30 results on '"De Felice, M."'
Search Results
2. Regulation of Isoleucine and Valine Biosynthesis
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Maurizio Iaccarino, John Guardiola, Renée Favre, and De Felice M
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chemistry.chemical_classification ,Acetolactate synthase ,Acetolactate synthase activity ,Transamination ,Stereochemistry ,Threonine Dehydratase ,Biology ,chemistry ,Biochemistry ,Valine ,biology.protein ,Isoleucine ,Threonine ,Amino acid synthesis - Abstract
Publisher Summary This chapter discusses the regulation of isoleucine and valine biosynthesis. Isoleucine and valine biosynthesis is a model system for the study of mechanisms by which the cell coordinates different parts of its total metabolism. Threonine deaminase is the first enzyme in isoleucine biosynthesis. A different threonine deaminase, termed catabolic, appears only in anaerobiosis and in the absence of glucose. A third threonine deaminase, present in Escherichia coli K-12 and Salmonella Typhimurium, deaminates only D-threonine, and its presence explains why ilvA mutants of these organisms grow upon addition of D-threonine to the medium. The second step in the pathway is common to isoleucine and valine biosynthesis and is catalyzed by acetolactate synthase activity. It involves the conversion of either two molecules of pyruvate to form α-acetolactate, or one molecule of pyruvate and one molecule of α-ketobutyrate to form α-aceto-α-hydroxybutyrate. The acetolactate synthase isoenzymes have some relation to lysine biosynthesis. The third step in isoleucine biosynthesis and the second in valine biosynthesis are catalyzed by the enzyme isomeroreductase. The last intermediates in the pathway for isoleucine and valine biosynthesis are the keto acids from which the corresponding amino acids are synthesized by transamination.
- Published
- 1978
3. Advancing climate services for the European renewable energy sector through capacity building and user engagement
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Lucien Wald, Laurent Dubus, Barbara Percy, Claire Thomas, Clare Goodess, Alberto Troccoli, Philip E. Bett, Stephen Dorling, Mélodie Trolliet, David Brayshaw, Thierry Ranchin, C. Acton, M. De Felice, L. Penny, Juan A. Añel, School of Environmental Sciences, University of East Anglia, University of East Anglia, World Energy & Meteorology Council, UK Met Office, Environmental Physics Laboratory (EPhysLab), Universidade de Vigo, Department of Meteorology, University of Reading, Reading, UK, National Centre for Atmospheric Science, University of Reading, Reading, United Kingdom, ENEA Climate Modelling Laboratory, EDF R&D (EDF R&D), EDF (EDF), Institute for Environmental Analytics, Centre Observation, Impacts, Énergie (O.I.E.), MINES ParisTech - École nationale supérieure des mines de Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL), Transvalor, Transvalor S.A., Goodess, C. M., Troccoli, A., Acton, C., Anel, J. A., Bett, P. E., Brayshaw, D. J., De Felice, M., Dorling, S. R., Dubus, L., Penny, L., Percy, B., Ranchin, T., Thomas, C., Trolliet, M., and Wald, L.
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Renewable energy ,Atmospheric Science ,Process management ,010504 meteorology & atmospheric sciences ,Download ,020209 energy ,Target audience ,3308 Ingeniería y Tecnología del Medio Ambiente ,02 engineering and technology ,Technology readiness level ,lcsh:QC851-999 ,01 natural sciences ,7. Clean energy ,Stakeholder engagement ,[SPI]Engineering Sciences [physics] ,Documentation ,Community of practice ,User needs and requirements ,Climate service ,Climate services ,Co-production ,Sectoral information system ,0202 electrical engineering, electronic engineering, information engineering ,lcsh:Social sciences (General) ,0105 earth and related environmental sciences ,Downstream (petroleum industry) ,Service (business) ,Global and Planetary Change ,business.industry ,Capacity building ,[SDE.PLAN]Environmental Sciences/domain_sde.plan ,2502 Climatología ,3322.05 Fuentes no Convencionales de Energía ,[SDU.STU.CL]Sciences of the Universe [physics]/Earth Sciences/Climatology ,13. Climate action ,[SDE]Environmental Sciences ,lcsh:Meteorology. Climatology ,lcsh:H1-99 ,Business - Abstract
The development of successful climate services faces a number of challenges, including the identification of the target audience and their needs and requirements, and the effective communication of complex climate information, through engagement with a range of stakeholders. This paper describes how these challenges were tackled during the European Climatic Energy Mixes (ECEM) project, part of the Copernicus Climate Change Service (C3S), in order to deliver a pre-operational, proof-of-concept climate service for the European renewable energy sector. The process of iterative user engagement adopted in ECEM is described, from the initial presentation of the team’s first vision for such a service to support external stakeholders, through to evaluation of the final interactive tool for visualisation, data download and supporting documentation (the C3S ECEM Demonstrator). The outcomes of this evaluation are outlined, together with a retrospective reflection on the engagement and development process. The extent to which co-production and co-design were achieved in practice is assessed. The paper also highlights the distance travelled from the start to end of ECEM in terms of building capacity, developing a community of practice, and raising the Technology Readiness Level. The relevance of ECEM for the European climate services market is briefly considered, including the development of downstream commercial services which build upon the public C3S services. Keywords: Climate services, Sectoral information system, Renewable energy, User needs and requirements, Stakeholder engagement, Co-production
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- 2019
4. Scoping the potential usefulness of seasonal climate forecasts for solar power management
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Andrea Alessandri, Alberto Troccoli, Matteo De Felice, Marta Bruno Soares, De Felice, M., Soares, M. B., Alessandri, A., and Troccoli, A.
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bepress|Physical Sciences and Mathematics ,Climate ,Climate services ,Forecasting ,Solar power ,Computer science ,EarthArXiv|Physical Sciences and Mathematics|Environmental Sciences ,media_common.quotation_subject ,020209 energy ,bepress|Physical Sciences and Mathematics|Earth Sciences ,02 engineering and technology ,EarthArXiv|Physical Sciences and Mathematics|Earth Sciences ,7. Clean energy ,Climate service ,0202 electrical engineering, electronic engineering, information engineering ,Production (economics) ,0601 history and archaeology ,Quality (business) ,bepress|Physical Sciences and Mathematics|Environmental Sciences ,bepress|Physical Sciences and Mathematics|Environmental Sciences|Sustainability ,media_common ,060102 archaeology ,EarthArXiv|Physical Sciences and Mathematics|Environmental Sciences|Sustainability ,Renewable Energy, Sustainability and the Environment ,business.industry ,Photovoltaic system ,06 humanities and the arts ,Environmental economics ,Solar energy ,021001 nanoscience & nanotechnology ,Renewable energy ,EarthArXiv|Physical Sciences and Mathematics ,Nameplate capacity ,13. Climate action ,Electricity ,business ,0210 nano-technology - Abstract
Solar photovoltaic energy is widespread worldwide and particularly in Europe, which became in 2016 the first region in the world to pass the 100 GW of installed capacity. As with all the renewable energy sources, for an effective management of solar power, it is essential to have reliable and accurate information about weather/climate conditions that affect the production of electricity. Operations in the solar energy industry are normally based on daily (or intra-daily) forecasts. Nevertheless, information about the incoming months can be relevant to support and inform operational and maintenance activities. This paper discusses a methodology to assess whether a seasonal climate forecast can provide a useful prediction for a specific sector, in this paper the European solar power industry. After evaluating the quality of the forecasts in providing probabilistic information for solar radiation, we describe how to assess their potential usefulness for a generic user by proposing an approach that takes into account not only their accuracy but also other potentially relevant factors. This approach is called index of opportunity and is then illustrated by presenting an example for the European solar power sector. The index of opportunity provides indications about where and when seasonal climate forecasts can benefit the decision-making in the photovoltaic sector. Even more importantly, it suggests an approach on how to evaluate their usefulness for the user's decision-making. This approach has the advantage of not limiting the definition of the usefulness only to the quality of the forecasts but rather considering, in an explicit way, all the factors that must be combined with the forecast's quality to define what is useful or not for the user.
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- 2019
5. A simplified seasonal forecasting strategy, applied to wind and solar power in Europe.
- Author
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Bett PE, Thornton HE, Troccoli A, De Felice M, Suckling E, Dubus L, Saint-Drenan YM, and Brayshaw DJ
- Abstract
We demonstrate levels of skill for forecasts of seasonal-mean wind speed and solar irradiance in Europe, using seasonal forecast systems available from the Copernicus Climate Change Service (C3S). While skill is patchy, there is potential for the development of climate services for the energy sector. Following previous studies, we show that, where there is skill, a simple linear regression-based method using the hindcast and forecast ensemble means provides a straightforward approach for producing calibrated probabilistic seasonal forecasts. This method extends naturally to using a larger-scale feature of the climate, such as the North Atlantic Oscillation, as the climate model predictor, and we show that this provides opportunities to improve the skill in some cases. We further demonstrate that, on seasonal-average and regional (e.g. national) average scales, wind and solar power generation are highly correlated with single climate variables (wind speed and irradiance). The detailed non-linear transformations from meteorological quantities to energy quantities, which are essential for detailed simulation of power system operations, are usually not necessary when forecasting gross wind or solar generation potential at seasonal-mean regional-mean scales. Together, our results demonstrate that where there is skill in seasonal forecasts of wind speed and irradiance, or a correlated larger-scale climate predictor, skilful forecasts of seasonal mean wind and solar power generation can be made based on the climate variable alone, without requiring complex transformations. This greatly simplifies the process of developing a useful seasonal climate service., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Crown Copyright © 2022 Published by Elsevier B.V.)
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- 2022
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6. BAZ1B is a candidate gene responsible for hypothyroidism in Williams syndrome.
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Allegri L, Baldan F, Mio C, De Felice M, Amendola E, and Damante G
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- Cell Line, Down-Regulation, Humans, Transcription Factors metabolism, Hypothyroidism genetics, Thyroid Epithelial Cells metabolism, Transcription Factors genetics, Williams Syndrome genetics
- Abstract
Williams syndrome (WS) is a rare neurodevelopmental disorder associated to a hemizygous deletion of 28 genes located on chromosome 7q11.23. WS affected subjects frequently suffer from several endocrine abnormalities including hypothyroidism due to defects in thyroid morphology. To date, several genes involved in thyroid dysgenesis have been identified, nonetheless, none of them is located in the 7q11.23 region. Thus, the hypothyroidism-linked molecular features in WS are not yet known. In this study we focused on one of the WS deleted gene, BAZ1B, demonstrating that its downregulation in thyroid cells leads to cell viability and survival decrement. Taking together, our results show that BAZ1B could be the mainly responsible for thyroid defects observed in some of WS patients and that these alterations are activated by PTEN-mediated mechanisms., Competing Interests: Declaration of competing interest The authors declare no competing financial interests in relation to this work., (Copyright © 2020 Elsevier Masson SAS. All rights reserved.)
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- 2020
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7. Factors related to the expression of emotions by early-stage breast cancer patients.
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Del Piccolo L, Mazzi MA, Mascanzoni A, Lonardi M, De Felice M, Danzi OP, Buizza C, Ghilardi A, Bottacini A, and Goss C
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- Adolescent, Adult, Aged, Breast Neoplasms pathology, Female, Humans, Italy, Middle Aged, Neoplasm Staging, Breast Neoplasms psychology, Emotions, Physician-Patient Relations
- Abstract
Objective: (1) To report the number and type of emotional expressions (cues/concerns) raised by breast cancer patients; (2) to identify the influence of setting, patient characteristics, and doctor-patient interaction on emotional expression., Methods: 308 Italian-speaking female patients were recruited at their first breast cancer consultation. The visits were audio-recorded and analysed for number and type of emotional expressions (VR-CoDES). Oncologists' interaction skills were rated by the VR-COPE. Socio-demographic, clinical and personality variables were gathered before the consultation. Clinical variables and oncologists' evaluations of the patient were collected after., Results: Breast cancer patients raised emotional issues mainly as cues. The setting (centre where the consultations took place), the oncologist's attribution of anxiety, regardless of anxiety test screening (STAI-X1) score before the consultation, and the oncologist's ability to pick up on patient's worries, handle emotional needs or understand psychosocial condition were all positively related with the number of emotional expressions. More tightly structured consultations had fewer emotional expressions., Conclusion: Both contextual and interactional aspects have an impact on patient emotional expressions., Practice Implications: Oncologists need to be trained to manage both the content and the process of medical consultation. Work organization of the consultation setting needs to be taken into account., (Copyright © 2019 Elsevier B.V. All rights reserved.)
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- 2019
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8. Bisphenol A interferes with thyroid specific gene expression.
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Gentilcore D, Porreca I, Rizzo F, Ganbaatar E, Carchia E, Mallardo M, De Felice M, and Ambrosino C
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- Animals, Benzhydryl Compounds administration & dosage, Dose-Response Relationship, Drug, Endocrine Disruptors administration & dosage, Environmental Pollutants administration & dosage, Environmental Pollutants toxicity, Phenols administration & dosage, Pituitary Gland metabolism, Rats, Thyroid Gland cytology, Time Factors, Zebrafish, Benzhydryl Compounds toxicity, Endocrine Disruptors toxicity, Gene Expression Regulation drug effects, Phenols toxicity, Thyroid Gland drug effects, Thyroid Hormones genetics
- Abstract
Bisphenol A (BPA) is an endocrine-disrupting chemical that leads to low-dose human exposure due to its ability to leach from chemically derived products, as polycarbonate plastics and epoxy resin. In addition to its known xeno-endocrine action, BPA exerts a wide range of metabolic effects. Despite the documented BPA exposure outcomes on synthesis of thyroid hormones, there are not any data available on its actions on the thyroid follicular cells, site of synthesis of the thyroid hormones. Recently, it has been shown that several environmental pollutants, as BPA, can exert a thyroid disrupting activity. In this study, we employed in vitro and in vivo (zebrafish) models to examine the effects of BPA in regulating the expression of genes involved in the thyroid hormone synthesis and of their transcriptional regulators at BPA doses as low as 10(-9)M, a dose that is environmentally pertinent and far below the one detected in infants plasma. In both systems we could detect an altered expression of the genes involved in thyroid hormones synthesis and of thyroid specific transcriptional factors in BPA dose and time dependent manner. Our results suggest that BPA exerts a direct effect on thyroid follicular cell. We show that these cells can "sense" very low amount of BPA. Thus they, potentially, represent an ideal in vitro system to develop assays to detect BPA and other pollutants with thyroid disrupting activity at level far below the ones considered to be environmental relevant. Moreover, this report may provide new insight into the mode of BPA-induced deregulation of physiological processes as well as on the extensively debated molecular pathways underlying its biological activities., (Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.)
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- 2013
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9. Gene expression profiling at early organogenesis reveals both common and diverse mechanisms in foregut patterning.
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Fagman H, Amendola E, Parrillo L, Zoppoli P, Marotta P, Scarfò M, De Luca P, de Carvalho DP, Ceccarelli M, De Felice M, and Di Lauro R
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- Animals, Body Patterning genetics, Digestive System embryology, Digestive System metabolism, Embryo, Mammalian embryology, Female, Gene Expression Profiling, Gene Expression Regulation, Developmental, In Situ Hybridization, Laser Capture Microdissection, Lung embryology, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Oligonucleotide Array Sequence Analysis, Organogenesis genetics, Thyroid Gland embryology, Time Factors, Embryo, Mammalian metabolism, Lung metabolism, Thyroid Gland metabolism, Transcriptome
- Abstract
The thyroid and lungs originate as neighboring bud shaped outgrowths from the midline of the embryonic foregut. When and how organ specific programs regulate development into structures of distinct shapes, positions and functions is incompletely understood. To characterize, at least in part, the genetic basis of these events, we have employed laser capture microdissection and microarray analysis to define gene expression in the mouse thyroid and lung primordia at E10.5. By comparing the transcriptome of each bud to that of the whole embryo as well as to each other, we broadly describe the genes that are preferentially expressed in each developing organ as well as those with an enriched expression common to both. The results thus obtained provide a valuable resource for further analysis of genes previously unrecognized to participate in thyroid and lung morphogenesis and to discover organ specific as well as common developmental mechanisms. As an initial step in this direction we describe a regulatory pathway involving the anti-apoptotic gene Bcl2 that controls cell survival in early thyroid development., (Copyright © 2011 Elsevier Inc. All rights reserved.)
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- 2011
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10. The 4th European Spores Conference, Cortona (Italy), May 27-29, 2010.
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De Felice M, Ricca E, Fouet A, Barák I, and Cutting S
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- Bacteria cytology, Bacteria growth & development, Bacteria metabolism, Cell Division, Signal Transduction, Spores, Bacterial cytology, Spores, Bacterial growth & development, Spores, Bacterial metabolism
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- 2010
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11. Characterization of intestinal bacteria tightly bound to the human ileal epithelium.
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Fakhry S, Manzo N, D'Apuzzo E, Pietrini L, Sorrentini I, Ricca E, De Felice M, and Baccigalupi L
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- Bacteria growth & development, Bacteria metabolism, Bifidobacterium growth & development, Bifidobacterium isolation & purification, Bifidobacterium metabolism, Humans, Lactobacillus growth & development, Lactobacillus isolation & purification, Lactobacillus metabolism, Mucins metabolism, Probiotics isolation & purification, Bacteria isolation & purification, Ileum microbiology, Intestinal Mucosa microbiology
- Abstract
In order to perform selective isolation of bacteria tightly bound to the human gut, ileal biopsies of healthy volunteers were treated to wash out the mucus layer and loosely bound bacterial cells. Rod-shaped anaerobic bacteria that had remained attached to the epithelial cells were isolated and identified at the species level. One isolate was identified as belonging to the Bifidobacterium breve species, while all the others were lactobacilli of only two species, Lactobacillus mucosae and Lactobacillus gasseri. Members of these species were found previously in intestinal samples, but their predominance among bacteria strictly associated with the epithelium was not suspected before and suggests that these species may represent a specific subpopulation of tissue-bound bacteria. Physiological analysis indicated that all isolates were able to produce antimicrobials, grow and form biofilm in simulated intestinal fluid after exposure to gastric conditions. Some isolates were able to degrade mucin while none showed cytotoxicity in vitro on HT29 cells. The tight association of the strains isolated with ileal epithelial cells is presumably indicative of a direct interaction with the host cells. For this reason and for the absence of cytotoxicity in vitro, those isolates can be proposed as potential probiotic strains for human use.
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- 2009
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12. Survey of nursing knowledge on bioterrorism.
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De Felice M, Giuliani AR, Alfonsi G, Mosca G, and Fabiani L
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- Adult, Analysis of Variance, Attitude of Health Personnel, Clinical Competence, Curriculum, Education, Nursing, Baccalaureate, Education, Nursing, Continuing, Education, Nursing, Diploma Programs, Education, Nursing, Graduate, Educational Measurement, Health Knowledge, Attitudes, Practice, Health Services Needs and Demand, Humans, Infection Control, Italy, Mass Media, Nursing Education Research, Nursing Staff psychology, Surveys and Questionnaires, Bioterrorism prevention & control, Emergency Nursing education, Emergency Nursing methods, Nursing Staff education, Students, Nursing psychology
- Abstract
With the aim of identifying intervention programmes within the framework of basic and permanent nursing training, we evaluated the knowledge of 187 nurses and nursing students concerning biological emergencies. A questionnaire was used to identify their knowledge of the pathogens that may be used in a terrorist attack and measures for containing them, and their perception of the danger to public health. Analysis of the responses showed that the undergraduates studying for the Triennial Degree were the best informed, and those studying for the specialist degree were the least informed. The question relating to the precautions adopted against transmission of Bacillus anthracis was the most predictive of the total score because it was the only one with a substantial number of correct answers (79/187). It seems that the information derived more from the generalist media than from academic training. We conclude that there is a need to train qualified personnel capable of responding to a possible emergency in terms of both preventive and interventional medicine.
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- 2008
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13. Improvement in the quality of the catering service of a rehabilitation hospital.
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Donini LM, Castellaneta E, De Guglielmi S, De Felice MR, Savina C, Coletti C, Paolini M, and Cannella C
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- Aged, Female, Humans, Length of Stay, Male, Menu Planning, Middle Aged, Taste, Temperature, Food standards, Food Handling methods, Food Handling standards, Food Service, Hospital standards, Patient Satisfaction, Quality Control
- Abstract
Background: Malnutrition due to undernutrition or overnutrition is highly prevalent in hospital in-patients and it decisively conditions patients clinical outcome. One of the most influencing factors of malnutrition in hospitalized patients is--at least in part--the Catering Service Quality., Aim: Is to verify, over a 5 year period, the course of the quality of the institutional Catering Service, verifying the effectiveness of the quality improvement process used., Methods: Quality control was performed by objective (meal order accuracy, proper distribution of food in trolleys, route time from the kitchen to the ward and time of food distribution, food weight and temperature, waste assessment) and subjective assessment (quality was measured by giving the patients a questionnaire after meals)., Results: The survey included: 572 meals and 591 interviews. A significant amount of "qualitative" errors (lack of respect for patient preferences or at the moment of supplying the food trolley) have been found. Over the time and the amount of patients that wasted a considerable amount of the portion served was considerably reduced food temperature have been improved. Also patient satisfaction with menu variability, portion size, temperature and cooking quality improved over time. The overall ratings of meals under observation improved too in fact, positive opinions ranged from 18% in 2002 to 48.3% in 2006., Conclusion: Ongoing research and quality verification, which include all catering service workers, yields a constant improvement in quality. Patients in healthcare settings should receive a service they appreciates, but it should be--at the same time--correct from a nutritional point of view. For this reason, it is necessary a continuous mediation between customers satisfaction and nutritionists work, dieticians and nursing staff. From this point of view the educational approach becomes essential to feed patient compliance to dietetic treatment that will continue after discharge.
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- 2008
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14. Small surface-associated factors mediate adhesion of a food-isolated strain of Lactobacillus fermentum to Caco-2 cells.
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Baccigalupi L, Di Donato A, Parlato M, Luongo D, Carbone V, Rossi M, Ricca E, and De Felice M
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- Anti-Bacterial Agents biosynthesis, Anti-Bacterial Agents pharmacology, Bile Acids and Salts pharmacology, Caco-2 Cells, Food Microbiology, Humans, Hydrogen-Ion Concentration, Lactobacillus classification, Lactobacillus drug effects, Lactobacillus isolation & purification, Lactobacillus metabolism, Adhesins, Bacterial isolation & purification, Adhesins, Bacterial physiology, Bacterial Adhesion physiology, Lactobacillus chemistry, Lactobacillus physiology
- Abstract
In a search for bacteria having putative probiotic activity, we screened a collection of food-isolated microorganisms for the ability to survive at low pH in the presence of bile salts and for the production of antimicrobial compounds active against a number of animal pathogens. Among these, we found a strain that we classified as a member of Lactobacillus fermentum sp., and we further investigated its features. This organism was able to adhere to human enterocyte-like (Caco-2) cells with high efficiency as compared to that of a well known indicator strain. Chromatographic analysis indicated that at least two small (less than 3 kDa) factors were involved in mediating the in vitro interaction of L. fermentum with Caco-2 cells. Adhesion activity could be abolished by mild treatment of the bacterial cells in buffer and rescued by incubating them with either the same buffer after its use in the treatment or with chromatographic fractions containing each of the two factors, which indicated that these factors were loosely associated with the cell wall and that each of them was sufficient to warrant the adhesiveness of L. fermentum to Caco-2 cells. These data are suggestive of a novel mechanism of bacterial adhesion to epithelial cells.
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- 2005
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15. An integrated regulatory network controlling survival and migration in thyroid organogenesis.
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Parlato R, Rosica A, Rodriguez-Mallon A, Affuso A, Postiglione MP, Arra C, Mansouri A, Kimura S, Di Lauro R, and De Felice M
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- Animals, DNA-Binding Proteins genetics, Endoderm cytology, Endoderm metabolism, Forkhead Transcription Factors, Hedgehog Proteins, In Situ Hybridization, Mice, Mice, Inbred Strains, Mice, Knockout, Nuclear Proteins genetics, PAX8 Transcription Factor, Paired Box Transcription Factors, Signal Transduction physiology, Stem Cells cytology, Stem Cells metabolism, Trans-Activators genetics, Transcription Factors genetics, Cell Movement, Cell Survival, DNA-Binding Proteins metabolism, Gene Expression Regulation, Developmental, Morphogenesis, Nuclear Proteins metabolism, Thyroid Gland cytology, Thyroid Gland embryology, Thyroid Gland metabolism, Trans-Activators metabolism, Transcription Factors metabolism
- Abstract
The thyroid gland originates from the ventral floor of the foregut as a thickening of the endodermal cell layer. The molecular mechanisms underlying the early steps of thyroid morphogenesis are not known. Gene targeting experiments have contributed to the identification of several transcription factors, in general playing a role in the proliferation, survival, and migration of the thyroid cell precursors. The experiments reported here analyze the expression of the transcription factors Titf1, Hhex, Pax8, and Foxe1 in the thyroid primordium of null mutants of each of them. We found that most of these transcription factors are linked in an integrated regulatory network, each of them controlling the presence of other members of the network. The expression of Foxe1 is regulated in an intriguing fashion as it is strongly dependent on the presence of Pax8 in thyroid precursor cells, while the expression of the same gene in the pharyngeal endoderm surrounding the primordium is dependent on Sonic hedgehog (Shh)-derived signaling. Moreover, by the generation of mouse mutants expressing Foxe1 exclusively in the thyroid primordium, we provide a better understanding of the role of Foxe1 in these cells in order to acquire the competence to migrate into the underlying mesenchyme. In conclusion, we provide the first evidence of gene expression programs, controlled by a hierarchy of transcription factors expressed in the thyroid presumptive gut domain and directing the progression of thyroid morphogenesis.
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- 2004
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16. A preservation method that allows recovery of intact RNA from tissues dissected by laser capture microdissection.
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Parlato R, Rosica A, Cuccurullo V, Mansi L, Macchia P, Owens JD, Mushinski JF, De Felice M, Bonner RF, and Di Lauro R
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- Animals, Blotting, Northern, Cryopreservation methods, Embryo, Mammalian cytology, Embryo, Mammalian metabolism, Humans, Mice, Polymerase Chain Reaction, RNA genetics, RNA, Messenger genetics, RNA, Messenger isolation & purification, RNA, Messenger metabolism, Sucrose, Thyroid Neoplasms genetics, Thyroid Neoplasms pathology, Dissection methods, Lasers, RNA isolation & purification, RNA metabolism, Tissue Preservation methods
- Abstract
We report a novel method for preparing samples for laser capture microdissection. The procedure described here permits extraction of intact RNA while preserving morphology, thus being suitable both for identification of specific cells and for analysis of their gene expression. The method is applicable to both mouse embryos and human tumors and may improve the preparation of cDNA libraries from specific cell types without interfering with histological diagnosis., ((c)2001 Elsevier Science.)
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- 2002
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17. Biochemical characterization of a clamp-loader complex homologous to eukaryotic replication factor C from the hyperthermophilic archaeon Sulfolobus solfataricus.
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Pisani FM, De Felice M, Carpentieri F, and Rossi M
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- Adenosine Triphosphatases metabolism, Adenosine Triphosphate metabolism, Amino Acid Sequence, Archaeal Proteins genetics, Archaeal Proteins isolation & purification, Base Sequence, Cloning, Molecular, DNA Replication, DNA, Archaeal genetics, DNA, Archaeal metabolism, DNA-Binding Proteins genetics, DNA-Binding Proteins isolation & purification, DNA-Directed DNA Polymerase metabolism, Genes, Archaeal genetics, Holoenzymes chemistry, Holoenzymes genetics, Holoenzymes isolation & purification, Holoenzymes metabolism, Humans, Minor Histocompatibility Antigens, Molecular Sequence Data, Molecular Weight, Multienzyme Complexes chemistry, Multienzyme Complexes genetics, Multienzyme Complexes isolation & purification, Multienzyme Complexes metabolism, Proliferating Cell Nuclear Antigen metabolism, Recombinant Proteins chemistry, Recombinant Proteins genetics, Recombinant Proteins isolation & purification, Recombinant Proteins metabolism, Replication Protein C, Sequence Alignment, Sequence Homology, Archaeal Proteins chemistry, Archaeal Proteins metabolism, DNA-Binding Proteins chemistry, DNA-Binding Proteins metabolism, Homeodomain Proteins, Proto-Oncogene Proteins c-bcl-2, Repressor Proteins, Saccharomyces cerevisiae Proteins, Sulfolobus chemistry, Sulfolobus enzymology, Sulfolobus genetics
- Abstract
Here we report the isolation and characterization of a clamp-loader complex from the thermoacidophilic archaeon Sulfolobus solfataricus (SsoRFC). SsoRFC is a hetero-pentamer composed of polypeptides of 37 kDa (small subunit) and 46 kDa (large subunit), which possess primary structure similarity with human replication factor C p40 and p140 subunits, respectively. The two SsoRFC polypeptides were co-expressed in Escherichia coli and purified as a complex (SsoRFC-complex) that was demonstrated to possess a native M(r) of about 200 kDa and a 4:1 (small to large) subunit stoichiometric ratio. The small subunit was individually expressed in E. coli, purified, and found to form a homo-tetramer (SsoRFC-small; native M(r) 156 kDa), which was also characterized. The SsoRFC-complex, but not SsoRFC-small, highly stimulated the synthetic activity of S. solfataricus B1-type DNA polymerase in reactions containing primed M13mp18 DNA, ATP, and either of the two poliferating cell nuclear antigen-like processivity factors of S. solfataricus (039p and 048p). Both SsoRFC-small and -complex were able to hydrolyze ATP, but only the ATPase activity of the holo-enzymatic assembly was activated by primed DNA templates, such as poly(dA)-oligo(dT). As measured by nitrocellulose filter binding assays, SsoRFC-complex bound poly(dA)-oligo(dT), but not the unprimed homopolymer, whereas SsoRFC-small was devoid of any DNA-binding activity. The peculiar properties of this archaeal clamp-loader complex and their significance for the understanding of the DNA replication process in Archaea are discussed., (Copyright 2000 Academic Press.)
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- 2000
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18. On the fate of ingested Bacillus spores.
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Spinosa MR, Braccini T, Ricca E, De Felice M, Morelli L, Pozzi G, and Oggioni MR
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- Administration, Oral, Animals, Bacillus drug effects, Bacillus subtilis drug effects, Bacillus subtilis physiology, Bile Acids and Salts pharmacology, Deoxycholic Acid pharmacology, Female, Lymph Nodes microbiology, Mice, Mice, Inbred BALB C, Microbial Sensitivity Tests, Spores, Bacterial drug effects, Taurodeoxycholic Acid pharmacology, Bacillus physiology, Intestines microbiology, Probiotics, Spores, Bacterial physiology
- Abstract
Spores of various Bacillus species, including B. subtilis, B. cereus and B. clausii, are used as probiotics, although they are generally absent from the normal microflora of man. We used two nonpathogenic Bacillus species, B. subtilis and B. clausii, to follow the fate of spores inoculated intragastrically in mice. We did not find detectable amounts of vegetative cells in intestinal samples, probably because of high toxicity of the conjugated bile salt taurodeoxycholic acid against Bacillus species. Both spores and cells were detected in the lymph nodes and spleen of one mouse. Our results indicate that Bacillus is present in the intestinal tract solely as spores and that nonpathogenic Bacillus spores may germinate in lymphoid organs, a finding reminiscent of B. anthracis germination in macrophages. These results indicate that any claimed probiotic effect of B. subtilis should be due to spores or, alternatively, to vegetative growth outside the intestine.
- Published
- 2000
- Full Text
- View/download PDF
19. Two DNA polymerase sliding clamps from the thermophilic archaeon Sulfolobus solfataricus.
- Author
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De Felice M, Sensen CW, Charlebois RL, Rossi M, and Pisani FM
- Subjects
- Amino Acid Sequence, Animals, Archaeal Proteins genetics, Archaeal Proteins isolation & purification, Cloning, Molecular, DNA Replication, Escherichia coli, Humans, Molecular Sequence Data, Proliferating Cell Nuclear Antigen chemistry, Rabbits, Sequence Homology, Amino Acid, Sulfolobus genetics, Sulfolobus metabolism, Archaeal Proteins metabolism, DNA-Directed DNA Polymerase metabolism, Sulfolobus enzymology
- Abstract
Herein, we report the identification and characterization of two DNA polymerase processivity factors from the thermoacidophilic archaeon Sulfolobus solfataricus. They, referred to as 039p (244 amino acid residues, 27 kDa) and 048p (249 amino acid residues, 27 kDa), present significant primary structure similarity to eukaryotic proliferating cell nuclear antigen (PCNA). We demonstrate that both 039p and 048p form oligomers in solution and are able to substantially activate the synthetic activity of the single-subunit family B DNA polymerase from S. solfataricus (Sso DNA pol B1) on poly(dA)-oligo(dT) as a primer-template. This stimulatory effect is the result of enhanced DNA polymerase processivity, as indicated by the analysis of the elongation products on polyacrylamide gels. Activation of Sso DNA pol B1 synthetic activity was also observed on linear primed single-stranded M13 mp18 DNA as a template. By immunoblot analysis using specific rabbit antisera, 039p and 048p were both detected in the logarithmic and stationary phases of S. solfataricus growth curve. This is the first report of the identification and biochemical characterization of two distinct DNA polymerase processivity factors from the same organism. The significance of these findings for the understanding of the DNA replication process in Archaea is discussed., (Copyright 1999 Academic Press.)
- Published
- 1999
- Full Text
- View/download PDF
20. Molecular genetics of congenital hypothyroidism.
- Author
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Macchia PE, De Felice M, and Di Lauro R
- Subjects
- Animals, Humans, Mice, Thyroid Gland metabolism, Thyroid Gland pathology, Thyroid Hormones biosynthesis, Congenital Hypothyroidism, Hypothyroidism genetics
- Abstract
Congenital thyroid gland defects - resulting in reduced production of the hormones triiodothyronine (T3) and thyroxine (T4) - can be a consequence of either reduced or absent thyroid tissue (thyroid dysgenesis) or, less frequently, of impairment in the biochemical mechanisms responsible for hormone biosynthesis (thyroid dyshormonogenesis). Recent studies have revealed how mutations in the genes encoding either transcription factors or the thyroid stimulating hormone receptor cause, in humans or in mouse models, thyroid dysgenesis. This demonstrates, for the first time, the heritability of this condition. New genes responsible for thyroid dyshormonogenesis have also been discovered.
- Published
- 1999
- Full Text
- View/download PDF
21. Utilization of cellobiose and other beta-D-glucosides in Agrobacterium tumefaciens.
- Author
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Marasco R, Lago CT, and De Felice M
- Subjects
- Cyclic AMP metabolism, Depression, Chemical, Electrophoresis, Polyacrylamide Gel, Glucose metabolism, In Vitro Techniques, beta-Glucosidase chemistry, Agrobacterium tumefaciens metabolism, Cellobiose metabolism, Glucosides metabolism, beta-Glucosidase metabolism
- Abstract
Agrobacterium tumefaciens strain C58 was able to utilize carbon from cellobiose and some other beta-D-glucosides as efficiently as from glucose. beta-D-glucoside utilization was partially inducible and the induction was subject to catabolite repression by glucose, independently of the presence of cyclic AMP in the medium. It was also independent of Ti plasmid-encoded functions. beta-D-glucosides were hydrolysed by a single, cytoplasmic and constitutively expressed beta-glucosidase, which was active on non-phosphorylated substrates and insensitive to glucose inhibition.
- Published
- 1995
- Full Text
- View/download PDF
22. Acetohydroxy acid synthase and threonine deaminase activities, and the biosynthesis of isoleucine-leucine-valine in Streptococcus bovis.
- Author
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Basso AL, Ricca E, Caruso C, Ferrara L, and De Felice M
- Subjects
- Butyrates metabolism, Streptococcus bovis enzymology, Streptococcus bovis growth & development, Valine biosynthesis, Acetolactate Synthase metabolism, Isoleucine biosynthesis, Leucine biosynthesis, Streptococcus bovis metabolism, Threonine Dehydratase metabolism
- Abstract
Acetohydroxy acid synthase (AHAS) and threonine deaminase (TD) activities were found in Streptococcus bovis and shown to be involved in the biosynthesis of the branched chain amino acids isoleucine, leucine and valine. Apparent lack of repression of AHAS synthesis by the end-products and reduced sensitivity of S. bovis growth to analogues of the branched chain amino acids suggested that secretion of isoleucine, leucine and valine in the growth medium may be a consequence of the regulatory features of AHAS. A glycyl-leucine-resistant mutant with reduced TD activity secreted a reduced amount of isoleucine and an increased amount of valine, which might be a result of the reduced rate of synthesis of the isoleucine precursor alpha-ketobutyrate and of a consequent preferential carbon flow through the valine branch of the pathway.
- Published
- 1993
- Full Text
- View/download PDF
23. Identification and characterization of a T cell growth inhibitory factor produced by K562 erythromyeloid cells.
- Author
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De Felice M, Bond HM, Pizzano R, Turco MC, Valerio G, Lamberti A, Carandente Giarrusso P, and Venuta S
- Subjects
- Humans, Isoelectric Point, Lymphocyte Activation drug effects, Molecular Weight, Suppressor Factors, Immunologic pharmacology, T-Lymphocytes drug effects, Tumor Cells, Cultured, Leukemia, Erythroblastic, Acute metabolism, Suppressor Factors, Immunologic isolation & purification
- Abstract
Cells of the human erythroleukemia cell line K562 constitutively secrete a factor that inhibits human T lymphocyte proliferation induced via CD3/Ti. The factor, termed K-TIF (K562-derived T cell inhibitory factor) is produced in either the presence or absence of fetal calf serum in cultures of K562 cells and can be precipitated by 70% NH4SO4. Gel filtration chromatography on Superose 12 resin by FPLC showed that the inhibitory factor has a molecular weight of approximately 30-35 kDa. A protein of this size, metabolically labeled with [35S]methionine, specifically bound human peripheral blood mononuclear cells. Chromatofocusing with Mono P by FPLC (pH gradient 7.2-5) indicates that the inhibitory factor has an isoelectric point of 6.0-6.4.
- Published
- 1991
- Full Text
- View/download PDF
24. T-cell malignancies with mature phenotypes: altered cell cycle regulation by HLA class I molecules.
- Author
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Turco MC, Alfinito F, De Felice M, Lamberti A, Ferrone S, and Venuta S
- Subjects
- Antibodies, Monoclonal immunology, Antigens, Differentiation, T-Lymphocyte immunology, CD2 Antigens, CD3 Complex, Cell Division, Gene Expression, Histocompatibility Antigens Class I immunology, Histocompatibility Antigens Class I physiology, Humans, Leukemia, Prolymphocytic pathology, Leukemia, T-Cell immunology, Leukemia, T-Cell pathology, Mitogens immunology, Phenotype, Protein Kinase C antagonists & inhibitors, Receptors, Antigen, T-Cell immunology, Receptors, Immunologic immunology, Tumor Cells, Cultured, Leukemia, T-Cell genetics
- Abstract
Soluble anti-HLA class I monoclonal antibodies (MoAbs) modulate normal T-lymphocyte proliferation induced via the CD3/Ti and the CD2 pathway, but do not induce proliferation of normal T lymphocytes in the absence of additional mitogenic stimuli. In this report, we show that anti-HLA class I MoAbs induce DNA synthesis in peripheral blood mononuclear cells from a patient with a CD4+CD8+T-prolymphocytic leukemia (T-PLL) and from a patient with a CD4-CD8+ T-chronic lymphocytic leukemia (T-CLL), in the absence of detectable additional mitogenic stimuli. Proliferation of leukemic T cells is induced by both whole Igs and Fab' fragments of anti-HLA class I MoAbs, arguing in favor of their direct interactions with the proliferating cells as the mechanism underlying the mitogenic effect. This interpretation is also supported by the ability of anti-HLA class I MoAbs to induce proliferation of leukemic T-cell preparations, depleted of accessory cells. DNA synthesis in T-CLL and T-PLL cells is preceded by expression of G1-specific messenger RNAs, ie. c-myc, 2F1, Tac, and interferon-gamma, in activated cells. Cell proliferation is inhibited by the protein kinase C inhibitor H7, indicating that activation of this enzyme is required for the mitogenic effect of anti-HLA class I MoAbs. The latter inhibit the proliferation of T-CLL cells as well as that of normal T cells stimulated with anti-CD3 MoAbs and enhance that of both types of cells stimulated with anti-CD2 MoAbs. In addition, anti-HLA class I MoAb Q6/64 in combination with anti-CD2 MoAb 9.6 or MoAb 9-1 induces proliferation of leukemic T cells to a greater extent than the individual MoAbs, but is not mitogenic for normal T cells. Anti-HLA class I MoAbs restore the cytolytic activity of T-CLL cells that is lost after 5 days of incubation of control medium, suggesting that HLA class I antigens may mediate a signal contributing to the activation state. The present results indicate that leukemic T-cell proliferation can be triggered via HLA class I molecules and suggest a potential role for these antigens in the in vivo growth of malignant clones.
- Published
- 1991
25. Inhibition by anti-HLA class I mAb of IL-2 and IL-2 receptor synthesis in lymphocytes stimulated with PHA-P.
- Author
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De Felice M, Turco MC, Costanzo F, Corbo L, Ferrone S, and Venuta S
- Subjects
- Humans, Interleukin-2 genetics, Lymphocyte Activation, RNA, Messenger analysis, Receptors, Interleukin-2 genetics, Antibodies, Monoclonal immunology, Histocompatibility Antigens Class I immunology, Interleukin-2 biosynthesis, Lymphocytes immunology, Phytohemagglutinins pharmacology, Receptors, Interleukin-2 biosynthesis
- Abstract
Inhibition by anti-HLA Class I monoclonal antibody (mAb) Q6/64 of phytohemagglutinin (PHA)-P-induced peripheral blood mononuclear cell (PMBC) proliferation is associated with a reduction of Tac expression and interleukin 2 (IL-2) secretion. To analyze the mechanism(s) underlying the latter phenomena, the Tac gene and IL-2 gene transcription was analyzed by a nuclear transcription assay. No synthesis of Tac and IL-2 mRNA was detected in PBMC stimulated with PHA-P in the presence of mAb Q6/64. In conjunction with our recently published data, these results indicate that the blocking by anti-HLA Class I mAb of PHA-P-induced PBMC proliferation reflects an inhibitory effect within the signal transduction pathway leading to transcriptional activation of IL-2 and IL-2 receptor genes.
- Published
- 1990
- Full Text
- View/download PDF
26. The effect of TRH and a related tripeptide, L-N-(2-oxopiperidin-6-yl-carbonyl)-L-histidyl-L-thiazolidine-4-carboxamide (MK-771, OHT), on the depressant action of barbiturates and alcohol in mice and rats.
- Author
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Porter CC, Lotti VJ, and de Felice MJ
- Subjects
- Animals, Avoidance Learning drug effects, Behavior, Animal drug effects, Body Temperature drug effects, Dose-Response Relationship, Drug, Female, Methohexital pharmacology, Mice, Pentobarbital pharmacology, Rats, Barbiturates pharmacology, Ethanol pharmacology, Learning drug effects, Sleep drug effects, Thyrotropin-Releasing Hormone analogs & derivatives, Thyrotropin-Releasing Hormone pharmacology
- Published
- 1977
- Full Text
- View/download PDF
27. Lack of a role of monocytes in the inhibition by monoclonal antibodies to monomorphic and polymorphic determinants of HLA class I antigens of PHA-P-induced peripheral blood mononuclear cell proliferation.
- Author
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De Felice M, Turco MC, Corbo L, Carandente Giarrusso P, Lamberti A, Valerio G, Temponi M, Costanzo F, Ferrone S, and Venuta S
- Subjects
- Antigens, Differentiation, T-Lymphocyte immunology, CD2 Antigens, CD3 Complex, Humans, Interleukin-2 metabolism, Phytohemagglutinins pharmacology, Receptors, Antigen, T-Cell immunology, Receptors, Immunologic immunology, Receptors, Interleukin-2 analysis, T-Lymphocytes, Regulatory physiology, Antibodies, Monoclonal immunology, Epitopes immunology, Histocompatibility Antigens Class I immunology, Lymphocyte Activation, Monocytes physiology
- Abstract
This study aimed at characterizing the mechanism(s) underlying the regulatory role of distinct determinants of HLA Class I antigens in PHA-P-induced T cell proliferation and the involvement of monocytes in this phenomenon. The anti-HLA-A2,A28 monoclonal antibodies (MoAb) CR11-351, the MoAb Q6/64 to a determinant restricted to the gene products of the I antigens HLA-B locus, and the MoAb CR10-215 and W6/32 to distinct monomorphic determinants of HLA Class I antigens were found to inhibit PHA-P-induced peripheral blood mononuclear cell (PBMC) proliferation in a dose-dependent fashion. The inhibition is specific and reflects neither inhibition of PHA-P binding to cells nor a toxic effect of the anti-HLA Class I MoAb. The latter differed in the concentration required to induce inhibition, in the influence of the concentration of PHA-P used as mitogen, in the differential effect on the donors used as a source of PBMC, and/or in the requirement of the Fc portion to induce inhibition. At variance with the information in the literature, the inhibitory effect of anti-HLA Class I MoAb on PHA-P-induced PBMC proliferation neither reflected their interaction with accessory cells nor was mediated by suppressor factors released by monocytes stimulated with PHA-P in the presence of anti-HLA Class I MoAb. Therefore, the regulatory role of HLA Class I antigens in T cell proliferation is not likely to be mediated by monocytes and/or factors released from them, but may reflect an involvement of these molecules in T cell activation pathways.
- Published
- 1989
- Full Text
- View/download PDF
28. Proliferative pathways in CD1- CD3+ CD4+ CD8+ T-prolymphocytic leukemic cells: analysis with monoclonal antibodies and cytokines.
- Author
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Turco MC, De Felice M, Alfinito F, Lamberti A, Costanzo F, Giordano M, Martinelli V, Rotoli B, Ferrone S, and Venuta S
- Subjects
- Cell Differentiation, Cells, Cultured, Humans, Kinetics, Microscopy, Electron, Recombinant Proteins pharmacology, Reference Values, T-Lymphocytes drug effects, T-Lymphocytes ultrastructure, Tetradecanoylphorbol Acetate pharmacology, Antibodies, Monoclonal, Antigens, CD analysis, Interleukin-1 pharmacology, Interleukin-2 pharmacology, Interleukin-6 pharmacology, Leukemia, Prolymphocytic immunology, Lymphocyte Activation drug effects, T-Lymphocytes immunology
- Abstract
The antigenic profile and the proliferative pathways in leukemic cells from the patient TRT with T-prolymphocytic leukemia (T-PLL) were analyzed using monoclonal antibodies (MoAbs) and cytokines. T-PLL cells expressed the phenotype CD1- CD3+ CD4+ CD8+. Incubation with the differentiating agent phorbol-12-myristate-13-acetate markedly increased the percentage of cells with the CD4- CD8+ phenotype, suggesting that leukemic cells were already committed towards a differentiated element with the CD4- CD8+ phenotype. T-PLL cells were induced to proliferate by anti-CD2 MoAb 9-1 + 9.6 and by anti-CD3 MoAb OKT3. The two pathways exhibited normal functional interactions and were susceptible to modulation by anti-HLA class I MoAbs. These results indicate that regulation of cell proliferation was preserved to a significant extent in the T-PLL cells analyzed. At variance with normal resting T cells that require previous activation to proliferate when incubated with interleukin-1 (IL-1) or interleukin-2 (IL-2), T-PLL cells proliferated vigorously when incubated with either interleukin. Furthermore, T-PLL cells proliferated when incubated with immune interferon (IFN-gamma). The latter finding parallels the enhancement by IFN-gamma of the proliferative response of lectin-activated murine T lymphocytes. These results suggest that T-PLL cells, which express a high constitutive level of c-myc mRNA, may be in an activated state. The antigenic phenotype and the characteristics of the proliferative pathways of T-PLL cells from the patient TRT are compatible with the possibility that they may be derived from an intermediate thymocyte.
- Published
- 1989
29. Chromatographic detection of the acetohydroxy acid synthase isoenzymes of Escherichia coli K-12.
- Author
-
La Cara F and De Felice M
- Subjects
- Chromatography, DEAE-Cellulose, Genotype, Species Specificity, Acetolactate Synthase isolation & purification, Escherichia coli enzymology, Isoenzymes isolation & purification, Oxo-Acid-Lyases isolation & purification
- Published
- 1979
- Full Text
- View/download PDF
30. The acetohydroxy acid synthase III isoenzyme of Escherichia coli K-12: regulation of synthesis by leucine.
- Author
-
De Felice M and Levinthal M
- Subjects
- Cell Division, Enzyme Induction drug effects, Escherichia coli drug effects, Genotype, Kinetics, Species Specificity, Threonine Dehydratase metabolism, Acetolactate Synthase biosynthesis, Escherichia coli enzymology, Leucine pharmacology, Oxo-Acid-Lyases biosynthesis
- Published
- 1977
- Full Text
- View/download PDF
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