Author: "James C Lee" / Publisher: elsevier - Searchworks@Jio Institute Digital Library Search Results

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Start Over Author "James C Lee" Publisher elsevier

Author: Jae-Won Shin and James C. Lee
Subjects: 0301 basic medicine, Mice, Transgenic, Endocytosis, Article, Exocytosis, Mice, 03 medical and health sciences, 0302 clinical medicine, Alzheimer Disease, Caveolae, medicine, Animals, Lipid raft, Lipid Transport, Amyloid beta-Peptides, Microglia, Chemistry, Lipid metabolism, Microvesicles, Cell biology, Disease Models, Animal, 030104 developmental biology, medicine.anatomical_structure, lipids (amino acids, peptides, and proteins), 030217 neurology & neurosurgery
Abstract: The majority of Alzheimer's disease (AD) risk genes are highly and selectively expressed by microglia in the brain. Several of these genes are related to lipid and cholesterol metabolism, lipid synthesis, lipid transport, endocytosis, exocytosis and phagocytosis. Therefore, studying the roles of cellular membrane biophysics in microglial function should improve our understanding of the AD pathology. In this chapter, we discuss how lipid rafts and membrane-cytoskeleton adhesion impact microglial-mediated oxidative stress and clearance of amyloid-β peptide (Aβ). We also discuss potential roles of lipid membrane-bound extracellular vesicles as carriers of pathological factors to promote inflammation and cytotoxicity.
Published: 2020

Author: James C. Lee
Subjects: Tubulin, Microtubule, Homogeneous, Protein subunit, Organelle, biology.protein, Biology, Plant cell, Peptide sequence, In vitro, Cell biology
Abstract: Publisher Summary This chapter presents the modified procedure involved in purifying brain tubulin developed originally by Weisenberg and presents the current information on the structure of tubulin. Microtubules are found in a wide variety of animal and plant cells. In dividing cells they are localized in the mitotic apparatus and are also found in axonal and dendritic processions of neurons. They have been implicated in a number of different cellular functions. Structurally, microtubules are composed of protofilaments of the subunit protein, tubulin. Because of the wide distribution of this organelle and the large number of different cellular processes in which it seems to participate, microtubule has been the target of intensive investigation. The purification procedure enables obtaining tubulin greater than 95% homogeneous. By employing highly purified samples, it is much easier to establish a meaningful structure–function relationship with minimal complication from other components. Employing such protein helps establish the minimum conditions in which the protein can undergo in vitro reconstitution and ligand-induced polymerization.
Published: 1982

Author: Alejandro Lemor, MD, MS, Mir B. Basir, DO, Brian P. O'Neill, MD, Jennifer Cowger, MD, Tiberio Frisoli, MD, James C. Lee, MD, Dee Dee Wang, MD, Khaldoon Alaswad, MD, William O’Neill, MD, and Pedro A. Villablanca, MD, MS
Subjects: Diseases of the circulatory (Cardiovascular) system, RC666-701
Abstract: Veno-arterial extracorporeal membrane oxygenation is used in patients requiring biventricular support; however, its use increases the afterload. In patients with severe aortic insufficiency or severe left ventricular disfunction, it will increase left-side filling pressures, hence the need for left ventricle unloading with an additional mechanical circulatory support device. We present a case of a patient with cardiogenic shock and severe aortic insufficiency who underwent left atrial veno-arterial extracorporeal membrane oxygenation and provide a step-by-step explanation of the technique.
Published: 2022
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Author: Tiberio Michele Frisoli, MD, Chak-yu So, MBChB, Jayakar G. Guruswamy, MD, Alex Bou Chebl, MD, James C. Lee, MD, and Marvin H. Eng, MD
Subjects: AngioVac, ascending aortic thrombus, aspiration thrombectomy, cerebral embolic protection, Diseases of the circulatory (Cardiovascular) system, RC666-701
Abstract: A patient had a stroke caused by a large, pedunculated aortic ascending mass and was deemed at high risk for near-term recurrent stroke. This case illustrates percutaneous aspiration thrombectomy of ascending aortic thrombus with the AngioVac system (Angiodynamics, Latham, New York), with conscious sedation for early stroke detection and with endovascular cerebral embolic protection. (Level of Difficulty: Intermediate.)
Published: 2020
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Author: Abigail F. Groff, Diana B. Sanchez-Gomez, Marcela M.L. Soruco, Chiara Gerhardinger, A. Rasim Barutcu, Eric Li, Lara Elcavage, Olivia Plana, Lluvia V. Sanchez, James C. Lee, Martin Sauvageau, and John L. Rinn
Subjects: Biology (General), QH301-705.5
Abstract: The Linc-p21 locus, encoding a long non-coding RNA, plays an important role in p53 signaling, cell-cycle regulation, and tumor suppression. However, despite extensive study, confusion exists regarding its mechanism of action: is activity driven by the transcript acting in trans, in cis, or by an underlying functional enhancer? Here, using a knockout mouse model and a massively parallel enhancer assay, we delineate the functional elements at this locus. We observe that, even in tissues with no detectable Linc-p21 transcript, deletion of the locus significantly affects local gene expression, including of the cell-cycle regulator Cdkn1a. To characterize this RNA-independent regulatory effect, we systematically interrogated the underlying DNA sequence for enhancer activity at nucleotide resolution and confirmed the existence of multiple enhancer elements. Together, these data suggest that, in vivo, the cis-regulatory effects mediated by Linc-p21, in the presence or absence of transcription, are due to DNA enhancer elements.
Published: 2016
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