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4 results on '"Fabrizio Palumbo"'

1. Optimized protocol for conditioned place avoidance learning in juvenile zebrafish

Author

Fabrizio Palumbo, Bram Serneels, and Emre Yaksi

Subjects
Model Organisms, Neuroscience, Behavior, Science (General), Q1-390
Abstract

Summary: Conditioned place avoidance assays are broadly used in mammals to study different cognitive aspects of operant learning. Here, we introduce a series of experimental designs for training juvenile zebrafish in short-term and long-term conditioned place avoidance assays. Our goal is to promote standardization of animal handling procedures and setup conditions to improve animal welfare and reproducibility while studying operant learning behaviors in juvenile zebrafish.For complete details on the use and execution of this protocol, please refer to Palumbo et al. (2020).

Published
2021
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2. The Zebrafish Dorsolateral Habenula Is Required for Updating Learned Behaviors

Author

Fabrizio Palumbo, Bram Serneels, Robbrecht Pelgrims, and Emre Yaksi

Subjects
habenula, zebrafish, conditioned place avoidance, learning, operant conditioning, reversal learning, Biology (General), QH301-705.5
Abstract

Summary: Operant learning requires multiple cognitive processes, such as learning, prediction of potential outcomes, and decision-making. It is less clear how interactions of these processes lead to the behavioral adaptations that allow animals to cope with a changing environment. We show that juvenile zebrafish can perform conditioned place avoidance learning, with improving performance across development. Ablation of the dorsolateral habenula (dlHb), a brain region involved in associative learning and prediction of outcomes, leads to an unexpected improvement in performance and delayed memory extinction. Interestingly, the control animals exhibit rapid adaptation to a changing learning rule, whereas dlHb-ablated animals fail to adapt. Altogether, our results show that the dlHb plays a central role in switching animals’ strategies while integrating new evidence with prior experience.

Published
2020
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3. Risk of unfavorable outcomes after penile prosthesis implantation – results from a national registry (INSIST-ED)

Author

M. Silvani, Antonio Vitarelli, M. Bitelli, P. Capogrosso, Nicola Mondaini, E. Pescatori, E. Conti, Giorgio Franco, C. Ceruti, F. Varvello, Antonio Avolio, E. Caraceni, Fabrizio Palumbo, S. Fiordelise, Antonella Palmieri, Giovanni Liguori, D. Pozza, Matteo Paradiso, Gabriele Antonini, A. Salonia, F. Colombo, L. Utizi, F. Dehò, and C. Bettocchi

Subjects
medicine.medical_specialty, business.industry, medicine.medical_treatment, General surgery, Urology, medicine, Penile prosthesis, National registry, business, lcsh:Diseases of the genitourinary system. Urology, lcsh:RC870-923, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, lcsh:RC254-282
Published
2020

4. Investigation of metabolite-protein interactions by transient absorption spectroscopy and in silico methods

Author

Miguel A. Miranda, Emilio Lence, Daniel Limones-Herrero, Fabrizio Palumbo, Victoria Vendrell-Criado, M. Consuelo Jiménez, Inmaculada Andreu, Concepción González-Bello, Ministerio de Economía, Industria y Competitividad (España), and Generalitat Valenciana

Subjects
Carprofen, Chlorpromazine, Stereochemistry, Metabolite, Carbazoles, Serum Albumin, Human, 02 engineering and technology, Molecular Dynamics Simulation, 010402 general chemistry, Methylation, 01 natural sciences, Analytical Chemistry, Docking, chemistry.chemical_compound, QUIMICA ORGANICA, Ultrafast laser spectroscopy, QUIMICA ANALITICA, medicine, Humans, Drug Interactions, Binding site, Instrumentation, Spectroscopy, Binding Sites, Hydrogen bond, Chemistry, Ligand, Molecular dynamics simulations, Anti-Inflammatory Agents, Non-Steroidal, Laser flash photolysis, Human serum albumin, Hydrogen Bonding, Stereoisomerism, 021001 nanoscience & nanotechnology, Atomic and Molecular Physics, and Optics, 0104 chemical sciences, Molecular Docking Simulation, Docking (molecular), Inactivation, Metabolic, Flash photolysis, Spectrophotometry, Ultraviolet, 0210 nano-technology, Antipsychotic Agents, Protein Binding, medicine.drug
Abstract

[EN] Transient absorption spectroscopy in combination with in silico methods has been employed to study the interactions between human serum albumin (HSA) and the anti-psychotic agent chlorpromazine (CPZ) as well as its two demethylated metabolites (MCPZ and DCPZ). Thus, solutions containing CPZ, MCPZ or DCPZ and HSA (molar ligand:protein ratios between 1:0 and 1:3) were submitted to laser flash photolysis and the Delta A(max) value at lambda = 470 nm, corresponding to the triplet excited state, was monitored. In all cases, the protein-bound ligand exhibited higher Delta Amax values measured after the laser pulse and were also considerably longer-lived than the non-complexed forms. This is in agreement with an enhanced hydrophilicity of the metabolites, due to the replacement of methyl groups with H that led to a lower extent of protein binding. For the three compounds, laser flash photolysis displacement experiments using warfarin or ibuprofen indicated Sudlow site I as the main binding site. Docking and molecular dynamics simulation studies revealed that the binding mode of the two demethylated ligands with HSA would be remarkable different from CPZ, specially for DCPZ, which appears to come from the different ability of their terminal ammonium groups to stablish hydrogen bonding interactions with the negatively charged residues within the protein pocket (Glu153, Glu292) as well as to allocate the methyl groups in an apolar environment. DCPZ would be rotated 180 degrees in relation to CPZ locating the aromatic ring away from the Sudlow site I of HSA. (C) 2019 Elsevier B.V. All rights reserved., Financial support from Ministerio de Economia, Industria y Competitividad (CTQ2016-78875-P, SAF2016-75638-R, BES-2011-043706), Generalitat Valenciana (Prometeo 2017/075), Xunta de Galicia [Centro Singular de Investigacion de Galicia accreditation 2016-2019 (ED431G/09, ED431B 2018/04) and post-doctoral fellowship to E. L.] and European Union (European Regional Development Fund-ERDF) is gratefully acknowledged. I. A. holds a "Miguel Servet" contract (CP1116/00052) funded by the Carlos III Health Institute. We are grateful to the Centro de Supercomputacion de Galicia (CESGA) for computational facilities.

Published
2020

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