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Soil microbial communities play a vital role in the biogeochemical cycling and ecological functioning of grassland, but may be affected by common land uses such as cattle grazing. Changes in microbial diversity and network complexity can affect key ecosystem functions such as nutrient cycling. However, it is not well known how microbial diversity and network complexity respond to grazing in the Northern Great Plains. Consequently, it is important to understand whether variation in grazing management alters the diversity and complexity of grassland microbial communities. We compared the effect of intensive adaptive multi-paddock (AMP) grazing and conventional grazing practices on soil microbial communities using 16S/ITS amplicon sequencing. Samples were collected from grasslands in 13 AMP ranches and 13 neighboring, conventional ranches located across the Canadian prairies. We found that AMP grazing increased fungal diversity and evenness, and led to more complex microbial associations. Acidobacteria, Actinobacteria, Gemmatimonadetes, and Bacteroidetes were keystone taxa associated with AMP grazing, while Actinobacteria, Acidobacteria, Proteobacteria, and Armatimonadetes were keystone taxa under conventional grazing. Besides overall grazing treatment effects, specific grazing metrics like cattle stocking rate and rest-to-grazing ratio affected microbial richness and diversity. Bacterial and fungal richness increased with elevated stocking rate, and fungal richness and diversity increased directly with the rest-to-grazing ratio. These results suggest that AMP grazing may improve ecosystem by enhancing fungal diversity and increasing microbial network complexity and connectivity., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)

Grasslands are globally abundant and provide many ecosystem services, including carbon (C) storage. While grasslands are widely subject to livestock grazing, the influence of grazing on grassland ecosystem C remains unclear. We studied the effect of long-term livestock grazing on C densities of different ecosystem components in 110 northern temperate grasslands across a broad agroclimatic gradient in Alberta, Canada. These grasslands stored 50 to 180 t ha -1 C in live and dead vegetation, as well as soil C to 30 cm depth, with the majority as soil organic C (SOC). The mulch layer comprised a large amount of C (~18 t ha -1 C) especially within humid grasslands. Although grazing reduced C densities in litter mass, total ecosystem C was 8.5 % greater under grazing (127.8 t ha -1 ) compared to those non-grazed (117.8 t ha -1 ), primarily due to increases in SOC and roots. Increases in SOC were consistently observed in the 0-15 cm layer across all climatic conditions, with changes in SOC of the 15-30 cm layer inversely related to aridity. A structural equation model revealed that increased SOC under grazing was indirectly attributed to increases in eudicot rather than graminoid biomass. In addition, SOC increased with graminoid quality (i.e., a reduced carbon to nitrogen ratio), which together with elevated eudicots, increased litter and mulch C, and ultimately enhanced SOC densities. When applied to spatial maps of habitat type and land use (livestock grazing) activity across the region, an area of ~3.8 M ha of grassland was projected to contain an additional 17.1 M t of C under grazing, primarily in mesic grasslands, worth an estimated $3.1 B (Cdn.) under current C valuation guidelines in Canada. Overall, these results highlight the importance of grasslands for C storage and establishing policies that maintain and promote their sustainable use, including light to moderate grazing., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Applying organic amendments to soil can increase soil organic carbon (SOC) storage and reduce greenhouse gas (GHG) emissions generated by agriculture, helping to mitigate climate change. However, it is necessary to determine which type of amendment produces the most desirable results. We conducted a 3-y field study comparing one-time addition of manure compost and its biochar derivative to a control to assess their effects on SOC and GHG emissions at ten annually cropped sites in central Alberta, Canada. Manure compost and biochar were applied at equivalent carbon rates (7 Mg ha -1 ) and tilled into the surface 10 cm of soil. Two years post-treatment, biochar addition increased surface (0-10 cm) SOC by 12 and 10 Mg ha -1 relative to the control and manure addition, respectively. Therefore, biochar addition led to the sequestration of SOC at a rate of 2.5 Mg ha -1 y -1 relative to the control. No treatment effect on deeper (10-100 cm) or cumulative SOC was found. In 2018 and 2019, manure addition increased cumulative GHG (sum of CO 2 , CH 4 , and N 2 O) emissions by 33%, on average, due to greater CO 2 emissions relative to both the control and biochar addition. In contrast, in 2020, biochar addition reduced cumulative GHG emissions by an average of 21% due to lower CO 2 emissions relative to both the control and manure addition. Our study shows that the application of biochar, rather than its manure compost feedstock, increased surface SOC sequestration and had either no effect on (first two years) or reduced GHG emissions (year three) relative to the control. We recommend that policy and carbon sequestration initiatives focus on optimizing biochar production-application systems to fully realize the potential of biochar application as a viable climate change mitigation practice in agriculture., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Adaptive multi-paddock (AMP) grazing, a grazing system in which individual paddocks are grazed for a short duration at a high stock density and followed by a long rest period, is claimed to be an effective tool to sustainably manage and improve grasslands and enhance their ecosystem services. However, whether AMP grazing is superior to conventional grazing (n-AMP) in reducing soil greenhouse gas (GHG) emissions is unclear. Here, we measured CO 2 , CH 4 , and N 2 O fluxes between August 2017 and August 2019 in 12 pairs of AMP vs. n-AMP ranches distributed across an agro-climatic gradient in Alberta, Canada. We found that field GHG fluxes did not differ between AMP and n-AMP grazing systems, but instead were regulated by specific management attributes, environmental conditions, and soil properties, including cattle stocking rate, cultivation history, soil moisture content, and soil bulk density. Specifically, we found that seasonal mean CO 2 emissions increased with increasing cattle stocking rates, while CH 4 uptake was lower in grasslands with a history of cultivation. Seasonal mean CO 2 emissions increased while CH 4 uptake decreased with increasing soil moisture content. In addition, CH 4 uptake decreased with increasing soil bulk density. Observed N 2 O emissions were poorly predicted by the management, environmental conditions, and soil properties investigated in our study. We conclude that AMP grazing does not have an advantage over n-AMP grazing in reducing GHG fluxes from grasslands. Future efforts to develop optimal management strategies (e.g., the use of sustainable stocking rates and avoided cultivation) that reduce GHG emissions should also consider the environmental conditions and soil properties unique to every grassland ecosystem., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Long-term livestock grazing (here after 'grazing') affects carbon (C) and nutrient cycling in grassland ecosystems, in part by altering the quantity and quality of litter inputs. Despite their spatial extent and size of carbon and nutrient stocks, the effect of grazing on grassland biogeochemical cycling through the mediation of microbial activity remains poorly understood. To better understand the relationship between grazing and C and nutrient cycling in litter, we conducted an 18-month long study in paired grasslands previously grazed and nongrazed by cattle for 25 years, measuring extracellular enzyme activity (EEA) in various plant litter samples. Litter sources, including seven grass species dominant in one or more subregions and possessing divergent responses to grazing, as well as a community mix of litter sourced from each site, were tested at 15 sites spanning three grassland subregions in Alberta, Canada. We quantified EEAs associated with C cycling (β-glucosidase, β-Cellobiosidase and β-xylosidase), nitrogen (N) cycling (N-acetyl-glucosaminidase) and phosphorus (P) cycling (phosphatase). In general, litter in grasslands exposed to grazing had greater activity of C-liberating and P-liberating enzyme (β-xylosidase and phosphatase) in the mesic grasslands of the Foothills Fescue subregion (P ≤ 0.10). Observed EEAs were strongly mediated by litter type, with greater EEAs in litter of grass species known to increase in abundance under long-term grazing, including Poa pratensis in the Foothills Fescue subregion, and Bouteloua gracilis in arid grasslands of the Mixedgrass Prairie. In contrast, Pascopyrum smithii litter had the lowest enzyme activities in all subregions. We also found that EEAs changed through time (0-18 months) with consistently high levels detected at 1 (June 2014), 6 (October 2014) and 18 months (October 2015) after placement. Overall, these findings indicate grazing enhances EEA, and thus C and N-cycling, in northern temperate grasslands., (Copyright © 2019 Elsevier B.V. All rights reserved.)

The role of agroforestry systems in mitigating greenhouse gas (GHG) emission from agricultural soils during spring thaw (early April to mid-May) has been poorly studied. Soil CO 2 , CH 4 and N 2 O fluxes were measured from treed areas and adjacent herblands (areas without trees) during spring thaw in 2014 and 2015 at 36 agroforestry sites (12 hedgerow, 12 shelterbelt and 12 silvopasture) in central Alberta, Canada. Fluxes of those GHGs varied with agroforestry systems and land-cover types. We found greater CO 2 emission (P < 0.001) and CH 4 uptake (P < 0.05), but lower N 2 O emission (P < 0.01) in the silvopasture than in the hedgerow and shelterbelt systems, with no difference between the last two systems. Treed areas in general had greater CO 2 emissions (P < 0.001) and CH 4 uptake (P < 0.01), and lower N 2 O emissions (P < 0.001) than the herblands. Soil temperature, moisture content, organic C content and soil available N concentration affected GHG fluxes. The global warming potential (GWP) was greater (P < 0.05) in the silvopasture than in the hedgerow or shelterbelt systems over the two spring thaw seasons examined, and greater (P < 0.05) in the treed areas than in the herblands during the cool spring in 2015. However, the GWP per unit soil organic C was lower in the treed areas (0.004-0.101%) than in the herblands (0.005-0.225%). As compared to previously reported mean growing season GHG emission (15.4 g CO 2 -eq m -2  day -1 ), the GWP of these land uses during spring thaw was small (<5% of the annual GWP) due to the short spring period (6 weeks) and the small GHG emission (2.5 g CO 2 -eq m -2  day -1 ). Although GHG emissions during spring thaw were small compared to those in the growing season, they should not be ignored., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Western Canada's prairie region is extensively cultivated for agricultural production, which is a large source of greenhouse gas emissions. Agroforestry systems are common land uses across Canada, which integrate trees into the agricultural landscape and could play a substantial role in sequestering carbon and mitigating increases in atmospheric GHG concentrations. We measured soil CO2, CH4 and N2O fluxes and the global warming potential of microbe-mediated net greenhouse gas emissions (GWPm) in forest and herbland (areas without trees) soils of three agroforestry systems (hedgerow, shelterbelt and silvopasture) over two growing seasons (May through September in 2013 and 2014). We measured greenhouse gas fluxes and environmental conditions at 36 agroforestry sites (12 sites for each system) located along a south-north oriented soil/climate gradient of increasing moisture availability in central Alberta, Canada. The temperature sensitivity of soil CO2 emissions was greater in herbland (4.4) than in forest (3.1), but was not different among agroforestry systems. Over the two seasons, forest soils had 3.4% greater CO2 emission, 36% higher CH4 uptake, and 66% lower N2O emission than adjacent herbland soils. Combining the CO2 equivalents of soil CH4 and N2O fluxes with the CO2 emitted via heterotrophic (microbial) respiration, forest soils had a smaller GWPm than herbland soils (68 and 89kgCO2ha(-1), respectively). While emissions of total CO2 were silvopasture>hedgerow>shelterbelt, soils under silvopasture had 5% lower heterotrophic respiration, 15% greater CH4 uptake, and 44% lower N2O emission as compared with the other two agroforestry systems. Overall, the GWPm of greenhouse gas emissions was greater in hedgerow (88) and shelterbelt (85) than in the silvopasture system (76kgCO2ha(-1)). High GWPm in the hedgerow and shelterbelt systems reflects the greater contribution from the monoculture annual crops within these systems. Opportunities exist for reducing soil greenhouse gas emissions and mitigating climate change by promoting the establishment of perennial vegetation in the agricultural landscape., (Copyright © 2016 Elsevier B.V. All rights reserved.)