1. Impact of bone marrow-derived mesenchymal stromal cells on experimental xenogeneic graft-versus-host disease.
- Author
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Bruck F, Belle L, Lechanteur C, de Leval L, Hannon M, Dubois S, Castermans E, Humblet-Baron S, Rahmouni S, Beguin Y, Briquet A, and Baron F
- Subjects
- Animals, Bone Marrow Cells cytology, Graft vs Host Disease etiology, Graft vs Host Disease genetics, Humans, Leukocytes, Mononuclear transplantation, Mice, Mice, SCID, T-Lymphocytes immunology, T-Lymphocytes transplantation, Transplantation, Homologous adverse effects, Graft vs Host Disease physiopathology, Mesenchymal Stem Cell Transplantation, Mesenchymal Stem Cells cytology
- Abstract
Background Aims: Graft-versus-host disease (GVHD) is a life-threatening complication of allogeneic hematopoietic cell transplantation caused by donor T cells reacting against host tissues. Previous studies have suggested that mesenchymal stromal cells (MSCs) could exert potent immunosuppressive effects., Methods: The ability of human bone marrow derived MSCs to prevent xenogeneic GVHD in non-obese diabetic/severe combined immunodeficient (NOD/SCID) mice and in NOD/SCID/interleukin-2Rγ(null) (NSG) mice transplanted with human peripheral blood mononuclear cells (PBMCs) was assessed., Results: Injection of 200 × 10(6) human PBMCs intraperitoneally (IP) into sub-lethally (3.0 Gy) irradiated NOD/SCID mice also given anti-asialo GM1 antibodies IP 1 day prior and 8 days after transplantation induced lethal xenogeneic GVHD in all tested mice. Co-injection of 2 × 10(6) MSCs IP on day 0 did not prevent lethal xenogeneic GVHD induced by injection of human PBMCs. Similarly, injection of 30 × 10(6) human PBMCs IP into sub-lethally (2.5 Gy) irradiated NSG mice induced a lethal xenogeneic GVHD in all tested mice. Injection of 3 × 10(6) MSCs IP on days 0, 7, 14 and 21 did not prevent lethal xenogeneic GVHD induced by injection of human PBMCs., Conclusions: Injection of MSCs did not prevent xenogeneic GVHD in these two humanized mice models., (Copyright © 2013 International Society for Cellular Therapy. Published by Elsevier Inc. All rights reserved.)
- Published
- 2013
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