233 results on '"Ding, K."'
Search Results
2. Two-dimensional simulation of two-sided laser shock peening on thin sections
- Author
-
Ding, K., primary and Ye, L., additional
- Published
- 2006
- Full Text
- View/download PDF
3. Simulation methodology
- Author
-
Ding, K., primary and Ye, L., additional
- Published
- 2006
- Full Text
- View/download PDF
4. Simulation of laser shock peening on a curved surface
- Author
-
Ding, K., primary and Ye, L., additional
- Published
- 2006
- Full Text
- View/download PDF
5. Physical and mechanical mechanisms of laser shock peening
- Author
-
Ding, K., primary and Ye, L., additional
- Published
- 2006
- Full Text
- View/download PDF
6. General introduction
- Author
-
Ding, K., primary and Ye, L., additional
- Published
- 2006
- Full Text
- View/download PDF
7. A first step toward functionally graded plasticity in porous materials
- Author
-
Ding, K., primary and Weng, G.J., additional
- Published
- 1998
- Full Text
- View/download PDF
8. The role of ITO resistivity on current spreading and leakage in InGaN/GaN light emitting diodes
- Author
-
V. Avrutin, M. Elçi, Atilla Aydinli, Kai Ding, Hadis Morkoç, N. Sheremet, Ümit Özgür, Ismail Altuntas, M. Genç, V. N. Sheremet, Uludağ Üniversitesi/Mühendislik Fakültesi/Elektrik ve Elektronik Mühendisliği Bölümü., Aydınlı, Atilla, ABI-7535-2020, [Sheremet, V. -- Elci, M. -- Aydinli, A.] Bilkent Univ, Dept Phys, Adv Res Labs, TR-06800 Ankara, Turkey -- [Genc, M.] Optoelectron R&D Ctr, Ermaksan, TR-16065 Bursa, Turkey -- [Sheremet, N.] Natl Acad Sci Ukraine, Inst Phys, UA-03680 Kiev, Ukraine -- [Aydinli, A.] Uludag Univ, Dept Elect & Elect Engn, TR-16059 Bursa, Turkey -- [Altuntas, I.] Cumhuriyet Univ, Dept Nanotechnol Engn, TR-58140 Sivas, Turkey -- [Ding, K. -- Avrutin, V. -- Ozgur, U. -- Morkoc, H.] Virginia Commonwealth Univ, Sch Engn, Dept Elect & Comp Engn, Richmond, VA 23284 USA, Sheremet, Volodymyr -- 0000-0003-0840-5312, Sheremet, Nina -- 0000-0001-6955-1095, Genc, Muhammet -- 0000-0003-1887-3582, and Ding, Kai -- 0000-0003-4791-4742
- Subjects
Materials science ,Passivation ,Contacts ,02 engineering and technology ,01 natural sciences ,law.invention ,Finite conductivity ,Electrical resistivity and conductivity ,law ,0103 physical sciences ,General Materials Science ,Physics, condensed matter ,Indium Tin Oxide ,InGaN/GaN multiple quantum well ,Ingan/gan lightemitting diodes (LEDs) ,Electrical and Electronic Engineering ,Current spreading ,Semiconductor quantum wells ,Leakage (electronics) ,010302 applied physics ,Contact pad ,Indium alloys ,business.industry ,Physics ,LED ,Tin oxides ,Indium tin oxide ,Gallium alloys ,Semiconducting indium compounds ,Current transfer ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Semiconductor junctions ,Light emitting diodes ,InGaN/GaN ,Flip Chip ,Extraction Efficiency ,Light Emitting Diodes ,Semiconductors ,Electrode ,Inter-digitated electrodes ,Optoelectronics ,Transparent conductive electrodes ,Light output power ,0210 nano-technology ,business ,Current density ,Efficiency droop ,Light-emitting diode - Abstract
WOS: 000415768800128, The effect of a transparent ITO current spreading layer on electrical and light output properties of blue InGaN/GaN light emitting diodes (LEDs) is discussed. When finite conductivity of ITO is taken into account, unlike in previous models, the topology of LED die and contacts are shown to significantly affect current spreading and light output characteristics in top emitting devices. We propose an approach for calculating the current transfer length describing current spreading. We show that an inter-digitated electrode configuration with distance between the contact pad and the edge of p-n junction equal to transfer length in the current spreading ITO layer allows one to increase the optical area of LED chip, as compared to the physical area of the die, light output power, and therefore, the LED efficiency for a given current density. A detailed study of unpassivated LEDs also shows that current transfer lengths longer than the distance between the contact pad and the edge of p-n junction leads to increasing surface leakage that can only be remedied with proper passivation. (C) 2017 Elsevier Ltd. All rights reserved., TUBITAK [113G042]; Scientific and Technological Research Council of Turkey (TUBITAK), This work has been performed with the support of TUBITAK (Grant no: 113G042). Ismail Altuntas, acknowledges the Ph.D. Grant support from the Scientific and Technological Research Council of Turkey (TUBITAK).
- Published
- 2017
9. Dual amplification dynamic DNA network system for CRISPR/Cas12a based p53 gene detection.
- Author
-
Wang T, Ding K, Wang X, Wang Z, Liu G, Zang Y, Lin S, Zhou H, and Wang Q
- Subjects
- Humans, Nucleic Acid Amplification Techniques, Biosensing Techniques methods, DNA chemistry, DNA genetics, Limit of Detection, Genes, p53, Nucleic Acid Hybridization, Tumor Suppressor Protein p53 genetics, CRISPR-Cas Systems genetics
- Abstract
Background: It is estimated that over 50 % of human cancers are caused by mutations in the p53 gene. Early sensitive and accurate detection of the p53 gene is important for diagnosis of cancers in the early stage. However, conventional detection techniques often suffer from strict reaction conditions, or unsatisfied sensitivity, so we need to develop a new strategy for accurate detection of p53 gene with smart designability, multiple signal amplification in mild reaction conditions., Results: In this study, CRISPR/Cas system is exploited in entropy-driven catalysis (EDC) and hybridization chain reaction (CHA) dual signal amplification sensing strategies. The products of both reactions can efficiently and separately activate CRISPR/Cas12a which greatly amplifies the fluorescent signal. The method has good linearity in p53 detection with the concentration ranged from 0.1 fM to 0.5 pM with ultra-low detection limit of 0.096 fM. It also showed good performance in serum, offering potentials for early disease detection., Significance: The designed dual amplification dynamic DNA network system exhibits an ultra-sensitive fluorescence biosensing for p53 gene identification. The method is simple to operate and requires only one buffer for the experiment, and meanwhile shows smart designability which could be used for a wide range of markers. Thus, we believe the present work will provide a potential tool for the construction and development of sensitive fluorescent biosensors for diseases., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
10. Toxicokinetics of microplastics in Macrobrachium nipponense and their impact on the bioavailability of loaded pollutants.
- Author
-
Zhang L, Lu G, Ling X, Yan Z, Liu J, and Ding K
- Abstract
Microplastics (MPs) have unique toxicokinetic (TK) processes that differ from those of soluble pollutants. This study investigated the ingestion, migration, accumulation, and clearance of environmental aging MPs in the Japanese swamp shrimp (Macrobrachium nipponense). The concentrations of plastic additives and personal care products adsorbed onto MPs in natural river water were determined, and TK models for MPs and MPs-loaded pollutants were developed. Results showed that the formation of surface biofilms and alterations in the distribution of MPs in waters caused by environmental aging affect MPs bioavailability, which is mainly related to the feeding habits of shrimp. The decrease in MPs particle size caused by biological digestion and the increase in the number of oxygen-containing functional groups caused by environmental aging affect the TK process of MPs. The TK model of MPs-loaded pollutants revealed the cleaning effect of shrimp on pollutants adsorbed onto MPs during swallowing and spitting MPs. This cleaning effect significantly increases the bioavailability of MPs-associated pollutants in aquatic environments. This study provides a new perspective for understanding the interactions between environmental MPs and their associated pollutants in aquatic ecosystems., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
11. Adsorption and desorption of parachlormetaxylenol by aged microplastics and molecular mechanism.
- Author
-
Zhang J, Lu G, Wang M, Zhang P, and Ding K
- Abstract
The addition of active ingredients such as antibacterial agent and non-active ingredients such as plastic microspheres (MPs) in personal care products (PCPs) are the common pollutants in the aquatic environment, and their coexistence poses potential threat to the aquatic ecosystem. As a substitute for the traditional antibacterial ingredients triclosan and triclocarban, the usage of parachlormetaxylenol (PCMX) is on the rise and is widely used in PCPs. In this study, the adsorption and desorption behaviors of PCMX were investigated with two typical MPs, polyvinyl chloride (PVC) and polyethylene (PE), and the effects of different aging modes and molecular mechanisms were explored through batch experiments and density functional theory calculation. Both laboratory aging and field aging resulted in surface wrinkles of MPs, along with an increased proportion of oxygen-containing functional groups (CO, -OH). At the same aging time, the degree of laboratory aging was stronger than that of field aging, and the aging degree of PVC was greater that of PE. The aging process enhanced the adsorption capacity of MPs for PCMX. The equilibrium adsorption capacity of PVC increased from 3.713 mg/g (virgin) to 3.823 mg/g (field aging) and 3.969 mg/g (laboratory aging), while that of PE increased from 3.509 mg/g to 3.879 mg/g and 4.109 mg/g, respectively. Meanwhile, aging also resulted in an increase in the desorption capacity of PCMX from PVC and PE. Oxygen-containing functional groups in aged MPs could serve as adsorption sites for PCMX and improved the electrostatic adsorption capacity. Oxygen-containing groups generated on the surface of aged MPs formed hydrogen bonding with the phenolic hydroxyl groups of PCMX, which became the main driving force for adsorption. Our results reveal the potential impact and mechanism of aging on the adsorption of PCMX by MPs, which provides new insights for the interaction mechanism between environmental MPs and associated contaminants., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
12. Cucurbitacin I exerts its anticancer effects by inducing cell cycle arrest via the KAT2a-ube2C/E2F1 pathway and inhibiting HepG2-induced macrophage M2 polarization.
- Author
-
Gong X, Liu Y, Liang K, Chen Z, Ding K, Qiu L, Wei J, and Du H
- Abstract
Hepatocellular carcinoma (HCC) is one of the most common malignancies globally, particularly prevalent in China, where it accounts for nearly half of the world's new cases and deaths each year, but has limited therapeutic options. This study systematically investigated the impact of cucurbitacin I on HCC cell lines including SK-Hep-1, Huh-7, and HepG2. The results revealed that cucurbitacin I not only inhibited cell proliferation, cell migration and colony formation, but also induced apoptosis in HCC cells. The apoptotic induction was accompanied by a decrease in the expression of the anti-apoptotic factor B-cell lymphoma 2 (Bcl2), and an elevation in the expression levels of pro-apoptotic factors, including tumor protein p53 (P53), bcl2 associated X-apoptosis regulator (Bax), and caspase3 (Cas3). Additionally, cucurbitacin I caused cell cycle arrest by modulating the lysine acetyltransferase 2A (KAT2A)-E2F transcription factor 1 (E2F1)/Ubiquitin-conjugating enzyme E2 C (UBE2C) signaling axis. In terms of regulation on tumor microenvironment, cucurbitacin I was demonstrated the ability to inhibit HCC cell-induced M2 polarization of macrophages. This comprehensive study unveils the multifaceted anti-cancer mechanisms of cucurbitacin I, providing robust support for its potential application in the treatment of HCC, offering new avenues for the future development of HCC treatment strategies., Competing Interests: Declaration of competing interest All authors disclosed no relevant relationships., (Copyright © 2024. Published by Elsevier Inc.)
- Published
- 2024
- Full Text
- View/download PDF
13. The demethylase ALKBH5 mediates ZKSCAN3 expression through the m 6 A modification to activate VEGFA transcription and thus participates in MNNG-induced gastric cancer progression.
- Author
-
Wang Q, Huang Y, Jiang M, Tang Y, Wang Q, Bai L, Yu C, Yang X, Ding K, Wang W, Bai J, and Chen Y
- Subjects
- Humans, Animals, Cell Line, Tumor, Mice, Nude, Male, Gene Expression Regulation, Neoplastic drug effects, Carcinogens toxicity, Mice, Inbred BALB C, Mice, Stomach Neoplasms pathology, Stomach Neoplasms metabolism, Stomach Neoplasms genetics, Stomach Neoplasms chemically induced, AlkB Homolog 5, RNA Demethylase metabolism, AlkB Homolog 5, RNA Demethylase genetics, Vascular Endothelial Growth Factor A metabolism, Vascular Endothelial Growth Factor A genetics, Adenosine analogs & derivatives, Adenosine metabolism, Methylnitronitrosoguanidine toxicity, Cell Movement drug effects, Disease Progression
- Abstract
N-Nitroso compounds (NOCs) are recognized as important factors that promote gastric cancer development, but the specific effects and potential mechanisms by which NOC exposure promotes gastric cancer are still poorly understood. In this study, we explored the effects and potential molecular mechanisms of NOCs on the promotion of gastric cancer using methylnitronitrosoguanidine (MNNG), a classical direct carcinogen of NOC. The results of in vivo and in vitro experiments showed that chronic and low-concentration MNNG exposure significantly promoted the malignant progression of tumors, including cell migration, cell invasion, vasculogenic mimicry (VM) formation, cell spheroid formation, stem cell-like marker expression, and gastric cancer growth and metastasis. Mechanistically, we revealed that demethylase ALKBH5 regulated the level of the N6‑methyladenosine (m6A) modification in the 3'UTR and CDS region of the ZKSCAN3 mRNA to promote ZKSCAN3 expression, mediated the binding of ZKSCAN3 to the VEGFA promoter region to regulate VEGFA transcription, and participated in MNNG-induced gastric cancer cell migration, invasion, VM formation, cell spheroid formation, stem cell-like marker expression and ultimately gastric cancer progression. In addition, our study revealed that ALKBH5-ZKSCAN3-VEGFA signaling was significantly activated during MNNG-induced gastric carcinogenesis, and further studies in gastric cancer patients showed that ALKBH5, ZKSCAN3, and VEGFA expression were upregulated in cancers compared with paired gastric mucosal tissues, that ALKBH5, ZKSCAN3, and VEGFA could serve as important biomarkers for determining patient prognosis, and that the molecular combination showed greater prognostic value. These findings provide a theoretical basis for developing gastric cancer interventions for NOCs and for determining gastric cancer progression., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
14. Application of phosphogypsum and phosphate-solubilizing fungi to Pb remediation: From simulation to in vivo incubation.
- Author
-
Meng L, Ding K, Qiu Y, Chen Y, Huo H, Yu D, Tian D, and Li Z
- Subjects
- Environmental Restoration and Remediation methods, Lead metabolism, Phosphorus metabolism, Calcium Sulfate, Aspergillus niger metabolism, Biodegradation, Environmental, Phosphates metabolism, Soil Pollutants metabolism
- Abstract
Phosphogypsum (PG) is the produced solid waste during phosphorus (P) extraction from phosphate rocks. PG is featured by its abundant PO
4 3- and SO4 2- . This study investigated the utilization of PG as a material for lead (Pb) remediation, with the assistance of functional fungus. Aspergillus niger (A. niger) is a typical phosphate-solubilizing fungi (PSF), which has high ability to secret organic acids. Oxalic acid is its major secreted organic acid, which is often applied to enhance the P release from phosphate minerals. In this study, synthetic oxalic acid increased the immobilization rate of Pb2+ . Moreover, this study elucidated that oversupply of PG promoted the formation of anglesite (K2+ . Simultaneously, A. niger did not induce evidently elevation water-soluble fluorine (F) as PG contained abundant Ca2+ , relatively unstable), whereas the formation of lead oxalate (Ksp = 1.6 × 10-8 , relatively unstable), whereas the formation of lead oxalate (Ksp = 4.8 × 10-10 , relatively stable) was reduced. This study hence shed a bright light on the sustainable utilization of PG for fungus-assisted remediation of heavy metals., Competing Interests: Declaration of competing interest The authors have no conflict of interest., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
15. Design, synthesis and evaluation of thieno[3,2-d]pyrimidine derivatives as novel potent CDK7 inhibitors.
- Author
-
Zhang H, Lin G, Jia S, Wu J, Zhang Y, Tao Y, Huang W, Song M, Ding K, Ma D, and Fan M
- Subjects
- Humans, Structure-Activity Relationship, Molecular Structure, Animals, Cell Proliferation drug effects, Dose-Response Relationship, Drug, Drug Screening Assays, Antitumor, Cell Line, Tumor, Rats, Pyrimidines chemistry, Pyrimidines chemical synthesis, Pyrimidines pharmacology, Pyrimidines pharmacokinetics, Cyclin-Dependent Kinases antagonists & inhibitors, Cyclin-Dependent Kinases metabolism, Protein Kinase Inhibitors chemical synthesis, Protein Kinase Inhibitors pharmacology, Protein Kinase Inhibitors chemistry, Protein Kinase Inhibitors pharmacokinetics, Drug Design, Antineoplastic Agents pharmacology, Antineoplastic Agents chemical synthesis, Antineoplastic Agents chemistry, Cyclin-Dependent Kinase-Activating Kinase
- Abstract
The targeting of cyclin-dependent kinase 7 (CDK7) has become a highly desirable therapeutic approach in the field of oncology due to its dual role in regulating essential biological processes, encompassing cell cycle progression and transcriptional control. We have previously identified a highly selective thieno[3,2-d]pyrimidine-based CDK7 inhibitor with demonstrated efficacy and safety in animal model. In this study, we sought to optimize the thieno[3,2-d]pyrimidine core to discover a novel series of CDK7 inhibitors with improved potency and pharmacokinetic (PK) properties. Through extensive structure-activity relationship (SAR) studies, compound 20 has emerged as the lead candidate due to its potent inhibitory activity against CDK7 and remarkable efficacy on MDA-MB-453 cells, a representative triple negative breast cancer (TNBC) cell line. Furthermore, 20 has demonstrated favorable oral bioavailability and exhibited highly desirable pharmacokinetic (PK) properties, making it a promising lead candidate for further structural optimization., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Mengyang Fan, Dawei Ma, Ke Ding, Hongjin Zhang and Suyun Jia are inventors of patent #CN2023/094038, which is related to the work reported in this paper. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
16. C-Phycocyanin improves the quality of goat oocytes after in vitro maturation and vitrification.
- Author
-
Wang XY, Gao Y, Liu HR, Wang T, Feng ML, Xue FR, Ding K, Yang Q, Jiang ZY, Sun D, Song CR, Zhang XJ, and Liang CG
- Subjects
- Animals, Female, Reactive Oxygen Species metabolism, Membrane Potential, Mitochondrial drug effects, Oocytes drug effects, In Vitro Oocyte Maturation Techniques veterinary, In Vitro Oocyte Maturation Techniques methods, Goats, Vitrification drug effects, Cryopreservation veterinary, Cryopreservation methods, Phycocyanin pharmacology
- Abstract
In vitro maturation (IVM) and cryopreservation of goat oocytes are important for establishing a valuable genetic bank for domesticated female animals and improving livestock reproductive efficiency. C-Phycocyanin (PC) is a Spirulina extract with antioxidant, antiinflammatory, and radical scavenging properties. However, whether PC has positive effect on goat oocytes IVM or developmental competence after vitrification is still unknown. In this study, we found that first polar body extrusion (n = 293), cumulus expansion index (n = 269), and parthenogenetic blastocyst formation (n = 281) were facilitated by adding 30 μg/mL PC to the oocyte maturation medium when compared with the control groups and that supplemented with 3, 10, 100 or 300 μg/mL PC (P < 0.05). Although PC supplementation did not affect spindle formation or chromosome alignment (n = 115), it facilitated or improved cortical granules migration (n = 46, P < 0.05), mitochondria distribution (n = 39, P < 0.05), and mitochondrial membrane potential (n = 46, P < 10
-4 ). Meanwhile, supplementation with 30 μg/mL PC in the maturation medium could significantly inhibit the reactive oxygen species accumulation (n = 65, P < 10-4 ), and cell apoptosis (n = 42, P < 0.05). In addition, PC increased the oocyte mRNA levels of GPX4 (P < 0.01), and decreased the mRNA and protein levels of BAX (P < 0.01). Next, we investigated the effect of PC supplementation in the vitrification solution on oocyte cryopreservation. When compared with the those equilibrate in the vitrification solution without PC, recovered oocytes in the 30 μg/mL PC group showed higher ratios of normal morphology (n = 85, P < 0.05), survival (n = 85, P < 0.05), first polar body extrusion (n = 62, P < 0.05), and parthenogenetic blastocyst formation (n = 107, P < 0.05). Meanwhile, PC supplementation of the vitrification solution increased oocyte mitochondrial membrane potential (n = 53, P < 0.05), decreased the reactive oxygen species accumulation (n = 73, P < 0.05), promoted mitochondria distribution (n = 58, P < 0.05), and inhibited apoptosis (n = 46, P < 10-3 ). Collectively, our findings suggest that PC improves goat oocyte IVM and vitrification by reducing oxidative stress and early apoptosis, which providing a novel strategy for livestock gamete preservation and utilization., Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported., (Copyright © 2024 Elsevier Inc. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
17. A Multi-Institutional Safety and Feasibility Study Exploring the Use of Hydrogel to Create Spatial Separation between the Pancreas and Duodenum in Patients with Pancreatic Cancer.
- Author
-
Narang AK, Hong TS, Ding K, Herman J, Meyer J, Thompson E, Bhutani MS, Krishnan K, Casey B, Shin EJ, and Koay EJ
- Subjects
- Humans, Male, Aged, Female, Middle Aged, Pancreas pathology, Pancreas radiation effects, Pancreas surgery, Adenocarcinoma radiotherapy, Adenocarcinoma pathology, Pancreatic Neoplasms radiotherapy, Pancreatic Neoplasms pathology, Feasibility Studies, Duodenum surgery, Duodenum radiation effects, Duodenum pathology, Hydrogels therapeutic use
- Abstract
Purpose: The administration of dose-escalated radiation for pancreatic adenocarcinoma remains challenging because of the proximity of dose-limiting stomach and bowel, particularly the duodenum for pancreatic head tumors. We explore whether endoscopic injection of a temporary, absorbable hydrogel into the pancreatico-duodenal (PD) groove is safe and feasible for the purpose of increasing spatial separation between pancreatic head tumors and the duodenum., Methods and Materials: Six patients with localized pancreatic adenocarcinoma underwent endoscopic injection of hydrogel into the PD groove. Safety was assessed based on the incidence of procedure-related adverse events resulting in a delay of radiation therapy initiation. Feasibility was defined as the ability to create spatial separation between the pancreas and duodenum, as assessed on simulation CT., Results: All 6 patients were able to undergo endoscopic injection of hydrogel into the PD groove. No device-related events were experienced at any point in follow-up. Presence of hydrogel in the PD groove was apparent on simulation CT in all 6 patients. Mean space created by the hydrogel was 7.7 mm +/- 2.4 mm. In 3 patients who underwent Whipple resection, presence of hydrogel in the PD groove was pathologically confirmed with no evidence of damage to the duodenum., Conclusions: Endoscopic injection of hydrogel into the PD groove is safe and feasible. Characterization of the dosimetric benefit that this technique may offer in the setting of dose-escalated radiation should also be pursued, as should the ability of such dosimetric benefit to translate into clinically improved tumor control., Competing Interests: Disclosures Amol Kumar Narang reports grants or contracts from Boston Scientific, Nanocan, and Flavocure. Theodore S. Hong reports grants or contracts from National Cancer Institute and SU2C. Received consulting fees from Synthetic Biologics, Boston Scientific, Merck, Inviata, GSK, and NextCure. Participated on a Data Safety Monitoring Board or Advisory Board for Novocure and Galera. Has stock or stock options from Panther Therapeutics. Kai Ding reports support for the present manuscript from Augmenix and Boston Scientific. Grants or contracts with NIH. Joseph Herman reports grants or contracts from 1440 Canopy Cancer Collective. Royalties or licenses from Springer Publishing. Consulting fees from Histosonics and Boston Scientific. Support for attending meetings and/or travel from Histosonics and 1440 Canopy Cancer Collective. Patents planned, issued or pending with Oncospace. Has stock or stock options from Histosonics. Jeffrey Meyer reports support for the present manuscript from Boston Scientific. Royalties or licenses from Springer and UpToDate. Manoop S. Bhutani reports support for the manuscript from Boston Scientific. Grants or contracts from Nanobiotix-Research Grant and Trisalis. Consulting fees from Oncosil Inc and Starpax Medical. Kumar Krishnan reports consulting fees from Boston Scientific and Olympus Medical. Euin Ji Shin reports consulting fees from Boston Scientific. Payment for expert testimony from US Dept of Justice. Eugene J, Koay reports support for the present manuscript from Dept of Defense and the NIH. Grants or contracts from Philips Health care, GE Health care, Stand up to Cancer, Project Purple, Elekta, and Dept of Defense. Royalties or licenses from Taylor and Francis LLC. Consulting fees from RenovoRx, AstraZeneca, and Augmenix. Payment or honoraria for lectures, presentations, speakers bureaus, manuscript writing or educational events from Apollo Cancer Hospitals in Chennai India, Bayer Health care, Philips Health care, and Aptitude Health. Patents planned, issued or pending for the design and fabrication of 3D printed oral stents for head and neck cancer. Leadership of fiduciary role in other board, society, committee or advocacy group, paid or unpaid with International Cholangiocarcinoma Research Network. Has stock or stock options with Quantum Aurea Capital., (Copyright © 2023 American Society for Radiation Oncology. Published by Elsevier Inc. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
18. Fluorescent Polylactic acid composite incorporating lignin-based carbon quantum dots for sustainable 4D printing applications.
- Author
-
Ren Z, Ding K, Zhou X, Ji T, Sun H, Chi X, Wei Y, and Xu M
- Subjects
- Fluorescence, Quantum Dots chemistry, Polyesters chemistry, Lignin chemistry, Printing, Three-Dimensional, Carbon chemistry
- Abstract
Fluorescent 4D printing materials, as innovative materials that combine fluorescent characteristics with 4D printing technology, have attracted widespread interest and research. In this study, green lignin-derived carbon quantum dots (CQDs) were used as the fluorescent module, and renewable poly(propylene carbonate) polyurethane (PPCU) was used for toughening. A new low-cost fluorescent polylactic acid (PLA) composite filament for 4D printing was developed using a simple melt extrusion method. The strength of the prepared composite was maintained at 32 MPa, while the elongation at break increased 8-fold (34 % increase), demonstrating excellent shape fixed ratio (∼99 %), recovery ratio (∼92 %), and rapid shape memory recovery speed. The presence of PPCU prevented fluorescence quenching of the CQDs in the PLA matrix, allowing the composite to emit bright green fluorescence under 365 nm ultraviolet light. The composite exhibited shear thinning behavior and had an ideal melt viscosity for 3D printing. The results obtained demonstrated the versatility of these easy-to-manufacture and low-cost filaments, opening up a novel and convenient method for the preparation of strong, tough, and multifunctional PLA materials, increasing their potential application value., Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest., (Copyright © 2024. Published by Elsevier B.V.)
- Published
- 2024
- Full Text
- View/download PDF
19. New polycyclic polyprenylated acylphloroglucinols with antidepressant activities from Hypericum perforatum L.
- Author
-
Pan XG, Li XX, Xia CY, Yin WF, Ding K, Zuo GY, Wang MN, Zhang WK, He J, and Xu JK
- Subjects
- Animals, Mice, Humans, Molecular Structure, Neuroprotective Agents pharmacology, Neuroprotective Agents chemistry, Neuroprotective Agents isolation & purification, Structure-Activity Relationship, Dose-Response Relationship, Drug, Male, Cell Line, Tumor, Polycyclic Compounds pharmacology, Polycyclic Compounds chemistry, Polycyclic Compounds isolation & purification, Corticosterone, Hindlimb Suspension, Hypericum chemistry, Antidepressive Agents pharmacology, Antidepressive Agents chemistry, Antidepressive Agents isolation & purification, Phloroglucinol pharmacology, Phloroglucinol chemistry, Phloroglucinol isolation & purification
- Abstract
Six new polycyclic polyprenylated acylphloroglucinols (PPAPs), hyperidiones A-F (1-6), were obtained from Hypericum perforatum L. Their structures were characterized via extensive spectroscopic analyses, the circular dichroism data of the in situ formed [Mo
2 (OCOCH3 )4 ] complexes, the nuclear magnetic resonance calculation with DP4+ probability analysis, and the calculated electronic circular dichroism (ECD) spectra. Compounds 1-6 are bicyclic polyprenylated acylphloroglucinols with a major bicyclo[3.3.1]nonane-2,4,9-trione skeleton. Notably, compound 1 is a rare PPAP with a hydroperoxy group, and a plausible biosynthetic pathway for 1 was proposed. Compounds 4 and 6 exhibited significant neuroprotective effects under 10 μM against corticosterone (CORT)-injured SH-SY5Y cells. Furthermore, compound 4 demonstrated a noteworthy antidepressant effect at the dose of 5 mg/kg in the tail suspension test (TST) of mice, which was equivalent to 5 mg/kg of fluoxetine. And it potentially exerted an antidepressant effect through the hypothalamic-pituitary-adrenal (HPA) axis., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
20. Research progress of industrial wastewater treatment technology based on solar interfacial adsorption coupled evaporation process.
- Author
-
Li J, Cao Y, Ding K, Ye J, Li F, Ma C, Lv P, Xu Y, and Shi L
- Abstract
Solar interface evaporation is an effective method for the treatment of water that has low energy consumption. Adsorption is recognized to be one of the most stable wastewater treatment methods and is widely used. Combining solar interface evaporation with adsorption provides a novel and low-cost approach for the efficient removal of heavy metals and organic pollutants from industrial wastewater. This paper reviews the characteristics and application of some common wastewater treatment methods. The photothermal conversion and the conceptual design of interface evaporation combined with adsorption are introduced and the photo-thermal conversion and adsorption methods are discussed. The study provides a summary of recent studies and advancements in interfacial evaporation-coupled adsorption materials, which include hydrogels, aerogels, and biomass materials for adsorption, and carbon materials for photothermal conversion. Finally, the current challenges encountered in industrial wastewater treatment are outlined and its prospects are discussed. The aim of this review is to explore a wide range of possibilities with the interfacial evaporation-coupled adsorption method and propose a new low-cost and high-efficiency method for industrial wastewater treatment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
21. Global distribution, cross-species transmission, and receptor binding of canine parvovirus-2: Risks and implications for humans.
- Author
-
Chen S, Shang K, Chen J, Yu Z, Wei Y, He L, and Ding K
- Subjects
- Animals, Dogs, Humans, Parvoviridae Infections veterinary, Parvoviridae Infections transmission, Cats, Capsid Proteins metabolism, Capsid Proteins genetics, Zoonoses virology, Zoonoses transmission, Receptors, Transferrin metabolism, Receptors, Transferrin genetics, Parvovirus, Canine genetics
- Abstract
For canine parvovirus -2 (CPV-2), a zoonotic virus capable of cross-species transmission in animals, the amino acid changes of capsid protein VP2 are key factors when binding to other species' transferrin receptors (TfR). CPV-2 variants can spread from felines and canines, for example, to Carnivora, Artiodactyla, and Pholidota species, and CPV-2c variants are essential to spread from Carnivora to Artiodactyla and Pholidota species in particular. In our study, a CPV-2a variant maintained a relatively stable trend, and the proportion of CPV-2c gradually rose from 1980 to 2021. The VP2 amino acid sequence analysis showed that five amino acid mutations at 426E/D, 305H/D, and 297S may be necessary for the virus to bind to different host receptors. Meanwhile, receptor-binding loop regions and amino acid sites 87 L, 93 N, 232I, and 305Y were associated with CPV-2 cross-species transmission. The homology of TfRs in different hosts infected with CPV-2 ranged from 77.2 % to 99.0 %, and from pig to feline, canine, and humans was 80.7 %, 80.4 %, and 77.2 %, respectively. The amino acid residues of TfRs involved in the viral binding in those hosts are highly conserved, which suggests that CPV-2 may be capable of pig-to-human transmission. Our analysis of the origin, evolutionary trend, cross-species transmission dynamics, and genetic characteristics of CPV-2 when binding to host receptors provides a theoretical basis for further research on CPV-2's mechanism of cross-species transmission and for establishing an early warning and monitoring mechanism for the possible threat of CPV-2 to animal-human public security., Competing Interests: Declaration of competing interest The authors declare that they have no completing interests., (Copyright © 2023. Published by Elsevier B.V.)
- Published
- 2024
- Full Text
- View/download PDF
22. Protective effect of selenomethionine on rabbit testicular injury induced by Aflatoxin B1.
- Author
-
Zhang Z, Zhang Z, Liu S, Wei S, Wei L, Zhu X, Ding K, and Liu Y
- Subjects
- Animals, Male, Rabbits, Testosterone blood, Protective Agents pharmacology, Testicular Diseases prevention & control, Testicular Diseases chemically induced, Oxidative Stress drug effects, Luteinizing Hormone blood, Apoptosis drug effects, Aflatoxin B1 toxicity, Selenomethionine pharmacology, Testis drug effects
- Abstract
The aim of this study was to investigate the alleviating effect of selenomethionine (SeMet) on aflatoxin B1 (AFB1)-induced testicular injury in rabbits. Twenty-five 90-d-old rabbits were randomly divided into 5 groups (the control group, the AFB1 group, the 0.2 mg/kg SeMet + AFB1 group, the 0.4 mg/kg SeMet + AFB1 group and the 0.6 mg/kg SeMet + AFB1 group). After 1 d of the experiment, the SeMet-treated groups were fed 0.2 mg/kg SeMet, 0.4 mg/kg SeMet, or 0.6 mg/kg SeMet daily, and the remaining two groups were fed a normal diet for 30 d. On Day 31, all rabbits in the model group and the three treatment groups were fed 0.5 mg/kg AFB1 for 21 d. The levels of testosterone (T), luteinizing hormone (LH) and follicle stimulating hormone (FSH) in rabbit plasma were detected. Rabbit semen was collected, and its quality was evaluated. Pathological changes in rabbit testes were observed by hematoxylin-eosin (HE) staining. The expression of related proteins in testicular tissue was detected by immunohistochemistry, immunofluorescence and western blot (WB) analysis. Enzyme-linked immunosorbent assays (ELISAs) were used to detect oxidative stress-related indices and inflammatory factors in testicular tissue. The results showed that AFB1 can induce oxidative stress and inflammation to activate the p38/MSK/NF-κB signalling pathway, mediate apoptosis, inhibit the proliferation and differentiation of testicular cells, destroy the integrity of the blood-testis barrier (BTB) and the normal structure of the testis, and reduce the content of sex hormones and semen quality. SeMet pretreatment significantly alleviated testicular injury oxidative stress, and the inflammatory response in rabbits. Thus, we demonstrated that SeMet restores AFB1-induced testicular toxicity by inhibiting the p38/MSK/NF-κB signalling pathway. In addition, in this study, 0.4 mg/kg SeMet had the most impactful effect., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier B.V.)
- Published
- 2024
- Full Text
- View/download PDF
23. Antibacterial polyurethane foams with quaternized-chitosan as a chain extender for nasal packing and hemostasis.
- Author
-
Ding K, Cong W, Liu Y, Song C, Mi H, Liu C, Ma Y, and Shen C
- Subjects
- Rabbits, Animals, Hemostasis drug effects, Staphylococcus aureus drug effects, Hemostatics chemistry, Hemostatics pharmacology, Bandages, Escherichia coli drug effects, Male, Polyurethanes chemistry, Polyurethanes pharmacology, Chitosan chemistry, Chitosan pharmacology, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents chemistry
- Abstract
Endoscopic surgery is an effective and common clinical practice for chronic sinusitis. Nasal packing materials are applied in nasal surgery to prevent hemorrhage and promote wound healing. In this study, a degradable polyurethane foam dressing is successfully developed as a promising nasal packing material with good biocompatibility and antibacterial capability. Specifically, quaternized chitosan (QCS) serves as the crosslinker instead of polyols to offer polyurethane foam (PUF-QCS) antibacterial capability. The PUF-QCS2.0 % (with 2.0 wt% QCS) exhibits satisfactory liquid absorption capacity (19.4 g/g), high compressive strengths at both wet (14.5 kPa) and dry states (7.7 kPa), and a good degradation rate (8.3 %) within 7 days. Meanwhile, PUF-QCS2.0 % retains long-term antibacterial activity for 7 days and kills 97.3 % of S. aureus and 91.8 % of E. coli within 6 hours in antibacterial testing. Furthermore, PUF-QCS2.0 % demonstrates a positive hemostatic response in the rabbit nasal septum mucosa trauma model by reducing hemostatic time over 50.0 % and decreasing blood loss up to 76.1 % compared to the commercial PVA nasal packing sponge. Importantly, PUF-QCS also exhibits a significant antibacterial activity in nasal cavity. This nasal packing material has advantages in post-surgery bleeding control and infection prevention. STATEMENT OF SIGNIFICANCE: The performance of a nasal packing sponge requires good mechanical properties, fast and high liquid absorption rate, effective degradability and strong antibacterial activity. These features are helpful for improving the postoperative recovery and patient healing. However, integrating these into a single polyurethane foam is a challenge. In this study, quaternized chitosan (QCS) is synthesized and used as a chain extender and antibacterial agent in preparing a degradable polyurethane foam (PUF-QCS) dressing. PUF-QCS undergoes partial degradation and exhibits effective broad-spectrum antibacterial activity in 7 days. The reduction of postoperative bleeding and infection observed in the animal experiment further demonstrates that the PUF-QCS developed here outperforms the existing commercial nasal packing materials., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
24. Climate change intensifies algal biomass resurgence in eutrophic Lake Taihu, China.
- Author
-
Pan T, Cui C, Qin B, Ding K, and Zhou J
- Subjects
- Biomass, Environmental Monitoring, Phytoplankton, Eutrophication, China, Phosphates, Nitrogen, Phosphorus analysis, Lakes, Climate Change
- Abstract
Climate change can significantly alter phytoplankton growth and proliferation, which would counteract restoration efforts to control algal blooms. However, the knowledge is limited about the quantitative evaluation of the causal effect of algal biomass resurgence in large shallow lakes where there is no significant improvement after long term lake restoration. Here, a bucket process-based phytoplankton dynamic model is developed to quantify the contributions of climate change and nutrients concentration changes to phytoplankton biomass resurgence after 2014 in hypereutrophic Lake Taihu, China. Compared to 2008-2014, the mean water temperature (WT) and the mean phosphate are higher, the mean photosynthetically active radiation (PAR), the mean total suspended solids (TSS), and the mean dissolved inorganic nitrogen (DIN) are lower, during 2015-2020. Their contribution to algal biomass resurgence during 2015-2020 is WT (+58.7 %), PAR (-2.6 %), TSS (+23.2 %), DIN (-22.1 %) and phosphate (+42.7 %), respectively. Climate change (WT, PAR, and TSS), which contributed +64.9 % to the phytoplankton biomass resurgence, underscores the urgent need to continuously take more effective measures to reduce nutrient emissions to offset the effects of climate change in Lake Taihu and in other eutrophic lakes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationship that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
25. Role of transcriptional cofactors in cardiovascular diseases.
- Author
-
Mao S, Song C, Huang H, Nie Y, Ding K, Cui J, Tian J, and Tang H
- Subjects
- Humans, Transcription Factors genetics, Transcription Factors metabolism, Transcription, Genetic, Nuclear Proteins metabolism, Gene Expression Regulation, RNA Polymerase II metabolism, Bromodomain Containing Proteins, Cell Cycle Proteins metabolism, Cardiovascular Diseases genetics, Sirtuins metabolism
- Abstract
Cardiovascular disease is a main cause of mortality in the world and the highest incidence of all diseases. However, the mechanism of the pathogenesis of cardiovascular disease is still unclear, and we need to continue to explore its mechanism of action. The occurrence and development of cardiovascular disease is significantly associated with genetic abnormalities, and gene expression is affected by transcriptional regulation. In this complex process, the protein-protein interaction promotes the RNA polymerase II to the initiation site. And in this process of transcriptional regulation, transcriptional cofactors are responsible for passing cues from enhancers to promoters and promoting the binding of RNA polymerases to promoters, so transcription cofactors playing a key role in gene expression regulation. There is growing evidence that transcriptional cofactors play a critical role in cardiovascular disease. Transcriptional cofactors can promote or inhibit transcription by affecting the function of transcription factors. It can affect the initiation and elongation process of transcription by forming complexes with transcription factors, which are important for the stabilization of DNA rings. It can also act as a protein that interacts with other proteins to affect the expression of other genes. Therefore, the aim of this overview is to summarize the effect of some transcriptional cofactors such as BRD4, EP300, MED1, EZH2, YAP, SIRT6 in cardiovascular disease and to provide a promising therapeutic strategy for the treatment of cardiovascular disease., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
26. Tracing nitrate origins and transformation processes in groundwater of the Hohhot Basin's Piedmont strong runoff zone through dual isotopes and hydro-chemical analysis.
- Author
-
Ding K, Zhang Y, Zhang H, Yu C, Li X, Zhang M, Zhang Z, and Yang Y
- Abstract
Nitrate, which poses a serious threat to the drinking water supply, is one of the most prevalent anthropogenic groundwater contaminants worldwide. With the development of the chemical industry, the nitrate pollution of groundwater in the Piedmont strong runoff zone of the Hohhot Basin, which is the main groundwater extraction area, is becoming increasingly severe. The special hydrogeological and complex pollution conditions in the study area make it difficult to identify nitrate sources and transformation processes. In order to identify the results more accurately, this study combined water chemistry, multivariate statistical analysis and isotope tracer methods to determine the sources and transformation processes of nitrate in the study area. The results showed that the groundwater in the eastern part of the study area (ESA) was clearly affected by anthropogenic activities, and its nitrate was mainly from nitrification of ammonia in industrial wastewater, nitrate in industrial wastewater (the sum of the two contributions was 62.2 %), and nitrate in manure (20.5 %). The hydrogeochemical characteristics of groundwater in the western part of the study area (WSA) are the same as those of natural groundwater in the Piedmont strong-runoff zone. The nitrate in groundwater in the WSA was mainly derived from soil nitrogen (63.8 %) and ammonia fertilizer (28.8 %). Nitrification and denitrification occurred only locally in the aquifer of the study area and were more pronounced in the ESA. Meanwhile, the transformation processes of nitrate in groundwater in the ESA and WSA was significantly influenced by contamination with chlorinated hydrocarbon volatile organic compounds and hydrogeological conditions, respectively. These findings provide a scientific basis for the development of groundwater pollution prevention measures in the study area and guide the traceability of nitrate in groundwater in areas with similar hydrogeological and pollution conditions., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Yilong Zhang reports financial support was provided by National Key Research and Development Program of China., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
27. Integrated assessment of the pollution and risk of heavy metals in soils near chemical industry parks along the middle Yangtze River.
- Author
-
Xu D, Wang Z, Tan X, Xu H, Zhu D, Shen R, Ding K, Li H, Xiang L, and Yang Z
- Subjects
- Soil, Cadmium analysis, Rivers, Chemical Industry, Environmental Monitoring, Risk Assessment, Nickel analysis, China, Soil Pollutants analysis, Metals, Heavy analysis, Arsenic analysis, Mercury analysis
- Abstract
Industrialization in riparian areas of critical rivers has caused significant environmental and health impacts. Taking eight industrial parks along the middle Yangtze River as examples, this study proposes a multiple-criteria approach to investigate soil heavy metal pollution and associated ecological and health risks posed by industrial activities. Aiming at seven heavy metals, the results show that nickel (Ni), cadmium (Cd), and copper (Cu) exhibited the most significant accumulation above background levels. The comprehensive findings from Pearson correlation analysis, cluster analysis, principal component analysis, and industrial investigation uncover the primary sources of Cd, arsenic (As), mercury (Hg), and lead (Pb) to be chemical processing, while Ni and chromium (Cr) are predominantly derived from mechanical and electrical equipment manufacturing. In contrast, Cu exhibits a broad range of origins across various industrial processes. Soil heavy metals can cause serious ecological and carcinogenic health risks, of which Cd and Hg contribute to >70 % of the total ecological risk, and As contributes over 80 % of the total health risk. This study highlights the importance of employing multiple mathematical and statistical models in determining and evaluating environmental hazards, and may aid in planning the environmental remediation engineering and optimizing the industry standards., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
28. The safety and efficacy of NOACs versus LMWH for thromboprophylaxis after THA or TKA: A systemic review and meta-analysis.
- Author
-
Ding K, Yan W, Zhang Y, Li J, Li C, and Liang C
- Abstract
The differences in the safety and efficacy of anticoagulation between different types of new oral anticoagulants(NOACs) and low molecular weight heparin(LMWH) are still controversial. The main purposes of this study were to analyze safety and efficacy of NOACs versus LMWH for thromboprophylaxis, and perform subgroup analyses stratified by individual NOACs and different populations after total hip arthroplasty (THA) or total knee arthroplasty (TKA). Literature search was performed in PubMed, EMBASE, Cochrane Library, CNKI and Wanfang databases until June 31, 2022. This systematic review and meta-analysis included 46 randomized controlled trials (RCT) with 39, 924 patients. We evaluated the safety and efficacy of thromboprophylaxis between LMWH and NOACs. NOACs were more effective in reducing deep vein thrombosis (DVT) (RR0.59; 95%CI 0.49-0.71) and adverse events (RR: 0.96; 95%CI: 0.93-0.99) than LMWH. The subgroup analyses for different anticoagulants revealed that rivaroxaban (RR:0.49; 95%CI:0.36-0.66), apixaban (RR: 0.54; 95%CI: 0.36-0.81) and edoxaban (RR:0.49; 95%CI: 0.32-0.75) have the lower risk of DVT than LMWH. Apixaban (RR:0.89; 95%CI: 0.80-1.00) had superior prevention of bleeding to LMWH. Edoxaban exhibited a lower risk of VTE (RR: 0.46; 95%CI: 0.33-0.65), advantage events (RR: 0.87; 95%CI: 0.82-0.93), and drug-related adverse events (DRAEs) (RR: 0.64; 95%CI: 0.53-0.76) than LMWH. East Asian population was superior to western population for preventing DVT, advantage events, and DRAE using NOACs. In conclusion, NOACs are more effective than LMWH at preventing DVT and adverse events after arthroplasty. Apixaban has lower bleeding than LMWH, and East Asian populations may benefit more than western population from NOACs., Competing Interests: Declaration of competing interest The authors declare no competing interests., (Copyright © 2024 Asian Surgical Association and Taiwan Robotic Surgery Association. Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
29. Data-driven assessment of soil total nitrogen on the Qinghai-Tibet Plateau.
- Author
-
Zhao J, Jiang P, Shen T, Zhang R, Zhang D, Zhang N, Ting N, Ding K, Yang B, Tan C, and Yu Z
- Abstract
The investigation of soil total nitrogen (STN) holds significant importance in the preservation and sustainability of Earth's ecosystems. The Qinghai-Tibet Plateau (QTP), renowned as the world's most expansive plateau and characterized by its exceptionally delicate ecosystem, demands an in-depth exploration of its STN content. In this study, we use a machine learning approach to extrapolate point-scale measured STN stocks to the entire QTP and calculated STN storage from 0 to 2 m. Our results show that the XGB algorithm performs well in modeling STN despite variations in simulation accuracy for specific depth ranges. The spatial distribution of STN across the QTP exhibits pronounced heterogeneity, especially for the 0-50 cm soil layer, with relatively higher STN stocks in the southeast and lower stocks in the northwest of QTP. The vertical distribution reveals a gradual decrease in STN storage with increasing depth. The 0-50 cm soil layer holds the highest STN stocks, averaging around 0.78 kg/m
2 , which is almost the sum of STN stocks in the 50-100 cm and 100-200 cm soil layers. Meanwhile, the STN stocks are smaller in permafrost zone than that in non-permafrost zone. We also investigate the impact factors that control the spatiotemporal distribution of STN. It indicates that vegetation, precipitation, temperature, and elevation are the major factors for STN distribution, while physical properties of the soil have a relatively smaller impact. These findings are crucial for understanding the distribution and evolution of STN on the QTP., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
30. A case of Kaposi sarcoma arising from giant condyloma acuminatum.
- Author
-
Ge Y, Yu M, Si Z, and Ding K
- Subjects
- Humans, Buschke-Lowenstein Tumor surgery, Sarcoma, Kaposi etiology, Sarcoma, Kaposi surgery, Anus Neoplasms, Condylomata Acuminata surgery
- Abstract
Competing Interests: Declaration of competing interest None.
- Published
- 2024
- Full Text
- View/download PDF
31. Stereo-electroencephalographic seizure localization in patients with mesial temporal sclerosis: A single center experience.
- Author
-
Zhang B, Podkorytova I, Hays R, Perven G, Agostini M, Harvey J, Zepeda R, Alick-Lindstrom S, Dieppa M, Doyle A, Das R, Lega B, and Ding K
- Abstract
Objective: Epilepsy patients with mesial temporal sclerosis (MTS) on imaging who are drug-resistant usually undergo epilepsy surgery without previous invasive evaluation. However, up to one-third of patients are not seizure-free after surgery. Prior studies have identified risk factors for surgical failure, but it is unclear if they are associated with bilateral or discordant seizure onset., Methods: In this retrospective case series, we identified 17 epilepsy patients who had MRI-confirmed MTS but received invasive stereo-EEG (SEEG) evaluation before definitive intervention. We analyzed their presurgical risk factors in relation to SEEG seizure onset localization and MRI/SEEG concordance., Results: SEEG ictal onset was concordant with MTS localization (i.e. seizures started only from the hippocampus with MTS) in 5 out of 13 patients with unilateral MTS (UMTS) and in 3 out of 4 patients with bilateral MTS.No statistically significant association regarding concordance of SEEG ictal onset and MTS location was found in patients with such risk factors as a history of non-mesial temporal aura, frequent focal to bilateral tonic-clonic seizures, prior viral brain infection, or family history of epilepsy. Nine out of 13 UMTS patients had resective surgery only, 5 out of 9 (56 %) have Engel class I outcome at most recent follow-up (median 46.5 months, range 22-91 months). In Engel class I cohort, the SEEG ictal onset was concordant with MTS location in 3 out of 5 patients, and 2 patients had ipsilateral temporal neocortical ictal onset., Conclusions: Our findings suggest that patients with MTS might have discordant SEEG ictal onset (in 61.5% patients with UMTS in presented cohort), which may explain poor surgical outcome after destructive surgery in these cases., Significance: Although no statistically significant association was found in this under-powered study, these findings could be potentially valuable for future meta -analyses., (© 2024 International Federation of Clinical Neurophysiology. Published by Elsevier B.V.)
- Published
- 2024
- Full Text
- View/download PDF
32. Discovery of a potent and selective covalent threonine tyrosine kinase (TTK) inhibitor.
- Author
-
Sun Y, Chen Z, Liu G, Chen X, Shi Z, Feng H, Yu L, Li G, Ding K, Huang H, Zhang Z, and Xu S
- Subjects
- Humans, Cell Line, Tumor, Protein Serine-Threonine Kinases, Protein-Tyrosine Kinases antagonists & inhibitors, Cell Cycle Proteins, Threonine, Tyrosine Kinase Inhibitors chemistry, Tyrosine Kinase Inhibitors pharmacology
- Abstract
Threonine tyrosine kinase (TTK) is a critical component of the spindle assembly checkpoint and plays a pivotal role in mitosis. TTK has been identified as a potential therapeutic target for human cancers. Here, we describe our design, synthesis and evaluation of a class of covalent TTK inhibitors, exemplified by 16 (SYL1073). Compound 16 potently inhibits TTK kinase with an IC
50 of 0.016 μM and displays improved selectivity in a panel of kinases. Mass spectrometry analysis reveals that 16 covalently binds to the C604 cysteine residue in the hinge region of the TTK kinase domain. Furthermore, 16 achieves strong potency in inhibiting the growth of various human cancer cell lines, outperforming its relative reversible inhibitor, and eliciting robust downstream effects. Taken together, compound 16 provides a valuable lead compound for further optimization toward the development of drug for treatment of human cancers., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
33. Neuroprotective effects of cordycepin inhibit glutamate-induced apoptosis in hippocampal neurons.
- Author
-
Sun H, Wei S, Gong Y, Ding K, Tang S, Sun W, Yuan C, Huang L, Liu Z, Chen C, and Yao L
- Subjects
- Glutamic Acid toxicity, Glutamic Acid metabolism, Acetylcholinesterase metabolism, Acetylcholinesterase pharmacology, Reactive Oxygen Species metabolism, Calcium metabolism, Apoptosis, Deoxyadenosines pharmacology, Deoxyadenosines metabolism, Hippocampus metabolism, Neurons metabolism, Glutathione metabolism, Neuroprotective Agents pharmacology
- Abstract
Glutamate is a neurotransmitter that can cause excitatory neurotoxicity when its extracellular concentration is too high, leading to disrupted calcium balance and increased production of reactive oxygen species (ROS). Cordycepin, a nucleoside adenosine derivative, has been shown to protect against excitatory neurotoxicity induced by glutamate. To investigate its potential neuroprotective effects, the present study employed fluorescence detection and spectrophotometry techniques to analyze primary hippocampal-cultured neurons. The results showed that glutamate toxicity reduced hippocampal neuron viability, increased ROS production, and increased intracellular calcium levels. Additionally, glutamate-induced cytotoxicity activated acetylcholinesterase and decreased glutathione levels. However, cordycepin inhibited glutamate-induced cell death, improved cell viability, reduced ROS production, and lowered Ca
2+ levels. It also inhibited acetylcholinesterase activation and increased glutathione levels. This study suggests that cordycepin can protect against glutamate-induced neuronal injury in cell models, and this effect was inhibited by adenosine A1 receptor blockers, indicating that its neuroprotective effect is achieved through activation of the adenosine A1 receptor., Competing Interests: Declarations of interest The authors report no biomedical financial interests or potential conflicts of interest., (Copyright © 2024 The Author(s). Published by Elsevier Inc. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
34. Prophylactic hyperthermic intraperitoneal chemotherapy in T4 colorectal cancer: Can it improve the oncologic prognosis? - A propensity score matching study.
- Author
-
Zheng Y, Zhang J, Chen C, Gong Z, Wang Z, Deng Q, Yu S, Hu Y, Liu Y, Cao H, Xiao Q, Wang J, Ding K, and Sun L
- Subjects
- Humans, Hyperthermic Intraperitoneal Chemotherapy, Combined Modality Therapy, Retrospective Studies, Cohort Studies, Propensity Score, Prognosis, Cytoreduction Surgical Procedures, Survival Rate, Colorectal Neoplasms pathology, Hyperthermia, Induced
- Abstract
Background: Some studies show that cytoreductive surgery with hyperthermic intraperitoneal chemotherapy (HIPEC) may improve overall survival and is a possible curative treatment for selected colorectal cancer (CRC) patients with restricted peritoneal metastasis (PM). The value of HIPEC in preventing PM of CRC is still controversial., Materials and Methods: In this retrospective propensity score matching (PSM) cohort study, all patients with cT4N0-2M0 undergoing treatment at a single institution in China (2014-2018) were reviewed. The 3-year disease-free survival (DFS) was set as the primary outcome, and the 3-year PM rate was also analyzed., Results: 220 patients were included in this study for analysis. After 1:3 PSM: HIPEC (n = 45) and No HIPEC (n = 135). Through analysis, it was found that prophylactic HIPEC correlated to better DFS [hazard ratio (HR) 0.43, 95 % confidence interval (CI) 0.19-0.95; p = 0.037], and N2 stage correlated to worse DFS [HR 1.97, 95 % CI 1.09-3.56; p = 0.025]. For laparoscopic surgery subgroup analyses, 3-year PM rate of patients with laparoscopic surgery was 13.8 % in No HIPEC group, and 2.6 % in HIPEC group (p = 0.070). Besides, no post-operative death occurred, the anastomotic leakage rate was 2.2 % in HIPEC group and 0.7 % in the control group (p = 0.439)., Conclusions: Prophylactic HIPEC may improve the prognosis in patients with cT4N0-1M0 CRC, but not in cT4N2M0 CRC, and it does not significantly increase surgery-related complications. Laparoscopic surgery followed by HIPEC for T4 stage CRC may not increase risk of PM., (© 2024 Published by Elsevier Ltd.)
- Published
- 2024
- Full Text
- View/download PDF
35. Continuous cropping disorders of eggplants (Solanum melongena L.) and tomatoes (Solanum lycopersicum L.) in suburban agriculture: Microbial structure and assembly processes.
- Author
-
Xiao Z, Lu C, Wu Z, Li X, Ding K, Zhu Z, Han R, Zhao J, Ge T, Li G, and Zhu YG
- Subjects
- Soil Microbiology, Agriculture, Bacteria genetics, Soil chemistry, Rhizosphere, Solanum lycopersicum, Solanum melongena
- Abstract
Deciphering the intricate relationships between microorganisms and plants remains a formidable challenge in plant microbial ecology, an area that holds promise for optimizing microbial interventions to enhance stress resilience and agricultural yields. In our investigation, we procured samples during 2019 and 2022 from a suburban agricultural greenhouse. Our study delineated the composition of bacterial and fungal communities across various ecological niches-namely, the rhizosphere soil, bulk soil, and phyllosphere of healthy, Ralstonia solanacearum-infected, and dead eggplants and tomatoes. The structure and composition of both fungal and bacterial communities change significantly under the influence of the host genotype across all samples. In the tomato or eggplant groups, bacterial wilt exerts a more pronounced impact on the bacterial community than on the fungal community. We speculate that the rhizosphere of healthy eggplants and tomatoes harbored more antibiotic-producing (e.g., Amycolatopsis and Penicillium) and biocontrol (e.g., Bacillus) strains, which can lead to have lower absolute abundance of R. solanacearum. In the context of R. solanacearum invasion, deterministic processes were responsible for shaping 70.67 % and 80.63 % of the bacterial community assembly in the rhizosphere of eggplants and tomatoes, respectively. Deterministic processes dominated the assembly of fungal communities in the rhizosphere of R. solanacearum-infected eggplants, whereas the opposite was true in the tomatoes. Homogeneous selection emerged as the predominant force governing the bacterial community assembly in the rhizospheres of R. solanacearum-infected eggplants and tomatoes. The bacterial co-occurrence networks in healthy rhizosphere soil were characterized by reduced vulnerability and enhanced stability (i.e., robustness index) and complexity (i.e., cohesion index), compared to their infected counterparts. In summary, complex microbial networks in rhizosphere soils are more resistant to invasion by soil-borne pathogens. The dynamics of bacterial interactions and community assembly processes are pivotal for effective microbiome management and offer predictive insights into the ecological ramifications of R. solanacearum invasions., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)
- Published
- 2024
- Full Text
- View/download PDF
36. Learning reduced-order models for cardiovascular simulations with graph neural networks.
- Author
-
Pegolotti L, Pfaller MR, Rubio NL, Ding K, Brugarolas Brufau R, Darve E, and Marsden AL
- Subjects
- Computer Simulation, Hemodynamics, Models, Cardiovascular, Neural Networks, Computer, Cardiovascular System
- Abstract
Reduced-order models based on physics are a popular choice in cardiovascular modeling due to their efficiency, but they may experience loss in accuracy when working with anatomies that contain numerous junctions or pathological conditions. We develop one-dimensional reduced-order models that simulate blood flow dynamics using a graph neural network trained on three-dimensional hemodynamic simulation data. Given the initial condition of the system, the network iteratively predicts the pressure and flow rate at the vessel centerline nodes. Our numerical results demonstrate the accuracy and generalizability of our method in physiological geometries comprising a variety of anatomies and boundary conditions. Our findings demonstrate that our approach can achieve errors below 3% for pressure and flow rate, provided there is adequate training data. As a result, our method exhibits superior performance compared to physics-based one-dimensional models while maintaining high efficiency at inference time., Competing Interests: Declaration of competing interest None declared., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
37. SeMet alleviates AFB 1 -induced oxidative stress and apoptosis in rabbit kidney by regulating Nrf2//Keap1/NQO1 and PI3K/AKT signaling pathways.
- Author
-
Liu Y, Wang J, Chang Z, Li S, Zhang Z, Liu S, Wang S, Wei L, Lv Q, Ding K, and Zhang Z
- Subjects
- Animals, Rabbits, Antioxidants pharmacology, Antioxidants metabolism, Apoptosis drug effects, Kelch-Like ECH-Associated Protein 1 metabolism, NF-E2-Related Factor 2 metabolism, Phosphatidylinositol 3-Kinases metabolism, Proto-Oncogene Proteins c-akt metabolism, Signal Transduction, Aflatoxin B1 toxicity, NAD(P)H Dehydrogenase (Quinone) drug effects, NAD(P)H Dehydrogenase (Quinone) metabolism, Kidney pathology, Oxidative Stress drug effects, Selenomethionine pharmacology
- Abstract
The purpose of this study was to explore the protective effect of SeMet on renal injury induced by AFB
1 in rabbits and its molecular mechanism. Forty rabbits of 35 days old were randomly divided into control group, AFB1 group (0.3 mg AFB1 /kg b.w), 0.2 mg/kg Se + AFB1 group (0.3 mg AFB1 /kg b.w + 0.2 mg SeMet/kg feed) and 0.4 mg/kg Se + AFB1 group (0.3 mg AFB1 /kg b.w + 0.4 mg SeMet/kg feed). The SeMet treatment group was fed different doses of SeMet diets every day for 21 days. On the 17-21 day, the AFB1 treatment group, the 0.2 mg/kg Se + AFB1 group and the 0.4 mg/kg Se + AFB1 group were administered 0.3 mg AFB1 /kg b.w by gavage (dissolved in 0.5 ml olive oil) respectively. The results showed that AFB1 poisoning resulted in the changes of renal structure, the increase of renal coefficient and serum biochemical indexes, the ascent of ROS and MDA levels, the descent of antioxidant enzyme activity, and the significant down-regulation of Nrf2, HO-1 and NQO1. Besides, AFB1 poisoning increased the number of renal apoptotic cells, rised the levels of PTEN, Bax, Caspase-3 and Caspase-9, and decreased the levels of PI3K, AKT, p-AKT and Bcl-2. In summary, SeMet was added to alleviate the oxidative stress injury and apoptosis of kidney induced by AFB1 , and the effect of 0.2 mg/kg Se + AFB1 is better than 0.4 mg/kg Se + AFB1 ., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2024
- Full Text
- View/download PDF
38. Design of sustainable 3D printable polylactic acid composites with high lignin content.
- Author
-
Ren Z, Zhou X, Ding K, Ji T, Sun H, Chi X, Wei Y, Xu M, Cai L, and Xia C
- Subjects
- Polymers, Plasticizers, Lignin, Polyesters
- Abstract
In this study, we report the development of a sustainable polymer system with 50 wt% lignin content, suitable for additive manufacturing and high value-added utilization of lignin. The plasticized polylactic acid (PLA) was incorporated with lignin to develop the bendable and malleable green composites with excellent 3D printing adaptability. The biocomposites exhibit increases of 765.54 % and 125.27 % in both elongation and toughness, respectively. The plasticizer enhances the dispersion of lignin and the molecular mobility of the PLA chains. The good dispersion of lignin particles within the structure and the reduction of chemical cross-linking promote the local relaxation of the polymer chains. The good local relaxation of the polymer chains and the high flexibility allow to obtain a better integration between the printed layers with good printability. This research demonstrates the promising potential of this composite system for sustainable manufacturing and provides insights into novel material design for high-value applications of lignin., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
39. 4D printing light-driven actuator with lignin photothermal conversion module.
- Author
-
Ren Z, Ding K, Zhou X, Ji T, Sun H, Chi X, and Xu M
- Subjects
- Infrared Rays, Polyesters, Printing, Three-Dimensional, Lignin, Elastomers
- Abstract
Light-responsive shape memory polymers are attractive as they can be activated through remote and spatially-controlled light. In this work, 4D printing of poly(lactic acid) (PLA) composites with a near-infrared light-responsive was achieved by using the simple melt blending method and adding 3 wt% of lignin. Lignin with a conjugated structure was used as the photothermal conversion module. The composites exhibited significant photothermal effects under near-infrared (808 nm) laser irradiation, and the laser irradiation was also effective in initiating and controlling the shape memory. The structure of lignin can be improved by the action of dicumyl peroxide (DCP) to enhance the interfacial adhesion between polyamide elastomer (PAE) and polylactic acid (PLA), reduce the size of dispersed phases, and serve as an effective rheological modifier to exhibit the ideal melt viscosity required for 3D printing of composites. The good mechanical, thermal stability, and rheological properties provide assurance for the 4D printing of composites. This research provides an environmentally friendly and practical method for creating composites that have the potential to serve as ideal actuator components in a range of applications., Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
40. A miniaturized hydrogen flame ionization detector based on integrated nozzle assembly and embedded sealing structure.
- Author
-
Li S, Geng X, Ding K, Gao Y, and Guan Y
- Abstract
A modified miniaturized hydrogen flame ionization detector (m-FID) was developed and evaluated. An integrated nozzle assembly was constructed to solve the gas leakage caused by adhesive crack during repeated high-low temperature processes or vibration. An embedded sealing structure was designed to realize the face sealing, thus improving the sealing stability and reliability of the m-FID. Polyimide was employed as seal and insulation material to ensure the detector can be used at 300 °C for a long term. The hydrogen and air consumption of the m-FID was 12 mL/min and 110 mL/min, which is about 1/3 of the FID gas consumption of commercial laboratory instruments. The limit of detection (LOD) for n-hexadecane was 3.2 × 10
-12 g/s, with a linear response range of nearly 5 orders of magnitude. Finally, it was installed onto an on-site gas chromatograph to detect drug samples with wide boiling point range from room temperature up to 535 °C., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2023
- Full Text
- View/download PDF
41. Neutron reflection and scattering in characterising peptide assemblies.
- Author
-
Hu X, Liao M, Ding K, Wang J, Xu H, Tao K, Zhou F, and Lu JR
- Subjects
- Neutrons, Scattering, Small Angle, Peptides chemistry, Proteins chemistry
- Abstract
Self-assemblies of de novo designed short peptides at interface and in bulk solution provide potential platforms for developing applications in many medical and technological areas. However, characterising how bioinspired supramolecular nanostructures evolve with dynamic self-assembling processes and respond to different stimuli remains challenging. Neutron scattering technologies including small angle neutron scattering (SANS) and neutron reflection (NR) can be advantageous and complementary to other state-of-the-art techniques in tracing structural changes under different conditions. With more neutron sources now available, SANS and NR are becoming increasingly popular in studying self-assembling processes of diverse peptide and protein systems, but the difficulty in experimental manipulation and data analysis can deter beginners. This review will introduce the basic theory, general experimental setup and data analysis of SANS and NR, followed by provision of their applications in characterising interfacial and solution self-assemblies of representative peptides and proteins. SANS and NR are remarkably effective in determining the morphological features self-assembled short peptides, especially size and shape transitions as a result of either sequence changes or in response to environmental stimuli, demonstrating the unique capability of NR and SANS in unravelling the interactive processes. These examples highlight the potential of NR and SANS in supporting the development of novel short peptides and proteins as biopharmaceutical candidates in the fight against many diseases and infections that share common features of membrane interactive processes., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
42. Meta-genome analysis of a newly enriched azo dyes detoxification halo-thermophilic bacterial consortium.
- Author
-
Tian F, Wang Y, Guo G, Ding K, Yang F, Wang C, Wang H, and Yan M
- Abstract
Treating textile wastewaters were always inhibited by its higher salt concentration and temperature. In this study, a halo-thermophilic bacterial consortium YM was enriched with ability to decolorize acid brilliant scarlet GR (ABS) at 55 °C and 10% salinity. Under optimum conditions of pH (8), temperature (55 °C), and salinity (10%), YM decolorized 97% of ABS under anaerobic conditions. Alteribacillus was identified to be the dominant genus in consortium YM. Consortium YM showed significant decolorization ability under a wide range of salinity (1%-10%), pH (7-9) and temperature (45 °C-60 °C). The degradation pathway of ABS was proposed by the combination of UV-vis spectral analysis, Fourier transform infrared (FTIR), gas chromatography mass spectrometric (GC-MS), and metagenomic analysis. Azoreductase, which was an important enzyme in decolorization process, was identified with great variation in the genome of consortium YM. Meanwhile, the metabolic intermediates after decolorization was identified with low biotoxicity by phytotoxicity tests. This study first identified that Alterbacillus play an important role in azo dye decolorization and degradation process under halo-thermophlic conditions and provided significant knowledge for azo dye decolorization and degradation process., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interestsChongyang Wang reports financial support was provided by the Key Scientific Research Project in Colleges and Universities of Henan Province of China., (Copyright © 2023 Elsevier Inc. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
43. Metabolomic insights into neurological effects of BDE-47 exposure in the sea cucumber Apostichopus japonicus.
- Author
-
Ding K, Xu Q, Zhang X, and Liu S
- Subjects
- Animals, Halogenated Diphenyl Ethers toxicity, Halogenated Diphenyl Ethers metabolism, Stichopus, Sea Cucumbers, Physiological Phenomena
- Abstract
The persistent organic pollutant 2,2',4,4'-Tetrabromodiphenyl ether (BDE-47), a prevalent congener among polybrominated diphenyl ethers (PBDEs), exhibits potent bioaccumulation and toxicity. Despite extensive research into the adverse effects of BDE-47, its neurotoxicity in sea cucumbers remains unexplored. Given the crucial role of the sea cucumber's nervous system in survival and adaptation, evaluating the impacts of BDE-47 is vital for sustainable aquaculture and consumption. In this study, we employed ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Triple-TOF-MS) to analyze metabolomic changes in neuro-related tissues of Apostichopus japonicus exposed to low (0.1 µg/L), medium (1.0 µg/L), and high (10.0 µg/L) BDE-47 concentrations. We identified significantly changed metabolites in each exposure group (87 in low, 79 in medium, and 102 in high), affecting a variety of physiological processes such as steroid hormone balance, nucleotide metabolism, energy metabolism, neurotransmitter levels, and neuroprotection. In addition, we identified concentration-dependent, common, and some other metabolic responses in the neuro-related tissues. Our findings reveal critical insights into the neurotoxic effects of BDE-47 in sea cucumbers and contribute to risk assessment related to BDE-47 exposure in the sea cucumber industry, paving the way for future neurotoxicological research in invertebrates., Competing Interests: Declaration of Competing Interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
44. Inhibitory control associated with the neural mechanism of joint attention in preschoolers: An fNIRS evidence.
- Author
-
Ding K, Wang H, Wang Q, Li H, and Li C
- Subjects
- Male, Child, Preschool, Humans, Prefrontal Cortex diagnostic imaging, Spectroscopy, Near-Infrared, Attention, Executive Function, Cognition
- Abstract
Joint attention (JA) is fundamental to the development of children's social functioning; both its response and initiation are closely related to executive function (EF), but the relationship between JA and EF has been relatively rarely studied. The present study aimed to investigate the between-condition differences in brain activation and synchronization of JA under four conditions: (1) stranger-Initiating Joint Attention (Stranger-IJA); (2) teacher-Initiating Joint Attention (Teacher-IJA); (3) stranger-Responding to Joint Attention (Stranger-RJA); and (4) teacher-Responding to Joint Attention (Teacher-RJA). It also aimed to explore the relationships between neuroimaging data and children's inhibitory control levels. To address these two goals, the present study employed 41 (aged 58.61 ± 8.64 months, 24 boys) preschool children through behavioral and functional Near-Infrared Spectroscopy (fNIRS) brain imaging assessment to measure children's EF and brain function under JA, respectively. The results revealed that: (1) a significantly higher prefrontal cortex (PFC) activation was triggered in IJA than RJA; (2) a significantly higher brain activation was triggered in JA with a stranger than with a teacher; (3) a significantly higher index of synchronization asymmetry was evoked in the left and right PFC during interaction with the teacher than with the stranger; and (4) preschoolers' brain activation and synchronization were correlated with their inhibitory control level. The findings advance our understanding of preschoolers' social cognitive development with a biological aspect, offer an opportunity to understand the potential risk of the neural disorder in preschoolers, and provide a basis and insight for preventing neural developmental disorders., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
45. Hepatic glucuronyl C5-epimerase combats obesity by stabilising GDF15.
- Author
-
He F, Jiang H, Peng C, Wang T, Xiao R, Chen M, Song N, Du Z, Wang H, Ding X, Shao Y, Fang J, Zang Y, Hua R, Li J, and Ding K
- Subjects
- Animals, Mice, Diet, High-Fat, Lipids, Liver metabolism, Racemases and Epimerases metabolism, Growth Differentiation Factor 15 metabolism, Obesity metabolism
- Abstract
Background & Aims: Disturbed hepatic metabolism frequently results in excessive lipid accumulation in the adipose tissue. However, the specific role of the liver-adipose axis in maintaining lipid homeostasis, as well as the underlying mechanism, has not yet been fully elucidated. In this study, we investigated the role of hepatic glucuronyl C5-epimerase (Glce) in the progression of obesity., Methods: We determined the association between the expression of hepatic Glce and body mass index (BMI) in obese patients. Obesity models were established in hepatic Glce-knockout and wild-type mice fed a high-fat diet (HFD) to understand the effect of Glce on obesity development. The role of Glce in the progression of disrupted hepatokine secretion was examined via secretome analysis., Results: Hepatic Glce expression was inversely correlated with BMI in obese patients. Moreover, Glce level was found to be decreased in the liver of a HFD murine model. Hepatic Glce deficiency led to impaired thermogenesis in adipose tissue and exacerbated HFD-induced obesity. Interestingly, decreased level of growth differentiation factor 15 (GDF15) was observed in the culture medium of Glce-knockout mouse hepatocytes. Treatment with recombinant GDF15 obstructed obesity progression derived from the absence of hepatic Glce, similar to the effect of Glce or its inactive mutant overexpressed both in vitro and in vivo. Furthermore, liver Glce deficiency led to diminished production and increased degradation of mature GDF15, resulting in reduced hepatic GDF15 secretion., Conclusions: Hepatic Glce deficiency facilitated obesity development, and decreased Glce expression further reduced hepatic secretion of GDF15, thereby perturbing lipid homeostasis in vivo. Therefore, the novel Glce-GDF15 axis plays an important role in maintaining energy balance and may act as a potential target for combating obesity., Impact and Implications: Evidence suggests that GDF15 plays a key role in hepatic metabolism; however, the molecular mechanism for regulating its expression and secretion is largely unknown. Our work observes that hepatic Glce, as a key Golgi-localised epimerase, may work on the maturation and post-translational regulation of GDF15. Hepatic Glce deficiency reduces the production of mature GDF15 protein and facilitates its ubiquitination, resulting in the aggravation of obesity development. This study sheds light on the new function and mechanism of the Glce-GDF15 axis in lipid metabolism and provides a potential therapeutic target against obesity., (Copyright © 2023 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
46. A novel peptidoglycan isolated from Semiaquilegia adoxoides inhibits Aβ 42 production via activating autophagy.
- Author
-
Li S, Wu F, Gao P, Jin C, Wang Y, Liao W, and Ding K
- Subjects
- Mice, Animals, Amyloid beta-Peptides, Amyloid Precursor Protein Secretases, Peptidoglycan, Aspartic Acid Endopeptidases metabolism, Molecular Structure, Autophagy, Polysaccharides, Semiaquilegia, Alzheimer Disease drug therapy
- Abstract
The accumulation of amyloid β (Aβ) containing senile plaques is one of the key histopathological hallmarks of Alzheimer's disease (AD). Increasing evidences demonstrated the important role of autophagy in Aβ clearance. Recent studies implied that extracts from Semiaquilegia adoxoides (DC.) Makino could ameliorate the memory of D-galactose induced aging mice. However, the bioactive substance and underlying mechanism remains unknown. Thus, the present study sought to explore the effects of a novel homogenous peptidoglycan on Aβ
42 secretion and the underlying mechanism. Briefly, we extracted a novel peptidoglycan named SA02C using hot water extraction and alcohol precipitation with the Mw of 13.72 kDa. SA02C contains 73.33% carbohydrate and 27.83% protein. The structure characterization revealed that its glycan part might mainly composed of galacturonic acid with minor rhamnose in backbone, and branched with glucose, galactose, arabinose, xylose and galacturonic acid. The protein or peptide moiety in SA02C was bonded to the polysaccharide via threonine. Bioactivities test showed that SA02C could reduce Aβ42 production in a dose dependent manner with no obvious cytotoxicity. Mechanism study demonstrated that SA02C could modulate APP processing by upregulating the expression of ADAM10, sAPPα and downregulating BACE1, sAPPβ. Furthermore, SA02C also could stimulate autophagy by promoting the expression of the markers of autophagy such as LC3B and ATG5, resulting in the promotion of Aβ42 phagocytosis., Competing Interests: Declaration of Competing Interest The authors indicated no conflicts of interest with regard to the content of this article., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2023
- Full Text
- View/download PDF
47. A pectic polysaccharide isolated from Achyranthes bidentata is metabolized by human gut Bacteroides spp.
- Author
-
Wen C, Li T, Wang B, Jin C, Li S, Li Y, Li M, and Ding K
- Subjects
- Humans, Pectins, Galactose, Arabinose metabolism, Polysaccharides chemistry, Glucose, Glucuronic Acid, Achyranthes chemistry, Bacteroides thetaiotaomicron metabolism
- Abstract
Achyranthes bidentata (A. bidentata) is a famous traditional Chinese medicine (TGM) for treatment osteoporosis. Polysaccharides, a major factor for shaping the gut microbiota, are the primary ingredients of A. bidentata. However, bioactivity of A. bidentata polysaccharide on human gut microbiota (HGM) remains unknown. Here, a homogeneous pectic polysaccharide A23-1 with average molecular weight of 93.085 kDa was extracted and purified from A. bidentata. And A23-1 was compsed of rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose in a molar ratio of 7.26: 0.76: 5.12: 2.54: 23.51: 60.81. GC-MS, partial acid hydrolysis and NMR results indicated the backbone of A23-1 was composed of 1, 2, 4-Rhap and 1, 4-GlapA, while the branches were composed of galactose, arabinose, glucose and glucuronic acid. Further, A23-1 was found to be degraded into monosaccharides and fragments. Taking Bacteroides thetaiotaomicron (BT) as a model, we suggested three polysaccharide utilization loci (PULs) might be involved in the A23-1 degradation. Degraded products generated by BO might not support the growth of probiotics. Besides, acetate and propionate as the main end products were generated by Bacteroides spp. and probiotics utilizing A23-1. These findings suggested A23-1 was possible one of food sources of human gut Bacteroides spp., Competing Interests: Declaration of competing interest The authors declare no competing interests., (Copyright © 2023. Published by Elsevier B.V.)
- Published
- 2023
- Full Text
- View/download PDF
48. Small diameter expanded polytetrafluoroethylene vascular graft with differentiated inner and outer biomacromolecules for collaborative endothelialization, anti-thrombogenicity and anti-inflammation.
- Author
-
Ding K, Yu X, Wang D, Wang X, and Li Q
- Subjects
- Humans, Polytetrafluoroethylene pharmacology, Polytetrafluoroethylene chemistry, Prospective Studies, Heparin pharmacology, Human Umbilical Vein Endothelial Cells, Sirolimus, Blood Vessel Prosthesis, Thrombosis drug therapy
- Abstract
Without differentiated inner and outer biological function, expanded polytetrafluoroethylene (ePTFE) small-diameter (<6 mm) artificial blood vessels would fail in vivo due to foreign body rejection, thrombosis, and hyperplasia. In order to synergistically promote endothelialization, anti-thrombogenicity, and anti-inflammatory function, we modified the inner and outer surface of ePTFE, respectively, by grafting functional biomolecules, such as heparin and epigallocatechin gallate (EGCG), into the inner surface and polyethyleneimine and rapamycin into the outer surface via layer-by-layer self-assembly. Fourier-transform infrared spectroscopy showed the successful incorporation of EGCG, heparin, and rapamycin. The collaborative release profile of heparin and rapamycin lasted for 42 days, respectively. The inner surface promoted human umbilical vein endothelial cells (HUVECs) adhesion and growth and that the outer surface inhibited smooth muscle cells growth and proliferation. The modified ePTFE effectively regulated the differentiation behavior of RAW264.7, inhibited the expression of proinflammatory mediator TNF-α, and up-regulated the expression of anti-inflammatory genes Arg1 and Tgfb-1. The ex vivo circulation results indicated that the occlusions and total thrombus weight of modified ePTFE was much lower than that of the thrombus formed on the ePTFE, presenting good anti-thrombogenic properties. Hence, the straightforward yet efficient synergistic surface functionalization approach presented a potential resolution for the prospective clinical application of small-diameter ePTFE blood vessel grafts., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Qian Li reports was provided by International Science and Technology Cooperation Program of China., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
49. A novel branched galacturonan from Gardenia jasminoides alleviates liver fibrosis linked to TLR4/NF-κB signaling.
- Author
-
Ma X, Zhou W, Nie Y, Jing X, Li S, Jin C, Zhu A, Su J, Liao W, and Ding K
- Abstract
Gardenia jasminoides (GJ) is a classic edible medicine in China of which the fruit has been proved to alleviate liver damage. We hypothesized whether polysaccharide in the fruit could have comparable bioactivity. To address this, a novel polysaccharide GJE0.2-2, is purified from the fruit of Gardenia jasminoides. Indeed, GJE0.2-2 may attenuate CCl
4 -induced liver fibrosis in mice and impede the expression of critical fibrogenesis associated molecules such as α-SMA, FN1, and Collagen I induced by TGF-β in human hepatic stellate LX-2 cells. Mechanism studies suggest that this bioactivity may be implicated in TLR4/NF-κB signaling pathway via directly binding to TLR4. The structure characterization shows that the backbone of this polysaccharide is mainly composed of galacturonic acid with minor rhamnose, branched with galactose and arabinose, galacturonic acid, and esterified hexenuronic acid (HexpA). These findings provide evidence for a novel pectin-linked polysaccharide-based new drug candidate development for liver fibrosis therapy., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2023
- Full Text
- View/download PDF
50. Overcoming tumor microenvironment obstacles: Current approaches for boosting nanodrug delivery.
- Author
-
Wang X, Zhang H, Chen X, Wu C, Ding K, Sun G, Luo Y, and Xiang D
- Subjects
- Humans, Tumor Microenvironment, Nanomedicine methods, Drug Delivery Systems methods, Neoplasms drug therapy, Antineoplastic Agents pharmacology, Antineoplastic Agents therapeutic use, Nanoparticles therapeutic use, Nanoparticles chemistry
- Abstract
In order to achieve targeted delivery of anticancer drugs, efficacy improvement, and side effect reduction, various types of nanoparticles are employed. However, their therapeutic effects are not ideal. This phenomenon is caused by tumor microenvironment abnormalities such as abnormal blood vessels, elevated interstitial fluid pressure, and dense extracellular matrix that affect nanoparticle penetration into the tumor's interstitium. Furthermore, nanoparticle properties including size, charge, and shape affect nanoparticle transport into tumors. This review comprehensively goes over the factors hindering nanoparticle penetration into tumors and describes methods for improving nanoparticle distribution by remodeling the tumor microenvironment and optimizing nanoparticle physicochemical properties. Finally, a critical analysis of future development of nanodrug delivery in oncology is further discussed. STATEMENT OF SIGNIFICANCE: This article reviews the factors that hinder the distribution of nanoparticles in tumors, and describes existing methods and approaches for improving the tumor accumulation from the aspects of remodeling the tumor microenvironment and optimizing the properties of nanoparticles. The description of the existing methods and approaches is followed by highlighting their advantages and disadvantages and put forward possible directions for the future researches. At last, the challenges of improving tumor accumulation in nanomedicines design were also discussed. This review will be of great interest to the broad readers who are committed to delivering nanomedicine for cancer treatment., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.