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The utilization of carbon dots (CDs) in agriculture to enhance plant growth has gained significant attention, but the data remains fractionated. Systematically integrating existing data is needed to identify the factors driving the interactions between CDs and plants and strategically guide future research. Articles reporting on CDs and their effects on plants were searched based on inclusion and exclusion criteria, resulting in the collection of 71 articles comprising a total of 2564 data points. The meta-analysis reveals that the soil and foliar application of red-emitting bio-derived CDs at a low concentration (<10 ppm) leads to the most beneficial effects on plant growth. Random forest and gradient boosting algorithms revealed that the size and dose of CDs were important factors in predicting plant responses across multiple aspects (CDs properties, plant properties, environmental factors, and experimental conditions). Specifically, smaller sizes are more favorable to growth indicators (GI) below 6 nm, nutrient and quality (NuQ) at 3-6 nm, photosynthesis (PSN) below 7 nm, and antioxidant responses (AR) below 5 nm. Overall, our analysis of existing data suggests that CDs applications can significantly improve plant responses (GI, NuQ, PSN, and AR) by 10-39 %. To unlock the full potential of CDs, customized synthesis techniques should be employed to meet the specific requirements of different crops and climate condition. For example, we recommend the synthesis of small CDs (<7 nm) with emission peak values falling within the range of 405-475 and 610-670 nm to enhance plant growth. The global prediction of plant responses to CDs application in future scenarios have shown significant improvements ranging from 17 to 58 %, suggesting that CDs have widespread applicability. This novel understanding of the impact of CDs on plant response provides valuable insights for optimizing the application of these nanomaterials in agriculture., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Recent research has highlighted the ecological risk posed by microplastics (MPs) from mulching film and heavy metals to soil organisms. However, most studies overlooked real environmental levels of MPs and heavy metals. To address this gap, pristine and aged polyethylene (PE) mulching film-derived MPs (PMPs, 500 mg/kg; AMPs, 500 mg/kg) were combined with cadmium (Cd, 0.5 mg/kg) to assess the acute toxicity to earthworms and investigate associated molecular mechanisms (oxidative stress, osmoregulation pressure, gut microbiota, and metabolic responses) at environmentally relevant concentrations. Compared to Cd alone and Cd + PMPs treatments (11.15 ± 4.19 items/g), Cd + AMPs treatment resulted in higher MPs bioaccumulation (23.73 ± 13.14 items/g), more severe tissue lesions, and increased cell membrane osmotic pressure in earthworms' intestines. Cd + AMPs induced neurotoxicity through elevated levels of glutamate and acetylcholinesterase. Earthworm intestines (0.98 ± 0.49 to 3.33 ± 0.37 mg/kg) exhibited significantly higher Cd content than soils (0.19 ± 0.01 to 0.51 ± 0.06 mg/kg) and casts (0.15 ± 0.01 to 0.25 ± 0.05 mg/kg), indicating PE-MPs facilitated Cd transport in earthworms' bodies. Metabolomic analysis showed Cd + AMPs exposure depleted energy and nucleotide metabolites, disrupted cell homeostasis more profoundly than Cd and Cd + PMPs treatments. Overall, co-exposure to AMPs + Cd induced more severe neurotoxicity and disruption of homeostasis in earthworm than Cd and PMPs + Cd treatments. Our study, using Cd and MPs with environmental relevance, underscores MPs' role in amplifying Cd accumulation and toxicity in earthworms., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

In wastewater-based epidemiology (WBE), wastewater loads are commonly reported as a per capita value. Census population counts are often used to obtain a population size to normalise wastewater loads. However, the methods used to calculate the population size of wastewater treatment plants (WWTPs) from census data are rarely reported in the WBE literature. This is problematic because the geographical extents of wastewater catchments and census area units rarely align perfectly with each other and exist at different spatial scales. This complicates efforts to estimate the number of people serviced by WWTPs in these census area units. This study compared four geospatial methods to combine wastewater catchment areas and census area units to calculate the census population size of wastewater treatment plants. These methods were applied nationally to WWTPs across New Zealand. Population estimates varied by up to 73 % between the methods, which could skew comparisons of per capita wastewater loads between sites. Variability in population estimates (relative standard deviation, RSD) was significantly higher in smaller catchments (r s  = -0.727, P < .001), highlighting the importance of method selection in smaller sites. Census population estimates were broadly similar to those provided by wastewater operators, but significant variation was observed for some sites (ranging from 42 % lower to 78 % higher, RSD = 262 %). We present a widely applicable method to calculate population size from census, which involves disaggregating census area units by individual properties. The results reinforce the need for transparent reporting to maintain confidence in the comparison of WBE across sites and studies., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Information on the occurrence of PFAS in aquatic matrices of countries with no PFAS manufacturing, e.g., New Zealand, is limited. Also, the fingerprint of PFAS along an urban water cycle, following water path from wastewater treatment plant (WWTP) effluent to treated drinking water has not been widely assessed. Hence, 38 long-, short-, ultrashort-chain PFAS and fluorinated alternatives (including precursors) were monitored in this study by collecting composite samples from two urban WWTPs of New Zealand and grab samples from the water bodies receiving the WWTPs' effluents and a drinking water treatment plant, whose source water received the effluent of one of the studied WWTPs. ∑PFAS at concentrations 0.1 - 13 ng/L were detected in all wastewater samples, including influents and different treatment stages of the two WWTPs (WW1 and WW2). The fate of most PFAS was similar in the two WWTPs, despite large differences in WWTPs' PFAS loads in the influents, serving populations (1.6 vs 0.16 million), total capacities (300 vs 54 million liters per day), and designs (aerobic and anoxic secondary treatment vs aerobic only). The fate of PFAS in WWTPs appeared to be driven by a range of processes. For instance, a simultaneous increase (41.6%) in short-chain perfluorohexanoic acid (PFHxA) concentrations and decrease (49.7%) in precursor 6:2 fluorotelomer sulfonate (6:2 FTS) concentrations after secondary biological treatment suggested possible transformation of 6:2 FTS into PFHxA during the treatment. In contrast, the reason behind an average decrease of 35% in ultrashort-chain perfluoropropionic acid (PFPrA) concentrations after treatment was unclear, and further studies are recommended. The concentrations of a linear isomer of long-chain perfluorosulfonic acid (PFOS-L) decreased (48%) in the effluent, possibly due to its partitioning to sludge. Although the concentrations of PFAS in coastal waters suggested that the WW1 effluent is a potential source of PFAS, earlier dispersion model and no detection of PFAS in the receiving waters of WW2 implied that other sources, such as septic systems, peripheral industries, and the airport, could also be contributing to PFAS in coastal waters. The source of ultrashort-chain PFPrA (5.5 ng/L) detected in the treated drinking water produced from that river was unclear. The monitoring results confirm incomplete removal of PFAS in WWTPs, indicate a possible transformation of unknown precursors present in wastewater into short-chain perfluoroalkylcarboxylic acids (PFCAs) during biological treatment, and reveal a possible accumulation of perfluoroalkylsulfonic acids (PFSAs) in the sludge, overall suggesting the circulation of PFAS in urban water systems., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Nanosized agrochemicals like nanofertilizers are being applied to soils. Adverse impacts of nanofertilizers on soil microflora were reported in past studies, but only considering a single application. Repeated applications are however more likely to occur in agriculture. We investigated effects of single versus repeated applications of a copper hydroxide nanofertilizer formulation (NFF) on soil enzyme activity and bacterial community. One or three applications were performed within 21 days to achieve same final level of Cu in soil (48 mg(Cu)/kg: the recommended dose of NFF). Besides, the active ingredient (i.e., copper hydroxide nanotubes (NT)) and dispersing agent (DA) of NFF, and an ionic fertilizer (i.e., CuSO 4 ) were examined. Fluorescein diacetate hydrolase (FDAse), N-acetylglucosaminidase (NAG), leucine aminopeptidase (LAP), and urease (URE) showed negligible changes in the activities between the control and DA treatment. Bacterial community abundance, composition and diversity exhibited similar phenomena. Exposures to copper hydroxide NFF and NT or CuSO 4 enhanced the activities of FDAse and NAG, weakened the activity of URE, and showed negligible changes in the LAP activity irrespective of single and repeated applications. Concentrations of NO 3 - -N and NH 4 + -N in soil were also affected by the application mode of NFF. More importantly, responses of soil bacterial community to copper hydroxide NFF were highly dependent on its application mode, whereas similar responses were observed in the CuSO 4 treatment regardless of single or repeated applications. This study provided new insights into environmental risk of copper hydroxide NFF that were ignored in previous studies using a single exposure., Competing Interests: Declaration of competing interest The authors declare no competing financial interest., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Per- and poly-fluoroalkyl substances (PFAS) have contaminated land and water at numerous sites worldwide that now require remediation. The most common approach for treating contaminated water currently relies on removal of PFAS by sorption. The spent sorbents loaded with PFAS can potentially be disposed of at landfills, provided the sorbed contaminants remain sequestered and certain risk criteria are met. Hence, it is essential that remediation sorbents (i) rapidly adsorb a large variety of PFAS under varying water chemistry conditions, and (ii) do not release the adsorbed PFAS in due course. This review aims at establishing the current state of knowledge about the potential release of PFAS that may occur during and after treatment. The scientific literature currently provides data for a very restricted range of long-chain PFAS. Our knowledge of the dynamics of PFAS adsorption processes on engineered sorbents is limited, and even less is known about their desorption processes. The sorption of PFAS can be strongly affected by changes in the solution pH, ionic strength and dissolved organic matter content, and the process is also subject to complex competition mechanisms in the presence of other PFAS as well as organic contaminants and inorganic salts. Several studies suggest that changes in one or several of these factors may trigger the release of PFAS from engineered sorbents. This phenomenon is more likely to occur for PFAS with shorter carbon chain lengths (

Poly- and perfluoroalkyl substances (PFASs) are synthetic chemicals, which are introduced to the environment through anthropogenic activities. Aqueous film forming foam used in firefighting, wastewater effluent, landfill leachate, and biosolids are major sources of PFAS input to soil and groundwater. Remediation of PFAS contaminated solid and aqueous media is challenging, which is attributed to the chemical and thermal stability of PFAS and the complexity of PFAS mixtures. In this review, remediation of PFAS contaminated soils through manipulation of their bioavailability and destruction is presented. While the mobilizing amendments (e.g., surfactants) enhance the mobility and bioavailability of PFAS, the immobilizing amendments (e.g., activated carbon) decrease their bioavailability and mobility. Mobilizing amendments can be applied to facilitate the removal of PFAS though soil washing, phytoremediation, and complete destruction through thermal and chemical redox reactions. Immobilizing amendments are likely to reduce the transfer of PFAS to food chain through plant and biota (e.g., earthworm) uptake, and leaching to potable water sources. Future studies should focus on quantifying the potential leaching of the mobilized PFAS in the absence of removal by plant and biota uptake or soil washing, and regular monitoring of the long-term stability of the immobilized PFAS., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Engineered silver sulfide nanoparticles (e-Ag 2 S-NPs) are used in industry and can be released into the environment. Besides e-Ag 2 S-NPs, transformed silver sulfide nanoparticles (t-Ag 2 S-NPs) from silver nanoparticles are more likely to be the form that is widely distributed in the environment. Both e-Ag 2 S-NPs and t-Ag 2 S-NPs may be ingested and get into human gastrointestinal tract (GIT) through trophic transfer, posing a potential threat to human health. Nevertheless, knowledge of chemical stability of t-Ag 2 S-NPs and e-Ag 2 S-NPs in the human GIT is very limited. Herein e-Ag 2 S-NPs and a series of t-Ag 2 S-NPs with different degrees of sulfidation were selected as models for exposure to the simulated human GIT including mouth, stomach and small intestine phases under fed and fasted conditions. Silver ions were detected in the simulated saliva, gastric and small intestine fluids when t-Ag 2 S-NPs or e-Ag 2 S-NPs were incubated in the simulated GIT, but the amount (e.g., < 20 μg) of silver ion in each phase accounted for < 0.2‰ (w/w) of the silver added (i.e., 100 mg). Silver species of the residual particulate from each phase of the simulated GIT with t-Ag 2 S-NPs or e-Ag 2 S-NPs were thus analyzed through a developed analytical method that could selectively, successively and efficiently dissolve and quantify AgCl, Ag(0), and Ag 2 S in particulates. Both e-Ag 2 S-NPs and fully sulfidized t-Ag 2 S-NPs were shown to be highly stable in the simulated human GIT. Conversely, partially sulfidized t-Ag 2 S-NPs primarily underwent transformations in the mouth phase relative to stomach and small intestine phases regardless of fed or fasted status, wherein AgCl and Ag 2 S were observed besides Ag(0). The amount of Ag 2 S in the mouth phase negatively (r = -0.99, p <  0.001) correlated with the sulfidation degree of initial t-Ag 2 S-NPs. This work improved our understanding of potential transformations of t-Ag 2 S-NPs in the simulated human GIT, providing valuable information for future researches on evaluating health risks of ingested Ag 2 S-NPs., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Diffusely contaminated soils often remain untreated as classical remediation approaches would be disproportionately expensive. Adding compost can accelerate the biodegradation of organic contaminants and adding biochar can immobilize contaminants through sorption. The combined use of compost and biochar to reduce polycyclic aromatic hydrocarbon (PAH) and NSO-substituted PAH contamination has, however, not previously been systematically investigated. We have therefore investigated the processes involved (i) through sorption batch experiments, (ii) by monitoring changes in bacterial, fungal and archaeal communities using denaturing gradient gel electrophoresis, and (iii) through degradation experiments with fluorene, phenanthrene, pyrene, carbazole, dibenzothiophene, and dibenzofuran. Sorption coefficients for organic contaminants in soils increased tenfold following 10% compost addition and up to a hundredfold with further addition of 5% biochar. The rate of PAH and NSO-PAH degradation increased up to twofold following compost addition despite increased sorption, probably due to the introduction of additional microbial species into the autochthonous soil communities. In contrast, degradation of PAHs and NSO-PAHs in soil-compost-biochar mixtures slowed down up to tenfold due to the additional sorption, although some degradation still occurred. The combined use of biochar and compost may therefore provide a strategy for immobilizing PAHs and NSO-PAHs and facilitating degradation of remaining accessible contaminant fractions., (Copyright © 2017 Elsevier B.V. All rights reserved.)

The study investigates the role of biochar and/or compost in mitigating the toxic effects of pyrene in soils using reproduction of nematodes and porewater concentration as measures of pyrene toxicity and bioavailability, respectively. Two soils were spiked with increasing levels of pyrene to achieve a concentration-response relationship for the reproduction of Caenorhabditis elegans. The observed EC50 values (pyrene concentration causing 50% inhibition of reproduction) were 14mg/kg and 31mg/kg (dry mass) for these soils, corresponding to equilibrium porewater concentrations of 37μg/L and 47μg/L, respectively. Differences in organic carbon content were not sufficient to explain the variability in toxicity between the different soils. Soils causing a significant inhibition of reproduction were further amended with 10%-compost, 5%-biochar, or both, and the effects on reproduction and porewater concentration determined. Combined addition of compost and biochar was identified as the most effective strategy in reducing pyrene concentration in soil porewater, which was also partly reflected in soil toxicity. However, porewater concentrations predicted only 52% of pyrene toxicity to nematodes, pointing to particle-bound or dietary exposure pathways. Capsule: Amending pyrene-spiked soil with biochar and compost effectively reduced pyrene porewater concentrations and toxicity to nematodes, which were significantly related., (Copyright © 2017 Elsevier B.V. All rights reserved.)

The surface area and pore volume of carbonaceous materials, which are commonly determined by N 2 and/or CO 2 gas-physisorption, are important parameters when describing environmental processes such as adsorption. Their measurement requires prior degassing of samples, which can change the nature of the material. Current guidelines for biochar characterization recommend different degassing temperatures. To investigate how degassing temperatures affect gas-physisorption we systematically degassed a range of materials (four biochars, carbon nanotubes, and Al 2 O 3 reference material) at different temperatures (105, 150, 200, 250 and 300°C; for ≥14h each). Degassing temperatures had no effect on Al 2 O 3 or carbon nanotubes but the measured surface areas and pore volumes of biochars increased by up to 300% with degassing temperature. An equation is presented for predicting surface area obtained at different degassing temperatures. Elemental analysis and results from sorption batch experiments suggest that surface area and pore volume may increase as biochar components volatilize during degassing. Our results showed that degassing temperatures change material properties and influence gas-physisorption measurements, and therefore need to be standardized. These results may also apply to the characterization of other complex materials, including carbon nanotubes coated with natural organic matter and fouled activated carbon., (Copyright © 2016 Elsevier B.V. All rights reserved.)

The effect of functionalization on the sorption behavior of carbon nanotubes (CNTs) remains poorly understood, especially when combined with other factors affecting dispersion. The sorption behavior of a series of functionalized CNTs towards pyrene has therefore been systematically evaluated over a wide range of concentrations and dispersion scenarios. When studied as purchased (in the absence of humic acids and sonication treatment), sorption isotherms showed that OH-, COOH- and NH2-CNTs exhibited significantly different sorption affinity for pyrene. Sonication greatly increased both the sorption affinity and the maximum capacity of all types of functionalized CNTs, to an extent that overwhelmed the differences initially observed (increase of up to 1.5 orders of magnitude lead to log Kd values close to 9 L/kg). Results demonstrate that a significant proportion of the CNT surface was unavailable to pyrene prior to sonication. The presence of humic acids enhanced dispersion but decreased sorption, especially when combined with sonication. Sorption affinity, however, remained very high in all cases (log Kd>7.5 L/kg), suggesting that CNTs can act as strong sorbents under a wide range of conditions., (Copyright © 2014 Elsevier B.V. All rights reserved.)