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Lead (Pb), a toxic metal, causes severe health hazards to both humans and plants due to environmental pollution. Biochar addition has been efficiently utilized to enhance growth of plants as well as yield in the presence of Pb-induced stress. The present research introduces a novel use of biochar obtained from the weed Achyranthes japonica to enhance the growth of plants in Pb-contaminated soil. An experiment was performed with 7 treatments: Control, Pb2+ (10 mg kg−1) only, biochar (4 %) only, allantoin (4 g kg−1) only, biochar combined with Pb2+, allantoin combined with biochar, as well as a combination of allantoin and biochar with Pb2+. Lead toxicity alone markedly diminished plant growth metrics, including root and shoot length, biomass (wet and dry), chlorophyll concentration, and grain production. The application of biochar, allantoin, or their joint administration markedly enhanced the length of shoots (by 50.3 %, 29 %, and 70 %), length of roots (by 69 %, 50 %, and 69 %), and fresh biomass of shoots (by 5 %, 29 %, and 5 %), respectively. This enhancement is ascribed to improved soil characteristics and more efficient absorption of nutrients. The application of biochar, allantoin and their combination improved the tolerance against Pb2+ by increasing the total chlorophyll level by 12 %, 16 %, and 17 %, respectively, vs. the control. Likewise, these amendments significantly (p

This study aimed to investigate the effect of red grape pomace (RGP) treated with ozone (O3) gas as a substitute of low-quality alfalfa hay on the ruminal fermentation, nutrient digestibility, and milk production performance of fat-tailed, fed RGP substituted high fibrous alfalfa hay. Present study was performed in two experimental phases: 1) In situ and in vitro phase: Four Ghezel ewes in their third gestation (30 months old; weighing 51 ± 2 kg) with surgically implanted cannulas in their rumen were used to assess the in situ degradability and to obtain ruminal fluid to assay the in vitro gas production; the treatments were RGP not-processed (control; T0), and RGP processed with O3 for 6 (T1), 12 h (T2), and 24 h (T3). Processing RGP with O3 gas reduced (P

Guanidinoacetic acid (GAA), a precursor of creatine, is partially degraded in the rumen when unprotected. While GAA has shown promise as a feed additive, its optimal dose for ruminants remains unclear. This study investigated the dose-response effects of GAA on in vitro ruminal fermentation and greenhouse gas production in fattened sheep. Rumen inoculum from two Pelibuey sheep (30 kg ± 2 kg) fed a 50:50 forage: concentrate diet was used to test seven GAA doses: 0 (GAA0, control), 500 (GAA0.5), 1000 (GAA1), 1500 (GAA1.5), 2000 (GAA2), 2500 (GAA2.5), and 3000 (GAA3) mg/kg DM (dry matter). A completely randomized design was employed. GAA0.5 produced significantly higher total gas than GAA2. The CH4 production was similar across doses, except for GAA2.5, which was significantly higher than GAA0 and GAA0.5. GAA exhibited quadratic relationships with total gas production and linear relationships with CH4 and CO production but no significant effect on H2S. GAA linearly increased DM digestibility without significantly affecting ruminal fermentation profiles. GAA inclusion strongly influenced crude protein (CP) digestibility and degradability kinetics, with varying effects at different time points. The rate constant for degradation of the insoluble but potentially degradable CP fraction was highest with GAA1. In conclusion, GAA supplementation at GAA0.5 improved total gas production without increasing CH4 emissions, implying enhanced fermentation efficiency and improved performance. GAA's impact on CP digestibility varied with incubation time. These findings provide insights into developing optimal GAA dose/level supplementation that is effective in fattening sheep, though further in vivo studies are warranted to validate these effects and explore long-term impacts on rumen microbial populations and protein utilization.

Honey, beeswax, and propolis have long been recognized for their significance in the treatment and prevention of diseases. Numerous studies have demonstrated their antioxidant properties, making it crucial to investigate in depth their physicochemical, antioxidant, and microbial characteristics. The objective of this research was to examine the characteristics of propolis sourced from different areas within Iran. Propolis samples were obtained from beehives located in Sarab, Maku, Quchan, Neka, Qom, and Sanandaj regions for analysis. The physicochemical and antioxidant properties and mineral content of propolis were evaluated. Obtained data showed that Quchan propolis has the highest total antioxidant level, and the lowest amount was found for Qom samples (0.848 vs 0.531 %). Qom propolis samples had the highest aluminium, calcium and magnesium content among region samples. At a concentration of 1 % propolis, the Qom exhibited significant antibacterial efficacy against E. coli and S. aureus. The Quchan samples had more antibacterial activity against E. coli at a level of 2 % propolis. The results of this study showed that the chemical composition, mineral content and antibacterial properties of propolis can be different based on the region. This finding can trigger measures to further enhance the quality of a given provenience.

Thirty dairy buffaloes were used for 90 days to evaluate the impact of a novel protein source (HI-PRO®), a feed alternative rich in protein (made from Saccharomyces and Bacillus), on the productivity of nursing buffaloes. The nursing water buffaloes had an average weight of 550 ± 11.2 kg. The treatments consisted of diets containing two distinct protein sources: (1) soybean meal (44 % as the control group); and (2) HI-PRO® product. Throughout the trial, measurements of nutritional digestibility and blood metabolites (total protein, albumin, urea, and creatinine) were conducted. Quantity and composition of the milk were measured to determine the content of milk proteins, lipids, and lactose. The outcome of the results showed insignificant decrease in the buffaloes receiving HI-PRO® feed versus control group. Furthermore, there was enhancement in the fiber digestibility by approximately 2.5 % in the HI-PRO® group compared to the control group. The levels of protein, albumin, globulin, urea, and creatinine fall within the normal range for animals in good health. Using HI-PRO® resulted in a marginal improvement in milk production, increasing it by approximately 4.8 % compared to soybean meal. Furthermore, the buffaloes fed HI-PRO® showed a slightly elevated 4 % fat-corrected milk output and milk composition. To summarize, nursing buffaloes can utilize HI-PRO® as an efficient protein source in their diets, replacing soybean meal.

Saponins, the primary components of Yucca schidigera extract (YSE), are known to influence microbial activity in the rumen, which can affect various fermentation parameters. Moreover, encapsulating YSE with chitosan (CS) at varying carbohydrate levels (CHO) adds another layer of complexity that can provide valuable insights into the use of additives in mitigating greenhouse gases. This study investigated the impact of both crude and encapsulated forms of YSE on the production of ruminal anaerobic gases in cattle fed different levels of CHO-based diets. Ruminal contents were obtained from four slaughtered, crossbreed bulls (Charollais × Limousin) with a live weight of 400 ± 25 kg. The experimental design followed a completely randomized factorial arrangement, with factors including CHO level (25 %, 45 %, and 55 % DM), YSE forms (without extract, CS, crude, and nano-capsules), and dose of extract (0-, 0.25-, 0.5- and 1.0- mL/g of DM). Results showed that the type of extract significantly affected asymptotic total gas production, methane (CH4), hydrogen sulfide (H2S), carbon monoxide (CO), and dry matter digestibility. Nano-chitosan increased gas production and exhibited greater efficiency in reducing CH4 production by up to 61.4 %. While crude YSE reduced CH4 production by 38 %, nano-capsules increased production by 17.7 %–42.8 %. Furthermore, a significant interaction effect among CHO levels, type of extract, and extract dose was observed, particularly impacting H2S and CH4 production after 48 h of incubation, alongside an increase of about 25.3 % in metabolizable energy compared to the control. The use of CS and YSE improved (p

This study aimed to evaluate the dietary supplementation of Nannochloropsis oculata (N. oculata) microalgae in different levels (1-, 2-, 3-, 4-, and 5- g/100 g dry matter (DM)) on the kinetics of gas production (GP), methane (CH4) production, dry matter apparent digestibility, volatile fatty acids (VFA), and microbial population by in vitro method. The findings of this study indicated that adding N. oculata at levels of 1–5 g/100 g DM did not have an impact on GP at different incubation hours. However, a diet with 5 g/100 g DM N. oculata resulted in a consistent increase in GP rate (part c) compared to the control treatment (P

The effect of incorporating mealworm frass (MWF) in the diets of growing Gabaly rabbits on nutrient digestibility, live body weight and gain, properties of the carcass, and blood parameters was investigated. Fifty-two Gabaly rabbits (6 weeks old) were selected after weaning and divided into four groups in a random fashion (13 rabbits/group) based on their weights (653 ± 15 g). The experimental period extended from 6 to 15 weeks of age. Four isocaloric, isonitrogenous and isofibrous diets in pellet shape were formulated. Mealworm frass (MWF) was incorporated at levels of 0, 1, 2, and 3 % for rabbit groups: G0 (as a control), G1, G2, and G3. At the end of the feeding experiment period (15 weeks of age), digestibility trials were conducted to determine the nutrient digestibility and nutrition values of the experimental diets. Three rabbits were slaughtered from each group to determine the carcass traits and some blood parameters. The chemical composition MWF was recorded as dry matter 87.08-, organic matter 84.98-, crude protein 22.59-, crude fibre 17.62-, ether extract 2.78-, ash17.24-, and nitrogen-free extract 41.99- %. The digestibility of dry matter, organic matter, crude protein, and crude fibre improved (P

Biopolymer films (biofilms) were evaluated for suitability in meat packaging applications. Polyesters, proteins, and carbohydrates are among the most commonly utilized materials for producing bioplastics due to their mechanical properties, which closely resemble those of conventional plastics. However, these properties are significantly influenced by the film's composition, molecular weight, solvent type, pH, component concentration, and processing temperature. Conventional plastic films, including microplastics, are non-bioactive and contribute to persistent pollution. This underscores the importance of developing novel materials that incorporate bioactive plant compounds to endow plastic films with antimicrobial and antioxidant functionalities. In this study, two gelatin-chitosan films were fabricated: one without any extract and another incorporating guava leaf extract. These films were characterized to determine their optical, mechanical, morphological, and thermal properties. Additionally, a microbiological analysis was conducted to assess the impact of polymeric biofilm packaging on the shelf life of beef. Both films exhibited favorable tensile strength values (24.74–27.12 MPa), high transparency (0.79–1.18), and effective barrier properties against water vapor (8.95–9.29 × 10⁻⁸ g·mm·Pa⁻1 h⁻1 m⁻2) and oxygen (9.7–9.10 × 10⁻1⁸ m³·m⁻2 s⁻1 Pa⁻1). During storage, the biofilm containing guava leaf extract demonstrated a reduction in microbial growth, and this enhanced the beef’s color stability. In conclusion, biopolymeric films incorporating guava leaf extract demonstrated notable antioxidants and antimicrobial properties, effectively inhibiting microbial growth, preserving the physical quality of beef, and significantly extending its shelf life under refrigerated conditions.

Innovative integrated approaches of nanoparticles (NP) and plant growth-promoting bacteria (PGPB) can accelerate plant growth and remediation of cadmium (Cd) contaminated soil. The effectiveness of the combination and interactions amongst Cd-resistant PGPB, ryegrass (Lolium perenne), graphene nanoplatelets (GNPs), and graphitic carbon nitride (g-C3N4) nanomaterials have not yet been elucidated. Cd-spiked soil was supplemented with GNPs, g-C3N4, and inoculated with bacterial strains (Serratia bozhouensis and Serratia marcescens). The PGPB and GNPs combination produced the highest biomass, enhancing the accumulation of Cd in ryegrass. The highest Cd uptake (92 %) by ryegrass was observed in Serratia bozhouensis and GNPs treatments. The reasons for increased plant growth and Cd-phytoremediation by the PGPB-GNPs combination are explained. GNPs application promoted PGPB survival in the rhizosphere. Inoculant strains showed a high relative abundance of >82 % in the inoculated treatments. The exchangeable Cd fraction significantly increased in PGPB-GNPs treatments. The highest exchangeable Cd (80 %) was noted with Serratia bozhouensis and GNPs. PGPB also increased the dehydrogenase, catalase, phosphatase, and urease enzyme activity in soil. The PGPB-GNPs combination also reduced the Cd-induced stress injury to ryegrass through increased production of antioxidants. The present work supports our understanding of the synergistic effects of nanomaterials, bacteria, and plants, thus boosting their role in restoring heavy metal-contaminated soils.

ABSTRACT: The study aimed to assess the effects of different dietary Silybum marianum (SM, milk thistle) powder levels on growth performance, productivity, immunity, small intestine, haemato-biochemical parameters, meat quality, and egg and carcass characteristics of laying quails. The experimental subjects consisted of one hundred and eight 43-day-old quails divided into 3 treatments (0, 0.75, and 1.50% SM) with 4 replicates each. The egg characteristics and growth performance of the quails were evaluated. Quails were euthanized for evaluation of carcasses, microbiota, and sensory characteristics of meat. Blood samples were analyzed for haematology and biochemical profile. SM at 0.75% and 1.50% significantly (P < 0.05) increased feed intake, enhanced egg characteristics (number, weight, width, length, volume, weight of egg yolk, and eggshell thickness), jejunum and ileum length, spleen weight, lactobacillus population, sensory characteristics of meat, red blood cell (RBC), hemoglobin, erythrocytic indices, concentration of albumin, globulin and thyroid stimulating hormone (TSH). SM at 0.75% and 1.50% decreased (P < 0.05) carcass weight (abdominal fat, heart, neck, and pancreas), feed conversion ratio (FCR) based on eggs produced, percentages of heterophils and lymphocytes, concentration of lactate dehydrogenase, population of coliforms clostridia, and Escherichia coli. Aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, and creatine kinase were not significantly (P > 0.05) altered by 0.75% and 1.50% SM. SM at both levels (0.75% and 1.50%) may improve growth, egg characteristics, immune response, intestinal morphology and microbiota, meat quality and erythropoiesis, and also lead to decreased cholesterol in laying quails. Economics can be improved, too. The authors recommend adding 1.0% of SM to quail diet.

Significant efforts have been made to understand how fat deposition in sheep tail is regulated in genetic, transcriptomic, physiologic, biochemical, and metabolic levels in order to elucidate the complex mechanisms underlying the energy storage, lipid metabolism in adipose tissue, adaptability to harsh environments, and evolutionary domestication. Through RNA-seq data analysis, we are able to compare the gene expression of fat-tailed sheep versus thin-tailed sheep breeds in an acceptable resolution at transcriptome level. The purpose of this study was to compare the transcriptomes of Ghezel (fat-tailed) and Zel (thin-tailed) sheep. Total RNA from subcutaneous and tail tissue samples from healthy lambs was sequenced (150b PE) to identify differentially expressed genes (DEGs) between the two mentioned tissues and between the Ghezel and Zel sheep breeds. Further downstream pathway and network analyses were conducted afterwards. The results uncovered the association of the most important DEGs such as CAV1, ALB, and SOCS3 with cellular signaling pathways of lipids metabolism. It seems that the SOCS3 gene plays an important role in the differential deposition of lipid in the tails of two phenotypically different sheep breeds. Although the detail of gene expression in the tail and subcutaneous tissues of two morphologically different breeds was decoded here, to fully understand how differential expression of the SOCS3 gene affects the fat synthesis, further studies are needed.

Today, feeding protein supply according to need in high-yielding lactating cows has become a big challenge. Protein is the most costly bulk constituent of animal diet, and the price of protein sources is increasing steadily, which is different from milk price rising. Therefore, one way for farmers to reduce feed costs is to reduce dietary protein share. Ruminants obtain their amino acids from 2 sources: amino acids from ruminally undegraded protein (RUP) and microbial protein synthesized in the rumen. A key goal in ruminant nutrition strategies, maximizing the use of rumen degradable protein (RDP), is through its efficient conversion into microbial protein. Urea is a supplement and a possible source of non-protein nitrogen (NPN) in ruminants' diets which meets bacteria's ammonia needs. Rumen ammonia sources include protein, peptides, amino acids, and other nitrogen-bearing compounds. As urea, uric acid, nitrate, and possibly nucleic acid are rapidly converted to ammonia, the ammonia reservoir indicates that the ruminal metabolism of ammonia is relatively small. Bacteria in the rumen can obtain between 40 and 95 percent of their nitrogen demand from ammonia, depending on their diet. Using NPN (non-protein nitrogen) as a reliable nitrogen source for ruminants was recognized over 100 years ago. Urea is quickly released in the rumen, its use in the diet is limited due to ammonia toxicity. So, the solution to this problem is that the product in nitrogen release rate from urea changes according to the digestion of fibers in the rumen. In the past, several slow-release products were made and evaluated. Slow-release urea (SRU) sources will also affect microbial growth and livestock performance compared to conventional plant protein sources. Acceptance of SRU sources, depending on their price compared to conventional plant protein ingredients is feasible. Studies has shown that the use of slow-release urea did not have a negative effect on digestibility, rumen parameters, milk production and livestock performance. Single-cell protein (SCP) is an emerging alternative protein source, currently being mainly studied for chicken and aquatic species.Finally, it is concluded that slow release urea can be used in feeding ruminants without any side effects.

The study aimed to explore the physicochemical attributes and the in-vitro digestibility of talipot starch lysine complexes generated through heat moisture treatment (HMT) and annealing. Talipot starch is a nonconventional stem starch obtained from the trunk of Talipot palm. Lysine complexing considerably altered the physicochemical, pasting, and in-vitro digestibility of talipot starch, while the impact of HMT and annealing on the physicochemical and functional properties of the starch lysine complex is varied. The HMT of the starch lysine complex (HSL) had a paramount impact on starch functionalities than annealing of starch lysine complex (ASL). The HSL exhibited the lowest swelling ability, solubility, and retrogradation tendency. Similarly, the lysin complexing of talipot starch considerably reduced its in-vitro digestibility, and HSL exhibited higher resistant starch content.

Study objectives included the assessment of carcass fatty acid composition and meat texture characteristics of younger Holstein Friesian bulls. Three experimental groups were formed based on the weights of the 23 young bulls at slaughter: lighter, medium, and heavier. Samples were taken from the Gluteus medius (GM) and Longissimus thoracis muscles 24 h after slaughter. Fatty acid composition, Warner-Bratzler Meat Shear (WBS) measurements, as well as textural profile analysis (TPA) and sensory analysis of the muscle samples were conducted. The fatty acid composition was determined using Thin Layer Chromatography (HPTLC). Polyunsaturated fatty acids and dietary fatty acids give a neutral hypocholesterolemic effect in direct fluorescent antibody (DFA) contents, DFA/OFA (C14:0+C16:0) ratio, hardness, Warner-Bratzler Shear force and also the chews number – which is desirable - before swallowing (NCBS) the meat were significantly decreased with the increasing slaughter weight. Higher slaughter weight resulted in a larger amount of beef with a better panel tenderness score; however, the meat obtained from the LSW group was less healthy considering the fatty acid profile. Additionally, internal fat contained the highest saturated fatty acids concentrations, while subcutaneous fat contained the highest amount of monounsaturated fatty acids. Furthermore, intramuscular fat levels were highest in PUFA and PUFA/SFA ratio. As a result, this study strongly suggests that slaughter weight and anatomical location of fat samples contribute significantly to meat texture characteristics and fatty acid profiles in Holstein Friesian bulls.

Utilizing passenger rail, including subways, to transport goods can have advantages over trucking in terms of efficiency and emissions. While some experimentation is ongoing in this area, combined passenger and rail opportunities in specific cities merit further attention. To more concretely examine how to leverage passenger rail for freight, this paper explores the potential of utilizing unused capacity in New York City (NYC) subway trains for transporting goods. Using General Transit Feed Specification (GTFS), rail and passenger data for the NYC subway, we found ways to use excess capacity and existing rail lines for freight transportation. We visualized a freight train timetable and graph, analyzed meet-errors between freight and passenger trains, and formulated prevention policies. Using U.S. Environmental Protection Agency data, we estimated emission reductions from replacing trucks with unused subway capacity. Our findings suggest that with adequate policies, investments and redesign, combining passenger rai and freight in the subways could significantly reduce truck trips, traffic congestion, and greenhouse gas emissions compared to trucking alone. However, implementing this approach requires careful collaborative planning, investment, enhanced security screening, and streamlined operations to minimize impacts on passenger transport. Further efforts should explore in more depth the costs and benefits and practical design and policy issues around using excess passenger rail capacity for freight transport in NYC and other cities.

Recently, plant proteins have received notable attention as food and medicine. Further, plant proteins are used to produce and isolate peptides to treat and prevent diseases in humans and animals. Plant proteins can be extracted from various sources, including industrial waste materials. Conventional and nonconventional methods are used to carry out the protein extraction. This review aims to overview the advanced protein extraction methods and the therapeutic functionalities of plant proteins and peptides. It was observed that the advanced protein extraction methods, i.e., ultrasound-assisted and microwave-assisted, are more efficient, eco-friendly, and less energy-consuming. The advanced protein extraction methods will help to face the challenges of using protein in food processing. In addition to the food functionality, plant-based proteins and peptides show potential therapeutic activity. It includes antidiabetic activity, anticancer activity, and anti-inflammatory activity. The pharmaceutical and nutraceutical industries will likely rely heavily on plant-derived peptides because of their benefits as functional ingredients.

In this study, three organic acids (citric, lactic, and acetic acid) were pretreated separately on P. rotundifolius starch, and their effects on the structural, functional, and rheological properties of cold water soluble (CWS) starches were investigated. In comparison to native starch, all the modified samples showed granular swelling, and acid pretreatment led to degradation and leaching of the granules. In FT-IR spectra, a peak at 1724 cm−1 confirmed the esterification of organic acids. All the modified starches lost their A-type diffraction pattern and showed a significant (p ≤ 0.05) decrease in the relative crystallinity. HPG showed a significantly higher cold water swelling power than native (2.13 g/g) starch, and lactic acid pretreatment enhanced the cold water swelling power to 9.91 g/g. Citric acid esterified CWS showed a significant increase in cold water solubility (63.1%) and freeze-thaw stability. Thus, this study found that each organic acid treatment affected CWS P. rotundifolius starch differently and has potential application in a refrigerated, instant, or frozen food products.