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Your search keyword '"Hao, Chun-Yan"' showing total 2 results
Start Over Author "Hao, Chun-Yan" Publisher elsevier
2 results on '"Hao, Chun-Yan"'

1. Developmental potential of oocytes fertilized by conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) after cryopreservation and mesometrial autotransplantation of rabbit ovarian tissue

Author

Deng Xiaohui, Hao Chun-yan, X. Anran, F. Qingzhao, Y. Xuan, Yu Hong-Ling, and Chao Lan

Subjects
In vitro fertilisation, urogenital system, medicine.medical_treatment, Biology, follicle development, Oocyte, SF1-1100, Cryopreservation, Intracytoplasmic sperm injection, Animal culture, Transplantation, Andrology, Pregnancy rate, medicine.anatomical_structure, Human fertilization, oocyte maturation, in vitrofertilization, embryonic structures, medicine, Animal Science and Zoology, Blastocyst, reproductive and urinary physiology, ovarian tissue, transplantation
Abstract

Objective: To evaluate mesometrial transplantation of frozen-thawed ovarian tissue in rabbit and to choose the optimized fertilization method for oocytes retrieved from grafts by investigating the capability of oocyte fertilization and further development. Forty rabbits were divided into three groups randomly: control group, fresh tissues transplantation group and frozen-thawed tissues transplantation group. Three months after the transplantation, rabbits were stimulated with FSH and oocytes were retrieved 13 h after human chorionic gonadotropin (HCG) injection. Oocytes matured in vivo or in vitro were then fertilized by conventional in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI), followed by observation and evaluation of fertilization rate and blastocyst formation rate. Blastocytes embryos were transferred to pseudopregnancy rabbits to observe pregnancy rate and birth rate. There were no significant differences in the percentage of oocytes matured either in vivo or in vitro among the three groups. The fertilization rate, cleavage rate and blastocyst formation rate of in vivo-matured oocytes had no difference among the three groups, whether they were fertilized by IVF or ICSI. Significantly higher fertilization rates of in vitro-matured oocytes were observed with ICSI compared with IVF in each group. The blastocyst formation rate of in vitro-matured oocytes was significantly lower than that of in vivo-matured oocytes in each group. The birth rate of in vivo-matured oocytes was significantly higher than that of in vitro-matured oocytes, although the pregnancy rate was similar between them. Mesometrial transplantation of frozen-thawed ovarian tissue may provide favorable conditions for follicle development. Oocytes retrieved from mesometrial grafts can develop to the blastocyst stage and produce live offspring. ICSI can optimize the fertilization rate of in vitro-matured oocytes retrieved from grafts.

Published
2008

2. Arsenic trioxide overcomes apoptosis inhibition in K562/ADM cells by regulating vital components in apoptotic pathway.

Author

Wang DH, Wei HL, Zhao HS, Hao CY, Min ZH, and Liu JM

Subjects
Arsenic Trioxide, Caspase 3, Caspases genetics, Cell Cycle drug effects, Cell Proliferation drug effects, DNA Fragmentation drug effects, Drug Resistance, Multiple, Drug Resistance, Neoplasm, Genes, MDR, Humans, Proto-Oncogene Proteins c-bcl-2 analysis, Tumor Suppressor Protein p53 analysis, Antineoplastic Agents pharmacology, Apoptosis drug effects, Arsenicals pharmacology, Doxorubicin pharmacology, K562 Cells drug effects, Oxides pharmacology
Abstract

Extensive researches have revealed that arsenical can exert anti-tumor efficacy against several kinds of cancers including leukemia. Though, little is known about the effects of arsenical on leukemia resistant to chemotherapy, emerging as a serious clinical problem. In this study, we tested arsenic trioxide (As(2)O(3))-induced apoptosis in K562/ADM multidrug-resistant leukemic cells and investigated its possible mechanisms. Using microscopy, flow cytometry (FCM) and DNA electrophoresis, we found that As(2)O(3) could induce the cells to undergo G2/M phase arrest and apoptosis. Further, it was shown that the levels of FAS and P53 proteins increased and P-glycoprotein (P-gp) decreased upon drug action by employing FCM. Reverse transcription polymerase chain reaction (RT-PCR) detected increased mRNA product of FAS and caspase-3 genes and reduced MDR1 mRNA. CASPASE-3 activity was also enhanced after As(2)O(3) treatment. However, the expression of BCL-2 protein was not affected by the drug. Taken together, As(2)O(3) is able to reverse the apoptosis resistance in drug-resistant K562/ADM cells by modulating expression or activity of key factors associated with apoptosis induction.

Published
2005
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