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Drugs are chemical compounds used to treat and improve organic dysfunctions caused by diseases. These include analgesics, antibiotics, antidepressants, and antineoplastics. They can enter aquatic environments through wastewater streams, where their physico-chemical properties allow metabolites to distribute and accumulate. Current climate change and associated extreme weather events may significantly impact these substances' toxicity and aquatic organisms' sensitivity. Among the chemicals present in aquatic environments is the non-steroidal anti-inflammatory drug diclofenac (DIC), which the EU monitors due to its concentration levels. This study investigated the influence of temperature (control at 17 °C vs. 21 °C) on the effects of DIC (0 μg/L vs. 1 μg/L) in the mussel species Mytilus galloprovincialis. Significant results were observed between 17 and 21 °C. Organisms exposed to the higher temperature showed a decrease in several parameters, including metabolic capacity and detoxification, particularly with prolonged exposure. However, in some parameters, after 21 days, the M. galloprovincialis showed no differences from the control, indicating adaptation to the stress. The results of this study confirm that DIC concentrations in the environment, particularly when combined with increased temperatures, can produce oxidative stress and adversely affect M. galloprovincialis biochemical and physiological performance. This study also validates this species as a bioindicator for assessing environmental contamination with DIC. Beyond its direct impact on aquatic organisms, the presence of pharmaceuticals like DIC in the environment highlights the interconnectedness of human, animal, and ecosystem health, underscoring the One Health approach to understanding and mitigating environmental pollution., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

In aquatic ecosystems, the presence of pharmaceuticals, particularly caffeine (CAF), has been linked to wastewater discharge, hospital waste, and the disposal of expired pharmaceutical products containing CAF. Additionally, rising temperatures due to climate change are anticipated in aquatic environments. This study aimed to assess the toxicity of various CAF concentrations under current (17 °C) and projected (21 °C) temperature conditions, using the mussel Mytilus galloprovincialis as a bioindicator species. Subcellular impacts were evaluated following 28 days of exposure to four CAF concentrations (0.5; 1.0; 5.0; 10.0 μg/L) at the control temperature (17 °C). Only effects at an environmentally relevant CAF concentration (5.0 μg/L) were assessed at the highest temperature (21 °C). The overall biochemical response of mussels was evaluated using non-metric Multidimensional Scaling (MDS) and the Integrated Biomarker Response (IBR) index, while the Independent Action (IA) model was used to compare observed and predicted responses. Results showed that at 17 °C, increased CAF concentrations were associated with higher metabolism and biotransformation capacity, accompanied by cellular damage at the highest concentration. Conversely, under warming conditions (21 °C), the induction of antioxidant enzymes was observed, although insufficient to prevent cellular damage compared to the control temperature. Regarding neurotoxicity, at 17 °C, the activity of the acetylcholinesterase enzyme was inhibited up to 5.0 μg/L; however, at 10.0 μg/L, activity increased, possibly due to CAF competition for adenosine receptors. The IA model identified a synergistic response for most parameters when CAF and warming acted together, aligning with observed results, albeit with slightly lower magnitudes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)

Background: In patients with cirrhosis, infections significantly increase the risk of short and long-term mortality. During infection, the levels of procalcitonin increase, but it has not yet been clarified its prognostic value in subjects with cirrhosis. Therefore, the aim of this study was to evaluate the prognostic role of procalcitonin in patients with liver cirrhosis hospitalized for acute infection, and to compare it with other markers of infection., Patients: We included 279 patients hospitalized because of infection, 133 with liver cirrhosis. At admission the levels of the main biochemical parameters of infection, i.e. leukocytes, procalcitonin, C reactive protein and lactate, were considered., Results: The duration of hospitalization and antibiotic therapy were longer in patients with cirrhosis, while no difference was observed for mortality. In both groups, a correlation with the duration of hospitalization and antibiotic therapy was observed for high levels of procalcitonin. In the cirrhotic population, in particular, higher procalcitonin values were associated with an increase in the length of hospitalization and antibiotic therapy, suggesting an even greater predictive value for those patients. High levels of leucocytes and lactate were positively associated with the duration of hospitalization, but not with the duration of antibiotic therapy. For mortality, the strongest correlation was found for high serum lactate levels, regardless of the presence of cirrhosis., Conclusion: In patients with cirrhosis and acute infection, the value of procalcitonin at admission is a good prognostic indicator for the course of hospitalization, and could be useful for guiding the management and treatment of hospitalized patients., Competing Interests: Conflict of Interest The Authors of this manuscript have no conflicts of interest to declare. All co-Authors have seen and agree with the contents of the manuscript and there is no financial interest to report., (Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)

The bisindolic alkaloid caulerpin (CAU) is a bioactive compound isolated from green algae of the genus Caulerpa that are highly invasive in the Mediterranean Sea. On the other side, the purine alkaloid caffeine (CAF) is one of the most globally consumed psychoactive substances and a widespread anthropogenic water pollutant. Both compounds display a large panel of biological properties and are well known to accumulate in the tissues of aquatic organisms and, in certain circumstances, co-occur in the human diet. On this premise, the present study aimed to investigate possible synergistic interactions between CAU and CAF by using the bivalve Mytilus galloprovincialis as a model organism. Mussels were exposed to CAF via medium while they were fed with food enriched with CAU. After treatments, biochemical analysis confirmed the toxic potential of CAF, with increased AChE activity and lipid peroxidation. Also, histopathological alterations were observed in the gills and digestive tubules. The NMR-based metabolomics analysis detected higher levels of free amino acids under CAF treatments. Conversely, the food administration of CAU did not affect the above toxicological biomarkers. In addition, we did not observe any cumulative effect between CAF and CAU toward increased cellular damage and neurotoxicity. On the other hand, a possible action of CAU in decreasing CAF toxicity could be hypothesized based on our results. This hypothesis is supported by the activity of CAU as an agonist of peroxisome proliferator-activated receptors (PPARs). PPARs mediate xenobiotic detoxification via cytochromes P450, which is involved in CAF metabolism. Overall, the results obtained not only rule out any cumulative adverse effects of CAF and CAU but also encourage further research to evaluate the possible use of CAU, a compound easily obtained through the valorization of biomass from invasive species, as a food additive to improve the clearance of xenobiotics., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)

Despite the effects of ocean acidification (OA) on seagrasses have been widely investigated, predictions of seagrass performance under future climates need to consider multiple environmental factors. Here, we performed a mesocosm study to assess the effects of OA on shallow and deep Posidonia oceanica plants. The experiment was run in 2021 and repeated in 2022, a year characterized by a prolonged warm water event, to test how the effects of OA on plants are modulated by thermal stress. The response of P. oceanica to experimental conditions was investigated at different levels of biological organization. Under average seawater temperature, there were no effects of OA in both shallow and deep plants, indicating that P. oceanica is not limited by current inorganic carbon concentration, regardless of light availability. In contrast, under thermal stress, exposure of plants to OA increased lipid peroxidation and decreased photosynthetic performance, with deep plants displaying higher levels of heat stress, as indicated by the over-expression of stress-related genes and the activation of antioxidant systems. In addition, warming reduced plant growth, regardless of seawater CO 2 and light levels, suggesting that thermal stress may play a fundamental role in the future development of seagrass meadows. Our results suggest that OA may exacerbate the negative effects of future warming on seagrasses., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)

Foundation species have been widely documented to provide suitable habitats for other species by ameliorating stressful environmental conditions. Nonetheless, their role in rescuing stress-sensitive species from adverse conditions due to climate change remains often unexplored. Here, we performed a mesocosm experiment to assess whether the seagrass, Posidonia oceanica, through its photosynthetic activity, could mitigate the negative effects of ocean acidification on larval development and growth of the calcifying sea urchin, Paracentrotus lividus. Sea urchin larvae at early and late developmental stages that are generally associated to benthic habitats, were grown in aquaria with or without P. oceanica plants, under ambient or low pH conditions predicted by the end of the century under the worst climate scenario (RCP8.5). The percentage of abnormal larvae and their total body length under different experimental conditions were assessed on early- (i.e., pluteus; 72 h post-fertilization) and final-developmental stages (i.e., echinopluteus; 30 days post-fertilization), respectively. The presence of P. oceanica increased mean daily pH values of ∼0.1 and ∼0.15 units at ambient and low pH conditions, respectively, compared with tanks without plants. When grown at low pH in association with P. oceanica, plutei showed a ∼23 % reduction of malformations and echinoplutei a ∼34 % increase in total body length, respectively, compared with larvae developing in tanks without plants. Our results suggest that P. oceanica, by increasing pH and altering seawater carbonate chemistry through its metabolic activity, could buffer the negative effects of ocean acidification on calcifying organisms and could, thus, represent a tool against climate-driven loss of biodiversity., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

River widening, defined as a lateral expansion of the channel, is a critical process that maintains fluvial ecosystems and is part of the regular functioning of rivers. However, in areas with high population density, channel widening can cause damage during floods. Therefore, for effective flood risk management it is essential to identify river reaches where abrupt channel widening may occur. Despite numerous efforts to predict channel widening, most studies have been limited to single rivers and single flood events, which may not be representative of other conditions. Moreover, a multi-catchment scale approach that covers various settings and flood magnitudes has been lacking. In this study, we fill this gap by compiling a large database comprising 1564 river reaches in several mountain regions in Europe affected by floods of varying magnitudes in the last six decades. By applying a meta-analysis, we aimed to identify the types of floods responsible for more extensive widening, the river reach types where intense widening is more likely to occur, and the hydraulic and morphological variables that explain widening and can aid in predicting widening. Our analysis revealed seven groups of reaches with significantly different responses to floods regarding width ratios (i.e., the ratio between channel width after and before a flood). Among these groups, the river reaches located in the Mediterranean region and affected by extreme floods triggered by short and intense precipitation events showed significantly larger widening than other river reaches in other regions. Additionally, the meta-analysis confirmed valley confinement as a critical morphological variable that controls channel widening but showed that it is not the only controlling factor. We proposed new statistical models to identify river reaches prone to widening, estimate potential channel width after a flood, and compute upper bound width ratios. These findings can inform flood hazard evaluations and the design of mitigation measures., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)

Marine organisms are constantly exposed to multiple stressors including pollutants released into the environment, such as personal care products (PCPs), and climate change-derived factors, namely warming, which are aggravated by anthropogenic drivers and pose increasing pressure on coastal ecosystems. Avobenzone (AVO) is one of the most used ultraviolet (UV) filters in PCPs which have been increasingly used and, thereby, identified in aquatic environments. However, data regarding the influence of warming on the impacts caused by AVO in bivalves is lacking. Mussels are considered good bioindicators thus being often employed in ecotoxicology studies. Hence, the present study aimed to evaluate the toxic effects of an environmentally relevant concentration of AVO (0.5 μg/L) and warming (21 °C), acting alone or in combination, on sperm and adults of the Mediterranean mussel species Mytilus galloprovincialis, through in vitro and in vivo tests, respectively. AVO and warming effects were evaluated by assessing oxidative status, viability, genotoxicity, motility, and kinetics in sperm, together with the quantification of energy content, metabolic capacity, biological defence mechanisms, cellular damage, and neurotoxicity in adults. AVO induced genotoxicity and increased respiration rate in sperm while enhancing the biotransformation enzymes' activity in adults. Exposure to warming led to an increase in respiration rate, ROS overproduction, cellular damage, and viability decrease in sperm whereas metabolic capacity increased in adults. AVO combined with warming caused oxidative stress, cellular damage, genotoxicity, and decreased motility in sperm, while only antioxidant enzymes' activity was enhanced in adults. Overall, the present study demonstrated that when acting in combination the effects of both stressors were more prominent. Furthermore, considering the multiple-stressor scenario tested, major toxic effects occurred in male gametes in comparison to adults., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Author(s). Published by Elsevier B.V. All rights reserved.)

The main aim of this work was to evaluate the effect of intra-articular, sub-synovial steroid injections (IASSSI) with different doses during temporomandibular joint (TMJ) arthroscopy. Using a single-blind, randomized clinical trial, the investigators enrolled a sample of subjects who underwent TMJ arthroscopy with vs. without IASSSI for treating TMJ disorder (TMD). The predictor variable was the treatment group classified as no treatment (A - without IASSSI) or active treatments (IASSSI (B) with 2 mg or (C) 4-mg dexamethasone). Study medications were randomly assigned. Only subjects were blinded to treatment assignment. The main outcome variables included the following: 1) pain assessed up to postoperative day 30 with the McGill Pain Questionnaire and visual analog scale (VAS); 2) maximum mouth opening (MMO); and 3) analgesic and anti-inflammatory drug consumption. Other variables were demographic (age, sex) or pathologic (disease manifestations and classifications). Descriptive and bivariate statistics were computed. Statistical significance was set at p ≤ 0.5, one-tailed test of hypothesis. The sample comprised 36 patients (n = 12 per study group). In the active group with 4 mg corticosteroids, pain reduction measured by VAS (odds ratio [OR] = 0.18; p = 0.013) and McGill scale scores (OR = 0.15; p = 0.048) was significantly higher than in the control group. The McGill scale scores significantly decreased in both IASSSI groups, compared to the control group, at the final follow-up (all p < 0.012), and IASSSI was significantly associated with reduced analgesic consumption at postoperative days 7 and 14 (p = 0.003). However, there was no significant difference regarding MMO among the three groups. Within the limitations of the study, it seems that intra-articular, sub-synovial steroid injections (IASSSI) with 4 mg dexamethasone should be preferred whenever appropriate when pain relief is the priority of the procedure. Brazilian Registry of Clinical Trials (ReBec)- 28yb2g., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 European Association for Cranio-Maxillo-Facial Surgery. Published by Elsevier Ltd. All rights reserved.)

The rate of introduction of man-made habitats in coastal environments is growing at an unprecedented pace, as a consequence of the expansion of urban areas. Floating installations, due to their unique hydrodynamic features, are able to provide great opportunities for enhancing water detoxification through the use of sessile, filtering organisms. We assessed whether the application of sponges to floating pontoons could function as a tool for biomonitoring organic and inorganic pollutants and for improving water quality inside a moderately contaminated marina in the NW Mediterranean. Fragments of two common Mediterranean sponges (Petrosia (Petrosia) ficiformis and Ircinia oros) were fixed to either suspended natural fibre nets beneath a floating pontoon or to metal frames deployed on the sea bottom. We assessed the accumulation of organic and inorganic contaminants in sponge fragments and, in order to provide an insight into their health status, we examined changes in their metabolic and oxidative stress responses and associated microbiomes. Fragments of both sponge species filtered out pollutants from seawater on both support types, but generally showed a better physiological and metabolic status when fixed to nets underneath the pontoon than to bottom frames. P. (P) ficiformis maintained a more efficient metabolism and exhibited a lower physiological stress levels and higher stability of the associated microbiome in comparison with I. oros. Our study suggests that the application of sponges to floating pontoon represents a promising nature-based solution to improve the ecological value of urban environments., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Present in an increasing number of products, UV-filters are continuously discharged into aquatic environments. Despite potential risks for inhabiting organisms are recognized, the effects of UV-filter 4-methylbenzylidenecamphor (4-MBC) on marine invertebrates are poorly investigated. By combining in vitro/in vivo exposures through a multi-biomarker approach on sperms and adults, the present study evaluated how 4-MBC affect the mussel species Mytilus galloprovincialis, providing ecologically relevant information on organisms' responses. From the obtained results, considering mortality as endpoint, sperms revealed a greater sensitivity (EC 50 :347 μg/L) than adults (EC 50 : not calculable). From an ecotoxicological perspective, this resulted in a derived threshold concentration (LOEC) of 100 μg/L and 72 μg/L, respectively. Effects at the cell/molecular level were provided by general redox-status imbalance and oxidative stress. Sperms showed functional and structural impairments, hyperactivation and DNA damage, while adults showed physiological, metabolic/energetic dysfunctions, DNA damage and activation of oxidative and biotransformation enzymes. High 4-MBC bioaccumulation was also observed in exposed mussels (BCFs:14.0-32.0 L/kg). These findings suggest that 4-MBC may impair fitness and survival of the broadcast spawning mussel M. galloprovincialis, affecting reproduction success and population growth., (Copyright © 2022 Elsevier Inc. All rights reserved.)

Microplastic (MP) pollution represents a distinctive mark of the Anthropocene. Despite the increasing efforts to determine the ecological impacts of MP on marine biodiversity, our understanding of their toxicological effects on invertebrate species is still limited. Despite their key functional roles, sponges (Phylum Porifera) are particularly understudied in MP research. These filter-feeders extract and retain particles from the water column, across a broad size range. In this study, we carried out a laboratory experiment to assess the uptake of MPs (polyethylene, PE) by the Mediterranean sponge Petrosia ficiformis, how MPs influence key biological process after different times of exposure (24h and 72h) and whether they can be subsequently eliminated. MP uptake increased with time of exposure, with 30.6% of the inoculated MP particles found in sponge samples after 72h. MPs impaired filtration and respiration rates and these effects were still evident 72h after sponges had been transferred in uncontaminated water. Our study shows that time of exposure represents a key factor in determining MP toxicity in sponges. In addition, our results suggest that sponges are able to incorporate foreign particles and may thus be a potential bioindicator for MP pollutants., (Copyright © 2022 Elsevier Inc. All rights reserved.)

The acquisition of data to safeguard marine protected areas located close to ports is important in order to develop plans that allow effective protection from pollution as well as sustainable development of the port. The area Secche della Meloria is a Marine Protected Area (MPA-MEL) three miles from Livorno Harbour (LH), which is characterized by a long history of pollution. Here we studied the bioaccumulation and transcriptomic patterns of the marbled crab, Pachygrapsus marmoratus (Fabricius, 1787) (Crustacea; Brachyura, Grapsidae), inhabiting the two selected sites. Results showed that the two crab populations are significantly different in their chemical composition of trace elements and Polyciclic Aromatic Hydrocarbons (PAHs), and gene expression patterns (1280 DEGs). Enrichment analysis indicated that crabs at LH had the highest stress response genes, and they were associated with higher levels of bioaccumulation detected in body tissues. We are confident that the significant differential gene expression profiles observed between crabs, characterized by significant chemical differences, is associated with responses to contaminant exposure., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

With the increase of UV filters usage and consequent release into aquatic environments, the concerns about their potential ecological risks are also increasing. According to this, in the present study, adult polychaetes of the species Ficopomatus enigmaticus were chronically exposed to three concentrations (0.01, 0.1 and 0.5 mg/L) of organic and inorganic filters (Ethylhexyl methoxycinnamate (EHMC) and nanoparticulate Zinc oxide (nZnO), respectively) in order to analyse biochemical responses related to cellular damage, antioxidant defence, biotransformation mechanisms and, lastly, neurotoxicity. Despite major lipid peroxidation caused by EHMC was observed, both UV filters have produced the same response patterns. In details, a clear concentration-dependent activation of glutathione S-transferases and a significant decrease of acetylcholinesterase levels defined an important neurotoxic effect was observed for both contaminants. These results become important to expand the limited scientific literature on biochemical responses of marine and brackish water invertebrates to organic and inorganic UV filters., (Copyright © 2022. Published by Elsevier Ltd.)

In the last decades the use of rare earth elements (REEs) increased exponentially, including Terbium (Tb) which has been widely used in newly developed electronic devices. Also, the production and application of nanoparticles has been growing, being Carbon Nanotubes (CNTs) among the most commonly used. Accompanying such development patterns, emissions towards the aquatic environments are highly probable, with scarce information regarding the potential toxicity of these pollutants to inhabiting species, especially considering their mixture. In the present study the effects of Tb and CNTs exposure (acting alone or as a mixture) on native and invasive clams' species (Ruditapes decussatus and Ruditapes philippinarum, respectively) were evaluated, assessing clams' accumulation and metabolic capacities, oxidative status as well neurotoxic impacts. Results obtained after a 28-days exposure period showed that the accumulation of Tb in both species was not affected by the presence of the CNTs and similar Tb concentrations were found in both species. The effects caused by Tb and CNTs, acting alone or as a mixture induced greater alterations in R. philippinarum antioxidant capacity in comparison to native R. decussatus, but no cellular damages were observed in both species. Nevertheless, although metabolic impairment was only observed in clams exposed to Tb, loss of redox balance and neurotoxicity were evidenced by both species regardless the exposure treatment. These findings highlight the potential impacts caused by CNTs and Tb, which may affect clams' normal physiological functioning, impairing their reproduction and growth capacities. The obtained results point out the need for further investigation considering the mixture of pollutants., Competing Interests: Declaration of competing interest The Authors whose names are listed immediately below certify that they have NO affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; participation in speakers' bureaus; membership, employment, consultancies, stock ownership, or other equity interest; and expert testimony or patent-licensing arrangements), or nonfinancial /interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript., (Copyright © 2021 Elsevier B.V. All rights reserved.)

The transmission of antimicrobial resistance bacteria from animals to humans has become an important concern. The extended-spectrum beta-lactamase (ESBL) -AmpC- producing Escherichia coli (ESBL-AmpC EC) and quinolones resistant E. coli are of particular interest. The present study aimed to evaluate the load and prevalence of antibiotic-resistant commensal E. coli along the goose production cycle on 2 free-range farms in central Italy. On A farm, oxytetracycline was administered, while the B farm did not use antibiotics during the geese productive cycle. One hundred geese of 1-day-old from the same batch were divided into the two farms. At hatching, the animals showed an average of E. coli loads was 6.83 ± 0.48 log CFU/g, and 0.28 ± 0.28, 0, 5.12 ± 0.54 log CFU/g for E. coli resistant to nalidixic acid (E. coli nal ), to cefotaxime (E. coli cef ) and to tetracyclines (E. coli tet ), respectively. The loads of E. coli, E. coli nal , E. coli cef and E. coli tet on 224 environmental faecal pools were determined at 8 time points. Antimicrobial susceptibility and molecular characterization of E. coli cef isolates were performed. The ANOVA was used to assess the difference in bacterial loads between the two farms. We described more than 50% of resistances for tetracyclines in both farms, and sulphonamides and cephazolin in the A farm. The loads of E. coli and E. coli nal in faeces were estimated at approximately 6-7 log (CFU/g) and 5-6 log (CFU/g) in the two farms, respectively. The average load of extended-spectrum beta-lactamase Escherichia coli (ESBL EC) in goose faeces varied broadly along the production cycle: in the first weeks, a sharp increase was observed in both farms, while later on A farm, the burden of ESBL EC remained steady until the end of the production cycle and on B farm the load dramatically decreased from 6 wk of age onward. An increase in the proportion of E. coli nal was observed on A farm shortly after the antibiotic administration. Our study shows that the dynamics of antibiotic-resistant E. coli in farmed geese are similar to the ones observed in broilers. However, the risk of the emergence of antibiotic-resistant commensal E. coli, might be mitigated by the adoption of good management practices, including prudent use of antibiotics., (Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.)

Nowadays, multi-walled carbon nanotubes are considered to be emerging contaminants and their impact in ecosystem has drawn special research attention, while other contaminants, such as caffeine, have more coverage in literature. Despite this, the effects of a combination of the two has yet to be evaluated, especially considering predicted temperature rise. In the present study a typical bioindicator species for marine environment, the clam Ruditapes decussatus, and classical tools, such as biomarkers and histopathological indices, were used to shed light on the species' response to these contaminants, under actual and predicted warming scenarios. The results obtained showed that both contaminants have a harmful effect at tissue level, as shown by higher histopathological index, especially in digestive tubules. Temperatures seemed to induce greater biochemical impacts than caffeine (CAF) and -COOH functionalized multi-walled carbon nanotubes (f-MWCNTs) when acting alone, namely in terms of antioxidant defences and energy reserves content, which were exacerbated when both contaminants were acting in combination (MIX treatment). Overall, the present findings highlight the complex response of clams to both pollutants, evidencing the role of temperature on clams' sensitivity, especially to mixture of pollutants., (Copyright © 2021 Elsevier Inc. All rights reserved.)

Contamination by organic and inorganic compounds remains one of the most complex problems in both brackish and marine environments, causing potential implications for the reproductive success and survival of several broadcast spawners. Ficopomatus enigmaticus is a tubeworm polychaete that has previously been used as a model organism for ecotoxicological analysis, due to its sensitivity and ecological relevance. In the present study, the effects of five trace elements (zinc, copper, cadmium, arsenic and lead), one surfactant (sodium dodecyl sulfate, SDS) and one polycyclic aromatic hydrocarbon (benzo(a)pyrene, B(a)P) on the sperm quality of F. enigmaticus were investigated. Sperm suspensions were exposed in vitro to different concentrations of each selected contaminant under four salinity conditions (10, 20, 30, 35). Possible adverse effects on sperm function were assessed by measuring oxidative stress, membrane integrity, viability and DNA damage. Sperm quality impairments induced by organic contaminants were more evident than those induced by inorganic compounds. SDS exerted the largest effect on sperm. In addition, F. enigmaticus sperm showed high tolerance to salinity variation, supporting the wide use of this species as a promising model organism for ecotoxicological assays. Easy and rapid methods on polychaete spermatozoids were shown to be effective as integrated sperm quality parameters or as an alternative analysis for early assessment of marine and brackish water pollution., (Copyright © 2020 Elsevier Inc. All rights reserved.)

Previous studies suggested the suitability of the brackish-water serpulid (Ficopomatus enigmaticus) to be used as model organism for both marine and brackish waters monitoring, by the performance of sperm toxicity and larval development assays. The present study focused on larval development after the exposure of two F. enigmaticus populations (Mediterranean and Atlantic, collected in Italy and Portugal, respectively) to different trace elements (copper, mercury, arsenic, cadmium, and lead) at different concentrations. Results of larval development assays were presented as the percentage of abnormal developed larvae. The effect, measured in terms of EC 50 for all toxicants tested, showed that mercury was the most toxic metal for larvae of both populations. Specifically, the tested trace elements may be racked in the following order from the highest to the lowest toxicity: Mediterranean: mercury > copper > lead > arsenic > cadmium; Atlantic: mercury > copper > cadmium > arsenic > lead. Responses of both populations were similar for arsenic. Lead was the least toxic element for the Atlantic population, while cadmium showed the least toxicity for the Mediterranean population. These preliminary results demonstrate the sensitivity and suitability of the organisms to be used in ecotoxicological bioassays and monitoring protocols. Moreover, chemical analyses on soft tissues and calcareous tubes of collected test organisms and their sampling site water were performed, to identify and quantify the concentration of the tested trace elements in these 3 matrices. Populations exhibited less sensitivity to a certain element together with a relevantly higher concentration of the same element in soft tissues. This may indicate a certain resistance to particular contaminant toxic effects by organisms that tend to accumulate the same toxicant. This highlights the potential correlation between wild-caught test organisms' responses and a deep characterization of the sampling site to identify putative abnormalities or differences in model organism response during bioassay execution., (Copyright © 2020 Elsevier Inc. All rights reserved.)

The use of carbon nanotubes (CNTs) is rapidly increasing and several scientific studies have addressed their toxicological properties. However, only a very small number of publications have deal with the interaction between CNTs and other molecules. Triclosan (TCS) is an antibacterial agent used in personal care and household products. Commonly detected in aquatic ecosystems, there is a strong evidence that aquatic biota is sensitive to this compound. Aside from emergent pollutants, aquatic organisms are continuously subjected to abiotic variations including salinities. Therefore, the main goal of the present study was to better understand how physio-chemical interactions of CNTs with TCS under different salinity levels (37, 28 and 19) affect the mussel species Mytilus galloprovincialis through the evaluation of biochemical alterations on gametes (sperms) and adult tissues, providing more ecologically relevant information on organisms' responses. The results showed toxicological effects in terms of sperm metabolic activity and intracellular reactive oxygen species production as well as cellular damage and alteration of metabolic capacity at the adult's stage when exposed to both contaminants acting alone and in combination, under tested salinities. Moreover, when the mussels were exposed to the combination of both contaminants, they showed major toxic impacts on both assessed biological levels (adult tissues and sperms) especially under control salinity. This suggests that toxicity upon mixture exposure compared to single-substance exposure may impair mussels' populations, affecting reproduction success and growth., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Marine toxins in bivalves pose an important risk to human health, and regulatory authorities throughout the world impose maximum toxicity values. In general, bivalve toxicities due to paralytic shellfish toxins (PSTs) above the regulatory limit occur during short periods, but in some cases, it may be extended from weeks to months. The present study examines whether cockles (Cerastoderme edule), mussels (Mytilus galloprovincialis) and razor shells (Solen marginatus) naturally exposed to a bloom of Gymnodinium catenatum activated or suppressed biochemical responses as result of the presence of PSTs in their soft tissues. Toxins (C1+2, C3+4, GTX5, GTX6, dcSTX, dcGTX2+3 and dcNEO) and a set of biomarkers (ETS, electron transport system activity; GLY, glycogen; PROT, protein; SOD, superoxide dismutase; CAT, catalase; GPx, glutathione peroxidase; GST, glutathione S-transferases; LPO, lipid peroxidation; reduced (GSH) and oxidized (GSSG) glutathione contents and AChE, acetylcholinesterase activity) were determined in the three bivalve species. Specimens were harvested weekly in Aveiro lagoon, Portugal, along thirteen weeks. This period included three weeks in which bivalve toxicity exceeded largely the regulatory limit and the subsequence recovery period of ten weeks. Biochemical performance of the surveyed species clearly indicated that PSTs induce oxidative stress and neurotoxicity, with higher impact on mussels and razor shells than in cockles. The antioxidant enzymes CAT and GPx seemed to be the biomarkers better associated with toxin effects., (Copyright © 2020 Elsevier Inc. All rights reserved.)

Ficopomatus enigmaticus was adopted as model species for ecotoxicological bioassay, with its larval development as endpoint. Two different populations of the same species, collected in areas far from each other (Mediterranean Sea and Atlantic Ocean), were exposed to multi-walled carbon nanotubes, a class of emerging pollutants with a constantly increasing relevance in the landscape of nanomaterials production. Moreover, a molecular analysis based on Cyt b amplification and sequencing, was carried out to confirm that both populations belong to the same species. The aim of the present work was to strengthen existing results about F. enigmaticus relevance in ecotoxicological bioassays, adding the variable of population effect. For both populations the concentration-response curve of effect at different toxicant concentrations was similar and, at certain concentrations, overlapping, confirming the ecological relevance of the assay. These results posed an interesting acceptance on the introduction of this species as model in ecotoxicological bioassay scenery, underlining the relevance of a widespread wild species to compare effects of chemicals and environmental samples over large distances using the same bioassay., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

As a consequence of their unique characteristics, the use of Engineered Nanomaterials (ENMs) is rapidly increasing in industrial, agricultural products, as well as in environmental technology. However, this fast expansion and use make likely their release into the environment with particular concerns for the aquatic ecosystems, which tend to be the ultimate sink for this type of contaminants. Considering the settling behaviour of particulates, benthic organisms are more likely to be exposed to these compounds. In this way, the present review aims to summarise the most recent data available from the literature on ENMs behaviour and fate in aquatic ecosystems, focusing on their ecotoxicological impacts towards marine and estuarine bivalves. The selection of ENMs presented here was based on the OECD's Working Party on Manufactured Nanomaterials (WPMN), which involves the safety testing and risk assessment of ENMs. Physical-chemical characteristics and properties, applications, environmental relevant concentrations and behaviour in aquatic environment, as well as their toxic impacts towards marine bivalves are discussed. Moreover, it is also identified the impacts derived from the simultaneous exposure of marine organisms to ENMs and climate changes as an ecologically relevant scenario., (Copyright © 2019 Elsevier Inc. All rights reserved.)

The impact of gender and diet on the proteome of Longissimus dorsi was addressed by 2D-PAGE analysis of male and female pigs, fed with a barley-based control diet and a diet enriched with extruded linseed and plant extracts. No statistically significant difference in protein number between female and male samples was found. Furthermore, PCA excluded gender-dependent protein clusters. For both the control and enriched diet, several spots exhibited at least a 1.5-fold intensity difference, but none showed a statistically relevant variation. Protein profiles PCA for both diets indicated that the first two principal components account up to 47% of total variance, with two diet-dependent separated clusters. Among 176 common spots, 29 exhibited >1.5 fold change, mostly more abundant in the control diet. PMF identified 14 distinct proteins, including myofibrillar proteins, glycolytic enzymes and myoglobin, thus suggesting a diet-dependent meat quality. A statistically significant increase in carbonylated proteins of enriched diet samples was detected using the 2,4-dinitrophenylhydrazine method but not using fluorescein-5-thiosemicarbazide-labeled bands. ROS induction and DNA oxidative damage, detected in a human cell line exposed to digested meat from both diets, further support the notion that the enriched diet does not protect against oxidative stress. SIGNIFICANCE: The comparison of the protein profile of female and male Longissimus dorsi from pigs fed by a control diet and a diet enriched with polyphenols, indicate no gender effect, whereas diet affects the abundance of several proteins, possibly linked to meat quality. Protein carbonylation was statistically higher in meat from the enriched diet, suggesting that polyphenols at the concentration present in the diet did not exert a protective effect against oxidation., (Copyright © 2019 Elsevier B.V. All rights reserved.)

The need for reducing erosion and sediment transport, and protecting human settlements has urged the construction of channel control works in mountain streams. Such works include different structures, the check dams being probably the most widespread and archetypical. The performance of channel control works in mountain streams has usually been evaluated based on their effectiveness in stabilizing the channels and the adjacent hillslopes, and in retaining sediment. The widespread presence of channel control works in mountain streams of various regions, however, has also a significant impact on hillslope-channel coupling and on sediment transfer across the channel system. Taking into account the impact of channel control works on sediment connectivity at the catchment scale has remarkable importance for planning new control structures and managing the existing ones and, more in general, for sediment management in mountain catchments. In this paper, we consider the channel control works within the conceptual framework of sediment connectivity and related terminology, and we analyze the spatial and temporal scales of the interactions between channel control works and sediment coupling-decoupling. Some examples from the Italian Alps outline the impact of check dams and other hydraulic structures on sediment connectivity and show the potential of geomorphometry in assessing such impacts. The overall effect of control works on sediment connectivity consists of an alteration of the sediment cascade with a decrease in the efficiency of sediment transfer. Sediment is partly retained behind grade-control dams within the catchment or in sediment traps equipped with retention check dams near the alluvial fan apex. These works contribute to decouple the alluvial fans from the upslope catchment. On the contrary, channel bed lining on alluvial fans favors sediment transfer to the receiving river, but this effect usually involves small amounts of sediment not retained by the check dams built upstream., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Ocean acidification events are recognized as important drivers of change in biological systems. Particularly, the impacts of acidification are more severe in estuarine systems than in surface ocean due to their shallowness, low buffering capacity, low salinity and high organic matter from land drainage. Moreover, because they are transitional areas, estuaries can be seriously impacted by a vast number of anthropogenic activities and in the last decades, carbon nanomaterials (CNMs) are considered as emerging contaminants in these ecosystems. Considering all these evidences, chronic experiment was carried out, trying to understand the possible alteration on the chemical behaviour of two different CNMs (functionalized and pristine) in predicted climate change scenarios and consequently, how these alterations could modify the sensitivity of one the most common marine and estuarine organisms (the polychaeta Hediste diversicolor) assessing a set of biomarkers related to polychaetes oxidative status as well as the metabolic performance and neurotoxicity. Our results demonstrated that all enzymes worked together to counteract seawater acidification and CNMs, however oxidative stress in the exposed polychaetes to both CNMs, especially under ocean acidification conditions, was enhanced. In fact, although the antioxidant enzymes tried to cope as compensatory response of cellular defense systems against oxidative stress, the synergistic interactive effects of pH and functionalized CNMs indicated that acidified pH significantly increased the oxidative damage (in terms of lipid peroxidation) in the cotaminated organisms. Different responses were observed in organisms submitted to pristine CNMs under pH control, where the lipid peroxidation did not increase along with the increasing exposure concentrations. The present results further demonstrated neurotoxicity caused by both CNMs, especially noticeable at acidified conditions. The mechanism of enhanced toxicity could be attributed to slighter aggregation and more suspended NMs in acidified seawater (as demonstrated by the DLS analysis). Therefore, ocean acidification may cause a higher risk of CNMs to marine ecosystems., (Copyright © 2019 Elsevier B.V. All rights reserved.)

With the increased production and research on nanoparticles, the presence of carbon nanotubes (CNTs) in aquatic systems is very likely to increase. Although it has been shown that CNTs may cause toxicity in marine organisms, to our knowledge, the possible impacts under global temperature increase is still unknown. For this reason, biochemical and physiological impacts induced in Mytilus galloprovincialis due to the presence of functionalized multi-walled CNTs (f-MWCNTs) and increased temperature were investigated in the present study. The mussels exposed to increased temperature alone presented higher metabolic capacity and expenditure of glycogen as an energy resource to fuel up defense mechanisms and thus preventing oxidative damage. Contrarily, organisms exposed to f-MWCNTs alone seemed not stressed enough to demonstrate differences in the metabolism capacity. Furthermore, f-MWCNTs seemed not able to significantly activate their antioxidant and biotransformation enzymes, which in turn may led to oxidative damage in the cells especially when organisms were exposed to a warmer temperature. In fact, at higher temperature, the antioxidant response of organisms exposed to f-MWCNTs was not effective and oxidative damage levels were observed. Nevertheless, no additive or synergetic effects were observed when mussels were exposed to both stressors simultaneously., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Ficopomatus enigmaticus is a serpulid polychaete, reef-forming, dioecious gamete spawner throughout different seasons and in different salinity conditions. Due to these characteristics, its candidacy as a model organism to monitor both marine and brackish matrices was assessed. Marine sediments collected in a polluted area near the port of Ancona (Italy) were used as case study. Besides the chemical characterization of sediments, the larval development of F. enigmaticus was adopted as an assay for ecotoxicological assessment of sediments in addition to a regulatory bioassay battery with different organisms and endpoints. The quality of sediments was classified according to the Italian Ministerial Decree for sediment management (Ministerial Decree 173, 2016) by using the SEDI-QUAL-SOFT® (ver. 109.0) software developed by the Italian Environmental Protection Agency (ISPRA). The F. enigmaticus larval development assay showed a quite similar sensitivity to C. gigas larval development assays, indicating its potential use in a regulatory battery of bioassays., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Objective: This study aimed to analyze radiologic and clinical results with a minimum 5 years follow-up (FUP) of lateral lumbar total disc replacement for the treatment of symptomatic lumbar degenerative disc disease., Methods: We performed a prospective, single-center, clinical, and radiologic study. Patients were treated with lumbar total disc replacement (extreme lateral total disc replacement) by a lateral transpsoas approach. From 2005 to 2012, 60 patients were enrolled (31 male, 29 female; total, 66 levels; average age, 42.8 years [standard deviation (SD), 9.7 years, range, 22-64 years]; mean body mass index, 26.0 [SD, 3.4]). Clinical end points included visual analog scale and Oswestry Disability Index questionnaires, complications, and reoperation. Radiographic end points included heterotopic ossification (McAfee classification), adjacent level disease, and prosthesis migration or subluxation., Results: The mean surgical duration was 122 minutes (SD, 45 minutes) with mean 58 mL (SD, 21 mL) of estimated blood loss. No intraoperative complication occurred. The exceptions were 1 patient with postanesthesia apnea and 2 patients with quadriceps motor deficit (resolved within 4 months with physiotherapy). Of 60 patients, 9 were missed to FUP and 51 (85%) were enrolled in the study, with mean FUP of 92 months (range, 60-122 months). In total, 5 levels (9%; 5 of 55) required to be fused. Both removal of the prostheses and interbody fusion were performed by the lateral transpsoas approach. One patient experienced CrCo allergy (at 2 months); 4 experienced persistent pain from different causes (at 7, 9, 24, and 88 months). Five patients (10%) presented with progression at adjacent levels and 2 (4%) required surgery. One patient required sacroiliac fusion at 63 months. There were no complications during the retrieval surgeries. One partial disc migration occurred but the patient refused retrieval. There was no bone bridging in 9% of the discs (grade 0 heterotopic ossification): grade I, 22%; grade II, 31%; grade III, 20%; grade IV (fusion), 18%. Most heterotopic ossification cases (93%) occurred in the lateral aspect of the disc space, and mostly at the contralateral side of the surgical approach. Patient-reported outcomes significantly improved (P < 0.01) at the last FUP. Visual analog scale back pain score was preoperatively 8.5, early postoperatively 2.5, and at last FUP 3.1. Oswestry Disability Index was preoperatively 55%, early postoperatively 31%, and at last FUP 21%., Conclusions: This study presents mid-term to long-term results of extreme lateral total disc replacement artificial disc for the treatment of lumbar degenerative disease, with fast mobilization, sustained pain relief, and improved physical function. Despite the low rate of ALDis, some discs evolved to ankyloses and others were retrieved. Lumbar artificial disc replacement by the lateral approach seems to be a safe and effective treatment., (Copyright © 2018 Elsevier Inc. All rights reserved.)

Intertidal mussel species are frequently exposed to changes of environmental parameters related to tidal regimes that include a multitude of stressors that they must avoid or tolerate by developing adaptive strategies. In particular, besides air exposure during low tides, intertidal mussels are also subjected to warming and, consequently, to higher risk of desiccation. However, scarce information is available regarding the responses of mussels to tidal regimes, particularly in the presence of other stressors such as increased temperature. Investigating the impacts of such combination of conditions will allow to understand the possible impacts that both factors interaction may generate to these intertidal organisms. To this end, the present study evaluated the impacts of different temperatures (18 °C and 21 °C) on Mytilus galloprovincialis when continuously submersed or exposed to a tidal regime for 14 days. Results showed that in mussels exposed to increased temperature under submersion conditions, the stress induced was enough to activate mussels' antioxidant defenses (namely glutathione peroxidase, GPx), preventing oxidative damage (lipid peroxidation, LPO; protein carbonylation, PC). In mussels exposed to tides at control temperature, metabolic capacity increased (electron transport system activity, ETS), and GPx was induced, despite resulting in increased LPO levels. Moreover, the combination of tides and temperature increase led to a significant decrease of lipid (LIP) content, activation of antioxidant defenses (superoxide dismutase, SOD; GPx) and increase of oxidized glutathione (GSSG), despite these mechanisms were not sufficient to prevent increased cellular damage. Therefore, the combination of increased temperature and air exposure induced higher oxidative stress in mussels. These findings indicate that increasing global warming could be more impacting to intertidal organisms compared to organisms continuously submersed. Furthermore, our results indicate that air exposure can act as a confounding factor when assessing the impacts of different stressors in organisms living in coastal systems., (Copyright © 2018 Elsevier B.V. All rights reserved.)

In recent years, oxidative stress has been recognized as one of the most common effects of nanoparticles in different organisms. Ficopomatus enigmaticus (Fauvel, 1923), a member of a large family of serpulidae polychates, is an important encrusting organism in a diverse set of marine habitats, from harbours to coral reefs. This species has been previously studied for ecotoxicological purposes, despite the lack of reported studies on this species biochemical response after exposure to different pollutants. For these reasons, and for the first time, a set of biomarkers related to oxidative status were assessed in polychaetes after 28 days of exposure. Furthermore, polychaetes metabolic performance and potential neurotoxicity were investigated. Results clearly demonstrated induced toxicity in the filter-feeder polychaetes after exposure to nanoparticles. Indeed, CNTs altered the biochemical and physiological status of F. enigmaticus, both in terms of energy reserves (reduction of protein and glycogen contents), oxidative status (expressed as damage in cell function such as protein carbonyl content and lipid peroxidation) and activation of antioxidant enzymes defences (Glutathione reductase, Catalase, Glutathione peroxidase and Glutathione S-transferases activities). The present study showed for the first time that this species can be used as a model organism for nanoparticle toxicology., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Large floods may produce remarkable channel changes, which determine damages and casualties in inhabited areas. However, our knowledge of such processes remains poor, as is our capability to predict them. This study analyses the geomorphic response of the Nure River (northern Italy) and nine tributaries to a high-magnitude flood that occurred in September 2015. The adopted multi-disciplinary approach encompassed: (i) hydrological and hydraulic analysis; (ii) analysis of sediment delivery to the stream network by means of landslides mapping; (iii) assessment of morphological modifications of the channels, including both channel width and bed elevation changes. The spatial distribution of rainfall showed that the largest rainfall amounts occur in the upper portions of the catchment, with cumulative rainfall reaching 300 mm in 12 h, and recurrence intervals exceeding 100-150 years. The unit peak discharge ranged between 5.2 and 25 m 3  s -1  km -2 . Channel widening was the most evident effect. In the tributaries, the ratio between post-flood and pre-flood channel width averaged 3.3, with a maximum approaching 20. Widening was associated with channel aggradation up to 1.5 m and removal of riparian vegetation. New islands formed due to the fragmentation of the former floodplain. In the Nure River, the average width ratio was 1.7, and here widening occurred mainly at the expenses of islands. Bed level dynamics in the Nure were varied, including aggradation, incision, and overall stability. The flood geomorphic effectiveness was more pronounced in the middle-higher portions of the basin. Planimetric and elevation changes were well correlated. Regression analysis of the relationship between widening and morphological/ hydraulic controlling factors indicated that unit stream power and confinement index were the most relevant variables. The study provides useful insights for river management, especially with regard to the proportion of the valley floor subject to erosion and/or deposition during large events., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Salinity plays a fundamental role in naturally fluctuating environments such as estuaries influencing physiological and biochemical performance of inhabiting biota. Moreover salinity is considered one of the main factors influencing nanoparticles' stability. Thus, the aim of the present paper was to show the impacts induced by different salinities (control-28 and 21) on the chemical behavior of water dispersible multi-walled carbon nanotube (MWCNTs-COOH) and the consequent toxicity in the common ragworm Hediste diversicolor, after long term exposure. Results showed a concentration-dependent toxicity in terms of energy reserves and metabolism, oxidative status and neurotoxicity. In addition, under low salinity (21), the toxicity of the carbon NMs was similar to the impacts measured under control (28), although under salinity 28 the concentrations of MWCNTs-COOH used generated greater alterations in LPO levels and antioxidant enzymes (SOD and GPx). These results demonstrate that higher salinity caused the formation of large-size aggregates, which increased the chance of physical retention, such as gravitational sedimentation, interception and straining of f-MWCNTs generating higher cell injuries than the impacts induced in polychaetes sensitivity to these contaminates due to low salinity., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Although an increasing number of studies have been published on the effects of emergent pollutants such as carbon nanoparticles, there is still scarce information on the impact of these contaminants on marine organisms when acting in combination with classical pollutants such as meta(loid)s. The present study evaluated the impacts of Arsenic and Multi-Walled Carbon Nanotubes (MWCNTs) in the clam Ruditapes philippinarum, assessing the effects induced when both contaminants were acting individually (As, NP) and as a mixture (As + NP). Metabolic capacity (electron transport system activity), oxidative stress (antioxidant and biotransformation enzymes activity and cellular damage) and neurotoxicity (Acetylcholinesterase activity) biomarkers were evaluated in clams after a 28 days exposure period. The results obtained showed that the accumulation of As was not affected by the presence of the NPs. Our results demonstrated that higher injuries were noticed in clams exposed to NPs, with higher metabolic depression and oxidative stress, regardless of the presence of As. Furthermore, higher neurotoxicity was observed in clams exposed to the combination of both contaminants in comparison to the effects of As and NPs individually., (Copyright © 2018 Elsevier B.V. All rights reserved.)

Due to unique chemical and physical properties, nanomaterials from the Graphene family are being increasingly introduced in all fields of science. The specific roles they can occupy within different applications are attracting increased attention by several industrial sectors. These carbon nanoparticles are released into the environment especially accumulating in aquatic systems. Since the discovery of graphene, a number of research actives are being conducted to find out the toxic potential of the Graphene family materials to different organism's models. Although their toxicity effects are well described for biomedical applications, few data were produced with the specific aim of assessing the toxic effects of these carbon nanomaterials in the aquatic environment. The purpose of this review is to compile up-to-date information on properties, applications and characterization methods of graphene family materials in aquatic environments and identified biological toxic impacts of these NMs, with special focus on graphene oxide based on the most recent literature., (Copyright © 2018. Published by Elsevier B.V.)

Despite of the large array of available carbon nanotube (CNT) configurations that allow different industrial and scientific applications of these nanoparticles, their impacts on aquatic organisms, especially on invertebrate species, are still limited. To our knowledge, no information is available on how surface chemistry alteration (functionalization) of CNTs may impact the toxicity of these NPs to bivalve species after a chronic exposure. For this reason, the impacts induced by chronic exposure (28days) to unfunctionalized MWCNTs (Nf-MWCNTs) in comparison with functionalized MWCNTs (f-MWCNTs), were evaluated in R. philippinarum, by measuring alterations induced in clams' oxidative status, neurotoxicity and metabolic capacity. The results obtained revealed that exposure to both MWCNT materials altered energy-related responses, with higher metabolic capacity and lower glycogen, protein and lipid concentrations in clams exposed to these CNTs. Moreover, R. philippinarum exposed to Nf-MWCNTs and f-MWCNTs showed oxidative stress expressed in higher lipid peroxidation and lower ratio between reduced and oxidized glutathione, despite the activation of defense mechanisms (superoxide-dismutase, glutathione peroxidase and glutathione S-transferases) in exposed clams. Additionally, neurotoxicity was observed by inhibition of Cholinesterases activity in organisms exposed to both MWCNTs., (Copyright © 2017 Elsevier B.V. All rights reserved.)

The present study evaluated the impacts of predicted seawater acidification and Hg pollution, when stressors were acting alone and in combination, on the polychaete Hediste diversicolor. Polychaetes were exposed during 28days to low pH (7.5), Hg (5μg/L) and pH7.5+Hg, and physiological alterations (respiration rate), biochemical markers related to metabolic potential (glycogen and protein content, electron transport system activity) and oxidative status (activity of antioxidant and biotransformation enzymes, lipid peroxidation) were evaluated. The results obtained clearly showed that polychaetes were sensitive to low pH and Hg contamination, both acting alone or in combination. Organisms used their energy reserves under stressful conditions, which decreased by up to half of the control content, probably to fuel defence mechanisms. Our findings further demonstrated that polychaetes exposed to these stressors presented increased antioxidant defence mechanisms (3 fold compared to control). However, organisms were not able to prevent cellular damage, especially noticed at Hg exposure and pH7.5. Overall, although all the tested conditions induced oxidative stress in Hediste diversicolor, the combined effect of seawater acidification and Hg contamination did not induce higher impacts in polychaetes than single stressor exposures. These findings may indicate that predicted climate change scenarios may not increase Hediste diversicolor sensitivity towards Hg and may not significantly change the toxicity of this contaminant to this polychaete species., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Multi-walled carbon nanotubes (MWCNTs) are one of the most important carbon Nanomaterials (NMs). The production and use of these carbon NMs is increasing rapidly and, therefore, the need to assess their presence in the environment and associated risks has become increasingly important. However, limited literature is available regarding the impacts induced in aquatic organisms by this pollutant, namely in invertebrate species. Diopatra neapolitana and Hediste diversicolor are keystone polychaete species inhabiting estuaries and shallow water bodies intertidal mudflats, frequently used to evaluate the impact of environmental disturbances in these systems. To our knowledge, no information is available on physiological and biochemical alterations on these two species due to MWCNTs exposure. Thus, the present study aimed to assess the toxic effects of different MWCNTs concentrations (0.01; 0.10 and 1.00mg/L) in both species physiological (regenerative capacity and respiration rate) and biochemical (energy reserves, metabolic activities, oxidative stress related biomarkers and neurotoxicity markers) performance, after 28 days of exposure. The results obtained revealed that exposure to MWCNTs induced negative effects on the regenerative capacity of D. neapolitana. Additionally, higher MWCNTs concentrations induced increased respiration rates in D. neapolitana. MWCNTs altered energy-related responses, with higher values of electron transport system activity, glycogen and protein concentrations in both polychaetes exposed to this contaminant. Furthermore, when exposed to MWCNTs both species showed oxidative stress with higher lipid peroxidation, lower ratio between reduced and oxidized glutathione, and higher activity of antioxidant (catalase and superoxide dismutase) and biotransformation (glutathione-S-transferases) enzymes in exposed organisms., (Copyright © 2016 Elsevier Inc. All rights reserved.)

Coralline algae are calcareous algae able to build biogenic structures, thus playing a key-role as marine biodiversity promoters and calcium carbonate producers. The aim was to estimate the growth of Ellisolandia elongata under thermal stress. E. elongata were cultured for 2, 4 and 6 months under "natural" temperature (Tc) and increased temperature (Ti = Tc + 3 °C). In order to determine a possible culturing effect, growth in the field was also measured. For the first time, Alizarin Red S dye was used in high energy shallow water environments. Thallus linear extension was higher in the cultured specimens (Tc and Ti) compared to the field specimens. The carbonate mass in the field was higher than in Ti and Tc after 2, 4 months but decreased after 6 months. Partly unknown in situ environmental factors could have affected growth and calcification rates in the field while thermal adaptation could explain growth rates in the culturing experiment., (Copyright © 2015 Elsevier Ltd. All rights reserved.)

In vitro assessment of the functional responses of leukocytes sometimes requires their isolation from blood, joint and tissues. In this study, we compared the efficiency of two procedures - the gelatin method and Ficoll-Hypaque density centrifugation gradient - to isolate peripheral blood neutrophils of healthy individuals and patients with active rheumatoid arthritis (RA). We also assessed whether these procedures affect the neutrophil activation status. Both purification procedures were concluded in 90min, and yielded cell populations with similar degrees of purity (80-90%), number of neutrophils (1-2×10(6) cells per mL of blood), and viability (97-100%). In vitro neutrophil priming with granulocyte-macrophage colony-stimulating factor (GM-CSF) significantly increased the reactive oxygen species producing ability of the cells stimulated with n-formyl-methionyl-leucyl-phenylalanine (n-fMLP), soluble immune complexes (s-ICs), and insoluble immune complexes (i-ICs). Isolated neutrophils not treated with GM-CSF responded to n-fMLP and i-IC, but not to s-IC. Almost all of the neutrophils (98-100%) purified by both methods expressed FcγRII/CD32 and FcγRIII/CD16, but they did not express significant levels of FcγRI/CD64. Similar results were obtained for healthy individuals' and RA patients' neutrophils. In summary, the gelatin method was comparable to Ficoll-Hypaque gradient in terms of purity, yield, and viability of the neutrophil preparations. Both methods neither primed or activated the neutrophils, nor affected their functional responsiveness. Therefore, both methods are suitable to isolate peripheral blood neutrophils of healthy individuals and RA patients., (Copyright © 2014 Elsevier B.V. All rights reserved.)

Susceptibility to infections, autoimmune disorders and tumor progression is strongly influenced by the activity of the endocrine and nervous systems in response to a stressful stimulus. When the adaptive system is switched on and off efficiently, the body is able to recover from the stress imposed. However, when the system is activated repeatedly or the activity is sustained, as during chronic or excessive stress, an allostatic load is generated, which can lead to disease over long periods of time. We investigated the effects of chronic cold stress in BALB/c mice (4°C/4 h daily for 7 days) on functions of macrophages. We found that chronic cold stress induced a regulatory phenotype in macrophages, characterized by diminished phagocytic ability, decreased TNF-α and IL-6 and increased IL-10 production. In addition, resting macrophages from mice exposed to cold stress stimulated spleen cells to produce regulatory cytokines, and an immunosuppressive state that impaired stressed mice to control Trypanosoma cruzi proliferation. These regulatory effects correlated with an increase in macrophage expression of 11β-hydroxysteroid dehydrogenase, an enzyme that converts inactive glucocorticoid into its active form. As stress is a common aspect of modern life and plays a role in the etiology of many diseases, the results of this study are important for improving knowledge regarding the neuro-immune-endocrine interactions that occur during stress and to highlight the role of macrophages in the immunosuppression induced by chronic stress., (Copyright © 2011 Elsevier Inc. All rights reserved.)

The safe administration of conscious sedation in a patient with a critical airway who underwent awake tracheotomy using dexmedetomidine, a selective alpha(2)-agonist with sympatholytic, anxiolytic, analgesic, and sedative properties, is presented. Unlike other commonly used sedative agents, dexmedetomidine provided adequate sedation with minimal respiratory depression., (Copyright 2010 Elsevier Inc. All rights reserved.)

We investigated in the chick whether the diet-induced changes of the hepatic content of cholesteryl esters (CE) influence the synthesis and the secretion of apoB- and apoA-I-containing lipoproteins. Control chicks received a low cholesterol diet for 2 (SD-1), 4 (SD-2), or 7 (SD-3) weeks; the chicks in the experimental groups received a cholesterol-rich diet for 2 weeks and were killed at the end of the cholesterol feeding (CH-F), and after 2 (CH-D) or 5 (CH-DD) weeks of a low cholesterol diet. Hepatic CE content in CH-F chicks was 30-fold that observed in controls, but returned to the control level after 5 weeks of cholesterol depletion (CH-DD). The incorporation of 35S-labeled amino acids into cell and medium apoB and apoA-I was measured in liver slices. Intracellular 35S-labeled apoB was similar in all groups whereas medium 35S-labeled apoB was 2-fold higher in CH-F than in controls (SD-1). Pulse-chase experiments showed that radioactive apoB secreted by CH-F chicks at 120 min of chase was 2 times that of SD-1 chicks. This increased secretion of apoB was not found in CH-D chicks. In CH-F chicks, the intracellular and medium 35S-labeled apoA-I were 2-fold the values found in controls (SD-1); apoA-I production returned to the control level only after 5 weeks of cholesterol depletion (CH-DD). The increased secretion of apoB and apoA-I in CH-F chicks was associated with an increased secretion of very low, intermediate, and low density lipoproteins containing newly synthesized apoB and apoA-I and of high density lipoproteins containing predominantly apoA-I. Thus, in response to hepatic CE accumulation induced by cholesterol feeding, a larger proportion of newly synthesized apoB is driven to the secretory pathway and more apoA-I is synthesized. This promotes an increased secretion of plasma lipoproteins that contribute to the removal of CE from the liver.

In the chick, the large cholesteryl ester (CE) store present in the liver during the last period of embryonic life increases at hatching and is rapidly depleted after 2-7 days of postnatal life. In this study we asked whether these changes were associated with variations in the hepatic production of apoB-containing lipoproteins. Liver slices taken from chicks at -3, 0 (hatching), 2, 4, 7, and 10 days of development were incubated with [35S]methionine in steady state incubations. ApoB production (cell + medium radioactivity) decreased from day -3 to day 0 (40%), increased at day 4 (54%), and decreased afterwards (45%). At day 4 the amount of 35S-labeled apoB-containing lipoproteins (VLDL-LDL) secreted into the medium was 1.7- and 1.5-times that found at days 0 and 7, respectively; the radioactivity incorporated into medium HDL (containing predominantly apoA-I) was 1.7-times that found at days 0 and 7. The incubation of liver slices with [3H]oleate showed that CE production at days 4 and 7 was 58% and 33%, respectively, of that found at day 0. The percentage of newly synthesized hepatic CE secreted into medium lipoproteins was 2.4%, 3.1%, and 2.2% at days 0, 4, and 7, respectively. The percentage of lipoprotein CE present in VLDL-LDL ranged from 38% at day 0 to 21% at day 7, and that present in HDL ranged from 62% at day 0 to 79% at day 7. To define whether the changes in the production of apoA-I- and apoB-containing lipoproteins were due to variations in apoB and apoA-I synthesis, the initial synthetic rate (pulse-labeling) and the mRNA content of these apolipoproteins were investigated. The initial apoB synthetic rate decreased 1.5-fold from day -3 to day 0, remained stable up to day 7, and decreased at day 10. Hepatic apoB mRNA followed a similar trend. The synthesis of apoA-I increased 2-fold from days -3/2 up to day 4 and did not change afterwards. In conclusion the increased hepatic CE content at hatching reflects a decreased production of apoB, while the depletion of CE observed from day 2 to day 7 is associated with an increased production of both apoB- and apoA-I-containing lipoproteins. The decreased apoB production at hatching is due to a decreased apoB synthesis whereas the increased apoB production at day 4 appears to be related to a post-translational event.

Multiple myeloma and benign monoclonal gammopathies are regarded as monoclonal B cell proliferations in which B lymphocyte maturation is blocked in the final stages of the differentiation cycle. "Blocked" cells accumulate in the body and produce large quantities of immunoglobulins. These are monoclonal, because they come from a monoclonal cell stock. This study presents the results of peripheral B lymphocyte and marrow plasma cell typing designed to reveal the postulated isotypical relationship of the proliferating cells and the serum paraprotein. A good, though not absolute correlation between the two immunoglobulins was noted in most patients. The data, however, was not used in the diagnosis and treatment of these diseases. Further studies with MoAb's will, perhaps, provide a finer subclassification of the B lymphocyte proliferation diseases and assist in their diagnosis and treatment.

Preliminary results obtained with OK anti-T and FMC anti-B monoclonal antibodies in a study of peripheral blood lymphocytes from eight patients with Waldenström's macroglobulinemia and of bone marrow lymphocytes from three patients, are reported. Endocytoplasmic immunofluorescence revealed a 1 to 3% marrow plasma cell fraction in all three cases, together with an approximately 10% increase in the monoclonal precursor compartment. Displacement of the peripheral blood helper: suppressor ratio was also observed using anti-T-lymphocyte monoclonal antibodies.