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Every year, the production of rice straw and cow paunch content is abundant and 80 % of them has not been utilized. This study aimed to determine the potency of ensiling treatment on them in producing bioethanol. The novelty of this study is the utilization of cow paunch content as biomass and source of microbial fiber degradation. In this study, there were six groups of treatments. Bioethanol fermentation used Saccharomyces cerevisiae. Results, ensiling treatment significantly affected (p

Almost all farmers are unaware that rice straw and paunch manure are potential feedstocks for bioethanol besides organic fertilizer, biochar, and biogas. Farmers' perceptions regarding the multiattributes of bioethanol development are still underestimated, indicating that farmers are still indecisive about the potential use of rice straw and paunch manure as feedstock for bioethanol. They still need extension and training to improve their knowledge. The Location Quotient (LQ) value of rice straw and cattle paunch manure is > 1, indicating that Karanganyar Regency could become a base for bioethanol development from rice straw and cattle paunch manure.