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Hazardous heavy metals and organic substances removal is of great significance for ensuring the safety of aquatic-ecosystem, yet the highly effective and selective extraction always remains challenging. To address this problem, magnetic hollow microcubes were fabricated through thermal carbonization of Fe 3 O 4 -COOH@ γ-CD-MOFs, and core-shell structured precursors were in-situ greenly constructed on a large scale via microwave-assisted self-assembly strategy. As noted, the development of secondary crystallization was utilized to achieve uniform dispersion of cores within MOFs frameworks and thus improved magnetic and adsorption ability of composites. Acquired magnetic Fe 3 O 4 @HC not only can harvest excellent extraction of heavy metals (Cd, Pb, and Cu of 129.87, 151.05, and 106.98 mg·g -1 ) but also exhibit highly selective adsorption ability for cationic organics (separation efficiency higher than 95.0 %). Impressively, Fe 3 O 4 @HC achieved outstanding adsorption (60-80 %) of Cd in realistic mussel cooking broth with no obvious loss in amino acid. Characterizations better offer mechanistic insight into the enhanced selectivity of positively charged pollutants can be attributed to synergistic effect of ions exchange and electrostatic interaction of abundant oxygen-containing functional groups. Our study provides a feasible route by rationally developing core-shell structured composites to promote the practical applications of sustainable water treatment and value-added utilization of processing by-products., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The presence of pesticide residues in aquatic environments poses a significant threat to both aquatic ecosystems and human health. The presence of these residues can result in significant harm to aquatic ecosystems and can negatively impact the health of aquatic organisms. Consequently, this issue requires urgent attention and effective measures to mitigate its impact. However, developing sensitive and rapid detection methods remains a challenge. In this study, an all-in-one test strip, which integrated bioenzymes, nanoenzymes, and a chromogen, was developed in combination with an enzyme labeling instrument for a highly sensitive and convenient sensing of malathion residues. The oxidase activity of heme chloride (Hemin) in the strip can catalyze the oxidation of H 2 O 2 and 3,3',5,5'-tetramethylbenzidine (TMB) to produce a blue-colored oxide. Simultaneously, the alkaline phosphatase (ALP) present in the strip can break down l-ascorbic acid-2-phosphate to produce ascorbic acid (AA). This AA then acts to reduce the oxidized form of TMB, turning it into a colorless substance and leading to the disappearance of its fluorescent signal. In the presence of a pesticide, the activity of ALP is inhibited and formation of AA is blocked, thereby preventing the reduction of oxidized TMB and producing a colored signal. According to this principle, the integrated test strip detected the target pesticide with high performance as per the optical density value determined via an enzyme marker. The detection limit of the test strip was 0.209 ng/mL with good sensitivity. The method was used for detecting malathion in actual river water samples, and the recoveries were in the range of 93.53 %-96.87 %. The newly devised technique effectively identified malathion in samples of natural water. This research has introduced a novel approach for the precise and convenient surveillance of pesticide remnants. Additionally, these discoveries could inspire the advancement of proficient multi-enzyme detection systems., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

CCL17 is well known for its ability to engage CCR4 + CD8 + T cells, which have been shown to play a critical role in preventing tumorigenesis. In this study, we attempted to inhibit in vivo pancreatic tumorigenesis by engaging murine CCR4 + CD8 + T cells through a drug-eluting scaffold with a payload of CCL17. The drug-eluting scaffold was fabricated by electrospinning polyglyconate and porcine gelatin. The electrospun scaffold featured randomly distributed non-woven fibers with diameters ranging from 1μm to 4μm. The in vitro study confirmed that scaffolding materials were non-cytotoxic to pancreatic cancer cells. The in vivo study showed an increased presence of murine CCR4 + CD8 + T cells into the tumor mass treated with drug-eluting scaffold compared to those with non-eluting scaffold or the control groups. The weights of tumor masses were 132.04mg±12.25mg in the control group, 158.12mg±18.98mg in the NES group and 96.22mg±14.56mg in the DES group, respectively. The volumes of tumor masses were 1035.21mm 3 ±128.97mm 3 in the control group, 978.56mm 3 ±110.19mm 3 in the NES group and 634.35mm 3 ±87.12mm 3 in the DES group, respectively. Further study showed that the increased presence of CCR4 + CD8 + T cells also inhibited the hepatic metastasis of pancreatic cancer cells. Our study shed a new light on the post-operative treatment of pancreatic cancer to prevent the recurrence., (Copyright © 2017 Elsevier B.V. All rights reserved.)

A variety of personal care products have been classified as emerging contaminants (ECs). Occurrence, fate, spatial and vertical profiles of 13 ultraviolet absorbents, triclocarban (TCC) and its dechlorinated products, triclosan (TCS), 2-phenylphenol and parabens were investigated in riverine and estuarine sediment of the Pearl River catchment, China. Bisphenol A (BPA), a widely applied plasticizer, was also investigated. The ECs were widely present in the bed sediment. TCC was the most abundant with a maximum concentration of 332ngg -1 dry weight. The other prominent ECs included BPA, TCS, octocrylene, and benzotriazole UV stabilizers UV326 and UV328. Treated wastewater effluent was the major source of the ECs in the riverine sediment. TCC, BPA, TCS, methyparaben, UV531, UV326, and UV328 were also detected throughout the estuarine sediment cores, indicating their persistence in the sediment. Temporal trends of the ECs in the sediment cores reflected a combined effect of industrial development, population growth, human life quality improvement, and waste treatment capacity in the Pearl River Delta over the last decades. TCC dechlorination products were frequently detected in the bed sediment with higher levels near treated effluent outlets but only occasionally observed in the sediment cores, suggesting insignificant in-situ TCC dechlorination in the sediment., (Copyright © 2016 Elsevier B.V. All rights reserved.)

Metabolite identification is crucial for revealing metabolic pathways and comprehensive potential toxicities of polycyclic aromatic hydrocarbons (PAHs) in human body. In this work, a quasi-targeted analysis strategy was proposed for metabolite identification of monohydroxylated polycyclic aromatic hydrocarbons (OH-PAHs) in human urine using liquid chromatography triple quadruple mass spectrometry (LC-QqQ-MS/MS) combined with liquid chromatography high resolution mass spectrometry (LC-HRMS). Potential metabolites of OH-PAHs were preliminarily screened out by LC-QqQ-MS/MS in association with filtering in a self-constructed information list of possible metabolites, followed by further identification and confirmation with LC-HRMS. The developed method can provide more reliable and systematic results compared with traditional untargeted analysis using LC-HRMS. In addition, data processing for LC-HRMS analysis were greatly simplified. This quasi-targeted analysis method was successfully applied to identifying phase I and phase II metabolites of OH-PAHs in human urine. Five metabolites of hydroxynaphthalene, seven of hydroxyfluorene, four of hydroxyphenanthrene, and three of hydroxypyrene were tentatively identified. Metabolic pathways of PAHs in human body were putatively revealed based on the identified metabolites. The experimental results will be valuable for investigating the metabolic processes of PAHs in human body, and the quasi-targeted analysis strategy can be expanded to the metabolite identification and profiling of other compounds in vivo., (Copyright © 2016 Elsevier B.V. All rights reserved.)

A sensitive and reliable method was developed for multi-target determination of 13 most widely used organic ultraviolet (UV) absorbents (including UV filters and UV stabilizers) in aquatic organism tissues. The organic UV absorbents were extracted using ultrasonic-assisted extraction, purified via gel permeation chromatography coupled with silica gel column chromatography, and determined by ultra-high performance liquid chromatography-tandem mass spectrometry. Recoveries of the UV absorbents from organism tissues mostly ranged from 70% to 120% from fish filet with satisfactory reproducibility. Method quantification limits were 0.003-1.0ngg(-1) dry weight (dw) except for 2-ethylhexyl 4-methoxycinnamate. This method has been applied to analysis of the UV absorbents in wild and farmed aquatic organisms collected from the Pearl River Estuary, South China. 2-Hydroxy-4-methoxybenzophenone and UV-P were frequently detected in both wild and farmed marine organisms at low ngg(-1)dw. 3-(4-Methylbenzylidene)camphor and most of the benzotriazole UV stabilizers were also frequently detected in maricultured fish. Octocrylene and 2-ethylhexyl 4-methoxycinnamate were not detected in any sample. This work lays basis for in-depth study about bioaccumulation and biomagnification of the UV absorbents in marine environment., (Copyright © 2015 Elsevier B.V. All rights reserved.)

Pharmaceutical and personal care products (PPCPs), including antibiotics, azole anti-fungals, non-steroid anti-inflammatory drugs, lipid regulators, parabens, antiseptics, and bisphenol A, were investigated in groundwater and reservoirs in the vicinity of two municipal landfills in the metropolis of Guangzhou, South China. Dehydroerythromycin, sulfamethoxazole, fluconazole, salicylic acid, methylparaben, triclosan, and bisphenol A were the mostly frequently detected PPCPs in the groundwater at low ng L(-1) levels. In the reservoirs, the PPCPs were widely detected at higher frequencies and concentrations, especially sulfamethoxazole, propiconazole, and ibuprofen, with maximal concentrations above 1 μg L(-1). The PPCPs in the groundwater did not show significant seasonal differences or spatial trends. However, in the reservoirs, higher PPCP concentrations were observed in spring than in other seasons. The anti-bacterials in the groundwater posed medium risks to algae. In the reservoirs, the sulfonamides and macrolides posed low to high risks, while ibuprofen, salicylic acid, and clofibric acid presented low to medium risks to aquatic organisms. Overall, the results showed that the PPCP contaminants and subsequent ecological risks in the groundwater and surface water in the vicinity of the landfills may be of serious concern. More research is needed to better correlate the landfill leachates and PPCP contamination in the nearby aquatic environments., (Copyright © 2014 Elsevier B.V. All rights reserved.)