Search

Your search keyword '"Czerwińska, Monika E."' showing total 3 results
Start Over Author "Czerwińska, Monika E." Publisher elsevier
3 results on '"Czerwińska, Monika E."'

1. Symphytum officinale L. : liquid-liquid chromatography isolation of caffeic acid oligomers and evaluation of their influence on pro-inflammatory cytokine release in LPS-stimulated neutrophils

Author

Trifan, Adriana, Skalicka-Woźniak, Krystyna, Granica, Sebastian, Czerwińska, Monika E., Kruk, Aleksandra, Marcourt, Laurence, Wolfender, Jean-Luc, Wolfram, Evelyn, Esslinger, Nils, Grubelnik, Andreas, Luca, Simon Vlad, Trifan, Adriana, Skalicka-Woźniak, Krystyna, Granica, Sebastian, Czerwińska, Monika E., Kruk, Aleksandra, Marcourt, Laurence, Wolfender, Jean-Luc, Wolfram, Evelyn, Esslinger, Nils, Grubelnik, Andreas, and Luca, Simon Vlad

Abstract

Ethnopharmacological relevance: Symphytum officinale L. (comfrey, Boraginaceae) has been traditionally used for millennia in joint distortions, myalgia, bone fractures and hematomas. However, key activity-determining constituents and molecular mechanisms underlying its use have not been completely elucidated. Aim of the study: The objective of this study was to isolate and identify the major compounds from a hydro-ethanolic root extract of S. officinale and evaluate their antioxidant potential, alongside their effect on the cytokine production of ex vivo stimulated neutrophils, thus providing scientific support for the traditional use of comfrey root. Material and methods: Four caffeic acid oligomers were isolated from comfrey roots by liquid-liquid chroma-tography, their structures being established by MS and NMR analyses. In vitro antioxidant evaluation was per-formed by DPPH and ABTS assays. The cytotoxicity of isolated compounds was established by flow cytometry. The effect on cytokine release, such as interleukin (IL)-1β, IL-8 and tumor necrosis factor alpha (TNF-α), in lipopolysaccharide (LPS)-stimulated neutrophils was determined by enzyme-linked immunosorbent assay (ELISA). Results: The main constituents found in comfrey root were represented by four caffeic acid oligomers, namely globoidnan B (1), rabdosiin (2), rosmarinic acid (3) and globoidnan A (4). Rabdosiin, globoidnans A and B were isolated for the first time from S. officinale. In the in vitro antioxidant tests, compound 2 was the most active, with EC50 values in DPPH and ABTS assays of 29.14 ±0.43 and 11.13 ±0.39, respectively. Neutrophils’ viability over the tested concentration domain of 12.5–50 μM was not altered. At 50 μM, all compounds significantly inhibited IL-1β release, with compound 3 (45.60% release vs. LPS stimulated neutrophils) being the most active, followed by compounds 1 (53.85%), 2 (69.89%) and 4 (60.68%). Conclusions: The four caffeic acid oligomers reported in S. officinale root

Published
2020

2. The influence of procyanidins isolated from small-leaved lime flowers (Tilia cordata Mill.) on human neutrophils.

Author

Czerwińska ME, Dudek MK, Pawłowska KA, Pruś A, Ziaja M, and Granica S

Subjects
Anti-Inflammatory Agents isolation & purification, Apoptosis, Biflavonoids isolation & purification, Catechin isolation & purification, Cells, Cultured, Cytokines metabolism, Humans, N-Formylmethionine Leucyl-Phenylalanine, Proanthocyanidins isolation & purification, Reactive Oxygen Species metabolism, Anti-Inflammatory Agents pharmacology, Biflavonoids pharmacology, Catechin pharmacology, Flowers chemistry, Neutrophils drug effects, Proanthocyanidins pharmacology, Tilia chemistry
Abstract

Linden flower is a wildly used plant material among patients in the treatment of common cold symptoms and mucosa inflammations. However, the structure and bioactivity of flavan-3-ol derivatives present in infusions from flowers of Tilia cordata have not been studied so far. The aim of current study was to isolate and identify main procyanidins present in the flowers of small-leaved lime and to evaluate their influence on the inflammatory response of human neutrophils ex vivo. The chemical structure of isolated compounds was established by 1D and 2D NMR experiments. The bioactivity of obtained compounds was tested in human neutrophils model. Cytotoxicity and influence of compounds on apoptosis was established by flow cytometry. The levels of produced cytokines were established by ELISA after stimulation with lipopolysaccharide (LPS). The inhibition of the production of reactive oxygen species was checked by luminol-dependent chemiluminescence method after N-formylmethionyl-leucyl-phenylalanine (f-MLP) induction. The phytochemical work resulted in the isolation of 10 compounds. Compounds were identified as oligomeric procyanidins and their precursor epicatechin. The potential anti-inflammatory activity of compounds was evaluated in the concentration range 5-20 μM. All compounds were able to decrease the production of ROS from f-MLP-stimulated neutrophils. Most of compounds were able to inhibit the LPS-induced release of IL-8. Some trimeric and tetrameric derivatives were also able to decrease the production of MIP-1β. Obtained results partially support the traditional usage of infusion from lime flowers in the treatment of symptoms of inflammation and irritation of mucosa in common cold, pharyngitis and tonsillitis., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Published
2018
Full Text
View/download PDF

3. Antioxidant and anti-inflammatory flavonol glucuronides from Polygonum aviculare L.

Author

Granica S, Czerwińska ME, Żyżyńska-Granica B, and Kiss AK

Subjects
Anti-Inflammatory Agents chemistry, Anti-Inflammatory Agents isolation & purification, Antioxidants chemistry, Antioxidants isolation & purification, Flavonols chemistry, Flavonols isolation & purification, Glucuronides chemistry, Glucuronides isolation & purification, Humans, Kaempferols chemistry, Kaempferols isolation & purification, Kaempferols pharmacology, Molecular Structure, Neutrophils metabolism, Plant Extracts chemistry, Plant Extracts pharmacology, Quercetin analogs & derivatives, Quercetin chemistry, Quercetin isolation & purification, Quercetin pharmacology, Anti-Inflammatory Agents pharmacology, Antioxidants pharmacology, Flavonols pharmacology, Glucuronides pharmacology, Pancreatic Elastase metabolism, Polygonum chemistry, Reactive Oxygen Species metabolism
Abstract

A series of 11 flavonol glucuronides were isolated from the herb of Polygonum aviculare L. (Ph.Eur) of which 8 were reported for the first time from the Polygonum species. Three acetylated kaempferol and isorhamnetin glucuronides were isolated from a natural source for the first time. All compounds, including the new ones, were characterized using 1D and 2D NMR spectra as well as high resolution mass spectrometry. The influence of all isolated compounds on the production of reactive oxygen species, as well as on elastase release by human neutrophils, was evaluated in in vitro studies. The results showed that all investigated compounds at physiologically achievable concentrations within the range of 0.5-10 μM significantly inhibited the production of reactive oxygen species as well as elastase release in human neutrophils model and should be considered as responsible for anti-inflammatory activity of the P. aviculare herb. The chemotaxonomic value of isolated compounds was also discussed., (© 2013.)

Published
2013
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results