1. The presence of free palmitic acid modulates the effects of lutein on structural and dynamic properties of lipid membranes.
- Author
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Reszczyńska E, Wiśniewska-Becker A, Duda M, Sęk A, Gruszecki WI, and Hanaka A
- Subjects
- Humans, Electron Spin Resonance Spectroscopy, Palmitic Acids, Lipids, Lipid Bilayers chemistry, Dimyristoylphosphatidylcholine chemistry, Lutein pharmacology, Lutein chemistry, Xanthophylls
- Abstract
Free fatty acids, like palmitic acid (PA), and xanthophyll pigments, like lutein (LUT) are the natural membrane compounds in plants. To study the effect of PA on LUT and their organization, a model membrane of 1,2-dimyristoyl-sn-glycerol-3-phosphocholine (DMPC) enriched with 2 mol% PA and 1 mol% LUT was formed. Molecular mechanisms underlying the interaction between these two compounds were examined with application of molecular spectroscopy techniques, e.g., visible spectroscopy, electron paramagnetic resonance and Fourier transform infrared. We determined the monomeric/dimeric organization of LUT in the membrane. We proved that the presence of PA in the lipid phase facilitated and stabilized the formation of LUT structures in the membrane. Lutein with PA did not form strong molecular aggregates like H- and J-structures. We presented the simplified model membrane that could be a suitable representation of the physiological process of de-esterification of PA from LUT appearing in natural biomembranes in humans., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Inc. All rights reserved.) more...
- Published
- 2024
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