diglycidyl ether (BDDE) into a polymer formulation containing polycaprolactone (PCL), chitosan (CS), and κappa-carrageenan (κ-C). A slight variation of formic acid (FA)/acetic acid (AA) ratio used as a solvent system, significantly affected the characteristics of the produced fiber mats. Both polysaccharides and BDDE played a major role in tailoring mechanical properties when fibrous scaffolds were reticulated under KCl-mediated basic conditions for determined periods of time at 50 °C. In vitro biological assessment of the electrospun fiber mats revealed proliferation of MC3T3-E1 cells when incubated for 1 and 7 days. After staining the cells with 4',6-diamidino-2-phenylindole (DAPI)/rhodamine phalloidin an autofluorescence response was observed by fluorescence microscopy in the scaffold manufactured using a solvent with higher FA/AA ratio due to the formation of microfibers. The results demonstrated the potential of the BDDE-crosslinked PCL/CS/κ-C electrospun fibers as promising materials for biomedical applications that may include soft and bone tissue regeneration., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Published
2024
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