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2 results on '"Els Willems"'

1. Differential effects of inulin or its fermentation metabolites on gut barrier and immune function of porcine intestinal epithelial cells

Author

Julie Uerlings, Martine Schroyen, Els Willems, Sofie Tanghe, Geert Bruggeman, Jérôme Bindelle, and Nadia Everaert

Subjects
Prebiotic, In vitro fermentation, Porcine intestinal epithelial cells, Short-chain fatty acids, Gut barrier, Inflammatory response, Nutrition. Foods and food supply, TX341-641
Abstract

Prebiotics can modulate gut fermentation and improve intestinal barrier function in mammals. First, inulin fermentation profile was tested in a three-step in vitro model of the piglet’s gastro-intestinal tract combining a hydrolysis – dialysis step to a batch fermentation. Then, the differential effects of digested inulin (after the hydrolysis – dialysis steps) or fermented inulin (after the fermentation step) on the expression of gut barrier and immune-related genes of IPEC-J2 cells were investigated by high-throughput qPCR. Inulin was associated with elevated short-chain fatty acids and butyrate levels. Upregulated expressions of tight and adherens junction genes were observed in IPEC-J2 cells supplemented with inulin fermentation supernatant compared to control IPEC-J2 cells and digested inulin. Therefore, metabolites arising from the fermentation process, including butyrate, could be responsible for the reinforcement of the barrier function.

Published
2020
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2. Differential effects of inulin or its fermentation metabolites on gut barrier and immune function of porcine intestinal epithelial cells

Author

Nadia Everaert, Geert Bruggeman, Jérôme Bindelle, Julie Uerlings, Sofie Tanghe, Els Willems, and Martine Schroyen

Subjects
0301 basic medicine, In vitro fermentation, Inulin, Porcine intestinal epithelial cells, Medicine (miscellaneous), Prebiotic, Butyrate, ARABINOXYLAN, ACTIVATION, Adherens junction, 03 medical and health sciences, chemistry.chemical_compound, Hydrolysis, Short-chain fatty acids, BUTYRATE, 0404 agricultural biotechnology, Immune system, Downregulation and upregulation, Gut barrier, TX341-641, PERMEABILITY, Barrier function, Science & Technology, 030109 nutrition & dietetics, Nutrition and Dietetics, Nutrition & Dietetics, Nutrition. Foods and food supply, food and beverages, Inflammatory response, IN-VITRO FERMENTATION, 04 agricultural and veterinary sciences, 040401 food science, PRODUCTS, OLIGOSACCHARIDES, FRUCTANS, MODEL, chemistry, Biochemistry, ESCHERICHIA-COLI, Food Science & Technology, Fermentation, Life Sciences & Biomedicine, Food Science
Abstract

Prebiotics can modulate gut fermentation and improve intestinal barrier function in mammals. First, inulin fermentation profile was tested in a three-step in vitro model of the piglet’s gastro-intestinal tract combining a hydrolysis – dialysis step to a batch fermentation. Then, the differential effects of digested inulin (after the hydrolysis – dialysis steps) or fermented inulin (after the fermentation step) on the expression of gut barrier and immune-related genes of IPEC-J2 cells were investigated by high-throughput qPCR. Inulin was associated with elevated short-chain fatty acids and butyrate levels. Upregulated expressions of tight and adherens junction genes were observed in IPEC-J2 cells supplemented with inulin fermentation supernatant compared to control IPEC-J2 cells and digested inulin. Therefore, metabolites arising from the fermentation process, including butyrate, could be responsible for the reinforcement of the barrier function.

Published
2020

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