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The chemical components in the natural aquatic environment have the potential to be involved in phototransformation of microplastics (MPs). Little information is available regarding the mediation effects of artificially introduced chemicals on MP phototransformation, especially those used in aquaculture water that are vulnerable to human interference. Herein, this study investigated the phototransformation process and mechanism of polypropylene microplastic (PP MPs) in presence of trichloroisocyanuric acid (TCCA) disinfectant with unique properties unlike the conventional inorganic chlorine disinfectants. The results showed that the presence of TCCA inhibited the surface photooxidation of PP MPs. Analysis of PP MP surface and reaction filtrate indicated that the inhibitory effects were likely derived from TCCA derivatives and the weakening in promoting effect of polypropylene microplastic-derived dissolved organic matter (PP-DOM) as photolytic byproducts, with the more important role of free chlorine in initial period and that of other chlorine species (i.e., the adsorbed chloride ions (Cl - ), newly formed carbon-chlorine (CCl) bonds, chlorinated cyanurates, and chlorinated products) in middle and later period. The study highlights for the first time the important role of chlorine species derived from TCCA in phototransformation process of co-existed PP MPs and proposes a previously unrecognized phototransformation pathway, which will provide a new understanding and knowledge for the environmental behavior of MPs in aquaculture environment., (Copyright © 2024. Published by Elsevier B.V.)

The global escalation and intensification of cyanobacterial blooms require powerful algaecides. This study investigated the algicidal efficacy and mechanism of EA-1 against Oscillatoria. Bacteria EA-1, identified as Enterobacter, was isolated with high algicidal activity against harmful cyanobacteria. Results showed that a complete removal of Oscillatoria was observed within 3 days with the initial Chl-a concentration of 1.74 mg/L. Physiological responses of Oscillatoria revealed that EA-1 induced severe lipid peroxidation and the ultimate decline of antioxidant enzyme activities. Moreover, the contents for both intracellular protein and carbohydrate of each algae cell increased first and then decreased. Scanning electron microscope (SEM) and transmission electron microscopy (TEM) analysis clarified that the possible process of Oscillatoria lysis included the breach of cross wall, followed by the disruption of photosynthetic membrane and incipient nucleus, and the ultimate outflow of inclusion. Confocal laser scanning microscopy (CLSM) analysis illustrated the degradation process of incipient nucleus in Oscillatoria., (Copyright © 2021 Elsevier Inc. All rights reserved.)

Microplastics (MPs) have caused great concern worldwide recently due to their ubiquity in the aquatic environment. The current knowledge on the occurrence of MPs in aquaculture fish ponds in a typical estuary system remains meagre. This study investigated the abundance and characteristics (shape, color, size and composition) of MPs in aquaculture water and pond influents in the Pearl River Estuary of Guangzhou, China, using an improved separation method. The bulk sampling and improved separation method by the combination of ethanol and polyaluminum chloride (PAC) significantly increased the MP separation efficiency, especially for particles with size less than <333 μm. The investigation results showed that MPs were detected in all water samples of fish ponds at two experimental stations with abundances of 10.3-60.5 particles/L (S1) and 33.0-87.5 particles/L (S2), respectively. Moreover, the average abundance of MPs in aquaculture water (42.1 particles/L) exhibited higher value than that in pond influents (32.1 particles/L). Most of MPs were colored and fibrous in appearance. MPs with the size range of <1000 μm (56.3-87.7%) prevailed in aquaculture water. MPs with size <333 μm that usually ignored in most studies were detected with percentage of 43.7% at S1station and 33.2% at S2 station, respectively. The small-sized MPs (<100 μm) in aquaculture water (23.7% at S1 station and 14.6% at S2 station) were more abundant than those in pond influents (7.2% at S1 station and 2.5% at S2 station). The main composition of MPs was polypropylene (PP) and polyethylene (PE). These findings indicated a high level of MP pollution in aquaculture fish ponds. The MPs originated from the Pearl River Estuary were accumulated in aquaculture fish ponds. This study provides an insight into MP pollution in aquaculture fish ponds at a typical estuarine system and highlights the load of MPs in the pond influents., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

The frequent occurrence of toxin-producing cyanobacteria blooms driven by anthropogenic eutrophication has become a major threat to aquaculture ecosystems worldwide. In this study, the behavior of M. aeruginosa cells during flocs storage period of 6 days was first investigated after pre-oxidation and coagulation of Fe 2+ /PS. Fe 2+ /PS achieved a superior removal efficiency of 90.7% for OD 680 and 90.4% for chl-a. The contents of extracellular MCs in the pre-oxidation and coagulation system were significantly (P < 0.05) lower than those in the control. A significant (P < 0.05) difference in intracellular protein between the control and the coagulated systems was observed. Three-dimensional fluorescence excitation emission matrix (EEM) was employed to investigate the variations in extracellular organic matter (EOM) during flocs storage. The results indicated the presence of four peaks, representing protein-like substances, intermediate dissolved microbial metabolites, fulvic and humic-like compounds in the Fe 2+ /PS process. And the intensities of four peaks were all decreased in the Fe 2+ /PS system compared to those in the control. A low level of accumulated residual Fe of 0.28 mg/L was observed without posing potential environmental risk. The results showed that the M. aeruginosa cells were under stressful conditions after 3-d storage due to the decomposition of extracellular polymeric substances (EPSs) and the insufficient supply of nutrients. However, SEM results indicated that no significant alteration in cell morphology was observed. Therefore, with high removal of M. aeruginosa, low MCs concentrations, and trivial cell damage, the Fe 2+ /PS preoxidation-coagulation was proved to be an environmental-friendly method for cyanobacteria removal without yielding serious secondary pollution. This work will contribute to better understanding and managing the cyanobacteria-laden aquaculture water after pre-oxidation and coagulation., Competing Interests: Declaration of competing interest There are no interests to declare., (Copyright © 2020. Published by Elsevier Inc.)

The HER2 pathway plays a pivotal role in cell proliferation and differentiation, while the receptor overexpression caused by amplification of HER2 gene is associated with the growth of several tumors. Previously published clinical trials have demonstrated that antibody-conjugated drugs (ADCs) remarkably improved clinical effects compared with antibodies alone for the same target. In order to provide more effective drugs, we developed Disitamab vedotin based on ADC. The antibody part was a humanized monoclonal antibody targeting HER2, the small molecule toxin was monomethyl auristatin E (MMAE), a synthetic antineoplastic agent. A protease cleavable linker covalently attached MMAE to the antibody. In this study, we characterized the toxicity profile of Disitamab vedotin through single- and repeat-dose toxicity studies in monkeys. The toxicities of small molecules and naked antibody (Disitamab) were also assessed in these studies. Monkeys were well tolerated with Disitamab vedotin at doses of 6 mg/kg, while equivalent MMAEs resulted in severe myelosuppression. This finding proves that ADCs improve the therapeutic effect. In addition, the safety profiles of Disitamab vedotin and MMAE were similar and consistent with the activation mechanism of MMAE. Toxicology finding included bone marrow/hematology toxicity and lymphoid organ toxicity, while no significant toxicity was observed in animals treated with naked antibody. These side effects were found to be consistent with data acquired from clinical phase I/II patients treated with Disitamab vedotin., Competing Interests: Declaration of Competing Interest The authors declare that they have no conflict of interest., (Copyright © 2019. Published by Elsevier B.V.)

Introduction: Planning for the future is important for adolescents' development and academic adaptation. Most prior research has focused primarily on the effects of future planning on adolescents' academic achievement, and there is a dearth of literature examining the effects of adolescents' academic achievement on their future planning. The current study examined the longitudinal reciprocal relations between adolescents' future planning and academic achievement in China., Methods: The study sample consisted of 775 Chinese junior high school students (391 boys, 384 girls; M age  = 13.38 years, SD = 0.89). Future planning and academic achievement were measured at three time points that were equally spaced by a 6-month lag. Path analyses with cross-lagged effects were used to examine the longitudinal reciprocal relations between adolescents' future planning and academic achievement., Results: The associations between future educational planning and academic achievement were reciprocal. Future occupational commitment predicted adolescents' academic achievement, and academic achievement predicted adolescents' affects concerning future occupation. However, there was no significant association between future occupational exploration and academic achievement. Moreover, these patterns of longitudinal relations applied equally to boys and girls, and younger and older adolescents., Conclusions: The reciprocal relations between future planning processes and academic achievement differed across domains (e.g., educational and occupational domains). These findings suggest that future planning may be a protective factor for improving adolescents' academic achievement, and high academic achievement may also be beneficial for Chinese adolescents' future planning., (Copyright © 2019 The Foundation for Professionals in Services for Adolescents. Published by Elsevier Ltd. All rights reserved.)

Enrichment of calcium phosphate (CaP) bone substitutes with poly(lactic-co-glycolic acid) (PLGA) microspheres to create porosity overcomes the problem of poor CaP degradation. The degradation of CaP-PLGA composites can be customized by changing the physical and chemical properties of PLGA and/or CaP. However, the effect of the size of dense (solid rather than hollow) PLGA microspheres in CaP has not previously been described. The present study aimed at determining the effect of different dense (i.e. solid) PLGA microsphere sizes (small (S) ~20μm vs. large (L) ~130μm) and of CaP composition (CaP with either anhydrous dicalcium phosphate (DCP) or calcium sulphate dihydrate (CSD)) on CaP scaffold biodegradability and subsequent bone in-growth. To this end mandibular defects in minipigs were filled with pre-set CaP-PLGA implants, with autologous bone being used as a control. After 4weeks the autologous bone group outperformed all CaP-PLGA groups in terms of the amount of bone present at the defect site. On the other hand, at 12weeks substantial bone formation was observed for all CaP-PLGA groups (ranging from 47±25% to 62±15%), showing equal amounts of bone compared with the autologous bone group (82±9%), except for CaP with DCP and large PLGA microspheres (47±25%). It was concluded that in the current study design the difference in PLGA microsphere size and CaP composition led to similar results with respect to scaffold degradation and subsequent bone in-growth. Further, after 12weeks all CaP-PLGA composites proved to be effective for bone substitution., (Copyright © 2013 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.)