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The present study aimed to i) assess the disintegration of a novel bio-packaging during aerobic composting (2 and 6 % tested concentrations) and evaluate the resulting compost ii) analyse the ecotoxicity of bioplastics residues on earthworms; iii) study the microbial communities during composting and in 'earthworms' gut after their exposure to bioplastic residues; iv) correlate gut microbiota with ecotoxicity analyses; v) evaluate the chemico-physical characterisation of bio-packaging after composting and earthworms' exposure. Both tested concentrations showed disintegration of bio-packaging close to 90 % from the first sampling time, and compost chemical analyses identified its maturity and stability at the end of the process. Ecotoxicological assessments were then conducted on Eisenia fetida regarding fertility, growth, genotoxic damage, and impacts on the gut microbiome. The bioplastic residues did not influence the earthworms' fertility, but DNA damages were measured at the highest bioplastic dose tested. Furthermore bioplastic residues did not significantly affect the bacterial community during composting, but compost treated with 2 % bio-packaging exhibited greater variability in the fungal communities, including Mortierella, Mucor, and Alternaria genera, which can use bioplastics as a carbon source. Moreover, bioplastic residues influenced gut bacterial communities, with Paenibacillus, Bacillus, Rhizobium, Legionella, and Saccharimonadales genera being particularly abundant at 2 % bioplastic concentration. Higher concentrations affected microbial composition by favouring different genera such as Pseudomonas, Ureibacillus, and Streptococcus. For fungal communities, Pestalotiopsis sp. was found predominantly in earthworms exposed to 2 % bioplastic residues and is potentially linked to its role as a microplastics degrader. After composting, Attenuated Total Reflection analysis on bioplastic residues displayed evidence of ageing with the formation of hydroxyl groups and amidic groups after earthworm exposure., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Composting mitigates environmental risks associated with using solid digestate as fertilizer. However, evidence is lacking on benefits of using composted digestate as fertilizer in enhancing soil health and increasing agronomic yield compared to non-composted digestate (hereafter, digestate). A field study was conducted consisting of digestate, composted digestate, co-composted digestate with biogas feedstocks (corn [Zea mays L.] silage, poultry litter, corn silage + poultry litter or food processing by-product), inorganic nitrogen fertilizer, and control (no treatment applied) on soil microbial biomass, enzyme activities (EA), soil organic carbon (SOC), bioavailable P (P), total nitrogen (TN), soil health index (SHI), and sunflower (Helianthus annuus L.) yield. The Partial Least Square Path Model (PLS-PM) was used to predict: 1) nutrient cycling in response to changes in microbial growth and EA and 2) agronomic yield in response to SHI and soil nutrients dynamics. Composted digestate had equivalent soil health benefits with most of co-composted materials and digestate, albeit agronomic yield was greatest with composted digestate, which was 40 % and 100 % greater than with inorganic nitrogen fertilizer and digestate, respectively, indicating composted digestate's potential to replace the synthetic N fertilizer. Moreover, composts from a sole digestate, rather than the ones from co-composted with fresh feedsstocks, can be promising organic amendments and fertilizers for growing sunflower. The PLS-PM model identified that triggered microbial biomass growth and EA, following digestate and composted digestate applications, catalyzed organic matter decomposition, resulting in enhanced nutrients contents and soil health. However, the model revealed that improved SHI did not predict agronomic yield, as opposed to P and TN, suggesting agronomic performance may have been more sensitive to changes in specific soil nutrients status than the overall soil health condition. We conclude that the benefits of composted digestate as fertilizer hint the significance of digestate valorization via post-digestate composting and compost utilization for sustainability of the bioenergy sector., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)