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Background: Liver cancer (LC) is a major global health issue, with China accounting for nearly half of global cases. Key contributors include chronic hepatitis B virus (HBV) infection, aflatoxin exposure, and modifiable lifestyle risk factors. With significant global implications, China's high LC incidence and mortality underscore the need for focused research. Methods: This study analyses age- and sex-specific LC trends and risk factors across China's 34 provincial-level administrative regions using GBD 2021 data. LC metrics examined include incidence, prevalence, mortality, disability-adjusted life years (DALYs), years lived with disability (YLDs), and years of life lost(YLLs). We also examined the relationship between these metrics and gross-domestic-product-per-capita (GDPPC). An attentive deep-learning pipeline was developed to provide long-term scenario forecasts with scenarios simulation. Findings: Between 1990 and 2021, LC incidence and prevalence in China escalated (except in Fujian, Shanghai, and Zhejiang), reaching 196,637 new cases and 265,539 total cases in 2021, predominantly affecting individuals aged 45+ years. In 2021, LC attributed to HBV recorded the highest age-standardised incidence rate (ASIR), whereas increases in ASIR were most pronounced for cases due to alcohol use and non-alcoholic steatohepatitis (NASH) from 1990-to-2021. The national age-standardised death rate (ASDR) declined from 10.8 to 8.4 per 100,000 from 1990 to 2021. Notably, hepatoblastoma showed the most significant drop in ASDR, plummeting by 79.7%, with subsequent declines in LC due to HBV, HCV, and alcohol use. Conversely, LC due to NASH saw a marginal increase of 0.8%. Trends in age-standardised DALYs, YLDs, and YLLs moderately mirrored those of the ASDR. Men, particularly those over 20 years old, faced higher LC burdens across all provincial regions compared to women. Provincially, the lowest LC burdens were observed in high-GDPPC regions, such as Beijing and Shanghai. In 2021, smoking emerged as the leading risk factor for LC-related mortality and disability in men, especially in HBV-associated cases. Men under 20 years displayed distinct patterns, with high alcohol use being the predominant fatal risk factor. For women, inappropriate drug use was the foremost risk factor for LC-related mortality and disability, especially in HCV-related cases. Without targeted interventions, forecasts from 2021 to 2040 predict declines in age-standardized LC rates: incidence by 9.5%, mortality by 4.7%, DALYs by 6.5%, YLLs by 4.3%, and YLDs by 13%. However, the incidence of LC due to alcohol use is expected to significantly increase among those under 20 years old in all provinces except Tibet, indicating a worrying trend towards younger onset. Targeted interventions, including tobacco control, appropriate medication use, alcohol use restriction, and regular physical activity, are projected to significantly reduce LC-attributed health losses in both sexes. Notably, reductions in mortality, DALYs, YLDs, and YLLs rates for LC in men are primarily expected through tobacco control; while in women, appropriate medication use will play the most critical role. Interpretation: Our findings indicate a rapidly increasing LC burden, particularly for cases attributable to HBV, in China. The scenario-based forecasting model provides compelling evidence that can encourage national and provincial stakeholders to develop effective interventions tailored to their specific populations.

Characterization of defects in large 3D printed metals is critical but challenging. This study reports on the X-ray micro-computed tomography (micro-CT) examination of porosities in large-volume 3D-printed and heat-treated titanium (Ti–6Al–4V) alloys, together with their tensile properties and failure mechanisms. Titanium alloy powders were analyzed using scanning electron microscopy (SEM). Laser powder bed fusion (L-PBF) was used to print titanium alloy specimens vertically and horizontally, followed by stress-relieved heat treatment. Micro-CT imaging was performed on printed specimens of 10 × 20 mm3 (diameter × length) to determine their porosities, pore locations and size distributions using an industrial micro-CT system and relevant imaging software. Tensile testing of the processed specimens was conducted to determine their mechanical properties. Optical microscopy and SEM were used to examine the tension-induced failure mechanisms. The results show that porosities, pore sizes and locations were influenced by the build direction, resulting in different mechanical properties. Horizontal printing achieved higher tensile modulus, strength, ductility, resilience and toughness than vertical printing. Heat treatment did not change porosities in horizontally built specimens, but slightly reduced porosities for vertically built ones by 10%. This led to most mechanical properties nearly unchanged for the horizontally printed specimens but remarkably increased yield and tensile strength, and resilience, for the vertically printed ones. All tension-induced fractured surfaces contained pores, possible indicators of failure origins, which should be diminished in advanced processes for higher mechanical reliability.

A distributed high-speed data acquisition and monitoring system for the RF negative ion source at Huazhong University of Science and Technology (HUST) is developed, which consists of data acquisition, data forwarding and data processing. Firstly, the data acquisition modules sample physical signals at high speed and upload the sampling data with corresponding absolute-time labels over UDP, which builds the time correlation among different signals. And a special data packet format is proposed for the data upload, which is convenient for packing or parsing a fixed-length packet, especially when the span of the time labels in a packet crosses an absolute second. The data forwarding modules then receive the UDP messages and distribute their data packets to the real-time display module and the data storage modules by PUB/SUB-pattern message queue of ZeroMQ. As for the data storage, a scheme combining the file server and MySQL database is adopted to increase the storage rate and facilitate the data query. The test results show that the loss rate of the data packets is within the range of 0–5% and the storage rate is higher than 20 Mbps, both acceptable for the HUST RF negative ion source.

The death of Sertoli cells (SCs) under condition of heat stress (HS) affects spermatogenesis and is associated with impaired function of the blood-testis barrier (BTB). The fatty acid arachidonic acid (AA) is essential for the maintenance of cellular function. However, excessive release of AA during HS may adversely affect the reproductive function. The molecular mechanisms through which AA modulates the BTB in SCs are unclear. In this study, we found that 100 µM AA damaged testicular morphology and accelerated SC apoptosis during HS, reducing the stability of tight junction proteins (TJPs), shown by measurement of the levels of Claudin 11, 5, Occludin, and trans-epithelial electrical resistance (TEER). It was also found that AA adversely affected TJPs by increasing the accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA), activating p38 mitogen-activated protein kinases (P38 MAPK) and reducing mitochondria DNA (mtDNA) and the expression of mitochondrial complexes I and III. In contrast, pretreatment with SB203508 (a P38 MAPK inhibitor), Rotenone (an inhibitor of complex I) and Antimycin A1 (an inhibitor of complex III) reversed TJPs degradation induced by AA. Interestingly, pretreatment of cells with 10 µM Baicalein, a 12/15 lipoxygenase (12/15-LOX) -dependent inhibitor of AA production, protected against AA-induced TJPs degradation, restored mitochondrial function, and reduced apoptosis. These results suggested an intriguing link between the induction of TJPs degradation induced by AA overload and mitochondrial antioxidant function during HS, which was found to be regulated by the mitochondrial complex-ROS-P38 MAPK axis.

Machining-induced edge chipping damage represents a common challenge in ceramic applications. This paper reports on responses of zirconia materials with porous and dense microstructures to edge chipping damage induced in conventional and ultrasonic vibration-assisted diamond machining. The machining-induced damage was evaluated using optical and scanning electron microscopies. The results show that edge chipping damage produced in these processes was associated with brittle fracture and depends on the material microstructure and the vibration amplitude. Pre-sintered porous zirconia with a high brittleness index yielded significantly larger edge chipping damage than sintered dense zirconia with a low index in these processes. Ultrasonic machining at an optimal vibration amplitude minimized the scale of brittle fracture at the micro level, and thus significantly diminished edge chipping damage in zirconia materials with distinct microstructures. The investigation underpins the transition from conventional to ultrasonic vibration-assisted machining for manufacturing of ceramics to achieve better product quality.

We have reported that of the 10 most commonly used adeno-associated virus (AAV) serotype vectors, AAV6 is the most efficient in transducing primary human hematopoietic stem cells (HSCs) in vitro, as well as in vivo. More recently, polyvinyl alcohol (PVA), was reported to be a superior replacement for human serum albumin (HSA) for ex vivo expansion of HSCs. Since HSA has been shown to increase the transduction efficiency of AAV serotype vectors, we evaluated whether PVA could also enhance the transduction efficiency of AAV6 vectors in primary human HSCs. We report here that up to 12-fold enhancement in the transduction efficiency of AAV6 vectors can be achieved in primary human HSCs with PVA. We also demonstrate that the improvement in the transduction efficiency is due to PVA-mediated improved entry and intracellular trafficking of AAV6 vectors in human hematopoietic cells in vitro, as well as in murine hepatocytes in vivo. Taken together, our studies suggest that the use of PVA is an attractive strategy to further improve the efficacy of AAV6 vectors. This has important implications in the optimal use of these vectors in the potential gene therapy and genome editing for human hemoglobinopathies such as β-thalassemia and sickle cell disease.

Purpose: To observe the therapeutic effect of interventional embolization and haemorrhage control in multiple trauma patients with a major abdominal or pelvic injury. Methods: Data of 160 multiple trauma patients with a major abdominal or pelvic injury were retrospectively analyzed. They were admitted into the Department of Emergency of the First Affiliated Hospital of Zunyi Medical College from October 2013 to April 2016. Eighty-seven patients who received emergent intervention for embolization and haemorrhage control were set as group A, including 72 males and 15 females, with an average age of (39.32 ± 14.0) years. Patients underwent emergent intervention for embolization and hemostasis. The other 73 patients who received traditional surgeries were set as group B, including 62 males and 11 females, with an average age of (38.48 ± 13.12) years. The time from admission to emergency intervention, the time of interventional embolization, transfusion during hospitalization, length of stay and prognosis were observed. The whole treatment and prognosis were compared between group A and group B. Results: In group A, the average time from admission to intervention exploration was (132.05 ± 86.80) min, the average operation time was (149 ± 49.69) min, the average hospitalization time was (18.37 ± 4.71) days, the average amount of RBC transfusion during hospitalization was (7.2 ± 4.33) units, and the mortality was 4.60% (4 patients died). The corresponding data in group B were respectively (138.95 ± 82.49) min, (183 ± 52.39) min, (22.72 ± 6.63) days, (12.23 ± 5.43) units, and 9.59% (7 cases died). There was no statistical difference in the time from admission to operation between the two groups (p > 0.05), but there was statistical difference in operation time, RBC transfusion, hospitalization time, prognosis, and mortality between the two groups (all p

Rice kernel shape affects kernel quality (appearance) and yield (1000-kernel weight) and therefore is an important agronomic trait, but its inheritance is complicated. We identified a long-kernel rice chromosome segment substitution line (CSSL), Z741, derived from Nipponbare as a recipient and Xihui 18 as a donor parent. Z741 has six substitution segments distributed on rice chromosomes 3, 6, 7, 8 and 12 with an average replacement length of 5.82 Mb. Analysis of a secondary F2 population from a cross between Nipponbare and Z741 identified 20 QTLs for important agronomic traits. The kernel length of Z741 is controlled by a major QTL (qKL3) and a minor QTL (qKL7). Candidate gene prediction and sequencing indicated that qKL3 may be an allele of OsPPKL1, which encodes a protein phosphatase implicated in brassinosteroid signaling, and qKL7 is an unreported QTL. Finally, we validated eight QTLs (qKL3, qKL7, qRLW3-1, qRLW7, qPH3-1, qKWT3, qKWT7 and qNPB6) using three selected single- segment substitution lines (SSSLs), S1, S2 and S3. Also, we detected five QTLs (qKL6, qKW3, qKW7, qKW6 and qRLW6) in S1, S2 and S3, which were not found in the Nipponbare/Z741 F2 population. However, qNPB3, qNPB7 and qPL3 QTLs were not validated by the three SSSLs in 2019, suggesting that minor QTLs are susceptible to environmental factors. These results lay the foundation for studying the biodiversity of kernal length and molecular breeding of different kernel types.

Background: Abusive head trauma (AHT) is the leading cause of death in infants with traumatic brain injury (TBI). Early recognition of AHT is important for improving outcomes, but it can be challenging due to its similar presentations with non-abusive head trauma (nAHT). This study aims to compare clinical presentations and outcomes between infants with AHT and nAHT, and to identify the risk factors for poor outcomes of AHT. Methods: We retrospectively analyzed infants of TBI in our pediatric intensive care unit from January 2014 to December 2020. Clinical manifestations and outcomes were compared between patients with AHT and nAHT. Risk factors for poor outcomes in AHT patients were also analyzed. Results: 60 patients were enrolled for this analysis, including 18 of AHT (30%) and 42 of nAHT (70%). Compared with those with nAHT, patients with AHT were more likely to have conscious change, seizures, limb weakness, and respiratory failure, but with a fewer incidence of skull fractures. Additionally, clinical outcomes of AHT patients were worse, with more cases undergoing neurosurgery, higher Pediatric Overall Performance Category score at discharge, and more anti-epileptic drug (AED) use after discharge. For AHT patients, conscious change is an independent risk factor for a composite poor outcome of mortality, ventilator dependence, or AED use (OR = 21.9, P = 0.04) Conclusion: AHT has a worse outcome than nAHT. Conscious change, seizures and limb weaknesses but not skull fractures are more common in AHT. Conscious change is both an early reminder of AHT and a risk factor for its poor outcomes.

SCN4A mutations have been shown to be associated with myotonia, paramyotonia congenita, and periodic paralyses. More recently, loss-of-function variants in the SCN4A gene were also noted to be associated with rarer, autosomal recessive forms of congenital myasthenic syndrome and congenital myopathy. Diagnosis is challenging as the initial clinical presentation and histological features on muscle biopsies are non-specific. We report a Han Chinese patient presented with congenital myopathy with two missense SCN4A variants. The patient had an antenatal history of reduced fetal movements, polyhydramnios and a very preterm birth. At birth, she was noted to have low Apgar score, respiratory distress syndrome and hypotonia. Delayed motor development was noted in early childhood. Dysmorphic features such as an elongated face, dolichocephaly and high arched palate were present. At 16 years of age, the patient developed progressive muscle weakness and was wheelchair-bound by age 20. Muscle biopsy revealed non-specific changes only. Targeted hereditary myopathy panel testing by next generation sequencing revealed two previously unreported missense variants c.1841A > T p.(Asn614Ile) and c.4420G > A p.(Ala1474Thr) in the SCN4A gene. The clinical features of SCN4A-related congenital myopathy and myasthenic syndrome were reviewed. This case exemplifies the utility of next generation sequencing in the diagnosis of undifferentiated muscle disease.

Background/Purpose: Identifying child abuse is sometimes challenging due to its various presentations. To facilitate timely identification of critical or complex cases of physical abuse outside our child protection center, we established an outreach multidisciplinary team (OMDT) to support Kaohsiung City Government in 2014. The objective of this study was to describe our experience of OMDT services during a 6-year period and examine its role in assisting law enforcement. Methods: We retrospectively analyzed all OMDT cases from January 2014 to January 2020. Clinical characteristics and OMDT reports were reviewed. After inspection by our OMDT, cases were determined as indicating either a high risk or low risk of child abuse. Associations among clinical characteristics, radiographic findings, OMDT decisions and case outcomes including law enforcement and prosecution were examined. Results: Thirty-two cases (22 [68.8%] males and 10 [31.2%] females; mean age 24.2 months) received OMDT service, of whom 28 (87.5%) were admitted to the pediatric intensive care unit. The victims had an average of 2.2 types of wounds in 3.4 locations. The most common finding on radiography was subdural hemorrhage (18, 56.3%), followed by subarachnoid hemorrhage (31, 31.3%). Law enforcement was activated in 20 (64.5%) cases, and was only associated with the high-risk group as determined by the OMDT (p

Background: s: Hyperhomocysteinemia (HHcy) is one of risk factors for vascular cognitive impairment (VCI). GTP cyclohydrolase 1 (GCH1) deficiency is critical to oxidative stress in vascular dysfunction. The aim of this study was designed to examine whether HHcy induces VCI through GCH1 S-nitrosylation, a redox-related post-translational modification of cysteine. Methods: The VCI model was induced by feeding mice homocysteine thiolactone (HTL) for 16 consecutive weeks. The cognitive functions were evaluated by step-down avoidance test, passive avoidance step-through task test, and Morris water maze (MWM) test. Protein S-nitrosylation was assayed using a biotin-switch method. Results: In cell-free system, nitric oxide (NO) donor induced GCH1 protein S-nitrosylation and decreased GCH1 activity. In endothelial cells, HTL increased GCH1 S-nitrosylation, reduced tetrahydrobiopterin, and induced oxidative stress, which were attenuated by N-acetyl-cysteine, L-N6-1-Iminoethyl-lysine, mutant of GCH1 cysteine 141 to alanine (MT-GCH1) or gene deletion of inducible NO synthase (iNOS). Further, HTL incubation or iNOS overexpression promoted endothelial cellular senescence, but abolished by exogenous expression of MT-GCH1 or pharmacological approaches including N-acetyl-cysteine, L-sepiapterin, and tempol. In wildtype mice, long-term administration of HTL induced GCH1 S-nitrosylation and vascular stiffness, decreased cerebral blood flow, and damaged the cognitive functions. However, these abnormalities induced by HTL administration were rescued by enforced expression of MT-GCH1 or gene knockout of iNOS. In human subjects, GCH1 S-nitrosylation was increased and cognitive functions were impaired in patients with HHcy. Conclusion: The iNOS-mediated nitrosative stress induced by HTL drives GCH1 S-nitrosylation to induce cerebral vascular stiffness and cognitive impairments.

Background: Few studies explored the longitudinal link between early-life secondhand smoke (SHS) exposure and later alcohol initiation despite its risk for child behavioral difficulties. We examined the associations of the timing, level, and pattern of SHS exposure from pregnancy to childhood with early alcohol initiation and evaluated the sex differences in these associations., Methods: Data were from 16,440 participants of the Taiwan Birth Cohort Study conducted when the children were aged 6 months, 18 months, 3 years, 5.5 years, 8 years, and 12 years. Group-based trajectory modeling was applied to identified patterns of SHS exposure. A series of multiple logistic regression were conducted to examine study hypotheses., Results: Exposure to prenatal SHS was associated with an increased risk of early alcohol initiation (adjusted odds ratio [aOR] = 1.17, 95% confidence interval [CI] = 1.06, 1.30). Compared with the adolescents with a persistent-low-exposure trajectory, those who exhibited prenatal-high-decreasing (aOR = 1.18, 95% CI = 1.04, 1.35) or persistent-high-exposure (aOR = 1.27, 95% CI = 1.12, 1.45) patterns exhibited increased risks of early alcohol initiation. Those with higher cumulative levels of SHS exposure also exhibited an increased risk of early alcohol initiation (aOR = 1.03, 95% CI = 1.01, 1.04). Sex differences were also observed., Conclusions: Varying timing, levels, and longitudinal patterns of SHS exposure during early life had differential effects on early alcohol initiation, with the effects differing by sex. Targeting SHS exposure while considering the nature of exposure and sex differences could help prevent and curb alcohol use in adolescents., Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Research Question: Non-invasive preimplantation genetic testing for aneuploidies (niPGT-A) avoids the possible detrimental impact of invasive PGT-A on embryo development and clinical outcomes. Does cell-free DNA (cfDNA) from spent blastocyst culture medium (BCM) reflect embryonic chromosome status better than trophectoderm (TE) biopsy?, Design: In this study, 35 donated embryos were used for research and the BCM, TE biopsy, inner cell mass (ICM) and residual blastocyst (RB) were individually picked up from these embryos. Whole genome amplification (WGA) was performed and amplified DNA was subject to next-generation sequencing. Chromosome status concordance was compared among the groups of samples., Results: The WGA success rates were 97.0% (TE biopsy), 100% (ICM), 97.0% (RB) and 88.6% (BCM). Using ICM as the gold standard, the chromosomal ploidy concordance rates for BCM, TE biopsy and RB were 58.33% (14/24), 68.75% (22/32) and 78.57% (22/28); the diagnostic concordance rates were 83.33% (20/24), 87.50% (28/32) and 92.86% (26/28); and the sex concordance rates were 92.31% (24/26), 100% (32/32) and 100% (28/28), respectively. Considering RB the gold standard, the chromosome ploidy concordance rates for BCM and TE biopsy were 61.90% (13/21) and 81.48% (22/27); the diagnostic concordance rates were 71.43% (15/21) and 88.89% (24/27); and the sex concordance rates were 91.30% (21/23) and 100% (27/27), respectively., Conclusions: The results of niPGT-A of cfDNA of spent BCM are comparable to those of invasive PGT-A of TE biopsies. Modifications of embryo culture conditions and testing methods will help reduce maternal DNA contamination and improve the reliability of niPGT-A., (Copyright © 2022. Published by Elsevier Ltd.)

Objective: This study identified developmental patterns of handwashing habit formation during childhood and examined their associations with later COVID-19 preventive practices., Methods: We used data from the Taiwan Birth Cohort Study, which included 11,254 adolescents with complete data on childhood handwashing behavior and age-15 COVID-19 survey items. Bias-adjusted 3-step latent class analysis was used to test study hypotheses., Results: The rates of handwashing and mask-wearing during the pandemic were 63.8% and 93.8%, respectively. Five distinct patterns of handwashing habit formation were identified: early formation (14.89%), delayed formation (17.73%), gradual formation (42.98%), inconsistent formation (9.78%), and nonformation (14.62%). Compared with adolescents with an early formation pattern of handwashing habits, those with other patterns exhibited lower odds ratios (ORs) of handwashing during COVID-19; these ORs were 0.67 (95% confidence interval [CI], 0.49-0.85), 0.60 (95% CI, 0.44-0.77), 0.29 (95% CI, 0.08-0.49), and 0.21 (95% CI, 0.01-0.40) for those with delayed formation, gradual formation, inconsistent formation, and nonformation patterns, respectively. Moreover, relative to that of adolescents with the early formation pattern, mask-wearing was less common among adolescents with gradual formation, inconsistent formation, and nonformation patterns, with ORs of 0.54 (95% CI, 0.16-0.92), 0.50 (95% CI, 0.03-0.96), and 0.26 (95% CI, 0.00-0.65), respectively., Conclusions: The early formation of hygienic habits is associated with higher adherence to pandemic preventive practices among adolescents. Our findings suggest that interventions to promote hygienic behaviors can start as early as age 3 through the introduction of healthy habits such as handwashing., (Copyright © 2022 Academic Pediatric Association. Published by Elsevier Inc. All rights reserved.)

Background: Developmental and epileptic encephalopathy 9 (DEE9, MIM #300088) is an early onset seizure disorder associated with cognitive impairment and behavioral disturbances. It is caused by mutation in protocadherin 19 with an unusual X-linked inheritance selectively involving heterozygous females or mosaic hemizygous males, while hemizygous males are unaffected. Cellular interference was the postulated mechanism underlying the unusual inheritance pattern., Case Report: We report a Chinese girl who presented with severe treatment refractory seizures at 26 months of age and was found heterozygous for a novel likely pathogenic missense variant NM_001184880.2:c.488T>A p.(Val163Glu) in PCDH19. Her younger sister, who was also heterozygous for the variant, was asymptomatic with normal development at the time of reporting at 37 months of age. X-chromosome inactivation study by androgen receptor gene methylation assay in DNA from peripheral leukocytes was performed which demonstrated somewhat skewed X-chromosome inactivation in the proband and extremely skewed X-chromosome inactivation in the asymptomatic younger sibling., Conclusion: PCDH19-related seizure disorder has incomplete penetrance and variable expressivity. Further studies are required to determine the potential role of X-chromosome inactivation on the phenotypic variability and patient outcomes. Liberal referral for PCDH19 testing among female patients with early-onset seizures should be considered to enhance case detection., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Objective: The optimal measuring timing of serum/plasma Cystatin C (CysC) for early detection of contrast-induced acute kidney injury (CIAKI) remains un-studied. We elucidated further on this issue., Methods: We searched PubMed, MEDLINE, and Embase from inception until March 2018 for studies evaluating diagnostic accuracy of CysC for detecting CIAKI in patients exposed to contrast agents during diagnostic examinations or cardiac/peripheral catheterizations., Results: A total of 10 relevant studies, comprising 2554 patients, were included and divided into the <24 -h and 24 -h groups based on CysC measuring timing (i.e., hours after contrast agent exposure). Compared with creatinine, pooled diagnostic odds ratio of CysC for detecting CIAKI of the <24 -h and 24 -h groups was 7.59 (95 % confidence interval [CI]: 1.31-44.08) and 53.81 (95 % CI: 13.57-213.26). Pooled sensitivity of the <24 -h and 24 -h groups was 0.81 and 0.88. Pooled specificity of the <24 -h and 24 -h groups was 0.64 and 0.88, respectively. Area under the hierarchical summary receiver operating characteristic curve of the <24 -h and 24 -h groups was 0.75 and 0.93., Conclusions: Measuring CysC at 24 h after contrast agent exposure shows higher diagnostic accuracy for early detection of CIAKI than measuring CysC at <24 h after contrast agent exposure., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Background: Ventricular tachycardia (VT), which is related to haemodynamic and electrophysiological alterations, is an important complication in repaired tetralogy of Fallot (rTOF) patients. We defined the role of implantable cardioverter defibrillator (ICD) therapy after pulmonary valve replacement (PVR) and the implications of coexisting long QT gene mutations/polymorphisms., Methods: From 2003 to 2016, rTOF patients with VT who received ICD implantation were enrolled. rTOF patients without VT served as a control group. We performed long QT gene mutation analysis through the direct sequencing method., Results: In total, 12 (male/female, 8/4) patients with VT received ICD implantation. The mean age at TOF repair and ICD implantation was 4.4±4.5years and 27.1±11.5years, respectively. All but one patient received PVR for severe pulmonary regurgitation or repair for a residual ventricular septal defect. After 5.4±4.0years of follow-up, six patients (50%) had at least one episode of appropriate shock: two had received PVR with intraoperative arrhythmia ablation and two had received PVR only. Compared with 121 patients without VT, the ICD patients had a higher frequency of long QT gene mutation/polymorphisms (10/12 vs. 54/121, p=0.014), especially in the hERG and SCN5A genes. The mechanism of VT in rTOF was discussed thoroughly., Conclusion: In rTOF patients who received ICD as secondary prevention, the appropriate shock rate remained high despite PVR and intraoperative arrhythmia ablation. Coexisting long QT gene mutations/polymorphisms may be associated with ventricular arrhythmia., (Copyright © 2017. Published by Elsevier B.V.)

Purpose: Peers and dates are common targets of adolescent violence. Prevention programs typically address either peer violence (PV) or dating violence (DV) but not both. However, if PV and DV share predictors, prevention strategies could target both behaviors, yielding economic and time efficiencies. Longitudinal data were examined to determine the extent to which physical PV and DV shared predictors. Guided by social learning and social control theories, both risk and protective factors were examined at multiple levels of the social ecology., Methods: Adolescents in the eighth through 10th grades in three North Carolina counties completed self-administered questionnaires in school in the fall 2003 (Wave 1) and again in spring 2004 (Wave 2) (n = 4,227). The sample was 48% male; 55% white, 33% black, and 12% of other race/ethnicity. A generalized estimating equations approach used adjusted standard errors to account for the correlation between the two violence outcomes., Results: For both boys and girls, anger, family conflict, and having models of deviant behavior in the school were shared risk factors, and holding prosocial beliefs was a shared protective factor. For girls, anxiety and having models of deviant behavior in the neighborhood were additional shared risk factors. For boys, heavy alcohol use was an additional shared risk factor and parental monitoring was an additional shared protective factor., Conclusions: Findings can inform the development of comprehensive cross-cutting prevention strategies at multiple levels of the social ecology designed to prevent both types of violence., (Copyright © 2015 Society for Adolescent Health and Medicine. All rights reserved.)

Purpose: One suggested approach to preventing adolescent dating violence is to prevent behavioral precursors to dating violence, such as bullying. However, no longitudinal study has examined bullying as a behavioral precursor to dating violence. In this study, longitudinal data were used to examine (1) whether direct and indirect bullying perpetration in the sixth grade predicted the onset of physical dating violence perpetration by the eighth grade and (2) whether the associations varied by sex and race/ethnicity of the adolescent., Methods: Data were collected in school from sixth graders in three primarily rural counties and then again when students were in the eighth grade. Analyses were conducted with 1,154 adolescents who had not perpetrated dating violence at the sixth-grade assessment. The sample was 47% male, 29% black, and 10% of another race/ethnicity than black or white., Results: Direct bullying, defined as hitting, slapping, or picking on another kid in the sixth grade, predicted the onset of physical dating violence perpetration by the eighth grade, controlling for indirect bullying and potential confounders. Although indirect bullying, defined as spreading false rumors and excluding students from friendship groups, was associated with the onset of physical dating violence perpetration in bivariate analyses, it did not predict the onset of physical dating violence when controlling for direct bullying. None of the associations examined varied by sex or race/ethnicity of the adolescents., Conclusions: Our findings suggest that efforts targeted at preventing direct bullying may also prevent the onset of physical dating violence., (Copyright © 2014 Society for Adolescent Health and Medicine. All rights reserved.)

Purpose: It is widely held that being victimized by a dating partner during adolescence has negative consequences, yet few longitudinal studies have examined those consequences. This longitudinal study examined the effects of psychological and physical (including sexual) dating abuse victimization on internalizing symptoms, substance use, academic aspirations and grades, and relationships with friends and family., Methods: This four-wave longitudinal study (N = 3,328), conducted in two rural North Carolina counties, spanned grades 8 to 12. Random coefficient analyses were used to examine prospective lagged effects of each type of dating abuse on each outcome and to examine sex and grade as moderators of lagged effects., Results: Consequences varied by type of dating abuse experienced and sex. For both boys and girls, psychological victimization predicted increased alcohol use and physical victimization predicted increased cigarette use. For girls, physical victimization predicted increased marijuana use, and psychological victimization predicted increased internalizing symptoms; the latter effect was only marginally significant for boys. Physical victimization marginally predicted decreases in the number of close friends for boys. Neither type of victimization predicted increased family conflict or decreased academic aspirations or grades, nor was there evidence that consequences varied by grade., Conclusions: Although causation cannot be concluded with longitudinal designs, our findings suggest that being victimized by a dating partner may result in detrimental consequences for adolescents. The findings demonstrate the importance of identifying and implementing evidence-based interventions for preventing dating abuse, including efforts to prevent psychological abuse specifically., (Copyright © 2013 Society for Adolescent Health and Medicine. Published by Elsevier Inc. All rights reserved.)

The objective of this study is to evaluate the specificity and sensitivity of antinucleosome, anti-C1q, and anti-dsDNA antibodies for detecting concurrent disease activity in systemic lupus erythematosus (SLE). Consecutive patients were recruited for serological testing of anti-dsDNA, IgG-antinucleosome, IgG-anti-C1q, and complement levels. SLE disease activity was assessed by SLEDAI and physician's global assessment (PGA). The levels of these antibodies, complements, and disease activity scores were correlated. The specificity and sensitivity of these antibodies in detecting SLE activity was determined. We recruited 245 SLE patients (95% women, age 40.6 ± 12.2 years). The prevalence of positive anti-dsDNA, antinucleosome and anti-C1q antibodies was 55%, 44%, and 21%, respectively. All 3 antibodies correlated significantly with SLEDAI and PGA scores but were correlated inversely with complement levels (P < 0.001 in all). Titers of anti-dsDNA and anti-C1q, but not antinucleosome, correlated significantly with the renal SLEDAI score. The sensitivity of anti-dsDNA, antinucleosome, and anti-C1q for detecting the presence of active renal disease was 75%, 47%, and 53%, respectively. Anti-C1q had the highest specificity for active lupus renal disease (84%) followed by antinucleosome (57%) and the anti-dsDNA antibody (49%). The negative predictive value (NPV) of a negative anti-dsDNA and anti-C1q for active renal disease was 91%. For concurrent extrarenal SLE activity, the specificity was also highest with anti-C1q (83%). We conclude that antinucleosome does not perform better than anti-dsDNA for detecting concurrent SLE activity. The anti-C1q antibody, however, is more specific than anti-dsDNA for both active renal and extrarenal lupus. The absence of both anti-dsDNA and anti-C1q has a high NPV for renal activity., (Copyright © 2010 Mosby, Inc. All rights reserved.)