21 results on '"Herzog B"'
Search Results
2. Usefulness of additional coronary calcium scoring in low-dose CT coronary angiography with prospective ECG-triggering impact on total effective radiation dose and diagnostic accuracy
- Author
-
Husmann, L, Herzog, B A, Burger, I A, Buechel, R R, Pazhenkottil, A P, von Schulthess, P, Wyss, C A, Gaemperli, O, Landmesser, U, Kaufmann, P A, University of Zurich, and Kaufmann, P A
- Subjects
10076 Center for Integrative Human Physiology ,10209 Clinic for Cardiology ,570 Life sciences ,biology ,2741 Radiology, Nuclear Medicine and Imaging ,610 Medicine & health ,10181 Clinic for Nuclear Medicine - Published
- 2010
- Full Text
- View/download PDF
3. Comparative immunohistochemical staining of atherosclerotic plaques using F16, F8 and L19: Three clinical-grade fully human antibodies
- Author
-
Pedretti, M, Rancic, Z, Soltermann, A, Herzog, B A, Schliemann, C, Lachat, Mario, Neri, D, Kaufmann, P A, Pedretti, M, Rancic, Z, Soltermann, A, Herzog, B A, Schliemann, C, Lachat, Mario, Neri, D, and Kaufmann, P A
- Abstract
OBJECTIVE: F16, F8 and L19 are three fully human monoclonal antibodies, specific to splice isoforms of tenascin-C and fibronectin, which stain sites of active tissue remodeling and which are currently in Phase I and II clinical trials as radio-immunoconjugates and immunocytokines in patients with cancer and arthritis. The characterization of atherosclerosis using these antibodies may open novel pharmacodelivery options for the imaging and treatment of cardiovascular conditions. It may also allow a better assessment of the corresponding immunoconjugates in polymorbid patients with atherosclerotic plaques. METHODS: We performed a comparative immunohistochemical analysis with the F16, F8 and L19 antibodies in 28 freshly frozen human carotid plaques and in 11 normal arteries. Furthermore, we assessed the localization of the antibodies in relation to the infiltrating macrophages, vasa vasorum and Ki67-positive proliferating cells of the plaque. RESULTS: The F16 antibody, specific to the extra-domain A1 of tenascin-C, stained plaques with a selective and intense pattern, while F8 and L19, specific to the EDA and EDB domains of fibronectin, respectively, exhibited a less selective and intense staining. In immunofluorescence, F16 was found to bind regions rich in macrophages, vasa vasorum and proliferating cells, while showing no detectable vs. weak staining of normal arteries and of quiescent plaque structures. CONCLUSION: The human monoclonal antibody F16 stains areas of active tissue remodeling in atherosclerotic plaques and may thus deserve to be investigated as a suitable building block for the development of radiopharmaceuticals for plaque imaging or for the antibody-based targeted delivery of therapeutic agents to atherosclerotic lesions.
- Published
- 2010
4. Visual and quantitative perfusion analysis in left main stem disease: a CE-MARC substudy
- Author
-
Greenwood John P, Kidambi Ananth, Maredia Neil, Mohee Kevin, Sourbron Steven, Motwani Manish, Uddin Akhlaque, Ripley David P, Herzog Bernhard A, Zaman Arshad, Dickinson Catherine J, Brown Julia, Nixon Jane, Everett Colin, and Plein Sven
- Subjects
Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Published
- 2013
- Full Text
- View/download PDF
5. Quantitative analysis of post-TAVI aortic regurgitation with cardiovascular magnetic resonance and the relationship to transthoracic echocardiography
- Author
-
Uddin Akhlaque, Fairbairn Timothy, Steadman Christopher D, Herzog Bernhard A, Motwani Manish, Kidambi Ananth, Schlosshan Dominik, Blackman Daniel, McCann Gerry P, Plein Sven, and Greenwood John P
- Subjects
Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Published
- 2013
- Full Text
- View/download PDF
6. The ischaemic and scar burden measured by cardiac magnetic resonance imaging in patients with ischaemic coronary heart disease from the CE-MARC study
- Author
-
Plein Sven, Herzog Bernhard A, Maredia Neil, Kidambi Ananth, Motwani Manish, Uddin Akhlaque, Ripley David P, Dickinson Catherine J, Brown Julia, Nixon Jane, Everett Colin, and Greenwood John P
- Subjects
Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Published
- 2013
- Full Text
- View/download PDF
7. Prognosis of anomalous coronary arteries originating from the opposite sinus of Valsalva (ACAOS): 15 year experience from two large CMR centres
- Author
-
Ripley David P, Teis Albert, Uddin Akhlaque, Bijsterveld Petra, Saha Ansuman, Kidambi Ananth, Herzog Bernhard A, Plein Sven, Pennell Dudley J, and Greenwood John P
- Subjects
Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Published
- 2013
- Full Text
- View/download PDF
8. Reciprocal ECG change in ST-elevation myocardial infarction is associated with area at risk and myocardial salvage following revascularization
- Author
-
Kidambi Ananth, Mather Adam N, Uddin Akhlaque, Motwani Manish, Ripley David P, Herzog Bernhard A, Gunn Julian, Plein Sven, and Greenwood John P
- Subjects
Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Published
- 2013
- Full Text
- View/download PDF
9. Myocardial bridging in patients with hypertrophic cardiomyopathy is not associated with late gadolinium enhancement at cardiac magnetic resonance imaging
- Author
-
Hees Tilman A, Herzog Benjamin, Kristen Arnt, Katus Hugo A, and Abdel-Aty Hassan
- Subjects
Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Published
- 2013
- Full Text
- View/download PDF
10. Associated factors for a false negative cardiovascular magnetic resonance perfusion study: a CE-MARC substudy
- Author
-
Plein Sven, Kidambi Ananth, Sourbron Steven, Maredia Neil, Uddin Akhlaque, Motwani Manish, Ripley David P, Herzog Bernhard A, Brown Julia, Nixon Jane, Everett Colin, and Greenwood John P
- Subjects
Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Published
- 2013
- Full Text
- View/download PDF
11. A global multinational survey of cefotaxime-resistant coliforms in urban wastewater treatment plants
- Author
-
Yunho Lee, Manika Choudhury, Om Prakash, Donald Morrison, Hélène Guilloteau, Heidrun Mayrhofer, María Inmaculada Polo-López, Belen Esteban, Katarzyna Slipko, Leonardo Pantoja Munoz, Samira Nahim–Granados, José Manuel Guillén-Navarro, Marco Guida, Stela Krizanovic, Hemda Garelick, Pilar Caballero, Traore Ousmane, Marcos Quintela-Baluja, Sara Rodríguez-Mozaz, Helmut Bürgmann, Nikolina Udiković-Kolić, Carsten Ulrich Schwermer, Agnieszka Kalinowska, Milena Milaković, Ester Heath, Marta Piotrowska, Andrea Di Cesare, Célia M. Manaia, Ying Yang, Amy Pruden, Anne F.C. Leonard, Erica Donner, Tanel Telson, Christophe Merlin, Karin Beck, Ana Agüera, Anhony A Adegoke, Marie-Noëlle Pons, Bastian Herzog, Joana Abreu-Silva, Leonie Henn, Norbert Kreuzinger, Younggun Yoon, Stefanie Heß, Olga C. Nunes, Alice L. Petre, Despo Fatta-Kassinos, Gianluca Brunetti, Roberto B. M. Marano, Stella Michael, Thor A. Stenström, Ayella Maile-Moskowitz, Popi Karaolia, Joshua T. Bunce, Giovanni Libralato, Jérôme Ory, Yogesh Nimonkar, Alfieri Pollice, Aneta Luczkiewicz, Carlo Salerno, Andrew Scott, Shichun Zou, Antoni Oliver, Barbara Drigo, Isabel Martínez-Alcalá, Virginia Riquelme, Veljo Kisand, Anna Baraniak, William H. Gaze, Edward Topp, Edouard Jurkevitch, Isabel Henriques, Telma Fernandes, Eddie Cytryn, José Luis Balcázar, Marta Tacão, Thomas Schwartz, Thomas Jäger, Gianluca Corno, Mailis Laht, Thomas U. Berendonk, Roberto Rosal, Magdalena Popowska, Pawel Krzeminski, Connor L. Brown, Veritati - Repositório Institucional da Universidade Católica Portuguesa, Marano, R. B. M., Fernandes, T., Manaia, C. M., Nunes, O., Morrison, D., Berendonk, T. U., Kreuzinger, N., Telson, T., Corno, G., Fatta-Kassinos, D., Merlin, C., Topp, E., Jurkevitch, E., Henn, L., Scott, A., Hess, S., Slipko, K., Laht, M., Kisand, V., Di Cesare, A., Karaolia, P., Michael, S. G., Petre, A. L., Rosal, R., Pruden, A., Riquelme, V., Aguera, A., Esteban, B., Luczkiewicz, A., Kalinowska, A., Leonard, A., Gaze, W. H., Adegoke, A. A., Stenstrom, T. A., Pollice, A., Salerno, C., Schwermer, C. U., Krzeminski, P., Guilloteau, H., Donner, E., Drigo, B., Libralato, G., Guida, M., Burgmann, H., Beck, K., Garelick, H., Tacao, M., Henriques, I., Martinez-Alcala, I., Guillen-Navarro, J. M., Popowska, M., Piotrowska, M., Quintela-Baluja, M., Bunce, J. T., Polo-Lopez, M. I., Nahim-Granados, S., Pons, M. -N., Milakovic, M., Udikovic-Kolic, N., Ory, J., Ousmane, T., Caballero, P., Oliver, A., Rodriguez-Mozaz, S., Balcazar, J. L., Jager, T., Schwartz, T., Yang, Y., Zou, S., Lee, Y., Yoon, Y., Herzog, B., Mayrhofer, H., Prakash, O., Nimonkar, Y., Heath, E., Baraniak, A., Abreu-Silva, J., Choudhury, M., Munoz, L. P., Krizanovic, S., Brunetti, G., Maile-Moskowitz, A., Brown, C., Cytryn, E., The Robert H. Smith Faculty of Agriculture, Food and Environment, Department of Animal Sciences, The Hebrew University of Jerusalem (HUJ), Institute of Soil, Water and Environmental Sciences, Agricultural Research Organization - the Volcani Center, Centro de Química Fina e Biotecnologia (CQFB), Requimte, Universidade do Porto-Departamento de Química (DQ), Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Faculdade de Ciências e Tecnologia = School of Science & Technology (FCT NOVA), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA), Laboratory for Process Engineering, Environment, Biotechnology and Energy (LEPABE), Universidade do Porto, Edinburgh Napier University, Institute for Hydrobiology, Technische Universität Dresden = Dresden University of Technology (TU Dresden), Vienna University of Technology, Institute of Technology [Tartu, Estonia], University of Tartu, CNR Water Research Institute (IRSA), Consiglio Nazionale delle Ricerche (CNR), University of Cyprus (UCY), Laboratoire de Chimie Physique et Microbiologie pour les Matériaux et l'Environnement (LCPME), Institut de Chimie du CNRS (INC)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Agriculture and Agri-Food [Ottawa] (AAFC), Department of Biology, University of Western Ontario, University of Western Ontario (UWO), Estonian Environmental Research Centre (EKUK), Universidad de Alcalá - University of Alcalá (UAH), Virginia Tech [Blacksburg], Area de Quimica Inorganica - Centro de Investigacion en Energia Solar (CIESOL) (CIESOL), Universidad de Almería (UAL), Gdańsk University of Technology (GUT), University of Exeter Medical School, University of Exeter, Durban University of Technology, Universidad Nacional de Cuyo [Mendoza] (UNCUYO), Norwegian Institute for Water Research (NIVA), University of South Australia [Adelaide], Università degli studi di Napoli Federico II, Swiss Federal Insitute of Aquatic Science and Technology [Dübendorf] (EAWAG), Middlesex University [London], Universidade de Aveiro, Universidade de Coimbra [Coimbra], Universidad Católica San Antonio de Murcia (UCAM), Department of Applied Microbiology [Warsaw], Institute of Microbiology [Warsaw], Faculty of Biology [Warsaw], University of Warsaw (UW)-University of Warsaw (UW)-Faculty of Biology [Warsaw], University of Warsaw (UW)-University of Warsaw (UW), School of Engineering [Newcastle], Newcastle University [Newcastle], Plataforma Solar de Almeria – CIEMAT, Tabernas, Almeria, Laboratoire Réactions et Génie des Procédés (LRGP), Centre National de la Recherche Scientifique (CNRS)-Université de Lorraine (UL), Rudjer Boskovic Institute [Zagreb], Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), CHU Clermont-Ferrand, Laboratorio EMATSA, Instituto Catalán de Investigación del Agua - ICRA (SPAIN) (ICRA), Institute of Functional Interfaces, Karlsruhe Institute of Technology (KIT), Sun Yat-Sen University [Guangzhou] (SYSU), Gwangju Institute of Science and Technology (GIST), Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), NCMR, Jozef Stefan Institute [Ljubljana] (IJS), National Medicines Institute - Narodowy Instytut Leków [Warsaw] (NIL), Agricultural Research Organisation (ARO), Volcani Center, Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade Nova de Lisboa = NOVA University Lisbon (NOVA)-Universidade do Porto = University of Porto-Departamento de Química (DQ), Universidade do Porto = University of Porto, National Research Council of Italy | Consiglio Nazionale delle Ricerche (CNR), University of Cyprus [Nicosia] (UCY), Agriculture and Agri-Food (AAFC), University of Naples Federico II = Università degli studi di Napoli Federico II, Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS), Marano, Robert BM, Fernandes, Telma, Manaia, Celia M, Nunes, Olga, Donner, Erica, Drigo, Barbara, Brunetti, Gianluca, and Cytryn, Eddie
- Subjects
Cefotaxime ,010504 meteorology & atmospheric sciences ,Coliforms ,Antibiotic resistance ,Sewage ,Microorganismes -- Resistència als medicaments ,Wastewater treatment ,Wastewater ,010501 environmental sciences ,01 natural sciences ,Surveys and Questionnaires ,Microorganisms -- Drug resistance ,ComputingMilieux_MISCELLANEOUS ,lcsh:Environmental sciences ,General Environmental Science ,lcsh:GE1-350 ,6. Clean water ,Anti-Bacterial Agents ,3. Good health ,Europe ,Interdisciplinary Natural Sciences ,Health ,Sewage treatment ,Biotechnology ,medicine.drug ,Life sciences ,biology ,Aigua -- Reutilització ,Asia ,Quantification methods ,Aigües residuals -- Plantes de tractament ,Culture and Communities ,Water Purification ,Applied microbiology ,Water reuse ,Environmental health ,ddc:570 ,medicine ,Microbiology Research Group ,[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering ,antibiotic resistance ,coliforms ,ESBLs ,wastewater treatment ,wastewater reuse ,Sewage disposal plants ,Effluent ,0105 earth and related environmental sciences ,[SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health ,Coliform ,business.industry ,Australia ,Fecal coliform ,Antibiotic resistance, Coliforms, ESBLs, Wastewater treatment, Water reuse ,ESBL ,North America ,Environmental science ,business - Abstract
The World Health Organization Global Action Plan recommends integrated surveillance programs as crucial strategies for monitoring antibiotic resistance. Although several national surveillance programs are in place for clinical and veterinary settings, no such schemes exist for monitoring antibiotic-resistant bacteria in the environment. In this transnational study, we developed, validated, and tested a low-cost surveillance and easy to implement approach to evaluate antibiotic resistance in wastewater treatment plants (WWTPs) by targeting cefotaxime-resistant (CTX-R) coliforms as indicators. The rationale for this approach was: i) coliform quantification methods are internationally accepted as indicators of fecal contamination in recreational waters and are therefore routinely applied in analytical labs; ii) CTX-R coliforms are clinically relevant, associated with extended-spectrum β-lactamases (ESBLs), and are rare in pristine environments. We analyzed 57 WWTPs in 22 countries across Europe, Asia, Africa, Australia, and North America. CTX-R coliforms were ubiquitous in raw sewage and their relative abundance varied significantly (
- Published
- 2020
12. Use of phosphodiesterase-5 inhibitors and the incidence of melanoma.
- Author
-
Wayne G, Demus T, Jivanji D, Atri E, Herzog B, Wong V, Garcia M, Cedeno J, Nagoda E, and Polackwich A
- Abstract
Introduction: Recent evidence of a causal link between Phosphodiesterase-5-inhibitor (PDE-5i) use and melanoma has caused concern in PDE-5i use and was even addressed in the 2018 American Urological Association guideline on erectile dysfunction (ED). Given that several studies have affirmed this low probability but statistically significant association, one might expect a shift in melanoma diagnoses since PDE-5is were introduced in 1998. We sought to determine if the introduction of PDE-5i drugs for ED treatment increased incidence of melanoma., Methods: The Surveillance, Epidemiology, and End Results (SEER) database was used to compare the incidence of melanoma diagnosis in American men between 1973 and 2015, providing over a decade of data before and after PDE-5i introduction in 1998. Interrupted time-series and logistic regression were used to assess this relationship., Results: Over 43 years, the SEER database has reported 292,166 cases of Melanoma, with males accounting for 53.7% of cases (Standard deviation [SD] 3%, Range 47.5-58.3%). After the introduction of PDE-5i, there was no proportional increase in melanoma diagnoses, in fact demonstrating a 2% lower incidence from prediction models (p < 0.05)., Conclusion: Our analysis of the SEER database demonstrates that the trend in incidence of melanoma has fallen in the era of PDE-5i use for ED. These findings may be of value in counseling patients anxious about the potential association between PDE-5i use and skin cancer; however, continued research analyzing individual-level risk are needed., Competing Interests: Declaration of Competing Interest All authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2022
- Full Text
- View/download PDF
13. Crystallization Velocity and UV Performance of Formulations With Oversaturated UV-Filter Content.
- Author
-
Sohn M, Prost-Dame M, Bayraktar M, Schäfer A, and Herzog B
- Subjects
- Chemistry, Pharmaceutical methods, Crystallization methods, Emulsions chemistry, Oils chemistry, Skin drug effects, Water chemistry, Sunscreening Agents chemistry, Ultraviolet Rays adverse effects
- Abstract
Cosmetic oils are used to dissolve crystalline lipophilic UV filters; however, little knowledge exists about the effect of other formulation ingredients. This study investigates the influence of emulsifiers on the recrystallization speed of 4 UV filters and the impact of UV-filter crystal formation on delivered performance. The crystallization pattern of studied UV filters was assessed using X-ray diffractometry, whereas their recrystallization speed in formulations with various emulsifiers was monitored microscopically. UV-filter concentration was above the saturation level to promote recrystallization. Furthermore, to understand the kinetics of recrystallization, the conformer number of each UV-filter was calculated. For the impact on performance, the absorbance of a sunscreen was measured before and after recrystallization of the contained UV filter. This study confirmed the crystallinity of tested UV filters. The emulsifier was shown to influence the UV-filter recrystallization speed in emulsions. Continuous oil phase sunscreens were critical; all UV filters recrystallized promptly in oils and water-in-oil emulsions. Large molecule UV filters showed slowest recrystallization speed explained by a higher number of possible conformers. Finally, this work confirmed the negative impact of crystal formation on the delivered photoprotection of a sunscreen., (Copyright © 2019 American Pharmacists Association®. Published by Elsevier Inc. All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
14. Liposomogenic UV Absorbers are Water-Resistant on Pig Skin-A Model Study With Relevance for Sunscreens.
- Author
-
Herzog B, Hüglin D, and Luther H
- Subjects
- Animals, Lipid Bilayers chemistry, Lipid Bilayers metabolism, Liposomes metabolism, Phosphatidylcholines metabolism, Skin metabolism, Skin Absorption, Sunscreening Agents administration & dosage, Sunscreening Agents pharmacokinetics, Swine, Ultraviolet Rays, Water metabolism, Liposomes chemistry, Phosphatidylcholines chemistry, Sunscreening Agents chemistry, Water chemistry
- Abstract
An important property of sunscreens is their water resistance after the application on human skin. In this work, the hypothesis that UV absorber molecules which are able to form liposomes, so-called liposomogenic UV absorbers, show better water resistance on a pig skin model than UV-absorbing molecules lacking this ability was tested. The assumption behind is that molecules which can form liposomes are able to integrate into the stratum corneum lipids of the skin. Three different liposomogenic UV absorbers were synthesized and their behavior investigated, leading to the confirmation of the hypothesis. With one of the liposomogenic UV absorbers, it was possible to show the integration of the UV absorber molecules into the bilayers of another liposome consisting of phosphatidylcholine, supporting the assumption that liposomogenic UV absorbers exhibit improved water resistance because they integrate into the skin lipids., (Copyright © 2017 American Pharmacists Association®. Published by Elsevier Inc. All rights reserved.)
- Published
- 2017
- Full Text
- View/download PDF
15. Podoplanin requires sialylated O-glycans for stable expression on lymphatic endothelial cells and for interaction with platelets.
- Author
-
Pan Y, Yago T, Fu J, Herzog B, McDaniel JM, Mehta-D'Souza P, Cai X, Ruan C, McEver RP, West C, Dai K, Chen H, and Xia L
- Subjects
- Animals, Blood Platelets cytology, CHO Cells, Cell Communication drug effects, Cricetulus, Dipeptides pharmacology, Endothelial Cells cytology, Gene Expression Regulation drug effects, Glycosylation drug effects, Matrix Metalloproteinase 2 biosynthesis, Matrix Metalloproteinase 2 genetics, Matrix Metalloproteinase 9 biosynthesis, Matrix Metalloproteinase 9 genetics, Matrix Metalloproteinase Inhibitors pharmacology, Membrane Glycoproteins genetics, Mice, Mice, Knockout, Platelet Adhesiveness drug effects, Platelet Adhesiveness physiology, Polysaccharides genetics, Sialic Acids genetics, Sialic Acids metabolism, Blood Platelets metabolism, Cell Communication physiology, Endothelial Cells metabolism, Gene Expression Regulation physiology, Membrane Glycoproteins metabolism, Polysaccharides biosynthesis
- Abstract
O-glycosylation of podoplanin (PDPN) on lymphatic endothelial cells is critical for the separation of blood and lymphatic systems by interacting with platelet C-type lectin-like receptor 2 during development. However, how O-glycosylation controls endothelial PDPN function and expression remains unclear. In this study, we report that core 1 O-glycan-deficient or desialylated PDPN was highly susceptible to proteolytic degradation by various proteases, including metalloproteinases (MMP)-2/9. We found that the lymph contained activated MMP-2/9 and incubation of the lymph reduced surface levels of PDPN on core 1 O-glycan-deficient endothelial cells, but not on wild-type ECs. The lymph from mice with sepsis induced by cecal ligation and puncture, which contained bacteria-derived sialidase, reduced PDPN levels on wild-type ECs. The MMP inhibitor, GM6001, rescued these reductions. Additionally, GM6001 treatment rescued the reduction of PDPN level on lymphatic endothelial cells in mice lacking endothelial core 1 O-glycan or cecal ligation and puncture-treated mice. Furthermore, core 1 O-glycan-deficient or desialylated PDPN impaired platelet interaction under physiological flow. These data indicate that sialylated O-glycans of PDPN are essential for platelet adhesion and prevent PDPN from proteolytic degradation primarily mediated by MMPs in the lymph., (© 2014 by The American Society of Hematology.)
- Published
- 2014
- Full Text
- View/download PDF
16. Determination of optimal conditions for 5-methyl-benzotriazole biodegradation with activated sludge communities by dilution of the inoculum.
- Author
-
Yuan H, Herzog B, Helmreich B, Lemmer H, and Müller E
- Subjects
- Biodegradation, Environmental, Bioreactors microbiology, Sewage chemistry, Triazoles analysis, Water Pollutants, Chemical analysis, Sewage microbiology, Triazoles metabolism, Waste Disposal, Fluid methods, Water Pollutants, Chemical metabolism
- Abstract
The aerobic biodegradation of 5-methyl-benzotriazole (5-TTri) was optimized using lab-scale setups and activated sludge communities (ASC) collected from three wastewater treatment plants (WWTP) MBR-MH, CAS-E and CAS-M being different in their treatment technologies. ASC inocula were diluted to rule out non-biodegrading species and incubated under two nutrient conditions: A) mineral salt media (MSM) and B) carbon and nitrogen supplied MSM giving MSM-CN. 5-TTri removal with the ASC ranged from 60% to 100% in only 10 days. 100 μL suspended biomass from the biodegrading setups was subsequently plated on solid media to eliminate possible activated sludge remnants. After growth occurred, mixed colonies were harvested and inoculated in fresh liquid MSM containing 20 mg L(-1) 5-TTri. These bacterial consortia showed good 5-TTri removal in MSM-CN rather than in MSM, indicating nutrient supply being required for efficient biodegradation. In addition, experiments with high 5-TTri concentrations ranging from 20 to 1,000 mg L(-1) were conducted in both, MSM and MSM-CN and the maximal 5-TTri removal capacity of the ASC evaluated. 50 mg L(-1) 5-TTri was still removed in both media whereas 100 mg L(-1) was solely removed in MSM-CN. 5-TTri biodegradation patterns also indicated that 5-TTri might be co-metabolized by microbial consortia. Furthermore, experiments with gradient-solid-media-plates showed 5-TTri to be inhibitory for the ASC in concentrations above 50 mg L(-1) and revealed the optimal conditions regarding carbon and nitrogen concentration and pH value for effective 5-TTri biodegradation by ASC. Nitrogen proved a crucial factor for enhancing organisms' biodegradation capacity with an optimal pH around 7 while carbon showed no such effect., (Copyright © 2013 Elsevier B.V. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
17. A feedback circuit between transcriptional activation and self-destruction of Gcn4 separates its metabolic and morphogenic response in diploid yeasts.
- Author
-
Herzog B, Streckfuss-Bömeke K, and Braus GH
- Subjects
- Amino Acid Substitution, Basic-Leucine Zipper Transcription Factors chemistry, Cyclins genetics, Dimerization, Diploidy, Feedback, Physiological, Gene Expression Regulation, Developmental, Gene Expression Regulation, Fungal, Genes, Fungal, Lac Operon, Leucine Zippers genetics, Membrane Glycoproteins genetics, Models, Biological, Models, Molecular, Mutant Proteins chemistry, Mutant Proteins genetics, Mutant Proteins metabolism, Protein Stability, Protein Structure, Quaternary, Protein Structure, Tertiary, Saccharomyces cerevisiae growth & development, Saccharomyces cerevisiae Proteins chemistry, Transcriptional Activation, Basic-Leucine Zipper Transcription Factors genetics, Basic-Leucine Zipper Transcription Factors metabolism, Saccharomyces cerevisiae genetics, Saccharomyces cerevisiae metabolism, Saccharomyces cerevisiae Proteins genetics, Saccharomyces cerevisiae Proteins metabolism
- Abstract
The basic zipper Gcn4 protein activates transcription in the yeast Saccharomyces cerevisiae in response to amino acid starvation. This includes numerous metabolic genes of amino acid or purine biosynthesis and the developmental cell-surface flocculin gene FLO11, which is required for diploid pseudohyphae formation and for adhesion upon nutrient starvation. We separated the metabolic from the developmental response by screening for GCN4 alleles that allow growth during amino acid starvation but are impaired in adhesion and are unable to form pseudohyphae. The identified Gcn4(L267S) variant carries an amino acid substitution in the third of the four conserved leucines of the zipper dimerization domain. This mutation abolished FLO11 expression and results in reduced but sufficient transcriptional activity for amino acid biosynthetic genes. The Leu267Ser substitution impairs Gcn4 homodimer formation and is a significantly more stable protein than the wild-type protein. A helix-breaker substitution in Leu253 results in a transcriptionally inactive but highly stable protein variant. This is due to a feedback circuit between transcriptional activity of Gcn4 and its own stability, which depends on the Gcn4-controlled cyclin PCL5. Gcn4(L253G) reduces the expression of Pcl5 and therefore reduces its own degradation. This self-controlled buffer system to restrict transcriptional activity results in a reciprocal correlation between Gcn4 transcriptional activity and protein stability., (Copyright © 2010 Elsevier Ltd. All rights reserved.)
- Published
- 2011
- Full Text
- View/download PDF
18. A sunscreen's labeled sun protection factor may overestimate protection at temperate latitudes: a human in vivo study.
- Author
-
Young AR, Boles J, Herzog B, Osterwalder U, and Baschong W
- Subjects
- Adult, Chemistry, Pharmaceutical, Female, Humans, Male, Models, Theoretical, Radiation Dosage, Skin radiation effects, Skin Neoplasms prevention & control, Spectrum Analysis, Sunburn prevention & control, Treatment Failure, Young Adult, Climate, Drug Labeling standards, Erythema prevention & control, Sunlight adverse effects, Sunscreening Agents therapeutic use, Ultraviolet Rays adverse effects
- Abstract
We lack comparative data on sunscreens with comparable sun protection factors (SPFs), but with different levels of UVA protection, especially against cumulative erythema from repeated suberythemal exposure. Thus, we compared the protection from cumulative sunburn with two sunscreens labeled SPF 6, but with different UVR-absorbing properties, one that absorbs the UVB waveband and the other that absorbs UVB+UVA wavebands. We simulated sunlight typical of temperate latitudes to expose skin daily to suberythemal doses for 13 consecutive days. The study population consisted of eight fair-skinned sun-sensitive healthy young adults. Erythema was assessed by eye and objectively, and the SPF of each sunscreen was modeled with changes in solar UVR with time of day and latitude. The broad-spectrum sunscreen gave much better protection against cumulative erythema than the UVB sunscreen. The explanation for this is that UVA makes a greater contribution toward sunburn at temperate latitudes than under the laboratory conditions in which SPF is tested and assigned. The data support the current trend toward broad-spectrum sunscreen protection. They also show that labeled SPF is much more reliable with broad-spectrum sunscreens because SPF with primarily UVB sunscreens is dependent on time of day and latitude.
- Published
- 2010
- Full Text
- View/download PDF
19. Prediction of sun protection factors and UVA parameters of sunscreens by using a calibrated step film model.
- Author
-
Herzog B, Mongiat S, Quass K, and Deshayes C
- Subjects
- Calibration standards, Predictive Value of Tests, Sunscreening Agents chemistry, Sunscreening Agents standards, Models, Biological, Sunscreening Agents radiation effects, Ultraviolet Rays
- Abstract
At the stage of screening of new sun protection formulations, quick and inexpensive methods for estimation of the ultraviolet (UV) screening performance are highly desirable. It has been shown recently that apart from measurements of sun protection factors in vitro, calculations using a calibrated step film model are also a possibility. In the present investigation, further evidence for the usefulness of the step film model in terms of prediction of the sun protection factor is shown. In addition, it is demonstrated that parameters, which characterize the protection in the UVA range such as the UVA/UVB ratio and the critical wavelength can be calculated in good accordance with experimental data. Although with less accuracy, the estimation of UVA protection factors is also possible, if the photodegradation some filters undergo upon irradiation is taken into consideration., (Copyright 2004 Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 93:1780-1795, 2004)
- Published
- 2004
- Full Text
- View/download PDF
20. The importance of both an early orchidopexy and germ cell maturation for fertility.
- Author
-
Hadziselimovic F and Herzog B
- Subjects
- Humans, Infant, Male, Orchiectomy, Sperm Count, Aging, Cryptorchidism surgery, Fertility
- Abstract
Delayed orchidopexy for undescended testes has been associated with abnormal testicular histology, but the effect on later fertility is unknown. We aimed to establish the importance of the first postnatal maturational step-ie, the transformation of gonocytes into Ad (dark) spermatogonia on fertility. We matched histological findings of the testes from 31 patients who had undergone an early orchidopexy with their total number of sperm. If Ad spermatogonia were present, 17 (94%) of 18 (95% CI 72.7-99.9) of the men had a total sperm count of 40 x 10(6)/ejaculate or greater. By contrast, despite successful early surgery, if Ad spermatogonia were absent, 12 (92%) of 13, (64.0-99.8) patients had abnormal spermiograms. Thus, the transformation of gonocytes into Ad spermatogonia is crucial for male fertility.
- Published
- 2001
- Full Text
- View/download PDF
21. Treatment of cryptorchidism by synthetic luteinising-hormone-releasing hormone.
- Author
-
Hadziselimovic F, Girard J, and Herzog B
- Subjects
- Animals, Child, Child, Preschool, Cryptorchidism blood, Follicle Stimulating Hormone blood, Humans, Luteinizing Hormone blood, Male, Mice, Cryptorchidism drug therapy, Gonadotropin-Releasing Hormone therapeutic use
- Published
- 1977
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.