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There are many methods for assessing chromosomal stability, including the classical cytogenetic approaches described here, SNP genotyping described in Chapter 14, and the SKY, and FISH methods described in the previous edition of this manual. These methods differ in resolution and the types of abnormalities they can detect. The normal resolution obtainable by classic cytogenetic methods is estimated to be in the megabase range, while single nucleotide polymorphism (SNP) can give 2 kb resolution (Chapter 14). However, more resolution is not necessarily better; for example, SNP genotyping cannot be used to detect balanced translocations or inversions. Here we describe a simple, straightforward method for classical karyotyping of hPSCs.