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11 results on '"James V. Jester"'

1. Origin and Lineage Plasticity of Endogenous Lacrimal Gland Epithelial Stem/Progenitor Cells

Author

Liana Basova, Geraint J. Parfitt, Alex Richardson, Vanessa Delcroix, Takeshi Umazume, Daniel Pelaez, David T. Tse, Ivo Kalajzic, Nick Di Girolamo, James V. Jester, and Helen P. Makarenkova

Subjects
Biological Sciences, Cell Biology, Stem Cells Research, Developmental Biology, Science
Abstract

Summary: The lacrimal gland (LG) is an exocrine organ responsible for the secretion of aqueous tear film. Regenerative and stem cell therapies that target LG repair are coming to the fore, although our understanding of LG cell lineage hierarchy is still incomplete. We utilize the analysis of label-retaining cells (LRCs) and genetic lineage tracing to define LG cell lineage hierarchy. Our study suggests that embryonic LG contains unique long-lived multipotent stem cells that give rise to all postnatal epithelial cell types. Following birth, lineages become established and the fate of progenitor cell descendants becomes restricted. However, some cell lineages retain plasticity after maturation and can trans-differentiate into other cell types upon injury. The demonstration that the LG contains progenitor cells with different levels of plasticity has profound implications for our understanding of LG gland function in homeostasis and disease and will be helpful for developing stem cell-based therapies in the future.

Published
2020
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2. Renewal of the Holocrine Meibomian Glands by Label-Retaining, Unipotent Epithelial Progenitors

Author

Geraint J. Parfitt, Phillip N. Lewis, Robert D. Young, Alex Richardson, J. Guy Lyons, Nick Di Girolamo, and James V. Jester

Subjects
3D reconstruction, meibomian gland, adult stem cells, H2B-GFP/K5tTA, Medicine (General), R5-920, Biology (General), QH301-705.5
Abstract

The meibomian and sebaceous glands secrete lipids to prevent desiccation of the ocular surface and skin, respectively. Precisely how these holocrine tissues regenerate is not well understood. To address this, we characterized keratin 5+ (K5) label-retaining cells (LRCs) and the lineage tracing of keratin 14 (K14) progenitors in mouse meibomian glands. Using the tet-off H2B-GFP/K5tTA mouse, H2B-GFP fluorescence dilutes 2-fold with every division in K5+ cell nuclei after doxycycline administration. In 3D reconstructions generated over a >28-day doxycycline chase, we observed LRCs at the acinus entrance where K6+ ductal epithelium terminates. For lineage tracing, K14CreERT2-Confetti mice were injected intraperitoneally with tamoxifen and euthanized at 23 and 59 weeks later. Meibomian gland acini in these mice were either monochromatic or dual-colored, whereas the duct exhibited multiple colors. In conclusion, LRCs are likely to direct meibomian gland turnover and may exist as two distinct unipotent progenitors that renew ductal and acinar tissue separately.

Published
2016
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3. List of Contributors

Author

Elena Albé, Jorge L Alio, Norma Allemann, Jean-Louis Arné, M Farooq Ashraf, Kerry Assil, Nathalie F Azar, Dimitri T Azar, Georges Baikoff, Richard E Braunstein, Salim I. Butrus, Jonathan Carr, Cesar E Carriazo, Fábio Henrique Casanova, Wallace Chamon, Tat Keong Chan, Daniel H Chang, Philippe Chastang, Pauline Cho, Elizabeth A Davis, Jose de la Cruz, Samir G Farah, Damien Gatinel, Ramon C Ghanem, Rola N Hamam, David R Hardten, Peter S Hersh, Arthur Ho, Thanh Hoang-Xuan, Brien A Holden, Sandeep Jain, Elias F Jarade, Joel Adrien D Javier, James V Jester, Maria I Kalyvianaki, Vikentia J Katsanevaki, Johnny M Khoury, Lynda Kleiman, Michael C Knorz, Jeffrey C Lamkin, François Malecaze, Fabrice Manns, Marguerite B McDonald, John Mountford, Francoise Abi Nader, Irini I Naoumidi, Lee T Nordan, Ioannis G Palliakaris, Jean-Marie Parel, Juan J Perez-Santonja, Antony M Poothullil, Wilfried J Rademaker, Ali Al Rajhi, Cynthia Roberts, Mark Rosenblatt, Mirwat Sami, David J Schanzlin, Theo Seiler, Ashish G Sharma, Janine Smith, Walter Stark, Kalliopi Stasi, Leon Strauss, Samar Al Swailem, Suphi Taneri, Vance Thompson, Josep Torras, Kazuo Tsubota, Frédéric Vayr, Steven M Verity, Michael D Wagoner, Jayne S Weiss, Albert CM Wong, and Richard W Yee

Published
2021
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4. Cell regulation of collagen fibril macrostructure during corneal morphogenesis

Author

Eric Mikula, Andrew J. Quantock, James V. Jester, Robert D. Young, Elena Koudouna, and Donald J. Brown

Subjects
0301 basic medicine, Corneal development, Stromal cell, 1.1 Normal biological development and functioning, Corneal Stroma, Fibrillar Collagens, Morphogenesis, Biomedical Engineering, Bioengineering, Chick Embryo, Eye, Biochemistry, Second harmonic generation imaging, Article, Collagen fibril, Biomaterials, Cornea, 03 medical and health sciences, Stroma, Form and function, Underpinning research, medicine, 2.1 Biological and endogenous factors, Animals, Aetiology, Molecular Biology, Eye Disease and Disorders of Vision, Intraocular Pressure, Chemistry, Cell regulation, General Medicine, Embryonic stem cell, eye diseases, Cell biology, Biomechanical Phenomena, 030104 developmental biology, medicine.anatomical_structure, Cell-matrix interactions, Collagen, sense organs, Keratocytes, Biotechnology
Abstract

While tissue form and function is highly dependent upon tissue-specific collagen composition and organization, little is known of the mechanisms controlling the bundling of collagen fibrils into fibers and larger structural designs that lead to the formation of bones, tendons and other tissues. Using the cornea as a model system, our previous 3 dimensional mapping of collagen fiber organization has demonstrated that macrostructural organization of collagen fibers involving interweaving, branching and anastomosing plays a critical role in controlling mechanical stiffness, corneal shape and refractive power. In this work, the cellular and mechanical mechanisms regulating critical events in the assembly of collagen macrostructure are analysed in the developing chicken cornea. We elucidated the temporal events leading to adult corneal structure and determined the effects of intraocular pressure (IOP) on the organization of the collagen macrostructure. Our findings indicate that the complex adult collagen organization begins to appear on embryonic day 10 (E10) after deposition of the primary stroma and full invasion of keratocytes. Importantly, organizational changes in keratocytes appearing at E9 preceded and predicted later changes in collagen organization. Corneal collagen organization remained unaffected when the development of IOP was blocked at E4. These findings support a primary role for keratocytes in controlling stromal organization, mechanical stiffness and corneal shape that are not regulated by the IOP. Our findings also suggest that the avian cornea represents an excellent experimental model for elucidating key regulatory steps and mechanisms controlling the collagen fiber organization that is critical to determining tissue form and function.Statement of significanceThis work by using an ex ovo model system, begins to investigate the potential mechanisms controlling collagen fibril macrostructure. In particular, this work highlights a convergent role for the corneal keratocytes in organizing the complex collagen macrostructure, necessary to support high visual acuity. Our data supports that the intraocular pressure does not influence collagen fibril macrostructure and suggest that the avian cornea represents an excellent experimental model for elucidating key regulatory steps and mechanisms controlling the collagen fiber organization that is critical to determining tissue form and function. Clearly understanding the cellular and molecular mechanisms that underlie collagen fibril macrostructure will be highly beneficial for future tissue engineering and regenerative medicine applications.

Published
2018

5. Meibocyte differentiation and renewal: Insights into novel mechanisms of meibomian gland dysfunction (MGD)

Author

Geraint J. Parfitt, James V. Jester, Ho Sik Hwang, and Donald J. Brown

Subjects
0301 basic medicine, Aging, PPARγ, Ocular surface, Meibomian gland, Medical Biochemistry and Metabolomics, Ophthalmology & Optometry, 0302 clinical medicine, Theoretical, Models, 2.1 Biological and endogenous factors, Cell Self Renewal, Aetiology, Receptor, Stem cell, Meibomian Glands, Cell Differentiation, Peroxisome, Lipids, Sensory Systems, Cell biology, medicine.anatomical_structure, Dry Eye Syndromes, Signal Transduction, medicine.medical_specialty, 1.1 Normal biological development and functioning, Dry eye, Biology, Article, 03 medical and health sciences, Cellular and Molecular Neuroscience, Atrophy, Underpinning research, Opthalmology and Optometry, Internal medicine, medicine, Eye Disease and Disorders of Vision, Inflammatory and immune system, Neurosciences, Lipid metabolism, Models, Theoretical, Stem Cell Research, medicine.disease, Meibocyte, PPAR gamma, Ophthalmology, 030104 developmental biology, Endocrinology, Nuclear receptor, 030221 ophthalmology & optometry, Function (biology)
Abstract

This paper reviews our current understanding of age-related meibomian gland dysfunction (MGD) and the role of the nuclear receptor, peroxisome proliferator-activated receptor gamma (PPARγ), in the regulation of meibomian gland function, meibocyte differentiation and lipid synthesis. The studies suggest that PPARγ is a master regulator of meibocyte differentiation and function, whose expression and nuclear signaling coupled with meibocyte renewal is altered during aging, potentially leading to atrophy of the meibomian gland as seen in clinical MGD. Study of meibomian gland stem cells also suggest that there is a limited number of precursor meibocytes that provide progeny to the acini, that may be susceptible to exhaustion as occurs during aging and other environmental factors. Further study of pathways regulating PPARγ expression and function as well as meibocyte stem cell maintenance may provide clues to establishing cellular and molecular mechanisms underlying MGD and the development of novel therapeutic strategies to treating this disease.

Published
2017

6. A novel, long-lived, and highly engraftable immunodeficient mouse model of mucopolysaccharidosis type I

Author

Daniel C. Mendez, Philip H. Schwartz, Patricia I. Dickson, Alexander E. Stover, Cian McGuire, Andrew Wong, James V. Jester, Anthony D. Rangel, Marissa A. Torres, Hubert E. Nethercott, Steven Q. Le, Jonathan D. Cooper, Shih hsin Kan, Edwin S. Monuki, David J. Brick, and Omar Khalid

Subjects
lcsh:QH426-470, Nod, Article, 03 medical and health sciences, Mucopolysaccharidosis type I, 0302 clinical medicine, Genetics, medicine, lcsh:QH573-671, Induced pluripotent stem cell, Molecular Biology, 030304 developmental biology, 0303 health sciences, business.industry, lcsh:Cytology, Organ dysfunction, Transplantation, Haematopoiesis, lcsh:Genetics, Gliosis, Immunology, Molecular Medicine, medicine.symptom, Stem cell, business, 030217 neurology & neurosurgery
Abstract

Mucopolysaccharidosis type I (MPS I) is an inherited α-L-iduronidase (IDUA, I) deficiency in which glycosaminoglycan (GAG) accumulation causes progressive multisystem organ dysfunction, neurological impairment, and death. Current MPS I mouse models, based on a NOD/SCID (NS) background, are short-lived, providing a very narrow window to assess the long-term efficacy of therapeutic interventions. They also develop thymic lymphomas, making the assessment of potential tumorigenicity of human stem cell transplantation problematic. We therefore developed a new MPS I model based on a NOD/SCID/Il2rγ (NSG) background. This model lives longer than 1 year and is tumor-free during that time. NSG MPS I (NSGI) mice exhibit the typical phenotypic features of MPS I including coarsened fur and facial features, reduced/abnormal gait, kyphosis, and corneal clouding. IDUA is undetectable in all tissues examined while GAG levels are dramatically higher in most tissues. NSGI brain shows a significant inflammatory response and prominent gliosis. Neurological MPS I manifestations are evidenced by impaired performance in behavioral tests. Human neural and hematopoietic stem cells were found to readily engraft, with human cells detectable for at least 1 year posttransplantation. This new MPS I model is thus suitable for preclinical testing of novel pluripotent stem cell-based therapy approaches.

Published
2015
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7. Contributors

Author

Richard L Abbott, Sean D Adrean, Abdulrahman Al-Muammar, Jihan Akhtar, Eduardo C Alfonso, Richard C Allen, M Camille Almond, Lênio Alvarenga, Wallace LM Alward, Renato Ambrósio, Mohammad Anwar, Dimitri T Azar, James L Ball, Neal P Barney, Rebecca M Bartow, Jules Baum, Michael W Belin, Jason H Bell, Beth Ann Benetz, Zachary Berbos, Roger W Beuerman, Arpita Kadakia Bhasin, Pooja V Bhat, Joseph M Biber, Maria Bidros, Andrea D Birnbaum, Charles S Bouchard, Jay C Bradley, James D Brandt, Richard D Brasington, Harilaos S Brilakis, Cat N Burkat, Marta Calatayud, J Douglas Cameron, Mauro Campos, Emmett F Carpel, H Dwight Cavanagh, Cordelia Chan, Richard I Chang, Bernard H Chang, Kenneth C Chern, Steven Ching, James Chodosh, Phillip H Choo, Gary Chung, Joseph B Ciolino, Janine A Clayton, Elisabeth J Cohen, Oliver Comyn, M Soledad Cortina, John W Cowden, Christopher R Croasdale, Richard S Davidson, Elizabeth A Davis, Sheraz M Daya, Denise de Freitas, David L DeMill, Lauro Augusto de Oliveira, Marc D de Smet, Luciene B de Sousa, Ali R Djalilian, Claes H Dohlman, Eric D Donnenfeld, Richard K Dortzbach, William T Driebe, Steven P Dunn, Ralph C Eagle, Sean L Edelstein, Richard A Eiferman, Joseph A Eliason, Marjan Farid, William J Faulkner, Robert S Feder, Vahid Feiz, Matthew T Feng, John H Fingert, George J Florakis, Luigi Fontana, Richard K Forster, C Stephen Foster, F Stuart Foster, Gary N Foulks, Mitchell H Friedlander, Masahiko Fukuda, Anat Galor, Theresa J Gan, Prashant Garg, Sumit Garg, David B Glasser, Kenneth M Goins, Debra A Goldstein, Chloe Gottlieb, Michael R Grimmett, Oscar Gris, Erich B Groos, William D Gruzensky, Jose L Güell, Preeya K Gupta, M Bowes Hamill, Kristin M Hammersmith, Pedram Hamrah, Sadeer B Hannush, David R Hardten, Andrew Harrison, Ellen L Heck, David G Heidemann, David C Herman, J Martin Heur, William G Hodge, Carol J Hoffman, Edward J Holland, Gary N Holland, Marc A Honig, Christopher T Hood, Eliza N Hoskins, Andrew J W Huang, David Huang, Jennifer I Hui, Joseph D Iuorno, W Bruce Jackson, Frederick A Jakobiec, Bennie H Jeng, James V Jester, David R Jordan, Terry L Kaiura, Carol L Karp, Douglas G Katz, Stephen C Kaufman, Robert C Kersten, Stephen S Khachikian, Jennifer H Kim, Joung Y Kim, Stella K Kim, Terry Kim, Colin M Kirkness, Stephen D Klyce, Douglas D Koch, Regis P Kowalski, Jay H Krachmer, Peter R Laibson, Stephen S Lane, Jonathan H Lass, W Barry Lee, Olivia A Lee, Michael A Lemp, Phoebe D Lenhart, Yan Li, Thomas J Liesegang, Michele C Lim, Lily Koo Lin, Michael P Lin, Thomas D Lindquist, Richard L Lindstrom, David Litoff, Christopher Liu, Careen Y Lowder, Anthony J Lubniewski, Hall T McGee, Ian W McLean, Marian S Macsai, Felicidad Manero, Mark J Mannis, Dimosthenis Mantopoulos, Carlos E Martinez, Csaba L Mártonyi, Raneen S Mashor, William D Mathers, Manisha N Mehta, David M Meisler, Shahzad I Mian, Darlene Miller, Corey A Miller, Monty Montoya, Merce Morral, Andrew L Moyes, Michael L Murphy, Nariman Nassiri, Kristiana D Neff, J Daniel Nelson, Jeffrey A Nerad, Marcelo V Netto, Christopher J Newton, Lisa M Nijm, Teruo Nishida, Bruce A Noble, Michael L Nordlund, Robert B Nussenblatt, David G O'Day, Jenny V Ongkosuwito, Karen W Oxford, David A Palay, Florentino E Palmon, Deval R Paranjpe, Mansi Parikh, David H Park, D J John Park, Matthew R Parsons, Charles J Pavlin, Eric S Pearlstein, Alicia Perry, W Matthew Petroll, Daryl R Pfister, Roswell R Pfister, Stephen C Pflugfelder, Francis W Price, Marianne O Price, Louis E Probst, John J Purcell, Andrew A E Pyott, Michael B Raizman, Leela V Raju, J Bradley Randleman, Gullapalli N Rao, Christopher J Rapuano, Charles D Reilly, Adimara de Candelaria Renesto, Renata A Rezende, Danielle M Robertson, David S Rootman, Jason S Rothman, Roy Scott Rubinfeld, Alan E Sadowsky, Shizuya Saika, Monali V Sakhalkar, James J Salz, Virender S Sangwan, Marinho Scarpi, Bradley H Scharf, Greg Schmidt, Artur Schmitt, Fernanda Piccoli Schmitt, Miriam T Schteingart, Ivan R Schwab, Brian L Schwam, Gary S Schwartz, H Nida Sen, Michael B Shapiro, Shigeto Shimmura, Neera Singal, Heather M Skeens, Craig A Skolnick, Allan R Slomovic, Janine A Smith, Michael E Snyder, Renée Solomon, Sarkis H Soukiasian, Sathish Srinivasan, John F Stamler, Roger F Steinert, Glenn L Stoller, Barbara W Streeten, R Doyle Stulting, Alan Sugar, Joel Sugar, Donald Tan, Joseph Tauber, Mark A Terry, Howard H Tessler, Marta Torrabadella, Elias I Traboulsi, William B Trattler, Julie H Tsai, David T Tse, Elmer Y Tu, Roxana Ursea, Pravin K Vaddavalli, Woodford S Van Meter, Gary A Varley, Roshni Vasaiwala, Anthony J Verachtert, David D Verdier, Ana Carolina Vieira, Vanee V Virasch, Li Wang, George O Waring, Michael A Warner, Kevin J Warrian, Guy F Webster, Mitchell P Weikert, Robert W Weisenthal, Jayne S Weiss, Pongmas Wichiensin, Kirk R Wilhelmus, Steven E Wilson, Maria A Woodward, Richard W Yee, and Sonya Yoo

Published
2011
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11. [14] Measurement of tissue thickness using confocal microscopy

Author

James V. Jester, Walter M Petroll, and Harrison D Cavanagh

Subjects
Materials science, Microscope, law, Confocal microscopy, Confocal, Tissue thickness, Scanning confocal electron microscopy, Nanotechnology, Pinhole, Nipkow disk, Numerical aperture, Biomedical engineering, law.invention
Abstract

Publisher Summary The chapter presents a discussion on measurement of tissue thickness using confocal microscopy. The major technical details concerning the in vivo measurement of tissue thickness using reflected light confocal microscopy are discussed. Critical to the in vivo measurement of tissue thickness using confocal microscopy is the imaging of tissue in real time. This is necessary to avoid unwanted irregular movement of the tissue because of pulse and respiration and to capture fast temporal events, such as blood flow or acute cellular responses, which can blur images at high magnification. There are several advantages to the tandem scanning confocal microscope (TSCM) for use in evaluating living tissue, first of which is the real time imaging capabilities. The white light used is also not toxic to tissues and produces minimal discomfort. Additionally, the use of diffraction-limited pinholes provides optimal lateral and axial resolution when using objectives of the same numerical aperture. As an alternative to the tandem scanning design, a one-sided Nipkow disk system where illumination and detection occur simultaneously through the same pinhole is developed. The chapter also discusses the measurement of tissue thickness by using a depth encoding system (DES) and by rapid through focusing approach.

Published
1999
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