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Removal of cyanobacteria from the water column using a coagulant and a ballast compound is a promising technique to mitigate nuisance. As coagulant the organic, biodegradable polymer chitosan has been promoted. Results in this study show that elevated pH, as may be common during cyanobacterial blooms, as well as high alkalinity may hamper the coagulation of chitosan and thus impair its ability to effectively remove positively buoyant cyanobacteria from the water column. The underlying mechanism is likely a shielding of the protonated groups by anions. Inasmuch as there are many chitosan formulations, thorough testing of each chitosan prior to its application is essential. Results obtained in glass tubes were similar to those from standard jar tests demonstrating that glass tube tests can be used for testing effects of coagulants and ballasts in cyanobacteria removal whilst allowing far more replicates. There was no relation between zeta potential and precipitated cyanobacteria. Given the well-known antibacterial activity of chitosan and recent findings of anti-cyanobacterial effects, pre-application tests are needed to decipher if chitosan may cause cell leakage of cyanotoxins. Efficiency- and side-effect testing are crucial for water managers to determine if the selected approach can be used in tailor-made interventions to control cyanobacterial blooms and to mitigate eutrophication., (Copyright © 2017 Elsevier B.V. All rights reserved.)

We tested the hypothesis that a combination of coagulant and ballast could be efficient for removal of positively buoyant harmful cyanobacteria in shallow tropical waterbodies, and will not promote the release of cyanotoxins. This laboratory study examined the efficacy of coagulants [polyaluminium chloride (PAC) and chitosan (made of shrimp shells)] alone, and combined with ballast (lanthanum modified bentonite, red soil or gravel) to remove the natural populations of cyanobacteria collected from a shallow eutrophic urban reservoir with alternating blooms of Cylindrospermopsis and Microcystis. PAC combined with ballast was effective in settling blooms dominated by Microcystis or Cylindrospermopsis. Contrary to our expectation, chitosan combined with ballast was only effective in settling Cylindrospermopsis-dominated blooms at low pH, whereas at pH≥8 no effective flocculation and settling could be evoked. Chitosan also had a detrimental effect on Cylindrospermopsis causing the release of saxitoxins. In contrast, no detrimental effect on Microcystis was observed and all coagulant-ballast treatments were effective in not only settling the Microcystis dominated bloom, but also lowering dissolved microcystin concentrations. Our data show that the best procedure for biomass reduction also depends on the dominant species., (Copyright © 2017 Elsevier B.V. All rights reserved.)

Cyanobacteria have established dominant aquatic populations around the world, generally in aggressive environments and under severe stress conditions, e.g., intense solar radiation. Several marine strains make use of compounds such as the polyenic molecules for their damage protection justifying the range of colours observed for these species. The peridinin/chlorophyll-a/protein complex is an excellent example of essential structures used for self-prevention; their systems allow to them surviving under aggressive environments. In our simulations, few protective dyes are required to the initial specimen defense; this is an important data concern the synthetic priority in order to supply adequate damage protection. Raman measurements obtained with 1064 and 514.5 nm excitations for Cylindrospermopsis raciborskii and Microcystis aeruginosa strains shows bands assignable to the carotenoid peridinin. It was characterized by bands at 1940, 1650, 1515, 1449, 1185, 1155 and 1000 cm(-1) assigned to ν(C=C=C) (allenic vibration), ν(C=C/CO), ν(C=C), δ(C-H, C-18/19), δ(C-H), ν(C-C), and ρ(C-CH3), respectively. Recognition by Raman spectroscopy proved to be an important tool for preliminaries detections and characterization of polyene molecules in several algae, besides initiate an interesting discussion about their synthetic priority., (Copyright © 2015 Elsevier B.V. All rights reserved.)