Search

The study presents a comprehensive examination of changes in soil microbial functional diversity (hereafter called microbial activity) following the implementation of Nature-Based Solutions (NBS) in urban areas. Utilizing the Biolog® EcoPlates™ technique, the study explored variations in microbial diversity in urban soil under NBSs implementation across timespan of two years. Significant differences in microbial activity were observed between control location and those with NBS implementations, with seasonal variations playing a crucial role. NBS positively impacted soil microbial activity especially at two locations: infiltration basin and wild flower meadow showing the most substantial increase after NBS implementation. The study links rainfall levels to microbial functional diversity, highlighting the influence of climatic conditions on soil microbiome. The research investigates also the utilization of different carbon sources by soil microorganisms, shedding light on the specificity of substrate utilization across seasons and locations. The results demonstrate that NBSs implementations lead to changes in substrate utilization patterns, emphasizing the positive influence of NBS on soil microbial communities. Likewise, biodiversity indices, such as Shannon-Weaver diversity (H'), Shannon Evenness Index (E), and substrate richness index (S), exhibit significant variations in response to NBS. Notably, NBS implementation positively impacted H' and E indexes, especially in infiltration basin and wild flower meadow, underlining the benefits of NBS for enhancing microbial diversity. The obtained results demonstrated valuable insight into the dynamic interactions between NBS implementation and soil microbial activity. The findings underscore the potential of NBS to positively influence soil microbial diversity in urban environments, contributing to urban sustainability and soil health. The study emphasizes the importance of monitoring soil microbial activity to assess the effectiveness of NBS interventions and guides sustainable urban development practices., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The diversity of fungal strains is influenced by genetic and environmental factors, growth conditions and mycelium age, and the spectral features of fungal mycelia are associated with their biochemical, physiological, and structural traits. This study investigates whether intraspecific differences can be detected in two closely related entomopathogenic species, namely Cordyceps farinosa and Cordyceps fumosorosea, using ultraviolet A to shortwave infrared (UVA-SWIR) reflectance spectra. Phylogenetic analysis of all strains revealed a high degree of uniformity among the populations of both species. The characteristics resulting from variation in the species, as well as those resulting from the age of the cultures were determined. We cultured fungi on PDA medium and measured the reflectance of mycelia in the 350-2500 nm range after 10 and 17 days. We subjected the measurements to quadratic discriminant analysis (QDA) to identify the minimum number of bands containing meaningful information. We found that when the age of the fungal culture was known, species represented by a group of different strains could be distinguished with no more than 3-4 wavelengths, compared to 7-8 wavelengths when the age of the culture was unknown. At least 6-8 bands were required to distinguish cultures of a known species among different age groups. Distinguishing all strains within a species was more demanding: at least 10 bands were required for C. fumosorosea and 21 bands for C. farinosa. In conclusion, fungal differentiation using point reflectance spectroscopy gives reliable results when intraspecific and age variations are taken into account., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

The study assessed the occurrence of legally-monitored heavy metals and unmonitored antimicrobials in sludge from small, medium, large and very large municipal wastewater treatment plants (WWTPs), and the predicted environmental risk and risk of resistance selection associated with sludge administration to soil. The temporal variations of the studied compounds in sludge and associated risks to soil were determined by sampling over a year. Although the highest concentrations of heavy metals were noted in sludge from the largest WWTP, i.e. from 1.50 mg/kg (mean 1.61 mg/kg) for Cd to 2188 mg/kg (mean 1332 mg/kg) for Zn, the obtained values only reached a few percent of the legal limits. The same WWTP also demonstrated lower concentrations of antimicrobials compared to the smaller ones. The highest concentrations of antimicrobials, ranging from 24.04 μg/kg for trimethoprim to 900.24 μg/kg for tetracycline, were found in the small and medium WWTPs. However, due to lack of regulations at the national and EU levels, the results cannot be compared with legal limits. Principal Component Analysis (PCA), cluster and heatmap analysis separated samples according to WWTP size. Small WWTP demonstrated correlation with antimicrobials (tetracycline, trimethoprim, clindamycin, ciprofloxacin and ofloxacin), while the large and very large WWTP revealed correlations with heavy metals (Cu and Cr). The obtained environmental risk quotients confirmed that the heavy metals did not present a threat, measured either as individual risk quotients (RQ env ), cumulative risk (RQ cumulative ) or risk of mixture of heavy metals (RQ mix-metals ). In the case of antimicrobials, only tetracycline demonstrated moderate RQ env , RQ cumulative and RQ mix-antimicrobials in the small WWTP sludge, with values of 0.1 to 1. Our findings highlight the importance of monitoring sewage sludge before soil application, especially from small WWTPs, to reduce the potential environmental impact of antimicrobials. They also confirm our previous data regarding the environmental risk associated with various toxic compounds, including emerging contaminants, in the sludge from small WWTPs., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

MCPA (2-methyl-4-chlorophenoxyacetic acid) contamination is an emerging problem, especially in water reservoirs. The early removal of MCPA residues from soil can prevent its spread to untreated areas. It has been found that the growth of cucurbits and the addition of selected plant secondary metabolites (PSMs) can stimulate MCPA removal from soil. However, the effect of these treatments on soil microbial activity remains poorly studied. Hence, the aim of this research was to evaluate the influence of zucchini (C. pepo cv Atena Polka) and its characteristic PSM: syringic acid (SA) on the functional diversity of soil microorganisms in MCPA-contaminated soil using Biolog® EcoPlates™. It also examines soil physicochemical properties and the growth parameters of zucchini. Microbial activity was enhanced by both zucchini cultivation and SA. All unplanted variants showed significantly lower microbial activity (average well color development, AWCD, ranging from 0.35 to 0.51) than the planted ones (AWCD ranging from 0.77 to 1.16). SA also stimulated microbial activity in the soil: a positive effect was observed from the beginning of the experiment in the unplanted variants, but over a longer time span in the planted variants. SA ameliorated the toxic effect of MCPA on the studied plants, especially in terms of photosynthetic pigment production: the MCPA+SA group demonstrated significantly increased chlorophyll content (401 ± 4.83 μg/g), compared to the MCPA group without SA (338 ± 50.1 μg/g). Our findings demonstrated that zucchini and the amendment of soils with SA, the characteristic PSM of cucurbits, can shape functional diversity in MCPA-contaminated soil. The changes of soil properties caused by the application of both compounds can trigger changes in functional diversity. Hence, both SA and MCPA exert indirect and direct effects on soil microbial activity., Competing Interests: Declaration of competing interest The authors declare no financial interests/personal relationships which may be considered as potential competing interests., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Despite extensive use of primary aromatic amines (AAs) in consumer products, little is known about their occurrence in the environment. In this study, we investigated the occurrence of 14 AAs and nicotine in 75 sediment samples collected from seven estuarine and freshwater ecosystems in the Unites States. Additionally, risk quotients (RQs) were calculated to assess potential risks of these chemicals to aquatic organisms. Of the 14 AAs analyzed, seven of them were found in sediments. The sum concentrations of seven AAs in sediments were in the range of 10.2 to 1810 ng/g, dry wt (mean: 388 ng/g). Aniline was the most abundant compound, accounting for, on average, 53 % of the total concentrations. Nicotine was found in sediments at a concentration range of

The potential use of growth substrates prepared with an admixture of 10% to 75% Hudson River sediments was evaluated by analysis of changes in microbial activity (measured using Biolog Ecoplates) and molecular markers (presence of degradative tceA1 and bphA genes) as well as potential risks toward humans and the environment (health and environmental risk quotients). The highest microbial activity was found in growth substrate with 25% Hudson River sediments compared to unamended control soil. Significant differences were observed between samples amended with lower (0-10%) and higher (25-75%) proportion of sediment. Microbial activity increased with the proportion of sediment amendment (≥25% sediment); however, this increase in microbial activity was not affected by increasing pollutant concentrations (PCBs, Pb, Cr Ni and Zn) nor decreasing TOC content. The growth substrate amended with Hudson River sediments demonstrated a potential for PCB degradation, as evidenced by the presence of tceA1 and bphA genes responsible, respectively, for reductive dehalogenation and oxidation of a range of aromatic organic compounds including PCBs. An assessment of risk quotients showed that the growth substrates containing lower doses of Hudson River sediments (10-50%) meet the international requirements for use in agriculture/horticulture for the production of non-food crops. Nevertheless, due to the elevated content of some toxic metals and PCBs, the growth substrate prepared with the highest proportion of sediments (75%) was not suitable for agricultural/horticultural use., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

The need to dispose of dredged sediments and development of appropriate technology for their safe utilization has become a growing problem in recent years. It has been proposed that dredged, fresh sediments can be utilized in agriculture or environment; however there is also growing interest in the use of thermally-treated sediments. Hence, the aim of this study was threefold: 1) to evaluate the effect of two incineration temperatures (300 °C and 600 °C) on the chemical and ecotoxicological properties of sediment; 2) select the appropriate treatment for further phytoremediation experiments with zucchini; and 3) assess the impact of sediment admixture on the physico-chemical parameters of soil, based on the responses of Aliivibrio fischeri and growth of zucchini (Cucurbita pepo L. cv 'Black Beauty'). A range of chemical (inductively-coupled plasma optical emission spectrophotometry for macro- and trace elements; gas chromatography for polychlorinated biphenyls (PCBs)), ecotoxicological (Microtox assay), and plant morphology (biomass measurement) as well as physiological analyses (spectrophotometry for chlorophyll) were applied. River sediments incinerated at 600 °C resulted in better chemical and ecotoxicological properties than incinerated at 300 °C or no incinerated. Incineration at 600 °C removed PCBs from sediment. In culture experiments conducted with zucchini, sediment treated at 300 °C demonstrated a 51-81% reduction in PCB concentrations compared to untreated sediment. After four weeks of growth, the raw sediment showed a significant increase in K, Fe, Cr, Pb, Zn concentrations, whereas the thermally-processed sediment showed a decrease in Ca, Na, P, Cd, Cu, Ni, and Zn concentrations. Both the fresh and thermally-treated sediment types influenced plant growth positively: they demonstrated higher biomass production than plants grown in control soil; however, plants grown on soil with thermally-processed sediment demonstrated lower biomass than those grown in raw sediment. Chlorophyll content was affected negatively by admixtures of soil with treated or untreated sediment, while a lower chlorophyll a/b ratio was observed in plants grown on an admixture of thermally-treated sediment with soil. Our findings suggest that the use of sediments as a growth medium component may be a promising way for their utilization and transformation from waste material to a valuable resource enhancing the benefits to the environment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.)

Sewage sludge production is increasing, as is the importance of its safe and sustainable disposal. The study examine the impact of fertilizing poor quality soil with sewage sludge from three sizes of wastewater treatment plants (WTP) (small, medium and large) located in Central Poland, applied in two doses (3 and 9tons per hectare), on biomass yield and selected biochemical parameters in willow plants. The WTPs were selected based on the size criterion given in Polish regulations. Three variables were analyzed in the leaves and roots of willow cuttings after twenty weeks of cultivation: the degree of oxidative reaction, assessed as thiobarbituric-acid-reactive substances (TBARS) content; catalase activity (CAT, EC 1.11.1.6), reflecting the efficiency of the antioxidative response; and changes in the concentration of proline, one of the major cellular osmoprotectants. For plants growing in poor-quality soil, sewage sludge administration results in a large increase of willow biomass by creating good conditions for plant growth associated with protection against oxidative damage, efficient functioning of the antioxidant system and maintenance of the osmotic balance between the soil environment and plant roots. The greatest increase was 204% of control in the case of 9tha -1 sludge from the medium WTP. Biomass production correlated with the applied sludge dose. After sewage sludge application lipid peroxides were reduced in leaves (81%) and roots (69%), CAT activity was enhanced (to 855% in leaves) and proline level was increased in root tissues. The application of sewage sludge originating from small WTPs offers the best conditions than larger WTPs: sludge from large WTPs may cause oxidative damage and exceed the possibilities of plants to counteract stress factors, especially when used in high doses., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Fusarium proliferatum is a world-wide occurring fungal pathogen affecting several crops included garlic bulbs. In Spain, this is the most frequent pathogenic fungus associated with garlic rot during storage. Moreover, F. proliferatum is an important mycotoxigenic species, producing a broad range of toxins, which may pose a risk for food safety. The aim of this study is to assess the intraspecific variability of the garlic pathogen in Spain implied by analyses of translation elongation factor (tef-1α) and FUM1 gene sequences as well as the differences in growth rates. Phylogenetic characterization has been complemented with the characterization of mating type alleles as well as the species potential as a toxin producer. Phylogenetic trees based on the sequence of the translation elongation factor and FUM1 genes from seventy nine isolates from garlic revealed a considerable intraspecific variability as well as high level of diversity in growth speed. Based on the MAT alleles amplified by PCR, F. proliferatum isolates were separated into different groups on both trees. All isolates collected from garlic in Spain proved to be fumonisin B 1 , B 2 , and B 3 producers. Quantitative analyses of fumonisins, beauvericin and moniliformin (common secondary metabolites of F. proliferatum) showed no correlation with phylogenetic analysis neither mycelial growth. This pathogen presents a high intraspecific variability within the same geographical region and host, which is necessary to be considered in the management of the disease., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

The aim of the study was to determine the impact of sewage sludge from three wastewater treatment plants of different sizes (small, medium and large) applied in two doses (3 and 9 tons per hectare) on soil properties, determined as the content of organic carbon and humus fractions, bacterial abundance, phytotoxicity and PCDD/PCDF TEQ concentrations. The study also evaluated the impact of this sewage sludge on the biometric and physiological parameters and detoxification reaction of willow (Salix sp.) as a typical crop used for the remediation of soil following sludge application. The cultivation of willow on soil treated with sludge was found to result in a gradual increase of humus fractions, total organic carbon content and bacterial abundance as well as soil properties measured using Lepidium sativum. However, it also produced an initial increase of soil phytotoxicity, indicated by Sinapis alba and Sorghum sacharatum, and PCDD/PCDF Toxic Equivalent (TEQ) concentrations, which then fell during the course of the experiment, particularly in areas planted by willow. Although the soil phytotoxicity and PCDD/PCDF TEQ content of the sewage sludge-amended soil initially increased, sludge application was found to have a positive influence on willow, probably due to its high nutrient and carbon content. The obtained results reveal increases in willow biomass, average leaf surface area and leaf length as well as chlorophyll a+b content. Moreover, a strong decline was found in the activity of the detoxifying enzyme glutathione S-transferase (GSTs), a multifunctional enzyme involved in the metabolism of xenobiotics in plants, again demonstrating the used sludge had a positive influence on willow performance., (Copyright © 2017 Elsevier B.V. All rights reserved.)