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Understanding the relationship between heat wave occurrence and wildfire spread represents a key priority in global change studies due to the significant threats posed on natural ecosystems and society. Previous studies have not explored the spatial and temporal mechanism underlying the relationship between heat waves and wildfires occurrence, especially over large geographical regions. This study seeks to investigate such a relationship with a focus on 37 ecoregions within a Eurasia longitudinal gradient. The analysis is based on the wildfire dataset provided by the GlobFire Final Fire Event Detection and the meteorological dataset ERA5-land from Copernicus Climate service. In both cases we focused on the 2001-2019 timeframe. By means of a 12 km square grid, three wildfire metrics, i.e., density, seasonality, and severity of wildfires, were computed as proxy of fire regime. Heat waves were also characterized in terms of periods, duration, and intensity for the same period. Statistical tests were performed to evaluate the different patterns of heat wave and wildfire occurrence in the 37 ecoregions within the study area. By using Geographically Weighted Regression (GWR) we modeled the spatial varying relationships between heat wave characteristics and wildfire metrics. As expected, our results suggest that the 37 ecoregions identified within the Eurasia longitudinal gradient differ in terms of fire regimes. However, the occurrence of heat waves did not show significant differences among ecoregions, but a more evident variability in terms of relationship between fire regime metrics and heat waves within the study area. The outcome of the GWR analysis allowed us to identify the spatial locations (i.e., hotspot areas) where the relationship between heat waves and wildfires is positive and significant. Hence, in hotspots the presence of heat waves can be seen as a driver of wildfire occurrence in forest and steppe ecosystems. The findings from this study could contribute to a more comprehensive assessment of wildfire patterns in this geographical region, thus supporting cross-regional prevention strategies for disaster risk mitigation., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

Plants produce a plethora of volatile organic compounds (VOCs) which are important in determining the quality and nutraceutical properties of horticultural food products, including the taste and aroma of wine. Given that some of the most prevalent grape aroma constituents are terpenoids, we investigated the possible variations in the relative expression of terpene synthase (TPS) genes that depend on the organ. We thus analysed mature leaves, young leaves, stems, young stems, roots, rachis, tendrils, peduncles, bud flowers, flowers and berries of cv Moscato bianco in terms of their VOC content and the expression of 23 TPS genes. In terms of the volatile characterization of the organs by SPME/GC-MS analysis, flower buds and open flowers appeared to be clearly distinct from all the other organs analysed in terms of their high VOC concentration. Qualitatively detected VOCs clearly separated all the vegetative organs from flowers and berries, then the roots and rachis from other vegetative organs and flowers from berries, which confirms the specialization in volatile production among different organs. Our real-time RT-PCR results revealed that the majority of TPS genes analysed exhibited detectable transcripts in all the organs investigated, while only some were found to be expressed specifically in one or just a few organs. In most cases, we found that the known products of the in vitro assay of VvTPS enzymes corresponded well to the terpenes found in the organs in which the encoding gene was expressed, as in the case of (E)-β-caryophyllene synthases, α-terpineol synthase and α-farnesene synthase. In addition, we found groups of homologous TPS genes, such as (E)-β-caryophyllene and β-ocimene synthases, expressed distinctively in the various tissues. This thus confirmed the subfunctionalization events and a specialization on the basis of the organs in which they are mostly expressed., (Copyright © 2014 Elsevier Ltd. All rights reserved.)

Retroviruses of the HTLV and HIV subfamilies share a number of similar properties in terms of route of transmission and ultimate targeting to CD4+ T-cells, which results in leukaemic transformation in the case of HTLV and in depletion of CD4+ population and failure of T-helper function in HIV-infected individuals. Both diseases gain poor benefit from therapy at the stage of clinical diagnosis as ATL leukaemia or AIDS, respectively. Therefore the best chances to limit the worldwide distribution of these human retroviruses reside in an effective prevention of viral diffusion, possibly by vaccination, and of the onset of disease in virus-positive subjects. The possibility is discussed of defining protocols to prevent the development of clinical disease. These protocols could be based on pharmacological reconstitution of host's immune reactivity, that is altered early after infection with these retroviruses, and on the control of virus replication by antiviral therapy.

Human mononuclear cells derived from peripheral blood of adult donors (PBMC) or from neonatal cord blood (CBMC) were found to be equally sensitive to the protective effect of alpha- and beta-interferons (IFNs) against the infection with HTLV-I during long-term culture. The effect of IFNs was evidenced by a remarkable reduction of the number of virus-positive cells during culture as evaluated by indirect immunofluorescence for the p19 virus core protein. Moreover, the appearance of p19-positive immortalized clones was inhibited by IFNs in PBMC co-cultures, whereas it was delayed in CBMC cultures. These kinetics are in relation with the higher permissivity of CBMC to the virus in comparison with PBMC, since in CBMC cultures infected cells can be clearly detected starting already 1 week post-infection (p.i.), whereas in PBMC cultures their appearance time is approximately at the 6th week p.i. IFNs acted by 'priming' PBMC and CBMC to an active antiviral competence, since one single treatment with 1000 IU/ml of alpha- or beta-IFN at the onset of the co-culture of mononuclear cells with irradiated virus-donor cells was able to maintain very low levels of infection for approximately 6 weeks in CBMC cultures and at least for 18 weeks in PBMC cultures. As a consequence, it seems likely that IFN action is mediated by the expression of a defined, although not completely identified, set of genes in the host cells.

In vitro infection of human cord blood lymphocytes (CBL) with human T-cell leukemia/lymphoma virus type I (HTLV-I) was found to be reduced by suramin treatment at a concentration ranging from 10-100 micrograms/ml. At higher concentrations (500 micrograms/ml) suramin was toxic to the cells and even resulted in an increased percentage of cells positive for the p19 viral core protein. Suramin treatment at the onset of the CBL coculture with a lethally irradiated HTLV-I donor cell line (MT-2) reduced virus transmission, evaluated as number of p19+ cells, and the consequent amount of integrated provirus in the host genome. The amount of viral RNA transcripts was not reduced in CBL cocultures. On the other hand, suramin affected HTLV-I replication in infected MT-2 cells, when used at a concentration of 50 micrograms/ml, and this might contribute to the reduced infectivity of suramin-treated MT-2 cells. In addition to its antiviral effects, suramin exerted a modest positive regulation on the natural killing activity of CBL and their early proliferative response in mixed lymphocyte/tumor cell culture.

A method is described which allows human peripheral blood monocytes from any given donor to differentiate in vitro into mature macrophages. About 90% of the starting monocytes are maintained during the long-term culture and are matured to macrophages. Thus cell loss is minimal and the resulting population of mature macrophages can be regarded as representative for all possible macrophage subpopulations present in peripheral blood. These cultures represent a standardized model for in-vitro studies on the role of mature macrophages in various immunological reactions.

Peripheral mononuclear cells (MNC) collected from 12 healthy donors and 44 leukemic patients at various stages of the disease were tested for natural killer (NK) activity and for their susceptibility to HTLV-I infection in vitro, measured in terms of percentage of p19 positive cells. MNC from leukemic donors at any stage of leukemia (ie, onset or relapse, ON/REL; complete remission or off-therapy, CR/OT donors) were highly susceptible to HTLV-I infection. This was true for acute leukemias of lymphoblastic (ALL) or nonlymphoblastic (ANLL) type. MNC of ON/REL patients were more susceptible to HTLV-I than those of CR/OT donors. In addition, leukemic blasts were more rapidly infected (ie, within five to seven days) than the HTLV-I-susceptible normal cord-blood lymphocytes. However, the presence of circulating blasts was not essential to virus susceptibility, since CR/OT MNC, presumably free of leukemic blasts, were still more susceptible to HTLV-I than normal cells. Basal NK function of MNC from leukemic patients was significantly lower than that detectable in healthy controls. However, no correlation was found between susceptibility to HTLV-I infection and NK activity.

The in vitro differentiation and maturation of resident and activated mouse and human macrophages (M phi) from different anatomical sources was investigated with regard to their oxygen metabolism during zymosan phagocytosis. We found evidence that chemiluminescence (CL) of M phi depends upon their differentiation stage: a) In the absence of any phagocytic stimulus, the human M phi showed a lucigenin-dependent CL background that was approximately 10-fold higher than in mouse M phi and decreased to low levels in resident M phi (monocyte-derived human M phi). This background was reduced by SOD to about 50%. No relevant luminol-dependent background was observed in all mouse and human M phi during culture time. b) Resident and activated mouse and human M phi could be distinguished in terms of their lucigenin-dependent CL during zymosan phagocytosis, which was persistently high in activated M phi, but decreased to comparatively low levels in resident M phi during culture time. This zymosan-elicited CL was almost completely SOD-dependent during all culture time. c) A dissociation between phagocytosis and oxygen radical release is observed: the decrease of both minolul and lucigenin-dependent CL in resident phagocytizing M phi during maturation did not correspond to a decrease of their phagocytic activity. Phagocytosis occurred at a high rate also in the absence of a relevant CL-detectable generation of oxygen radicals. The oxygen radical release, as measured by SOD-inhibitable cytochrome c reduction, paralleled CL during zymosan phagocytosis and declined with maturation of monocytes into M phi. In contrast, the zymosan-induced nitro-blue-tetrazolium reduction increased in mature resident human M phi. Thus, it seems that different metabolic pathways are utilized during phagocytosis in young and mature M phi.