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We present a case study of a long-term integrated monitoring of a flood event which affected part of the Strymonas dammed river basin, a transboundary river with source in Bulgaria, which flows then through Greece to the Aegean Sea. The event, which affected the floodplain downstream the Kerkini dam, started at the beginning of April 2015, due to heavy rain upstream of the monitored area, and lasted for several months, with some water pools still present at the beginning of September, due to the peculiar geomorphological conditions of the watershed. We collected a multi-temporal dataset consisting of a high-resolution, X-band COSMO-SkyMed, and several C-band Sentinel-1 SAR and optical Landsat-8 images of the area. The results allow following the event in time, sketching a multi-temporal map of the post-flood evolution, with relatively high temporal resolution. We then use hydrological modeling to mimic the dynamics of the flooded area against post event weather patterns and thus explain the observed flood extent evolution. We show how integrating remote sensing-derived maps of flooded areas, geomorphological analyses of the landscape and simplified hydrological modeling allows accurate inference about long-term dynamics of flooded areas, very important in the post event in anthropogenic highly modified areas, where recovery time after the flood event is considerable, and long term water persistence may lead to large consequences, carrying economic damages and medical emergencies., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Background and Aims: Augmenter of Liver Regeneration is a protein encoded by the Growth Factor Erv1-Like gene. Its biological properties are crucial for cell survival since knock-out mice for Growth Factor Erv1-Like gene do not survive. In this study, we injected hepatotropic adenoviral particles harboring oligonucleotide sequences against Growth Factor Erv1-Like gene into 70% partially hepatectomized rats and studied the effect of gene silencing on the progression liver regeneration., Methods: Partially hepatectomized rats were divided into three groups of animals and, before surgery, received either phosphate buffer saline, or adenoviral particles alone or adenoviral particles harboring the oligonucleotide silencing sequence. In each group, rats were sacrificed at 12, 24 and 48 h after surgery. Liver tissues were collected to analyze the expression of Augmenter of Liver Regeneration, Bax, Bcl-2 and activated Caspase-9 and -3, as well as hepatocyte proliferation and apoptosis, polyamines levels and histological and ultrastructural features., Results: Growth Factor Erv1-Like gene silencing reduced the compensatory hepatocellular proliferation triggered by surgery through (i) the reduction of polyamines synthesis, hepatocyte proliferation and anti-apoptotic gene expression and (ii) the increase of pro-apoptotic gene expression and caspase activation., Conclusions: For the first time, using a technique of gene silencing in vivo, our results demonstrate that Growth Factor Erv1-Like gene knock-down, i.e., the lack of Augmenter of Liver Regeneration, modifies the expression of genes involved in cell apoptosis and inhibits early phase of DNA synthesis. As a consequence, a promotion of cell death and a reduction of cell proliferation occurs., (Copyright © 2014 Elsevier Ltd. All rights reserved.)