18 results on '"Oulhen, Nathalie"'
Search Results
2. Trapping, tagging and tracking: Tools for the study of proteins during early development of the sea urchin
- Author
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Roux-Osovitz, Michelle M., primary, Foltz, Kathy R., additional, Oulhen, Nathalie, additional, and Wessel, Gary, additional
- Published
- 2019
- Full Text
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3. CRISPR/Cas9-mediated genome editing in sea urchins
- Author
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Lin, Che-Yi, primary, Oulhen, Nathalie, additional, Wessel, Gary, additional, and Su, Yi-Hsien, additional
- Published
- 2019
- Full Text
- View/download PDF
4. Methods to label, isolate, and image sea urchin small micromeres, the primordial germ cells (PGCs)
- Author
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Campanale, Joseph P., primary, Hamdoun, Amro, additional, Wessel, Gary M., additional, Su, Yi-Hsien, additional, and Oulhen, Nathalie, additional
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- 2019
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5. Identifying gene expression from single cells to single genes
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Oulhen, Nathalie, primary, Foster, Stephany, additional, Wray, Greg, additional, and Wessel, Gary, additional
- Published
- 2019
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6. Identification of the genes encoding candidate septate junction components expressed during early development of the sea urchin, Strongylocentrotus purpuratus, and evidence of a role for Mesh in the formation of the gut barrier.
- Author
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Jonusaite S, Oulhen N, Izumi Y, Furuse M, Yamamoto T, Sakamoto N, Wessel G, and Heyland A
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- Animals, Tight Junctions genetics, Tight Junctions metabolism, Epithelium metabolism, Intercellular Junctions metabolism, Drosophila metabolism, Sea Urchins genetics, Sea Urchins metabolism, Larva genetics, Larva metabolism, Strongylocentrotus purpuratus genetics, Strongylocentrotus purpuratus metabolism, Drosophila Proteins metabolism
- Abstract
Septate junctions (SJs) evolved as cell-cell junctions that regulate the paracellular barrier and integrity of epithelia in invertebrates. Multiple morphological variants of SJs exist specific to different epithelia and/or phyla but the biological significance of varied SJ morphology is unclear because the knowledge of the SJ associated proteins and their functions in non-insect invertebrates remains largely unknown. Here we report cell-specific expression of nine candidate SJ genes in the early life stages of the sea urchin Strongylocentrotus purpuratus. By use of in situ RNA hybridization and single cell RNA-seq we found that the expression of selected genes encoding putatively SJ associated transmembrane and cytoplasmic scaffold molecules was dynamically regulated during epithelial development in the embryos and larvae with different epithelia expressing different cohorts of SJ genes. We focused a functional analysis on SpMesh, a homolog of the Drosophila smooth SJ component Mesh, which was highly enriched in the endodermal epithelium of the mid- and hindgut. Functional perturbation of SpMesh by both CRISPR/Cas9 mutagenesis and vivo morpholino-mediated knockdown shows that loss of SpMesh does not disrupt the formation of the gut epithelium during gastrulation. However, loss of SpMesh resulted in a severely reduced gut-paracellular barrier as quantitated by increased permeability to 3-5 kDa FITC-dextran. Together, these studies provide a first look at the molecular SJ physiology during the development of a marine organism and suggest a shared role for Mesh-homologous proteins in forming an intestinal barrier in invertebrates. Results have implications for consideration of the traits underlying species-specific sensitivity of marine larvae to climate driven ocean change., Competing Interests: Declaration of competing interest None., (Copyright © 2022 Elsevier Inc. All rights reserved.)
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- 2023
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7. Gene regulatory divergence amongst echinoderms underlies appearance of pigment cells in sea urchin development.
- Author
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Spurrell M, Oulhen N, Foster S, Perillo M, and Wessel G
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- Animals, Starfish genetics, Transcription Factors metabolism, RNA, Gene Expression Regulation, Developmental genetics, Sea Urchins genetics, Sea Urchins metabolism
- Abstract
Larvae of the sea urchin, Strongylocentrotus purpuratus, have pigmented migratory cells implicated in immune defense and gut patterning. The transcription factor SpGcm activates the expression of many pigment cell-specific genes, including those involved in pigment biosynthesis (SpPks1 and SpFmo3) and immune related genes (e.g. SpMif5). Despite the importance of this cell type in sea urchins, pigmented cells are absent in larvae of the sea star, Patiria miniata. In this study, we tested the premises that sea stars lack genes to synthesize echinochrome pigment, that the genes are present but are not expressed in the larvae, or rather that the homologous gene expression does not contribute to echinochrome synthesis. Our results show that orthologs of sea urchin pigment cell-specific genes (PmPks1, PmFmo3-1 and PmMifL1-2) are present in the sea star genome and expressed in the larvae. Although no cell lineage homologous to migratory sea urchin pigment cells is present, dynamic gene activation accomplishes a similar spatial and temporal expression profile. The mechanisms regulating the expression of these genes, though, is highly divergent. In sea stars, PmGcm lacks the central role in pigment gene expression since it is not expressed in PmPks1 and PmFmo3-1-positive cells, and knockdown of Gcm does not abrogate pigment gene expression. Pigment genes are instead expressed in the coelomic mesoderm early in development before later being expressed in the ectoderm. These findings were supported by in situ RNA hybridization and comparative scRNA-seq analyses. We conclude that simply the coexpression of Pks1 and Fmo3 orthologs in cells of the sea star is not sufficient to underlie the emergence of the larval pigment cell in the sea urchin., Competing Interests: Declaration of competing interest The authors declare no competing interests., (Copyright © 2022 Elsevier Inc. All rights reserved.)
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- 2023
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8. Optimizing CRISPR/Cas9-based gene manipulation in echinoderms.
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Oulhen N, Pieplow C, Perillo M, Gregory P, and Wessel GM
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- Animals, Gene Editing methods, Morpholinos genetics, RNA, Guide, CRISPR-Cas Systems genetics, CRISPR-Cas Systems genetics, Sea Urchins genetics
- Abstract
The impact of new technology can be appreciated by how broadly it is used. Investigators that previously relied only on pharmacological approaches or the use of morpholino antisense oligonucleotide (MASO) technologies are now able to apply CRISPR-Cas9 to study biological problems in their model organism of choice much more effectively. The transitions to new CRISPR-based approaches could be enhanced, first, by standardized protocols and education in their applications. Here we summarize our results for optimizing the CRISPR-Cas9 technology in a sea urchin and a sea star, and provide advice on how to set up CRISPR-Cas9 experiments and interpret the results in echinoderms. Our goal through these protocols and sharing examples of success by other labs is to lower the activation barrier so that more laboratories can apply CRISPR-Cas9 technologies in these important animals., Competing Interests: Declaration of competing interest Synthego, IDT, and Aldevron each provided free samples of Cas9 and sgRNAs to initiate this project. All products thereafter were purchased at market value., (Copyright © 2022 Elsevier Inc. All rights reserved.)
- Published
- 2022
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9. A single-cell RNA-seq analysis of Brachyury-expressing cell clusters suggests a morphogenesis-associated signal center of oral ectoderm in sea urchin embryos.
- Author
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Satoh N, Hisata K, Foster S, Morita S, Nishitsuji K, Oulhen N, Tominaga H, and Wessel GM
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- Animals, Blastula metabolism, Ectoderm embryology, Endoderm embryology, Endoderm metabolism, Gastrula metabolism, Gene Regulatory Networks, Signal Transduction genetics, Ectoderm cytology, Ectoderm metabolism, Embryonic Development genetics, Fetal Proteins metabolism, Gene Expression Regulation, Developmental, RNA-Seq methods, Sea Urchins embryology, Sea Urchins genetics, Single-Cell Analysis methods, T-Box Domain Proteins metabolism
- Abstract
Brachyury is a T-box family transcription factor and plays pivotal roles in morphogenesis. In sea urchin embryos, Brachyury is expressed in the invaginating endoderm, and in the oral ectoderm of the invaginating mouth opening. The oral ectoderm is hypothesized to serve as a signaling center for oral (ventral)-aboral (dorsal) axis formation and to function as a ventral organizer. Our previous results of a single-cell RNA-seq (scRNA-seq) atlas of early Strongylocentrotus purpuratus embryos categorized the constituent cells into 22 clusters, in which the endoderm consists of three clusters and the oral ectoderm four clusters (Foster et al., 2020). Here we examined which clusters of cells expressed Brachyury in relation to the morphogenesis and the identity of the ventral organizer. Our results showed that cells of all three endoderm clusters expressed Brachyury in blastulae. Based on expression profiles of genes involved in the gene regulatory networks (GRNs) of sea urchin embryos, the three clusters are distinguishable, two likely derived from the Veg2 tier and one from the Veg1 tier. On the other hand, of the four oral-ectoderm clusters, cells of two clusters expressed Brachyury at the gastrula stage and genes that are responsible for the ventral organizer at the late blastula stage, but the other two clusters did not. At a single-cell level, most cells of the two oral-ectoderm clusters expressed organizer-related genes, nearly a half of which coincidently expressed Brachyury. This suggests that the ventral organizer contains Brachyury-positive cells which invaginate to form the stomodeum. This scRNA-seq study therefore highlights significant roles of Brachyury-expressing cells in body-plan formation of early sea urchin embryos, though cellular and molecular mechanisms for how Brachyury functions in these processes remain to be elucidated in future studies., Competing Interests: Declaration of competing interest The authors declare no competing or financial interests., (Copyright © 2022 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2022
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10. Post-transcriptional regulation of factors important for the germ line.
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Oulhen N, Morita S, and Wessel GM
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- Animals, Echinodermata metabolism, Germ Cells metabolism, Sea Urchins genetics, Embryo, Nonmammalian physiology, Gene Expression Regulation, Developmental
- Abstract
Echinoderms are a major model system for many general aspects of biology, including mechanisms of gene regulation. Analysis of transcriptional regulation (Gene regulatory networks, direct DNA-binding of proteins to specific cis-elements, and transgenesis) has contributed to our understanding of how an embryo works. This chapter looks at post-transcriptional gene regulation in the context of how the primordial germ cells are formed, and how the factors essential for this process are regulated. Important in echinoderms, as in many embryos, is that key steps of fate determination are made post-transcriptionally. This chapter highlights these steps uncovered in sea urchins and sea stars, and links them to a general theme of how the germ line may regulate its fate differently than many of the embryo's somatic cell lineages., (Copyright © 2022 Elsevier Inc. All rights reserved.)
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- 2022
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11. CRISPR-Cas9 editing of non-coding genomic loci as a means of controlling gene expression in the sea urchin.
- Author
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Pieplow A, Dastaw M, Sakuma T, Sakamoto N, Yamamoto T, Yajima M, Oulhen N, and Wessel GM
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- Animals, Animals, Genetically Modified, CRISPR-Associated Protein 9 genetics, Embryo, Nonmammalian metabolism, Embryonic Development genetics, Gene Expression, Gene Knockout Techniques, Germ Cells metabolism, Homeodomain Proteins genetics, Homeodomain Proteins metabolism, Nodal Protein genetics, Nodal Protein metabolism, RNA, Guide, CRISPR-Cas Systems genetics, RNA, Messenger genetics, RNA-Binding Proteins genetics, RNA-Binding Proteins metabolism, Transcription, Genetic genetics, CRISPR-Cas Systems, Gene Editing methods, Gene Expression Regulation, Developmental, Genetic Loci, Promoter Regions, Genetic genetics, Strongylocentrotus purpuratus embryology, Strongylocentrotus purpuratus genetics
- Abstract
We seek to manipulate gene function here through CRISPR-Cas9 editing of cis-regulatory sequences, rather than the more typical mutation of coding regions. This approach would minimize secondary effects of cellular responses to nonsense mediated decay pathways or to mutant protein products by premature stops. This strategy also allows for reducing gene activity in cases where a complete gene knockout would result in lethality, and it can be applied to the rapid identification of key regulatory sites essential for gene expression. We tested this strategy here with genes of known function as a proof of concept, and then applied it to examine the upstream genomic region of the germline gene Nanos2 in the sea urchin, Strongylocentrotus purpuratus. We first used CRISPR-Cas9 to target established genomic cis-regulatory regions of the skeletogenic cell transcription factor, Alx1, and the TGF-β signaling ligand, Nodal, which produce obvious developmental defects when altered in sea urchin embryos. Importantly, mutation of cis-activator sites (Alx1) and cis-repressor sites (Nodal) result in the predicted decreased and increased transcriptional output, respectively. Upon identification of efficient gRNAs by genomic mutations, we then used the same validated gRNAs to target a deadCas9-VP64 transcriptional activator to increase Nodal transcription directly. Finally, we paired these new methodologies with a more traditional, GFP reporter construct approach to further our understanding of the transcriptional regulation of Nanos2, a key gene required for germ cell identity in S. purpuratus. With a series of reporter assays, upstream Cas9-promoter targeted mutagenesis, coupled with qPCR and in situ RNA hybridization, we concluded that the promoter of Nanos2 drives strong mRNA expression in the sea urchin embryo, indicating that its primordial germ cell (PGC)-specific restriction may rely instead on post-transcriptional regulation. Overall, we present a proof-of-principle tool-kit of Cas9-mediated manipulations of promoter regions that should be applicable in most cells and embryos for which CRISPR-Cas9 is employed., (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Published
- 2021
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12. Distinct transcriptional regulation of Nanos2 in the germ line and soma by the Wnt and delta/notch pathways.
- Author
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Oulhen N, Swartz SZ, Wang L, Wikramanayake A, and Wessel GM
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- Animals, Germ Cells cytology, Germ Cells metabolism, Intracellular Signaling Peptides and Proteins metabolism, Membrane Proteins metabolism, Mesoderm cytology, Mesoderm embryology, Receptors, Notch metabolism, Strongylocentrotus purpuratus cytology, Gastrulation physiology, Gene Expression Regulation, Developmental physiology, RNA-Binding Proteins metabolism, Strongylocentrotus purpuratus embryology, Transcription, Genetic physiology, Wnt Signaling Pathway physiology
- Abstract
Specification of the primordial germ cells (PGCs) is essential for sexually reproducing animals. Although the mechanisms of PGC specification are diverse between organisms, the RNA binding protein Nanos is consistently required in the germ line in all species tested. How Nanos is selectively expressed in the germ line, however, remains largely elusive. We report that in sea urchin embryos, the early expression of Nanos2 in the PGCs requires the maternal Wnt pathway. During gastrulation, however, Nanos2 expression expands into adjacent somatic mesodermal cells and this secondary Nanos expression instead requires Delta/Notch signaling through the forkhead family member FoxY. Each of these transcriptional regulators were tested by chromatin immunoprecipitation analysis and found to directly interact with a DNA locus upstream of Nanos2. Given the conserved importance of Nanos in germ line specification, and the derived character of the micromeres and small micromeres in the sea urchin, we propose that the ancestral mechanism of Nanos2 expression in echinoderms was by induction in mesodermal cells during gastrulation., (Copyright © 2019 Elsevier Inc. All rights reserved.)
- Published
- 2019
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13. A quiet space during rush hour: Quiescence in primordial germ cells.
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Oulhen N and Wessel G
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- Animals, Caenorhabditis elegans cytology, Cell Differentiation, Cell Division, Drosophila cytology, Mice, Germ Cells cytology, Hematopoietic Stem Cells cytology
- Abstract
Quiescence is a common character in stem cells. Low cellular activity in these cells may function to minimize the potential damaging effects of oxidative stress, reduce the number of cells needed for tissue replenishment, and as a consequence, perhaps occupy unique niches. Quiescent stem cells are found in many adult human tissues, the hematopoietic stem cells are paradigmatic, and more recently it appears that stem cell of the germ line in many animals display quiescence characters. Here we explore the diversity of quiescence phenotypes in primordial germ cells, leveraging the diverse mechanisms of germ cell formation to extract evolutionary significance to common processes., (Copyright © 2017 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2017
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14. Regeneration in bipinnaria larvae of the bat star Patiria miniata induces rapid and broad new gene expression.
- Author
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Oulhen N, Heyland A, Carrier TJ, Zazueta-Novoa V, Fresques T, Laird J, Onorato TM, Janies D, and Wessel G
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- Animals, Cell Proliferation genetics, Gene Expression Regulation, Developmental genetics, Larva genetics, Starfish genetics, Larva growth & development, Protein Biosynthesis genetics, Regeneration genetics, Starfish growth & development
- Abstract
Background: Some metazoa have the capacity to regenerate lost body parts. This phenomenon in adults has been classically described in echinoderms, especially in sea stars (Asteroidea). Sea star bipinnaria larvae can also rapidly and effectively regenerate a complete larva after surgical bisection. Understanding the capacity to reverse cell fates in the larva is important from both a developmental and biomedical perspective; yet, the mechanisms underlying regeneration in echinoderms are poorly understood., Results: Here, we describe the process of bipinnaria regeneration after bisection in the bat star Patiria miniata. We tested transcriptional, translational, and cell proliferation activity after bisection in anterior and posterior bipinnaria halves as well as expression of SRAP, reported as a sea star regeneration associated protease (Vickery et al., 2001b). Moreover, we found several genes whose transcripts increased in abundance following bisection, including: Vasa, dysferlin, vitellogenin 1 and vitellogenin 2., Conclusion: These results show a transformation following bisection, especially in the anterior halves, of cell fate reassignment in all three germ layers, with clear and predictable changes. These results define molecular events that accompany the cell fate changes coincident to the regenerative response in echinoderm larvae., (Crown Copyright © 2016. Published by Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2016
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15. Differential Nanos 2 protein stability results in selective germ cell accumulation in the sea urchin.
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Oulhen N and Wessel GM
- Subjects
- Animals, Gene Expression Regulation, Developmental, Gene Knockout Techniques, Morpholinos, Protein Biosynthesis genetics, Protein Stability, RNA, Messenger biosynthesis, Starfish embryology, Sumoylation, Ubiquitination, Germ Cells metabolism, Open Reading Frames genetics, RNA-Binding Proteins genetics, RNA-Binding Proteins metabolism, Strongylocentrotus purpuratus embryology
- Abstract
Nanos is a translational regulator required for the survival and maintenance of primordial germ cells. In the sea urchin, Strongylocentrotus purpuratus (Sp), Nanos 2 mRNA is broadly transcribed but accumulates specifically in the small micromere (sMic) lineage, in part because of the 3'UTR element GNARLE leads to turnover in somatic cells but retention in the sMics. Here we found that the Nanos 2 protein is also selectively stabilized; it is initially translated throughout the embryo but turned over in the future somatic cells and retained only in the sMics, the future germ line in this animal. This differential stability of Nanos protein is dependent on the open reading frame (ORF), and is independent of the sumoylation and ubiquitylation pathways. Manipulation of the ORF indicates that 68 amino acids in the N terminus of the Nanos protein are essential for its stability in the sMics whereas a 45 amino acid element adjacent to the zinc fingers targets its degradation. Further, this regulation of Nanos protein is cell autonomous, following formation of the germ line. These results are paradigmatic for the unique presence of Nanos in the germ line by a combination of selective RNA retention, distinctive translational control mechanisms (Oulhen et al., 2013), and now also by defined Nanos protein stability., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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16. Conservation of sequence and function in fertilization of the cortical granule serine protease in echinoderms.
- Author
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Oulhen N, Xu D, and Wessel GM
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- Amino Acid Sequence, Animals, Conserved Sequence, Echinodermata classification, Echinodermata genetics, Female, Fertilization drug effects, Fertilization genetics, Male, Molecular Sequence Data, Ovum drug effects, Ovum physiology, Phylogeny, Sequence Homology, Amino Acid, Serine Proteases genetics, Serine Proteinase Inhibitors pharmacology, Sperm-Ovum Interactions drug effects, Sperm-Ovum Interactions genetics, Sperm-Ovum Interactions physiology, Strongylocentrotus purpuratus drug effects, Strongylocentrotus purpuratus genetics, Strongylocentrotus purpuratus physiology, Transcriptome, Echinodermata physiology, Fertilization physiology, Serine Proteases physiology
- Abstract
Conservation of the cortical granule serine protease during fertilization in echinoderms was tested both functionally in sea stars, and computationally throughout the echinoderm phylum. We find that the inhibitor of serine protease (soybean trypsin inhibitor) effectively blocks proper transition of the sea star fertilization envelope into a protective sperm repellent, whereas inhibitors of the other main types of proteases had no effect. Scanning the transcriptomes of 15 different echinoderm ovaries revealed sequences of high conservation to the originally identified sea urchin cortical serine protease, CGSP1. These conserved sequences contained the catalytic triad necessary for enzymatic activity, and the tandemly repeated LDLr-like repeats. We conclude that the protease involved in the slow block to polyspermy is an essential and conserved element of fertilization in echinoderms, and may provide an important reagent for identification and testing of the cell surface proteins in eggs necessary for sperm binding., (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Published
- 2014
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17. Dysferlin is essential for endocytosis in the sea star oocyte.
- Author
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Oulhen N, Onorato TM, Ramos I, and Wessel GM
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- Animals, Calcium chemistry, Calcium-Binding Proteins metabolism, Cell Membrane metabolism, Gastrula metabolism, Humans, Mass Spectrometry, Membrane Proteins metabolism, Microscopy, Fluorescence, Protein Binding, Protein Structure, Tertiary, Proteomics, Rhodamines chemistry, Starfish, Calcium-Binding Proteins physiology, Endocytosis genetics, Gene Expression Regulation, Developmental, Membrane Proteins physiology, Oocytes cytology
- Abstract
Dysferlin is a calcium-binding transmembrane protein involved in membrane fusion and membrane repair. In humans, mutations in the dysferlin gene are associated with muscular dystrophy. In this study, we isolated plasma membrane-enriched fractions from full-grown immature oocytes of the sea star, and identified dysferlin by mass spectrometry analysis. The full-length dysferlin sequence is highly conserved between human and the sea star. We learned that in the sea star Patiria miniata, dysferlin RNA and protein are expressed from oogenesis to gastrulation. Interestingly, the protein is highly enriched in the plasma membrane of oocytes. Injection of a morpholino against dysferlin leads to a decrease of endocytosis in oocytes, and to a developmental arrest during gastrulation. These results suggest that dysferlin is critical for normal endocytosis during oogenesis and for embryogenesis in the sea star and that this animal may be a useful model for studying the relationship of dysferlin structure as it relates to its function., (Copyright © 2014 Elsevier Inc. All rights reserved.)
- Published
- 2014
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18. The 3'UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin.
- Author
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Oulhen N, Yoshida T, Yajima M, Song JL, Sakuma T, Sakamoto N, Yamamoto T, and Wessel GM
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- Animals, Base Pairing genetics, Base Sequence, Carrier Proteins metabolism, Conserved Sequence genetics, Genes, Reporter, Molecular Sequence Data, Nucleotides genetics, Protein Biosynthesis genetics, Regulatory Sequences, Ribonucleic Acid genetics, Sequence Deletion, 3' Untranslated Regions genetics, Carrier Proteins genetics, Cell Lineage genetics, Germ Cells cytology, Hemicentrotus cytology, Hemicentrotus genetics, Strongylocentrotus purpuratus cytology, Strongylocentrotus purpuratus genetics
- Abstract
Nanos is a translational regulator required for the survival and maintenance of primordial germ cells during embryogenesis. Three nanos homologs are present in the genome of the sea urchin Strongylocentrotus purpuratus (Sp), and each nanos mRNA accumulates specifically in the small micromere (sMic) lineage. We found that a highly conserved element in the 3' UTR of nanos2 is sufficient for reporter expression selectively in the sMic lineage: microinjection into a Sp fertilized egg of an RNA that contains the GFP open reading frame followed by Sp nanos2 3'UTR leads to selective reporter enrichment in the small micromeres in blastulae. The same result was seen with nanos2 from the sea urchin Hemicentrotus pulcherrimus (Hp). In both species, the 5'UTR alone is not sufficient for the sMic localization but it always increased the sMic reporter enrichment when present with the 3'UTR. We defined an element conserved between Hp and Sp in the nanos2 3'UTR which is necessary and sufficient for protein enrichment in the sMic, and refer to it as GNARLE (Global Nanos Associated RNA Lability Element). We also found that the nanos2 3'UTR is essential for the selective RNA retention in the small micromeres; GNARLE is required but not sufficient for this process. These results show that a combination of selective RNA retention and translational control mechanisms instills nanos accumulation uniquely in the sMic lineage., (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Published
- 2013
- Full Text
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