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Thin films of Ni:ZnO were successfully synthesized by sol-gel spin coating system for 0.5 % and 2.5 % Ni doping concentration on FTO coated substrate. The synthesized films were annealed at 540 °C for 4 h. The annealed thin films were characterized for its electrical, optical and chemical characteristics using UV–Vis, Micro Raman, and FTIR spectroscopy, respectively. The UV–Vis spectra analysis reveals that the energy band gap of deposited films found to be 3.58 eV and 3.51 eV for 0.5 % and 2.5 % Ni:ZnO thin films, respectively. Two significant characteristic peaks identified at 424 cm−1 and 563 cm−1 in Raman spectra. These peaks are attributed to E2high and LO(A1 and E1) modes, which confirms the hexagonal wurtzite phase of Ni:ZnO thin films. Furthermore, the absorption peaks observed at 530 cm−1 and 635 cm−1 in the FTIR spectra are attributed to the characteristic stretching vibrational modes of Zn-O and Ni-O bonds, respectively.

Plastic waste has become a significant global ecological concern due to its substantial harmful impacts. They are eventually colonized and transformed by diverse microbial communities known as the ‘‘plastisphere”. Only a limited group of microbes have been identified so far due to the prevalence of non-culturable strains that inhabit polymer substrates. We investigated the community diversity and predictive functionality of the plastisphere microbiota using a high-throughput next-generation sequencing (NGS) approach to unveil their plastic degrading potential. Samples were collected from four different plastic-enriched disposal sites adjacent to Kolkata, West Bengal, India, followed by processing, gDNA extraction, library preparation, and sequencing. The most prevalent phyla were Proteobacteria, followed by Bacteroidetes, Actinobacteria, Cyanobacteria, Firmicutes, and Verrucomicrobia. The functional profiling revealed that genes associated with metabolism, cellular processes, and signalling were most prevalent, followed by poorly characterized information storage and processing. In this study, we predicted the existence of a beneficial microbiome associated with bioremediation and plastic degradation, which suggests the potential utilization of plastics as primary carbon sources. Our findings also highlighted the existence of promising microbial enzymes associated with the biodegradation of several plastic substrates.Furthermore, our research unveiled the enriched distribution of beneficial microbiome in the studied metagenome, which offers a diverse prospect. This investigation establishes a connection between the structure of microbial communities and diverse genes actively engaged in the biodegradation of plastic waste within plastic disposal sites. These beneficial degraders can be investigated further for broad-spectrum applications in plastic bioremediation. Subsequent explorations of their plastic degrading enzymes will provide profound contributions to plastic pollution mitigation.

Purpose: The COVID-19 pandemic has caused 1.4 million deaths globally and is associated with a 3–4 times increase in 30-day mortality after a fragility hip fracture with concurrent COVID-19 infection. Typically, death from COVID-19 infection occurs between 15 and 22 days after the onset of symptoms, but this period can extend up to 8 weeks. This study aimed to assess the impact of concurrent COVID-19 infection on 120-day mortality after a fragility hip fracture. Methods: A multi-centre prospective study across 10 hospitals treating 8% of the annual burden of hip fractures in England between 1st March and 30th April, 2020 was performed. Patients whose surgical treatment was payable through the National Health Service Best Practice Tariff mechanism for “fragility hip fractures” were included in the study. Patients’ 120-day mortality was assessed relative to their peri-operative COVID-19 status. Statistical analysis was performed using SPSS version 27. Results: A total of 746 patients were included in this study, of which 87 (11.7%) were COVID-19 positive. Mortality rates at 30- and 120-day were significantly higher for COVID-19 positive patients relative to COVID-19 negative patients (p

Summary: Despite substantial progress in our understanding of the players involved and the regulatory mechanisms controlling the initiation and elongation steps of transcription, little is known about the recruitment of elongation factors at promoter-proximal regions for the initiation-to-elongation transition. Here, we show evidence that human TFIID, which initiates pre-initiation complex (PIC) assembly, contributes to regulating the recruitment of super-elongation complex (SEC) components at the promoter-proximal region through interactions among selective TAF and SEC components. In vitro direct interactions, coupled with cell-based assays, identified an important poly-Ser domain within SEC components that are involved in their interaction with TFIID. DNA template-based recruitment assays, using purified components, further show a direct role for poly-Ser domain-dependent TFIID interaction in recruiting SEC components on target DNA. Consistently, ChIP and RNA analyses have shown the importance of this mechanism in TFIID-dependent SEC recruitment and target gene expression within mammalian cells. : Yadav et al. show a role for human TFIID in recruiting SEC at the promoter-proximal region through poly-Ser domain-dependent interaction between selective SEC components and TFIID subunits for the transcriptional activation of target genes. Keywords: transcription, TFIID, super elongation complex, P-TEFb, AF9, poly-Ser, TAF6, pause, elongation

De-oiled groundnut oil cake (GOC) was bio-processed through solid state fermentation (SSF) for 15 days at 34 ± 1 °C by a tannase producing yeast, Pichia kudriavzevii (GU939629) isolated from the gut of a freshwater carp, Cirrhinus cirrhosus. SSF of GOC was effective in significantly (P