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Your search keyword '"Laboratoire d'acoustique de l'université du Maine (LAUM) ' showing total 2 results
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2 results on '"Laboratoire d'acoustique de l'université du Maine (LAUM) '

1. Diazonium modified gold microelectrodes onto polyimide substrates for impedimetric cytokine detection with an integrated Ag/AgCl reference electrode

Author

SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes ; Institut des Sciences Analytiques (ISA) ; CNRS - Université Claude Bernard - Lyon I (UCBL) - PRES Université de Lyon - École Normale Supérieure (ENS) - Lyon - CNRS - Université Claude Bernard - Lyon I (UCBL) - PRES Université de Lyon - École Normale Supérieure (ENS) - Lyon - Nanobioengineering Group-IBEC ; University of Barcelona - University of Barcelona - Laboratoire TransDiag ; Etablissement Français du Sang - Etablissement Français du Sang, Laboratoire d'acoustique de l'université du Maine (LAUM) ; CNRS - Université du Maine, Clinical Physiology Institute ; Consiglio Nazionale Ricerche (CNR), Centre Nacional de Microelectronica (CNM-IMB) ; Centre Nacional de Microelectronica (CNM-IMB), the European Communities Seventh Framework Programme (FP7/2007-2013 grant agreement No. 248763 SensorART-IP , and FP7-PEOPLE-2012-IRSES grant agreement No. 318053 SMARTCANCERSENS; SPS (NUKP.SFPP984173) NATO project; the Consolider-Ingenio 2010 programme (Nanoselect project CSD2007-00041), Baraket, Abdellatif, Lee, Michael, Zine, Nadia, Sigaud, Monique, Yaakoubi, Nourdin, Trivella, Maria Giovanna, Zabala, Miguel, Bausells, Joan, Jaffrezic-Renault, Nicole, Errachid, Abdelhamid, SIMS - Surfaces-(bio)Interfaces - Micro & Nano Systèmes ; Institut des Sciences Analytiques (ISA) ; CNRS - Université Claude Bernard - Lyon I (UCBL) - PRES Université de Lyon - École Normale Supérieure (ENS) - Lyon - CNRS - Université Claude Bernard - Lyon I (UCBL) - PRES Université de Lyon - École Normale Supérieure (ENS) - Lyon - Nanobioengineering Group-IBEC ; University of Barcelona - University of Barcelona - Laboratoire TransDiag ; Etablissement Français du Sang - Etablissement Français du Sang, Laboratoire d'acoustique de l'université du Maine (LAUM) ; CNRS - Université du Maine, Clinical Physiology Institute ; Consiglio Nazionale Ricerche (CNR), Centre Nacional de Microelectronica (CNM-IMB) ; Centre Nacional de Microelectronica (CNM-IMB), the European Communities Seventh Framework Programme (FP7/2007-2013 grant agreement No. 248763 SensorART-IP , and FP7-PEOPLE-2012-IRSES grant agreement No. 318053 SMARTCANCERSENS; SPS (NUKP.SFPP984173) NATO project; the Consolider-Ingenio 2010 programme (Nanoselect project CSD2007-00041), Baraket, Abdellatif, Lee, Michael, Zine, Nadia, Sigaud, Monique, Yaakoubi, Nourdin, Trivella, Maria Giovanna, Zabala, Miguel, Bausells, Joan, Jaffrezic-Renault, Nicole, and Errachid, Abdelhamid

Abstract

International audience, This paper describes a simple technique for the fabrication of a gold microelectrode array with an integrated Ag/AgCl reference microelectrode based on polyimide (PI) which is a flexible and biocompatible thermoplastic substrate. The investigated fabrication technology was based on soft lithography which involved a combination of replica molding (RM) and patterning of self-assembled monolayers (SAMs), through microcontact printing (μCP). From a fabricated silicon microelectrode mold, an elastomeric stamp of the microelectrodes was replicated in polydimethylsiloxane (PDMS) and inked with octadecanethiol (ODT) for patterning of the gold surface. The non-patterned gold was then removed by selective chemical etching leaving very well defined gold microelectrodes with a high resolution. Finally, the integrated Ag/AgCl reference microelectrode was fabricated by silver electro-deposition onto the gold microelectrode. The developed biosensor was then tested for a medical application by immobilizing both monoclonal antibodies (mAb); anti-human interleukin-10 (IL-10) and anti-human tumor necrosis factor-alpha (TNF-α) onto different working electrodes. Functionalization of the gold microelectrodes was made with carboxyl diazonium through cyclic voltammetry (CV). The multiple detection of the corresponding cytokines was verified by fluorescence, whilst electrochemical impedance spectroscopy (EIS) analysis quantified a detectable IL-10 cytokine concentration from 1 to 15 pg/mL. This confirmed the biorecognition between the anti-human mAb (IL-10 and TNF-α) and of its corresponding cytokine. The obtained results show that the mAb's were efficiently immobilized and the proteins for human IL-10 were detectable at weak concentrations. The electrochemical measurements were applied on the manufactured gold microelectrodes for ∼9 h. This shows a high resistance of the microelectrodes for long-lasting measurements and, therefore, highlights the good adhesion of gold-PI. The results prese

2. SU-8 waveguiding interferometric micro-sensor for gage pressure measurement

Author

Laboratoire d'acoustique de l'université du Maine (LAUM) ; CNRS - Université du Maine, Groupe matière condensée et matériaux (GMCM) ; CNRS - Université de Rennes 1, Physique des atomes, lasers, molécules et surfaces (PALMS) ; CNRS - Université de Rennes 1, Laboratoire de Photonique Quantique et Moléculaire (LPQM) ; CNRS - École normale supérieure (ENS) - Cachan, Pelletier, Nicolas, Bêche, Bruno, TAHANI, Najat, Zyss, Joseph, Camberlein, Lionel, Gaviot, Etienne, Laboratoire d'acoustique de l'université du Maine (LAUM) ; CNRS - Université du Maine, Groupe matière condensée et matériaux (GMCM) ; CNRS - Université de Rennes 1, Physique des atomes, lasers, molécules et surfaces (PALMS) ; CNRS - Université de Rennes 1, Laboratoire de Photonique Quantique et Moléculaire (LPQM) ; CNRS - École normale supérieure (ENS) - Cachan, Pelletier, Nicolas, Bêche, Bruno, TAHANI, Najat, Zyss, Joseph, Camberlein, Lionel, and Gaviot, Etienne

Abstract

International audience, The authors present a successful modeling, realization and characterization of a new micro-sensor based on a convenient optical principle, namely an integrated Mach-Zehnder interferometer (MZI). This MZI device is designed with a view to measuring pressure disturbances due to optical path variations. Such a system is arranged in order to work in intensity modulation scheme. Moreover, the MZI is made up of straight and bent rib optical waveguides composed of SU-8 polymer. The mainstay of the device is based on differential measurements performed by a sensing arm arranged with a micromachined membrane and actuated by a given pressure disturbance, while the second arm of the interferometer is considered as a reference one. The main parameters of each element are given by way of two modeling approaches: an optical modeling with a semi-vectorial finite difference method together with a conformal transformation, and a mechanical modeling with a finite-element method associated to the mechanical theory of membranes. So, as the pressure to be measured is applied upon the diaphragm, an optical path variation of the acting arm is induced. After the combination of both signals, the variation at the output of the system is measured. A prototype is characterized by way of a micro-optical injection bench specifically designed to allow an efficient end-fire coupling into the waveguides.

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