1. Preparation of ionic liquid modified magnetic metal-organic frameworks composites for the solid-phase extraction of α-chymotrypsin.
- Author
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Wei X, Wang Y, Chen J, Xu P, and Zhou Y
- Subjects
- Animals, Cattle, Chymotrypsin chemistry, Complex Mixtures chemistry, Enzyme Assays, Equipment Reuse, Ferrosoferric Oxide chemistry, Hemoglobins isolation & purification, Magnetite Nanoparticles ultrastructure, Nanotubes, Carbon chemistry, Osmolar Concentration, Ovalbumin isolation & purification, Pancreas chemistry, Sensitivity and Specificity, Serum Albumin, Bovine isolation & purification, Swine, Zeolites chemistry, Chymotrypsin isolation & purification, Ionic Liquids chemistry, Magnetite Nanoparticles chemistry, Metal-Organic Frameworks chemistry, Solid Phase Extraction methods
- Abstract
A novel magnetic solid-phase extraction (MSPE) method based on 1-hexyl-3-methyl imidazolium chloride ionic liquid (IL) modified magnetic Fe
3 O4 nanoparticles, hydroxylated multiwall carbon nanotubes (MWCNTs-OH) and zeolitic imidazolate frameworks (ZIFs) nanocomposites (Fe3 O4 -MWCNTs-OH@ZIF-67@IL) were proposed and applied to extract α-chymotrypsin. The magnetic materials were synthesized successfully and characterized by X-ray diffraction (XRD), transmission electron microscope (TEM), thermal gravimetric analysis (TGA), fourier transform infrared spectrometry (FT-IR), vibrating sample magnetometer (VSM) and zeta potentials. Subsequently, the UV-vis spectrophotometer at about 280 nm was utilized to quantitatively analyze the α-chymotrypsin concentration in the supernatant. Furthermore, single factor experiments revealed that the extraction capacity was influenced by initial α-chymotrypsin concentration, ionic strength, extraction time, extraction temperature and pH value. The extraction capacity could reach up to about 635 mg g-1 under the optimized conditions, absolutely higher than that of extraction for Ovalbumin (OVA), Bovine serum albumin (BSA) and Bovine hemoglobin (BHb). In addition, the regeneration studies showed Fe3 O4 -MWCNTs-OH@ZIF-67@IL particles could be reused several times and kept a high extraction capacity. Besides, the study of enzymatic activity also indicated that the activity of the extracted α-chymotrypsin was well maintained 93% of initial activity. What's more, the proposed method was successfully applied to extract α-chymotrypsin in porcine pancreas crude extract with satisfactory results. All of above conclusions highlight the great potential of the proposed Fe3 O4 -MWCNTs-OH@ZIF-67@IL-MSPE method in the analysis of biomolecules., (Copyright © 2018 Elsevier B.V. All rights reserved.) more...- Published
- 2018
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