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Your search keyword '"Jonathan M. Wastling"' showing total 4 results
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4 results on '"Jonathan M. Wastling"'

1. Proteomics and posttranslational protein modifications in Toxoplasma gondii

Author

William J. Sullivan, Victoria Jeffers, Jonathan M. Wastling, Louis M. Weiss, and Kami Kim

Subjects
Research groups, biology, parasitic diseases, Toxoplasma gondii, Identification (biology), Genome project, Computational biology, Proteomics, biology.organism_classification, Gene, Function (biology), Organism
Abstract

The identification and characterization of the function of proteins in Toxoplasma gondii has been a focus of many research groups since the development of techniques that enabled the study of individual genes and molecules. Proteomic studies provide complementary data to those obtained by transcriptional “omics” approaches. Proteomics data for T. gondii has also been helpful for improving genome annotation. Improved methods to purify subproteomes and the use of biotin identification (and similar techniques) are dramatically expanding our understanding of the structures formed by this organism and their relationship to its host cell. This chapter reviews the current status of proteomic studies on T. gondii and the role of various posttranslational modifications in the biology of this pathogen.

Published
2020

2. List of Contributors

Author

James W. Ajioka, Daniel Ajzenberg, James Alexander, Takashi Asai, Matthew Bogyo, John C. Boothroyd, Barbara A. Butcher, David J. Bzik, Vern B. Carruthers, Marie-France Cesbron-Delauw, Kshitiz Chaudhary, Isabelle Coppens, Marie-Laure Dardé, Eric Y. Denkers, Jitender P. Dubey, Jean-François Dubremetz, Jean E. Feagin, David J.P. Ferguson, Barbara A. Fox, Ricardo T. Gazzinelli, Uwe Gross, Marc-Jan Gubbels, Sandra K. Halonen, Omar S. Harb, Fiona L. Henriquez, Andrea Honda, Christopher A. Hunter, Damien Jacot, Farzana Khaliq, Imtiaz A. Khan, Kami Kim, Laura J. Knoll, Paul Latkany, Maryse Lebrun, David S. Lindsay, Carsten G.K. Lüder, Rima McLeod, Markus Meissner, José G. Montoya, Dana G. Mordue, Silvia N.J. Moreno, Naomi Morrissette, Jeremi Mullins, Eri Ochiai, Douglas A. Pace, Marilyn Parsons, Lucas Borges Pereira, Eskild Petersen, Sheela Prasad, Joshua B. Radke, Michael L. Reese, Utz Reichard, Craig W. Roberts, David S. Roos, Qila Sa, Frank Seeber, Lilach Sheiner, L. David Sibley, Anthony P. Sinai, Dominique Soldati-Favre, Boris Striepen, Emily Su, Chunlei Su, William J. Sullivan, Yasuhiro Suzuki, Stanislas Tomavo, Tadakimi Tomita, Christine Van Tubbergen, Gary Ward, Jonathan M. Wastling, Louis M. Weiss, Michael W. White, Dong Xia, and Robert Yolken

Published
2014

3. Contributors

Author

James W. Ajioka, Daniel Ajzenberg, James Alexander, Takashi Asai, Michael S. Behnke, John Boothroyd, Barbara Butcher, Dominique Buzoni-Gatel, David J. Bzik, Vern B. Carruthers, Marie-France Cesbron-Delauw, Kshitiz Chaudhary, Marie Laure Dardé, Eric Denkers, Wanderley de Souza, Robert G.K. Donald, J.P. Dubey, Jean-François Dubremetz, Joe Dan Dunn, Adrian Esquivel, Jianmin Fang, Jean E. Feagin, David J.P. Ferguson, Barbara A. Fox, Ricardo T. Gazzinelli, Uwe Gross, Sandra Halonen, Lloyd H. Kasper, Imtiaz A. Khan, Kami Kim, Paul Latkany, Maryse Lebrun, Oliver Liesenfeld, David S. Lindsay, Rima McLeod, Kildare Miranda, Silvia N.J. Moreno, Dorota Nowakowska, Marilyn Parsons, Eskild Petersen, Jay R. Radke, Utz Reichard, Craig W. Roberts, Peter Rohloff, Dick Schaap, L. David Sibley, Judith Smith, Dominique Soldati, Boris Striepen, William J. Sullivan, Yasuhiro Suzuki, Thomas J. Templeton, Stanislas Tomavo, Arno N. Vermeulen, Xisheng Wang, Jonathan M. Wastling, Louis M. Weiss, Xiangshu Wen, and Michael W. White

Published
2007

4. A sensitive, Semi-quantitative Direct PCR-RFLP Assay for Simultaneous Detection of Five Cryptosporidium Species in Treated Drinking Waters and Mineral Wat

Author

Jonathan M. Wastling, Huw V. Smith, C.A. Paton, B.M. Campbell, and R.A.B. Nichols

Subjects
Chromatography, animal diseases, Cryptosporidium, Biology, Natural mineral, biology.organism_classification, DNA extraction, Cryptosporidium species, Mineral water, Environmental chemistry, parasitic diseases, Lysis buffer, Restriction fragment length polymorphism, Semi quantitative
Abstract

Publisher Summary The purpose of this chapter is to describe a sensitive, semi-quantitative direct PCR-RFLP assay for simultaneous detection of five Cryptosporidium species in treated drinking waters and mineral waters. A protocol for maximizing DNA extraction from oocysts was followed. DNA was extracted from pre-determined numbers of oocysts suspended either in Polymerase Chain Rreaction (PCR) or in Lysis Buffer (LB). The evaluation of the semi-quantitative assay was performed with small numbers of Iowa isolate oocysts seeded into concentrates of mineral water. Four commercially available, still natural mineral waters containing a range of Total Dissolved Salts (TDS), were selected for the study Cryptosporidium oocysts trapped on polycarbonate filters and identified by epifluorescence microscopy were subjected to direct DNA extraction followed by PCR-RFLP analysis. The UK Standard Operating Protocols were used to concentrate oocysts from drinking water samples.

Published
2003

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