22 results on '"Epel, Boris"'
Search Results
2. Directional TV algorithm for fast EPR imaging.
- Author
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Fang C, Xi Y, Epel B, Halpern H, and Qiao Z
- Subjects
- Electron Spin Resonance Spectroscopy methods, Phantoms, Imaging, Oxygen, Image Processing, Computer-Assisted methods, Algorithms, Imaging, Three-Dimensional methods
- Abstract
Precise radiation guided by oxygen images has demonstrated superiority over the traditional radiation methods. Electron paramagnetic resonance (EPR) imaging has proven to be the most advanced oxygen imaging modality. However, the main drawback of EPR imaging is the long scan time. For each projection, we usually need to collect the projection many times and then average them to achieve high signal-to-noise ratio (SNR). One approach to fast scan is to reduce the repeating time for each projection. While the projections would be noisy and thus the traditional commonly-use filtered backprojection (FBP) algorithm would not be capable of accurately reconstructing images. Optimization-based iterative algorithms may accurately reconstruct images from noisy projections for they may incorporate prior information into optimization models. Based on the total variation (TV) algorithms for EPR imaging, in this work, we propose a directional TV (DTV) algorithm to further improve the reconstruction accuracy. We construct the DTV constrained, data divergence minimization (DTVcDM) model, derive its Chambolle-Pock (CP) solving algorithm, validate the correctness of the whole algorithm, and perform evaluations via simulated and real data. The experimental results show that the DTV algorithm outperforms the existing TV and FBP algorithms in fast EPR imaging. Compared to the standard FBP algorithm, the proposed algorithm may achieve 10 times of acceleration., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.)
- Published
- 2024
- Full Text
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3. Accurate reconstruction of 4D spectral-spatial images from sparse-view data in continuous-wave EPRI.
- Author
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Zhang Z, Epel B, Chen B, Xia D, Sidky EY, Halpern H, and Pan X
- Abstract
In continuous-wave electron paramagnetic resonance imaging (CW EPRI), data are collected generally at densely sampled views sufficient for achieving accurate reconstruction of a four dimensional spectral-spatial (4DSS) image by use of the conventional filtered-backprojection (FBP) algorithm. It is desirable to minimize the scan time by collection of data only at sparsely sampled views, referred to as sparse-view data. Interest thus remains in investigation of algorithms for accurate reconstruction of 4DSS images from sparse-view data collected for potentially enabling fast data acquisition in CW EPRI. In this study, we investigate and demonstrate optimization-based algorithms for accurate reconstruction of 4DSS images from sparse-view data. Numerical studies using simulated and real sparse-view data acquired in CW EPRI are conducted that reveal, in terms of image visualization and physical-parameter estimation, the potential of the algorithms developed for yielding accurate 4DSS images from sparse-view data in CW EPRI. The algorithms developed may be exploited for enabling sparse-view scans with minimized scan time in CW EPRI for yielding 4DSS images of quality comparable to, or better than, that of the FBP reconstruction from data collected at densely sampled views., Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Xiaochuan Pan reports financial support was provided by National Institutes of Health. Boris Epel, Howard Halpern reports a relationship with O2M Technologies LLC that includes: board membership and equity or stocks. Howard Halpern has patent #8664955, 10568537, and 9392957. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024. Published by Elsevier Inc.)
- Published
- 2024
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4. 4D-image reconstruction directly from limited-angular-range data in continuous-wave electron paramagnetic resonance imaging.
- Author
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Zhang Z, Epel B, Chen B, Xia D, Sidky EY, Qiao Z, Halpern H, and Pan X
- Abstract
Objective: We investigate and develop optimization-based algorithms for accurate reconstruction of four-dimensional (4D)-spectral-spatial (SS) images directly from data collected over limited angular ranges (LARs) in continuous-wave (CW) electron paramagnetic resonance imaging (EPRI)., Methods: Basing on a discrete-to-discrete data model devised in CW EPRI employing the Zeeman-modulation (ZM) scheme for data acquisition, we first formulate the image reconstruction problem as a convex, constrained optimization program that includes a data fidelity term and also constraints on the individual directional total variations (DTVs) of the 4D-SS image. Subsequently, we develop a primal-dual-based DTV algorithm, simply referred to as the DTV algorithm, to solve the constrained optimization program for achieving image reconstruction from data collected in LAR scans in CW-ZM EPRI., Results: We evaluate the DTV algorithm in simulated- and real-data studies for a variety of LAR scans of interest in CW-ZM EPRI, and visual and quantitative results of the studies reveal that 4D-SS images can be reconstructed directly from LAR data, which are visually and quantitatively comparable to those obtained from data acquired in the standard, full-angular-range (FAR) scan in CW-ZM EPRI., Conclusion: An optimization-based DTV algorithm is developed for accurately reconstructing 4D-SS images directly from LAR data in CW-ZM EPRI. Future work includes the development and application of the optimization-based DTV algorithm for reconstructions of 4D-SS images from FAR and LAR data acquired in CW EPRI employing schemes other than the ZM scheme., Significance: The DTV algorithm developed may be exploited potentially for enabling and optimizing CW EPRI with minimized imaging time and artifacts by acquiring data in LAR scans., Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: [Authors HH and BE declare US patents 8,664,955, 10,568,537, and 9,392,957 on aspects of the pO2 imaging technology and membership in a start up company O2M Technologies. Other authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.], (Copyright © 2023 Elsevier Inc. All rights reserved.)
- Published
- 2023
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5. An iterative reconstruction algorithm without system matrix for EPR imaging.
- Author
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Qiao Z, Lu Y, Liu P, Epel B, and Halpern H
- Subjects
- Electron Spin Resonance Spectroscopy methods, Phantoms, Imaging, Image Processing, Computer-Assisted methods, Algorithms, Oxygen
- Abstract
Electron paramagnetic resonance (EPR) imaging is an advanced oxygen imaging modality for oxygen-image guided radiation. The iterative reconstruction algorithm is the research hot-point in image reconstruction for EPR imaging (EPRI) for this type of algorithm may incorporate image-prior information to construct advanced optimization model to achieve accurate reconstruction from sparse-view projections and/or noisy projections. However, the system matrix in the iterative algorithm needs complicated calculation and needs huge memory-space if it is stored in memory. In this work, we propose an iterative reconstruction algorithm without system matrix for EPRI to simplify the whole iterative reconstruction process. The function of the system matrix is to calculate the projections, whereas the function of the transpose of the system matrix is to perform backprojection. The existing projection and backprojection methods are all based on the configuration that the imaged-object remains stationary and the scanning device rotates. Here, we implement the projection and backprojection operations by fixing the scanning device and rotating the object. Thus, the core algorithm is only the commonly-used image-rotation algorithm, while the calculation and store of the system matrix are avoided. Based on the idea of image rotation, we design a specific iterative reconstruction algorithm for EPRI, total variation constrained data divergence minimization (TVcDM) algorithm without system matrix, and named it as image-rotation based TVcDM (R-TVcDM). Through a series of comparisons with the original TVcDM via real projection data, we find that the proposed algorithm may achieve similar reconstruction accuracy with the original one. But it avoids the complicated calculation and store of the system matrix. The insights gained in this work may be also applied to other imaging modalities, for example computed tomography and positron emission tomography., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Inc. All rights reserved.)
- Published
- 2022
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6. A balanced total-variation-Chambolle-Pock algorithm for EPR imaging.
- Author
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Qiao Z, Redler G, Epel B, and Halpern H
- Subjects
- Electron Spin Resonance Spectroscopy, Imaging, Three-Dimensional, Phantoms, Imaging, Tomography, X-Ray Computed, Algorithms, Image Processing, Computer-Assisted
- Abstract
Total variation (TV) minimization algorithm is an effective algorithm capable of accurately reconstructing images from sparse projection data in a variety of imaging modalities including computed tomography (CT) and electron paramagnetic resonance imaging (EPRI). The data divergence constrained, TV minimization (DDcTV) model and its Chambolle-Pock (CP) solving algorithm have been proposed for CT. However, when the DDcTV-CP algorithm is applied to 3D EPRI, it suffers from slow convergence rate or divergence. We hypothesize that this is due to the magnitude imbalance between the data fidelity term and the TV regularization term. In this work, we propose a balanced TV (bTV) model incorporating a balance parameter and demonstrate its capability to avoid convergence issues for the 3D EPRI application. Simulation and real experiments show that the DDcTV-CP algorithm cannot guarantee convergence but the bTV-CP algorithm may guarantee convergence and achieve fast convergence by use of an appropriate balance parameter. Experiments also show that underweighting the balance parameter leads to slow convergence, whereas overweighting the balance parameter leads to divergence. The iteration-behavior change-law with the variation of the balance parameter is explained by use of the data tolerance ellipse and gradient descent principle. The findings and insights gained in this work may be applied to other imaging modalities and other constrained optimization problems., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Published
- 2021
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7. Modular imaging system: Rapid scan EPR at 800 MHz.
- Author
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Tseytlin O, Guggilapu P, Bobko AA, AlAhmad H, Xu X, Epel B, O'Connell R, Hoblitzell EH, Eubank TD, Khramtsov VV, Driesschaert B, Kazkaz E, and Tseytlin M
- Subjects
- Algorithms, Animals, Equipment Design, Image Processing, Computer-Assisted, Mice, Phantoms, Imaging, Signal Processing, Computer-Assisted, Electron Spin Resonance Spectroscopy instrumentation, Mammary Neoplasms, Experimental diagnostic imaging
- Abstract
An electron paramagnetic resonance (EPR) imaging system has been custom built for use in pre-clinical and, potentially, clinical studies. Commercial standalone modules have been used in the design that are MATLAB-controlled. The imaging system combines digital and analog technologies. It was designed to achieve maximum flexibility and versatility and to perform standard and novel user-defined experiments. This design goal is achieved by frequency mixing of an arbitrary waveform generator (AWG) output at the intermediate frequency (IF) with a constant source frequency (SF). Low noise SF at 250, 750, and 1000 MHz are available in the system. A wide range of frequencies from near-baseband to L-band can be generated as a result. Two-stage downconversion at the signal detection side is implemented that enables multi-frequency EPR capability. In the first stage, the signal frequency is converted to IF. A novel AWG-enabled digital auto-frequency control method that operates at IF is described that is used for automatic resonator tuning. Quadrature baseband EPR signal is generated in the second downconversion step. The semi-digital approach of mixing low-noise frequency sources with an AWG permits generation of arbitrary excitation patterns that include but are not limited to frequency sweeps for resonator tuning and matching, continuous-wave, and pulse sequences. Presented in this paper is the demonstration of rapid scan (RS) EPR imaging implemented at 800 MHz. Generation of stable magnetic scan waveforms is critical for the RS method. A digital automatic scan control (DASC) system was developed for sinusoidal magnetic field scans. DASC permits tight control of both amplitude and phase of the scans. A surface loop resonator was developed using 3D printing technology. RS EPR imaging system was validated using sample phantoms. In vivo imaging of a breast cancer mouse model is demonstrated., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2019
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8. Optimization-based image reconstruction from sparsely sampled data in electron paramagnetic resonance imaging.
- Author
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Qiao Z, Zhang Z, Pan X, Epel B, Redler G, Xia D, and Halpern H
- Subjects
- Algorithms, Animals, Artifacts, Computer Simulation, Imaging, Three-Dimensional, Phantoms, Imaging, Reproducibility of Results, Electron Spin Resonance Spectroscopy methods, Image Processing, Computer-Assisted methods
- Abstract
Electron paramagnetic resonance imaging (EPRI) can yield information about the 3-dimensional (3D) spatial distribution of the unpaired-electron-spin density from which the spatial distribution of oxygen concentration within tumor tissue, referred to as the oxygen image or electron paramagnetic resonance (EPR) image in this work, can be derived. Existing algorithms for reconstruction of EPR images often require data collected at a large number of densely sampled projection views, resulting in a prolonged data-acquisition time and consequently numerous practical challenges especially to in vivo animal EPRI. Therefore, a strong interest exists in shortening data-acquisition time through reducing the number of data samples collected in EPRI, and one approach is to acquire data at a reduced number of sparsely distributed projection views from which existing algorithms may reconstruct images with prominent artifacts. In this work, we investigate and develop an optimization-based technique for image reconstruction from data collected at sparsely sampled projection views for reducing scanning time in EPRI. Specifically, we design a convex optimization program in which the EPR image of interest is formulated as a solution and then tailor the Chambolle-Pock (CP) primal-dual algorithm to reconstruct the image by solving the convex optimization program. Using computer-simulated EPRI data from numerical phantoms and real EPRI data collected from physical phantoms, we perform studies on the verification and characterization of the optimization-based technique for EPR image reconstruction. Results of the studies suggest that the technique may yield accurate EPR images from data collected at sparsely distributed projection views, thus potentially enabling fast EPRI with reduced acquisition time., (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Published
- 2018
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9. Integration of a versatile bridge concept in a 34 GHz pulsed/CW EPR spectrometer.
- Author
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Band A, Donohue MP, Epel B, Madhu S, and Szalai VA
- Abstract
We present a 34 GHz continuous wave (CW)/pulsed electron paramagnetic resonance (EPR) spectrometer capable of pulse-shaping that is based on a versatile microwave bridge design. The bridge radio frequency (RF)-in/RF-out design (500 MHz to 1 GHz input/output passband, 500 MHz instantaneous input/output bandwidth) creates a flexible platform with which to compare a variety of excitation and detection methods utilizing commercially available equipment external to the bridge. We use three sources of RF input to implement typical functions associated with CW and pulse EPR spectroscopic measurements. The bridge output is processed via high speed digitizer and an in-phase/quadrature (I/Q) demodulator for pulsed work or sent to a wideband, high dynamic range log detector for CW. Combining this bridge with additional commercial hardware and new acquisition and control electronics, we have designed and constructed an adaptable EPR spectrometer that builds upon previous work in the literature and is functionally comparable to other available systems., (Published by Elsevier Inc.)
- Published
- 2018
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10. In vivo preclinical cancer and tissue engineering applications of absolute oxygen imaging using pulse EPR.
- Author
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Epel B, Kotecha M, and Halpern HJ
- Subjects
- Animals, Humans, Oxygen chemistry, Reproducibility of Results, Electron Spin Resonance Spectroscopy methods, Molecular Imaging methods, Neoplasms diagnostic imaging, Tissue Engineering methods
- Abstract
The value of any measurement and a fortiori any measurement technology is defined by the reproducibility and the accuracy of the measurements. This implies a relative freedom of the measurement from factors confounding its accuracy. In the past, one of the reasons for the loss of focus on the importance of imaging oxygen in vivo was the difficulty in obtaining reproducible oxygen or pO
2 images free from confounding variation. This review will briefly consider principles of electron paramagnetic oxygen imaging and describe how it achieves absolute oxygen measurements. We will provide a summary review of the progress in biomedical EPR imaging, predominantly in cancer biology research, discuss EPR oxygen imaging for cancer treatment and tissue graft assessment for regenerative medicine applications., (Copyright © 2017. Published by Elsevier Inc.)- Published
- 2017
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11. Rapid-scan EPR imaging.
- Author
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Eaton SS, Shi Y, Woodcock L, Buchanan LA, McPeak J, Quine RW, Rinard GA, Epel B, Halpern HJ, and Eaton GR
- Subjects
- Algorithms, Animals, Diagnostic Imaging, Humans, Microwaves, Electron Spin Resonance Spectroscopy methods, Molecular Imaging methods
- Abstract
In rapid-scan EPR the magnetic field or frequency is repeatedly scanned through the spectrum at rates that are much faster than in conventional continuous wave EPR. The signal is directly-detected with a mixer at the source frequency. Rapid-scan EPR is particularly advantageous when the scan rate through resonance is fast relative to electron spin relaxation rates. In such scans, there may be oscillations on the trailing edge of the spectrum. These oscillations can be removed by mathematical deconvolution to recover the slow-scan absorption spectrum. In cases of inhomogeneous broadening, the oscillations may interfere destructively to the extent that they are not visible. The deconvolution can be used even when it is not required, so spectra can be obtained in which some portions of the spectrum are in the rapid-scan regime and some are not. The technology developed for rapid-scan EPR can be applied generally so long as spectra are obtained in the linear response region. The detection of the full spectrum in each scan, the ability to use higher microwave power without saturation, and the noise filtering inherent in coherent averaging results in substantial improvement in signal-to-noise relative to conventional continuous wave spectroscopy, which is particularly advantageous for low-frequency EPR imaging. This overview describes the principles of rapid-scan EPR and the hardware used to generate the spectra. Examples are provided of its application to imaging of nitroxide radicals, diradicals, and spin-trapped radicals at a Larmor frequency of ca. 250MHz., (Copyright © 2017 Elsevier Inc. All rights reserved.)
- Published
- 2017
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12. Imaging thiol redox status in murine tumors in vivo with rapid-scan electron paramagnetic resonance.
- Author
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Epel B, Sundramoorthy SV, Krzykawska-Serda M, Maggio MC, Tseytlin M, Eaton GR, Eaton SS, Rosen GM, Kao JPY, and Halpern HJ
- Subjects
- Animals, Buthionine Sulfoximine chemistry, Disulfides chemistry, Female, Glutathione metabolism, Imaging, Three-Dimensional, Kinetics, Mice, Mice, Inbred C3H, Neoplasms, Experimental metabolism, Nitrogen Oxides chemistry, Oxidation-Reduction, Signal-To-Noise Ratio, Spin Labels chemical synthesis, Brain Neoplasms diagnostic imaging, Diagnostic Imaging methods, Electron Spin Resonance Spectroscopy methods, Fibrosarcoma diagnostic imaging, Neoplasms, Experimental diagnostic imaging
- Abstract
Thiol redox status is an important physiologic parameter that affects the success or failure of cancer treatment. Rapid scan electron paramagnetic resonance (RS EPR) is a novel technique that has shown higher signal-to-noise ratio than conventional continuous-wave EPR in in vitro studies. Here we used RS EPR to acquire rapid three-dimensional images of the thiol redox status of tumors in living mice. This work presents, for the first time, in vivo RS EPR images of the kinetics of the reaction of
2 H,15 N-substituted disulfide-linked dinitroxide (PxSSPx) spin probe with intracellular glutathione. The cleavage rate is proportional to the intracellular glutathione concentration. Feasibility was demonstrated in a FSa fibrosarcoma tumor model in C3H mice. Similar to other in vivo and cell model studies, decreasing intracellular glutathione concentration by treating mice with l-buthionine sulfoximine (BSO) markedly altered the kinetic images., (Copyright © 2016 Elsevier Inc. All rights reserved.)- Published
- 2017
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13. Decoupling of excitation and receive coils in pulsed magnetic resonance using sinusoidal magnetic field modulation.
- Author
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Tseytlin M, Epel B, Sundramoorthy S, Tipikin D, and Halpern HJ
- Subjects
- Magnetic Fields, Magnetic Resonance Spectroscopy
- Abstract
In pulsed magnetic resonance, the excitation power is many orders of magnitude larger than that induced by the spin system in the receiving coil or resonator. The receiver must be protected during and immediately after the excitation pulse to allow for the energy stored in the resonator to dissipate to a safe level. The time during which the signal is not detected, the instrumental dead-time, can be shortened by using magnetically decoupled excitation and receive coils. Such coils are oriented, with respect to each other, in a way that minimizes the total magnetic flux produced by one coil in the other. We suggest that magnetically decoupled coils can be isolated to a larger degree by tuning them to separate frequencies. Spins are excited at one frequency, and the echo signal is detected at another. Sinusoidal magnetic field modulation that rapidly changes the Larmor frequency of the spins between the excitation and detection events is used to ensure the resonance conditions for both coils. In this study, the relaxation times of trityl-CD3 were measured in a field-modulated pulsed EPR experiment and compared to results obtained using a standard spin echo method. The excitation and receive coils were tuned to 245 and 256.7MHz, respectively. Using an available rapid-scan, cross-loop EPR resonator, we demonstrated an isolation improvement of approximately 20-30dB due to frequency decoupling. Theoretical analysis, numerical simulations, and proof-of-concept experiments demonstrated that substantial excitation-detection decoupling can be achieved. A pulsed L-band system, including a small volume bi-modal resonator equipped with modulation coils, was constructed to demonstrate fivefold dead-time reduction in comparison with the standard EPR experiment. This was achieved by detuning of the excitation and receive coils by 26MHz and using sinusoidal modulation at 480kHz., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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14. Fast dynamic electron paramagnetic resonance (EPR) oxygen imaging using low-rank tensors.
- Author
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Christodoulou AG, Redler G, Clifford B, Liang ZP, Halpern HJ, and Epel B
- Subjects
- Algorithms, Animals, Hypoxia, Image Processing, Computer-Assisted, Mice, Electron Spin Resonance Spectroscopy, Neoplasms diagnostic imaging, Oxygen
- Abstract
Hypoxic tumors are resistant to radiotherapy, motivating the development of tools to image local oxygen concentrations. It is generally believed that stable or chronic hypoxia is the source of resistance, but more recent work suggests a role for transient hypoxia. Conventional EPR imaging (EPRI) is capable of imaging tissue pO2in vivo, with high pO2 resolution and 1mm spatial resolution but low imaging speed (10min temporal resolution for T1-based pO2 mapping), which makes it difficult to investigate the oxygen changes, e.g., transient hypoxia. Here we describe a new imaging method which accelerates dynamic EPR oxygen imaging, allowing 3D imaging at 2 frames per minute, fast enough to image transient hypoxia at the "speed limit" of observed pO2 change. The method centers on a low-rank tensor model that decouples the tradeoff between imaging speed, spatial coverage/resolution, and number of inversion times (pO2 accuracy). We present a specialized sparse sampling strategy and image reconstruction algorithm for use with this model. The quality and utility of the method is demonstrated in simulations and in vivo experiments in tumor bearing mice., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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15. 3D pulse EPR imaging from sparse-view projections via constrained, total variation minimization.
- Author
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Qiao Z, Redler G, Epel B, Qian Y, and Halpern H
- Subjects
- Algorithms, Animals, Image Enhancement methods, Magnetic Resonance Imaging methods, Mice, Neoplasms, Experimental pathology, Reproducibility of Results, Sensitivity and Specificity, Electron Spin Resonance Spectroscopy methods, Imaging, Three-Dimensional methods, Molecular Imaging methods, Neoplasms, Experimental metabolism, Oximetry methods, Oxygen metabolism
- Abstract
Tumors and tumor portions with low oxygen concentrations (pO2) have been shown to be resistant to radiation therapy. As such, radiation therapy efficacy may be enhanced if delivered radiation dose is tailored based on the spatial distribution of pO2 within the tumor. A technique for accurate imaging of tumor oxygenation is critically important to guide radiation treatment that accounts for the effects of local pO2. Electron paramagnetic resonance imaging (EPRI) has been considered one of the leading methods for quantitatively imaging pO2 within tumors in vivo. However, current EPRI techniques require relatively long imaging times. Reducing the number of projection scan considerably reduce the imaging time. Conventional image reconstruction algorithms, such as filtered back projection (FBP), may produce severe artifacts in images reconstructed from sparse-view projections. This can lower the utility of these reconstructed images. In this work, an optimization based image reconstruction algorithm using constrained, total variation (TV) minimization, subject to data consistency, is developed and evaluated. The algorithm was evaluated using simulated phantom, physical phantom and pre-clinical EPRI data. The TV algorithm is compared with FBP using subjective and objective metrics. The results demonstrate the merits of the proposed reconstruction algorithm., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
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16. Comparison of pulse sequences for R1-based electron paramagnetic resonance oxygen imaging.
- Author
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Epel B and Halpern HJ
- Subjects
- Animals, Diagnostic Imaging instrumentation, Electron Spin Resonance Spectroscopy instrumentation, Image Processing, Computer-Assisted, Oxidation-Reduction, Oximetry, Phantoms, Imaging, Diagnostic Imaging methods, Electron Spin Resonance Spectroscopy methods, Oxygen chemistry
- Abstract
Electron paramagnetic resonance (EPR) spin-lattice relaxation (SLR) oxygen imaging has proven to be an indispensable tool for assessing oxygen partial pressure in live animals. EPR oxygen images show remarkable oxygen accuracy when combined with high precision and spatial resolution. Developing more effective means for obtaining SLR rates is of great practical, biological and medical importance. In this work we compared different pulse EPR imaging protocols and pulse sequences to establish advantages and areas of applicability for each method. Tests were performed using phantoms containing spin probes with oxygen concentrations relevant to in vivo oxymetry. We have found that for small animal size objects the inversion recovery sequence combined with the filtered backprojection reconstruction method delivers the best accuracy and precision. For large animals, in which large radio frequency energy deposition might be critical, free induction decay and three pulse stimulated echo sequences might find better practical usage., (Copyright © 2015 Elsevier Inc. All rights reserved.)
- Published
- 2015
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17. Comparison of parabolic filtration methods for 3D filtered back projection in pulsed EPR imaging.
- Author
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Qiao Z, Redler G, Epel B, and Halpern HJ
- Subjects
- Oxygen chemistry, Reproducibility of Results, Sensitivity and Specificity, Electron Spin Resonance Spectroscopy methods, Imaging, Three-Dimensional methods, Molecular Imaging methods, Oximetry methods, Oxygen analysis, Signal Processing, Computer-Assisted
- Abstract
Pulse electron paramagnetic resonance imaging (Pulse EPRI) is a robust method for noninvasively measuring local oxygen concentrations in vivo. For 3D tomographic EPRI, the most commonly used reconstruction algorithm is filtered back projection (FBP), in which the parabolic filtration process strongly influences image quality. In this work, we designed and compared 7 parabolic filtration methods to reconstruct both simulated and real phantoms. To evaluate these methods, we designed 3 error criteria and 1 spatial resolution criterion. It was determined that the 2 point derivative filtration method and the two-ramp-filter method have unavoidable negative effects resulting in diminished spatial resolution and increased artifacts respectively. For the noiseless phantom the rectangular-window parabolic filtration method and sinc-window parabolic filtration method were found to be optimal, providing high spatial resolution and small errors. In the presence of noise, the 3 point derivative method and Hamming-window parabolic filtration method resulted in the best compromise between low image noise and high spatial resolution. The 3 point derivative method is faster than Hamming-window parabolic filtration method, so we conclude that the 3 point derivative method is optimal for 3D FBP., (Copyright © 2014. Published by Elsevier Inc.)
- Published
- 2014
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18. Locations of radical species in black pepper seeds investigated by CW EPR and 9GHz EPR imaging.
- Author
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Nakagawa K and Epel B
- Subjects
- Indoles analysis, Organometallic Compounds analysis, Phantoms, Imaging, Spin Labels, Electron Spin Resonance Spectroscopy instrumentation, Free Radicals analysis, Piper nigrum chemistry, Seeds chemistry
- Abstract
In this study, noninvasive 9GHz electron paramagnetic resonance (EPR)-imaging and continuous wave (CW) EPR were used to investigate the locations of paramagnetic species in black pepper seeds without further irradiation. First, lithium phthalocyanine (LiPC) phantom was used to examine 9GHz EPR imaging capabilities. The 9GHz EPR-imager easily resolved the LiPC samples at a distance of ∼2mm. Then, commercially available black pepper seeds were measured. We observed signatures from three different radical species, which were assigned to stable organic radicals, Fe(3+), and Mn(2+) complexes. In addition, no EPR spectral change in the seed was observed after it was submerged in distilled H2O for 1h. The EPR and spectral-spatial EPR imaging results suggested that the three paramagnetic species were mostly located at the seed surface. Fewer radicals were found inside the seed. We demonstrated that the CW EPR and 9GHz EPR imaging were useful for the determination of the spatial distribution of paramagnetic species in various seeds., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2014
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19. Orthogonal resonators for pulse in vivo electron paramagnetic imaging at 250 MHz.
- Author
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Sundramoorthy SV, Epel B, and Halpern HJ
- Subjects
- Animals, Electromagnetic Fields, Electronics, Female, Hindlimb anatomy & histology, Hindlimb pathology, Image Processing, Computer-Assisted, Mice, Mice, Inbred C3H, Neoplasms, Experimental pathology, Phantoms, Imaging, Diagnostic Imaging methods, Electron Spin Resonance Spectroscopy methods
- Abstract
A 250 MHz bimodal resonator with a 19 mm internal diameter for in vivo pulse electron paramagnetic resonance (EPR) imaging is presented. Two separate coaxial cylindrical resonators inserted one into another were used for excitation and detection. The Alderman-Grant excitation resonator (AGR) showed the highest efficiency among all the excitation resonators tested. The magnetic field of AGR is confined to the volume of the detection resonator, which results in highly efficient use of the radio frequency power. A slotted inner single loop single gap resonator (SLSG LGR), coaxial to the AGR, was used for signal detection. The resulting bimodal resonator (AG/LGR) has two mutually orthogonal magnetic field modes; one of them has the magnetic field in the axial direction. The resonator built in our laboratory achieved 40 dB isolation over 20 MHz bandwidth with quality factors of detection and excitation resonators of 36 and 11 respectively. Considerable improvement of the B1 homogeneity and EPR image quality in comparison with reflection loop-gap resonator of similar size and volume was observed., (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Published
- 2014
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20. A Dynamic Nuclear Polarization spectrometer at 95 GHz/144 MHz with EPR and NMR excitation and detection capabilities.
- Author
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Feintuch A, Shimon D, Hovav Y, Banerjee D, Kaminker I, Lipkin Y, Zibzener K, Epel B, Vega S, and Goldfarb D
- Subjects
- Cold Temperature, Electromagnetic Fields, Electron Spin Resonance Spectroscopy instrumentation, Magnetic Resonance Spectroscopy instrumentation, Microwaves, Signal Processing, Computer-Assisted, Spectrum Analysis, Electron Spin Resonance Spectroscopy methods, Magnetic Resonance Spectroscopy methods
- Abstract
A spectrometer specifically designed for systematic studies of the spin dynamics underlying Dynamic Nuclear Polarization (DNP) in solids at low temperatures is described. The spectrometer functions as a fully operational NMR spectrometer (144 MHz) and pulse EPR spectrometer (95 GHz) with a microwave (MW) power of up to 300 mW at the sample position, generating a MW B(1) field as high as 800 KHz. The combined NMR/EPR probe comprises of an open-structure horn-reflector configuration that functions as a low Q EPR cavity and an RF coil that can accommodate a 30-50 μl sample tube. The performance of the spectrometer is demonstrated through some basic pulsed EPR experiments, such as echo-detected EPR, saturation recovery and nutation measurements, that enable quantification of the actual intensity of MW irradiation at the position of the sample. In addition, DNP enhanced NMR signals of samples containing TEMPO and trityl are followed as a function of the MW frequency. Buildup curves of the nuclear polarization are recorded as a function of the microwave irradiation time period at different temperatures and for different MW powers., (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Published
- 2011
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21. Simultaneous acquisition of pulse EPR orientation selective spectra.
- Author
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Kaminker I, Florent M, Epel B, and Goldfarb D
- Subjects
- Computer Simulation, Spin Labels, Algorithms, Electron Spin Resonance Spectroscopy methods, Models, Chemical, Signal Processing, Computer-Assisted
- Abstract
High resolution pulse EPR methods are usually applied to resolve weak magnetic electron-nuclear or electron-electron interactions that are otherwise unresolved in the EPR spectrum. Complete information regarding different magnetic interactions, namely, principal components and orientation of principal axis system with respect to the molecular frame, can be derived from orientation selective pulsed EPR measurements that are performed at different magnetic field positions within the inhomogeneously broadened EPR spectrum. These experiments are usually carried out consecutively, namely a particular field position is chosen, data are accumulated until the signal to noise ratio is satisfactory, and then the next field position is chosen and data are accumulated. Here we present a new approach for data acquisition of pulsed EPR experiments referred to as parallel acquisition. It is applicable when the spectral width is much broader than the excitation bandwidth of the applied pulse sequence and it is particularly useful for orientation selective pulse EPR experiments. In this approach several pulse EPR measurements are performed within the waiting (repetition) time between consecutive pulse sequences during which spin lattice relaxation takes place. This is achieved by rapidly changing the main magnetic field, B(0), to different values within the EPR spectrum, performing the same experiment on the otherwise idle spins. This scheme represents an efficient utilization of the spectrometer and provides the same spectral information in a shorter time. This approach is demonstrated on W-band orientation selective electron-nuclear double resonance (ENDOR), electron spin echo envelope modulation (ESEEM), electron-electron double resonance (ELDOR)--detected NMR and double electron-electron resonance (DEER) measurements on frozen solutions of nitroxides. We show that a factors of 3-6 reduction in total acquisition time can be obtained, depending on the experiment applied., (Copyright © 2010 Elsevier Inc. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
22. HYSCORE and DEER with an upgraded 95GHz pulse EPR spectrometer.
- Author
-
Goldfarb D, Lipkin Y, Potapov A, Gorodetsky Y, Epel B, Raitsimring AM, Radoul M, and Kaminker I
- Subjects
- Equipment Design, Equipment Failure Analysis, Reproducibility of Results, Sensitivity and Specificity, Electromagnetic Phenomena instrumentation, Electron Spin Resonance Spectroscopy instrumentation, Microwaves, Signal Processing, Computer-Assisted instrumentation
- Abstract
The set-up of a new microwave bridge for a 95 GHz pulse EPR spectrometer is described. The virtues of the bridge are its simple and flexible design and its relatively high output power (0.7 W) that generates pi pulses of 25 ns and a microwave field, B(1)=0.71 mT. Such a high B(1) enhances considerably the sensitivity of high field double electron-electron resonance (DEER) measurements for distance determination, as we demonstrate on a nitroxide biradical with an interspin distance of 3.6 nm. Moreover, it allowed us to carry out HYSCORE (hyperfine sublevel-correlation) experiments at 95 GHz, observing nuclear modulation frequencies of 14N and 17O as high as 40 MHz. This opens a new window for the observation of relatively large hyperfine couplings, yet not resolved in the EPR spectrum, that are difficult to observe with HYSCORE carried out at conventional X-band frequencies. The correlations provided by the HYSCORE spectra are most important for signal assignment, and the improved resolution due to the two dimensional character of the experiment provides 14N quadrupolar splittings.
- Published
- 2008
- Full Text
- View/download PDF
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