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Liamocins, as the secondary metabolites synthesized and secreted by Aureobasidium spp., consist of a single mannitol or a single arabitol head group partially O-acylated with three 3,5-dihydroxydecanoic ester groups or directly esterified with three or four 3,5-dihydroxydecanoic ester tails. Very recently, the whole synthetic pathway of liamocins in A. melanogenum 6-1-2 has been elucidated. It was found that the promoter sequences of all the genes related to liamocin synthesis in A. melanogenum 6-1-2 had stress regulatory elements with core sequences of AGGGG or CCCCT. Therefore, expression of all the genes would be regulated by the Msn2. In this study, it was found that removal of the single one MSN2 gene in A. melanogenum 6-1-2 made the mutant decrease yield of extracellular liamocin by 92.28 %, while complementation of the MSN2 gene in the mutant rendered liamocin synthesis to be restored. When A. melanogenum 6-1-2 was cultured in the liamocin fermentation medium with high glucose and low nitrogen, the Msn2 was localized in the nucleus and positively regulated the expression of the genes related to liamocin biosynthesis. Furthermore, when the key BCY1 gene encoding regulatory subunit of the cAMP-PKA signaling pathway in A. melanogenum 6-1-2 was knocked out, the amount of extracellular liamocins synthesized by the mutant was decreased by 96.73 % and the Msn2 was localized in the cytoplasm. Similarly, when the key HOG1 gene in the HOG1 signaling pathway was deleted, liamocin biosynthesis in the knockout strain was decreased by 98.09 %. However, it was found that the Hog1 may be one part of the general transcription complex to regulate the transcription of the MSN2 gene, leading to the reduced Msn2 and liamocin synthesis in the mutant. In addition, the key TOR1 gene and SNF1 gene in the TOR1 signaling pathway and the SNF1 signaling pathway were not involved in the regulation of the Msn2 activity and liamocin synthesis. It was concluded that the transcriptional activator Msn2, the HOG1 signaling pathway and the cAMP-PKA signaling pathway were involved in the regulation of liamocin biosynthesis and production., (Copyright © 2020 Elsevier Inc. All rights reserved.)

This study compared the degradation of dissolved organic matter (DOM) by UV/chlorine advanced oxidation processes (AOPs) with emerging ultraviolet light-emitting diode (UV-LED, 275 nm) and traditional low pressure UV (LPUV, 254 nm) as UV sources. Excitation emission matrix-parallel factor (EEM-PARAFAC) analysis and two-dimensional (2D) correlation gel permeation chromatograph were applied to explore the evolutions of DOM during oxidation processes. The degradation behaviors of DOM indicated by UV absorbance at 254 nm (UV 254 ), dissolved organic carbon (DOC), and fluorophores fitted the pseudo-first-order kinetics well. The removal efficiency of DOM was similar under UV-LED and LPUV irradiation alone. However, UV-LED exhibited much higher degradation rates (increased by 29-160%) than LPUV regardless of the tracking variables during UV/chlorine processes. For three PARAFAC components, humic-like fluorescences were preferentially degraded by UV/chlorine oxidation compared with protein-like fluorescence potentially due to the differences of electronic moieties and molecular weight (MW). The decline in UV 254 , DOC, and fluorophores increased with increasing chlorine dosage; linear correlations between those indicators were observed during the two AOPs. Moreover, UV-LED/chlorine could achieve greater extents of MW change. Our study demonstrated that UV-LED could be a superior alternative for the future selection of UV source in the UV/chlorine process., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Pulsed ultraviolet (UV) irradiation has presented enhanced inactivation efficiency in water disinfection and food decontamination. As an emerging UV source, UV light-emitting diodes (UV-LEDs) are an attractive alternative for pulsed irradiation because they can be turned on and off with a high and adjustable frequency. In this study, disinfection efficiencies of pulsed and continuous UV-LED irradiation were compared for Escherichia coli (E. coli) inactivation in water using a high power 285 nm LED and low power 265 and 280 nm LEDs. Factors including various duty cycles, pulse frequencies and UV irradiances were evaluated. The log-inactivation of E. coli increased substantially as the duty cycle decreased from 100% to 5% at the same UV dose. For 265 and 280 nm LEDs, the log-inactivation enhancements of pulsed UV irradiation were similar. When a higher irradiance was applied, the energy efficiency enhancement of pulsed UV irradiation became more obvious. The log-inactivation of E. coli enhanced remarkably using high current pulsed irradiation of 280 nm LEDs. Compared to continuous UV irradiation, pulsed UV-LED irradiation is an attractive alternative for E. coli inactivation in water considering energy efficiency., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Chloramine disinfection is one of the most common disinfection methods in drinking water treatment. In this study, the temporal variability of water odors during monochloramine auto-decomposition was investigated to elucidate the characteristics of odor problems caused by adopting chloramine disinfection in tap water. Odor intensities and dominant odorant contributions were determined using the flavor profile analysis (FPA) and odor active value (OAV), respectively. During auto-decomposition of monochloramine, Cl 2 /N molar ratio, pH, temperature, and the presence of NOM all affected odor intensity and odor temporal variation in drinking water. In general, decreasing pH from 8.5 to 6.0 led to increasing perceived odor intensity due to the formation of dichloramine. The major odorants responsible for chlorinous odor under acidic and non-acidic conditions were dichloramine and monochloramine, respectively. Chloraminated water with a Cl 2 /N molar ratio of 0.6 or NOM concentration <2 mg-C L -1 inhibited odor intensity. Furthermore, the influence of rechlorination on chlorinous odor intensity for chloraminated water should not be neglected. The results of this study will be beneficial for the control of chlorinous odors caused by chloramine disinfection in drinking water., (Copyright © 2018 Elsevier B.V. All rights reserved.)

This study investigated the formation of toxic iodinated trihalomethanes (I-THMs) during breakpoint chlorination of iodide-containing water. Impact factors including I - concentration, natural organic matter (NOM) concentration and type, pH as well as Br - /I - molar ratio were systematically investigated. Moreover, the incorporation of I - into I-THM formation was also calculated. The results showed that I-THM formation varied in different zones of the breakpoint curves. I-THMs increased with increasing chlorine dosage to breakpoint value and then dropped significantly beyond it. Iodoform (CHI 3 ) and chlorodiiodomethane (CHClI 2 ) were the major I-THMs in the pre-breakpoint zone, while dichloroiodomethane (CHCl 2 I) was the dominant one in the post-breakpoint zone. The formation of I-THMs increased remarkably with I - and dissolved organic carbon (DOC) concentrations. More bromine-containing species were formed as Br - /I - molar ratio increased from 0.5 to 5. In addition, the major I-THM compound shifted from CHCl 2 I to the more toxic CHClBrI. As pH increased from 6.0 to 8.0, I-THM formation kept increasing in the pre-breakpoint zone and the speciation of I-THMs changed alongside the breakpoint curves. The incorporation of I - during breakpoint chlorination was highly dependent on chlorine, I - , and NOM concentrations, NOM type, solution pH and Br - /I - molar ratio., (Copyright © 2018 Elsevier B.V. All rights reserved.)