1. FasL-induced apoptosis in bovine oocytes via the Bax signal.
- Author
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Li HJ, Wang CY, Mi Y, Du CG, Cao GF, Sun XC, Liu DJ, and Shorgan B
- Subjects
- Animals, Annexin A5, Apoptosis, Cumulus Cells metabolism, Fas Ligand Protein genetics, Female, Gene Expression Regulation, In Situ Nick-End Labeling, Protein Transport, Proto-Oncogene Proteins c-bcl-2 genetics, Proto-Oncogene Proteins c-bcl-2 metabolism, RNA, Messenger genetics, RNA, Messenger metabolism, Signal Transduction physiology, Staining and Labeling, bcl-2-Associated X Protein genetics, Cattle, Fas Ligand Protein metabolism, Oocytes physiology, bcl-2-Associated X Protein metabolism
- Abstract
The factor associated suicide (Fas) and its ligand (FasL) signaling is an important regulatory pathway of apoptosis in mammalian follicles. However, whether apoptosis in bovine oocytes is regulated by the Fas-FasL signaling pathway remains unknown. In this study, localization of Fas and FasL in immature oocytes and FasL in cumulus cells were examined using immunofluorescence staining. In addition, exogenous FasL was added to an in vitro culture system to investigate apoptotic changes in bovine oocytes, using annexin-V and terminal uridine nick-end labeling staining, and real-time quantitative polymerase chain reaction. In this study, Fas was expressed in immature oocytes, whereas FasL was expressed in cumulus cells, but not in immature oocytes; annexin-V- and terminal uridine nick-end labeling-positive rates of oocytes treated with 2, 10, or 50 ng/mL FasL were higher than those of control oocytes (P < 0.05); and oocytes from the three treatment groups had higher expression levels of Fas and B cell lymphoma/leukemia-2 associated X than those in the control group (P < 0.05). Taken together, we concluded that the Fas-FasL signaling pathway was involved in regulation of bovine oocyte apoptosis, perhaps related to B cell lymphoma/leukemia-2 associated X upregulation., (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Published
- 2013
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