1. Electrochemical biosensor based on Se-doped MWCNTs-graphene and Y-shaped DNA-aided target-triggered amplification strategy.
- Author
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Wang YH, Chen YX, Wu X, and Huang KJ
- Subjects
- Animals, Aptamers, Nucleotide chemistry, Becaplermin blood, Cattle, G-Quadruplexes, Hemin chemistry, Humans, Nanotubes, Carbon ultrastructure, Reproducibility of Results, Biosensing Techniques methods, DNA analysis, Electrochemical Techniques methods, Graphite chemistry, Nanotubes, Carbon chemistry, Selenium chemistry
- Abstract
A highly sensitive electrochemical biosensor for detection of platelet-derived growth factor-BB (PDGF-BB) is developed by using Se-doped multi-walled carbon nanotubes (MWCNTs)-graphene hybrids as electrode supporting substrate, hemin/G-quadruplex as trace labels and Y-shaped DNA-aided target recycling as signal magnifier. The aptamer-containing hairpin probes were first immobilized on the electrode. When target PDGF-BB was added, the aptamer binded PDGF-BB to trigger catalytic assembly of two other hairpins to form many G-quadruplex Y-junction DNA structures, which released PDGF-BB to again bind the intact aptamer to initiate another assembly cycle. G-quadruplex/hemin complexes were produced when hemin was added to generate substantially amplified current output. The developed assay showed a linear range toward PDGF-BB from 0.1 pM to 10 nM with a detection limit of 27 fM (S/N = 3). The method showed excellent specificity and repeatability, and could be expediently applied for sensitive detection of other molecules by simply changing the aptamers., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
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