1. Protocol for optimizing production and quality control of infective EcoHIV virions
- Author
-
Hammodah R. Alfar, Daphne N. Pariser, Harry Chanzu, Smita Joshi, Daniëlle M. Coenen, Joshua Lykins, Kanakanagavalli Shravani Prakhya, Mary Jane Potash, Wei Chao, Jennifer Kelschenbach, David J. Volsky, Kelly Metcalf-Pate, and Sidney W. Whiteheart
- Subjects
Cell Culture ,Cell-based Assays ,Health Sciences ,Science (General) ,Q1-390 - Abstract
Summary: EcoHIV is a model of HIV infection that recapitulates aspects of HIV-1 pathology in mice. However, there are limited published protocols to guide EcoHIV virion production. Here, we present a protocol for producing infective EcoHIV virions and essential quality controls. We describe steps for viral purification, titering, and multiple techniques to analyze infection efficacy. This protocol produces high infectivity in C57BL/6 mice which will aid investigators in generating preclinical data. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.
- Published
- 2023
- Full Text
- View/download PDF