16 results on '"Pandey, Lalit M."'
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2. Plant-polyphenol-mediated synthesis of iron oxide nanomaterials for heavy metal removal
- Author
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Jawed, Aquib, primary, Sharma, Swati, additional, Golder, Animes K., additional, and Pandey, Lalit M., additional
- Published
- 2021
- Full Text
- View/download PDF
3. Contributors
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Agrawal, Komal, primary, Anandkumar, J., additional, Banerjee, Aditi, additional, Bergi, Jay, additional, Bharadvaja, Navneeta, additional, Cai, Q.Q., additional, Das, Jayashankar, additional, Datta, Shivika, additional, Dave, Sushma, additional, Deng, S.H., additional, Dhanjal, Daljeet Singh, additional, Garg, Anurag, additional, Ghosh Sachan, Shashwati, additional, Hu, J.Y., additional, Jayasree, Pyla, additional, Jothinathan, L., additional, Kaur, Sukhmanpreet, additional, Khare, Prateek, additional, Kumar, Prashant, additional, Kumar, Vijay, additional, Kumar, Vineet, additional, Kundu, Lal Mohan, additional, Mishra, Sandhya, additional, Ng, H.Y., additional, Ong, S.L., additional, Pandey, Lalit M., additional, Patel, Ratnesh Kumar, additional, Prashanth, V., additional, Rajput, Parth, additional, Ram, Shristi, additional, Remya, Neelancherry, additional, Romero, Romina, additional, Roy, Arpita, additional, Sahariah, Biju Prava, additional, Satapathy, Monalisa, additional, Shah, Maulin P., additional, Shankar, Ravi, additional, Sharan, Shambhoo, additional, Singh, Joginder, additional, Singh, Kshitij, additional, Singh, Simranjeet, additional, Singh, Swati, additional, Tirkey, Sushma Rani, additional, Trivedi, Ratna, additional, Verma, Ayushi, additional, Verma, Pradeep, additional, Verma, Rahul, additional, and Yadu, Aparna, additional
- Published
- 2021
- Full Text
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4. Decontamination of distillery spent wash through advanced oxidation methods
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Verma, Rahul, primary, Kundu, Lal Mohan, additional, and Pandey, Lalit M., additional
- Published
- 2021
- Full Text
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5. Nano-sorbents-assisted microbial bioremediation of hazardous petroleum hydrocarbons
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Sharma, Swati, primary and Pandey, Lalit M., additional
- Published
- 2020
- Full Text
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6. Hydroxyapatite: an inorganic ceramic for biomedical applications
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Saxena, Varun, primary, Shukla, Ishani, additional, and Pandey, Lalit M., additional
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- 2019
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7. List of Contributors
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Aggarwal, Geeta, primary, Ahmad, Mudasir, additional, Ahmad, Suhail, additional, Amorim, M. Teresa P., additional, Andronescu, Ecaterina, additional, Barreiro, Maria Filomena, additional, Batra, Sonali, additional, Brar, Amandeep, additional, Calabrese, L., additional, Campos-Cruz, Juan-Ramòn, additional, Chaturvedi, Thakur Prasad, additional, Costache, Marieta, additional, Dias, Madalena Maria, additional, Fabiano, F., additional, Felgueiras, Helena P., additional, Ficai, Anton, additional, Galateanu, Bianca, additional, Grumezescu, Alexandru Mihai, additional, Han, Sung Soo, additional, Hudita, Ariana, additional, Ikram, Saiqa, additional, Kumar, Anuj, additional, Kumar, Ashish, additional, Kumar, Manish, additional, Lopes, Jose Carlos, additional, Maman, Paramjot, additional, Manzoor, Kaiser, additional, Nagpal, Manju, additional, Oprea, Alexandra Elena, additional, Pandey, Lalit M., additional, Pareek, Nidhi, additional, Popescu, Roxana C., additional, Prakash, Rajiv, additional, Proverbio, E., additional, Rangel-Vàzquez, Norma-Aurea, additional, Rangel-Vàzquez, Ricardo, additional, Ruphuy, Gabriela, additional, Saharan, Lokesh, additional, Saxena, Varun, additional, Sharma, Sumit, additional, Shukla, Ishani, additional, Tadesse, Yonas, additional, Vivek, Rajul, additional, and Vivekanand, V., additional
- Published
- 2019
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8. Unfolding of the SARS-CoV-2 spike protein through infrared and ultraviolet-C radiation based disinfection.
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Mahanta N, Sharma S, Sharma LG, Pandey LM, and Dixit US
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- Humans, Disinfection methods, SARS-CoV-2, Staphylococcus aureus, Respiratory Aerosols and Droplets, Ultraviolet Rays, Spike Glycoprotein, Coronavirus, COVID-19
- Abstract
The spreading of coronavirus from contacting surfaces and aerosols created a pandemic around the world. To prevent the transmission of SARS-CoV-2 virus and other contagious microbes, disinfection of contacting surfaces is necessary. In this study, a disinfection box equipped with infrared (IR) radiation heating and ultraviolet-C (UV-C) radiation is designed and tested for its disinfection ability against pathogenic bacteria and SARS-CoV-2 spike protein. The killing of a Gram-positive, namely, S. aureus and a Gram-negative namely, S. typhi bacteria was studied followed by the inactivation of the spike protein. The experimental parameters were optimized using a statistical tool. For the broad-spectrum antibacterial activity, the optimum condition was holding at 65.61 °C for 13.54 min. The killing of the bacterial pathogen occurred via rupturing the cell walls as depicted by electron microscopy. Further, the unfolding of SARS-CoV-2 spike protein and RNase A was studied under IR and UV-C irradiations at the aforesaid optimized condition. The unfolding of both the proteins was confirmed by changes in the secondary structure, particularly an increase in β-sheets and a decrease in α-helixes. Remarkably, the higher penetration depth of IR waves up to subcutaneous tissue resulted in lower optimum disinfection temperature, <70 °C in vogue. Thus, the combined UV-C and IR radiation is effective in killing the pathogenic bacteria and denaturing the glycoproteins., Competing Interests: Declaration of competing interest The authors declare that there is no conflict of interest., (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Published
- 2022
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9. Performance study of a sterilization box using a combination of heat and ultraviolet light irradiation for the prevention of COVID-19.
- Author
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Mahanta N, Saxena V, Pandey LM, Batra P, and Dixit US
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- Hot Temperature, Humans, SARS-CoV-2, Virus Inactivation, COVID-19, Ultraviolet Rays
- Abstract
SARS-CoV-2 virus and other pathogenic microbes are transmitted to the environment through contacting surfaces, which need to be sterilized for the prevention of COVID-19 and related diseases. In this study, a prototype of a cost-effective sterilization box is developed to disinfect small items. The box utilizes ultra violet (UV) radiation with heat. For performance assessment, two studies were performed. First, IgG (glycoprotein, a model protein similar to that of spike glycoprotein of SARS-COV-2) was incubated under UV and heat sterilization. An incubation with UV at 70 °C for 15 min was found to be effective in unfolding and aggregation of the protein. At optimized condition, the hydrodynamic size of the protein increased to ~171 nm from ~5 nm of the native protein. Similarly, the OD
280 values also increased from 0.17 to 0.78 indicating the exposure of more aromatic moieties and unfolding of the protein. The unfolding and aggregation of the protein were further confirmed by the intrinsic fluorescence measurement and FTIR studies, showing a 70% increase in the β-sheets and a 22% decrease in the α-helixes of the protein. The designed box was effective in damaging the protein's native structure indicating the effective inactivation of the SARS-COV-2. Furthermore, the incubation at 70 °C for 15 min inside the chamber resulted in 100% antibacterial efficacy for the clinically relevant E.coli bacteria as well as for bacteria collected from daily use items. It is the first detailed performance study on the efficacy of using UV irradiation and heat together for disinfection from virus and bacteria., (Copyright © 2021 Elsevier Inc. All rights reserved.)- Published
- 2021
- Full Text
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10. Yttrium iron garnet for hyperthermia applications: Synthesis, characterization and in-vitro analysis.
- Author
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Fopase R, Saxena V, Seal P, Borah JP, and Pandey LM
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- Humans, Hyperthermia, Magnetics, Particle Size, Iron, Yttrium
- Abstract
Exclusive magnetocaloric properties of orthoferrites offer advantages for their application in the magnetic hyperthermia as well as imaging applications. In the present study, the effect of yttrium concentration on the magnetic characteristics of the iron oxide based nanomaterials was analyzed to assess their potential for the hyperthermia applications. The Sol-gel method was used to synthesize the Yttrium Iron Garnet (YIG) based nanoparticles, using different molar ratios of Fe and Y precursors, followed by the calcination at 900, 1000 and 1100 °C. XRD analysis determined the formation of the pure phase of yttrium iron garnet Y
3 Fe5 O12 (YIG) at 0.5 molar ratio of yttrium at all the calcination temperatures and pure phase of yttrium iron perovskite YFeO3 (YIP) for 1 molar ratio of yttrium at 1000 and 1100 °C. The mean particle size was observed in the range of 100 to 400 nm. The magnetic characterization studies showed the highest saturation magnetization for the sample containing 0.5 molar ratio of the yttrium calcinated at 1000 °C. The magnetization values were linearly related to the contents of YIG phases in the synthesized samples. Induction heating of YIG resulted in the hyperthermia temperature (42 to 44 °C) in 13 min with the SAR values 114.65 W/g at 1 mg/ml. The prepared samples showed no in-vitro toxic effects on the MG63 cells (>90% cell viability). In addition, in-vitro treatment at hyperthermia temperature for 15 min reduced cell viability of cancer cells (A549) to 55%, while no toxic effect was observed on MG 63 cells. The present study postulates Yttrium Iron Garnet as an effective therapeutic agent for hyperthermia cancer treatment., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
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11. Proline functionalized gold nanoparticles modulates lysozyme fibrillation.
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Karmakar S, Sarkar N, and Pandey LM
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- Animals, Metal Nanoparticles chemistry, Muramidase drug effects, Gold chemistry, Metal Nanoparticles administration & dosage, Muramidase chemistry, Proline chemistry, Protein Aggregates drug effects
- Abstract
Amyloid fibrils are the hallmarks of neurodegenerative diseases like Alzheimer's, Parkinson's and other proteopathies. Inhibition of fibrillation is a potential strategy to check the progress of amyloid associated diseases and further allied deterioration. In this study, we have synthesized proline functionalized gold nanoparticles (Pro-AuNPs) and scrutinized its antifibrillation property towards Hen Egg White Lysozyme (HEWL) aggregation. Pro-AuNPs were characterized using various biophysical methods like ultraviolet-visible spectroscopy, fourier transform infra-red spectroscopy, zeta potential measurement, dynamic light scattering and transmission electron microscopy. The effect of Pro-AuNPs on HEWL fibrillation was analyzed employing thioflavin T (ThT) and 8-Anilino-1-naphthalenesulfonic acid (ANS) assays. The kinetics of HEWL exhibited a typical sigmoidal nature of protein aggregation and was fitted to Boltzmann model. HEWL in the presence of bare gold nanoparticles (bAuNPs) exhibited similar aggregation kinetics as HEWL alone. However, HEWL fibrillation substantially reduced upon co-incubation with proline and Pro-AuNPs, and two slightly different intermediate species were formed with these two systems as predicted by CD spectroscopy. TEM images also supported the above observation displaying different morphological states of HEWL aggregates in the presence of proline and Pro-AuNPs. Using computational methods, the nature of interaction of HEWL and proline was found to be hydrogen bonding and hydrophobic interaction in multiple amyloidogenic regions. These interactions inhibited the formation of prefibrils (β-sheet rich intermediates) and also found to disintegrate fibrils. Furthermore, HEWL-Pro-AuNPs system resulted HEWL adsorption through hydrophobic patches, which blocked the intermolecular β-sheet formation. The present study successfully established Pro-AuNPs as a potential inhibitor of HEWL aggregation., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2019
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12. Synthesis, characterization and in vitro analysis of α-Fe 2 O 3 -GdFeO 3 biphasic materials as therapeutic agent for magnetic hyperthermia applications.
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Deka S, Saxena V, Hasan A, Chandra P, and Pandey LM
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- Magnetic Fields, Magnetic Resonance Imaging, Ferric Compounds chemistry, Gadolinium chemistry, Hyperthermia, Induced methods
- Abstract
The use of gadolinium orthoferrite for biomedical application like as contrast agents for magnetic resonance imaging (MRI) has been found to be very promising due to its fascinating properties. The present study focuses on the determination of the effect of the gadolinium concentration in the formation biphasic α-Fe
2 O3 -GdFeO3 for hyperthermia applications. An in-situ sol-gel technique was adopted for the synthesis of biphasic orthoferrites with four different gadolinium concentrations. The XRD analysis confirmed the formation of gadolinium orthoferrites after heat treatment at 1000 °C, 1100 °C, and 1200 °C. The presence of α-Fe2 O3 in trace amounts was observed in the materials with low gadolinium concentrations. VSM (Vibrating-sample magnetometer) analysis was performed to ensure the magnetic properties of the materials, which were found to be weakly ferromagnetic. The biocompatibility of the materials was investigated through MTT assay and no cytotoxic effect was observed. The assessment of heating ability of the materials was performed under an alternating magnetic field using an induction heating instrument and all the samples showed temperature rise in the range of hyperthermia temperature with a maximum temperature of 55.71 °C in 6 min. The heating experiments at 44 °C in the absence of samples established the vulnerability of cancer cells as compared to normal cells., (Copyright © 2018 Elsevier B.V. All rights reserved.)- Published
- 2018
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13. Nano-biocomposite scaffolds of chitosan, carboxymethyl cellulose and silver nanoparticle modified cellulose nanowhiskers for bone tissue engineering applications.
- Author
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Hasan A, Waibhaw G, Saxena V, and Pandey LM
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- Biocompatible Materials chemistry, Biocompatible Materials therapeutic use, Bone and Bones drug effects, Carboxymethylcellulose Sodium therapeutic use, Chitosan therapeutic use, Humans, Metal Nanoparticles therapeutic use, Osteoblasts drug effects, Porosity, Silver chemistry, Tissue Scaffolds chemistry, Carboxymethylcellulose Sodium chemistry, Chitosan chemistry, Metal Nanoparticles chemistry, Tissue Engineering
- Abstract
In the present work, we aimed to synthesize highly efficient nano-composite polymeric scaffolds with controllable pore size and mechanical strength. We prepared nanocomposite (CCNWs-AgNPs) of silver nanoparticles (AgNPs) decorated on carboxylated CNWs (CCNWs) which serves dual functions of providing mechanical strength and antimicrobial activity. Scaffolds containing chitosan (CS) and carboxymethyl cellulose (CMC) with varying percent of nanocomposite were fabricated using freeze drying method. XRD and FESEM analysis of nanocomposite revealed highly crystalline structure with AgNPs (5.2 nm dia) decorated on ~200 nm long CCNWs surface. FTIR analysis confirmed the interaction between CCNWs and AgNPs. Incorporation of nanocomposite during scaffolds preparation helped in achieving the desirable 80-90% porosity with pore diameter ranging between 150 and 500 μm and mechanical strength was also significantly improved matching with the mechanical strength of cancellous bone. The swelling capacity of scaffolds decreased after the incorporation of nanocomposite. In turn, scaffold degradation rate was tuned to support angiogenesis and vascularization. Scaffolds apart from exhibiting excellent antimicrobial activity, also supported MG63 cells adhesion and proliferation. Incorporation of CCNWs also resulted in improved biomineralization for bone growth. Overall, these studies confirmed excellent properties of fabricated scaffolds, making them self-sustained and potential antimicrobial scaffolds (without any loaded drug) to overcome bone related infections like osteomyelitis., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
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14. Deciphering the mechanistic insight into the stoichiometric ratio dependent behavior of Cu(II) on BSA fibrillation.
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Singh A, Datta P, and Pandey LM
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- Animals, Binding Sites, Cattle, Copper metabolism, Hydrogen-Ion Concentration, Kinetics, Models, Molecular, Protein Structure, Secondary drug effects, Serum Albumin, Bovine metabolism, Thermodynamics, Copper pharmacology, Protein Aggregates, Serum Albumin, Bovine chemistry
- Abstract
Various metal ions are recently implicated in protein aggregates and are associated with numerous neurodegenerative diseases. In the present work, we have scrutinized the effect of stoichiometric variation of Cu(II) on BSA fibrillation at physiological pH 7.4 through Thioflavin-T dye binding study, residual protein concentration, Fourier Transform Infrared spectroscopy (FTIR), Dynamic Light Scattering (DLS), Atomic Force Microscopy (AFM) and Isothermal Titration Calorimetry (ITC). Fibrillation kinetics was studied through ThT fluorescence, which illustrated dependency on stoichiometric ratio of Cu(II). The ThT intensity data were fitted to a single exponential expression to determine the aggregation rate, k, which revealed a slight lower k value for 1:1 ratio of BSA-Cu(II) reaction as compared to BSA alone whereas k value for 1:2 ratio of BSA-Cu(II) reaction was higher. Higher equilibrium residual concentration in case of 1:1 ratio of BSA-Cu(II) agreed to the lower aggregation rate. IR spectroscopy revealed the presence of increased β-sheet proportion to the detriment of α-helix conformation with increasing concentration of Cu(II) and illustrated maximum β-sheet proportion in 1:2 ratio of BSA-Cu(II). These results were combined with scattering results that showed the higher average hydrodynamic radius (Z-average value) of aggregates in 1:2 ratio of BSA-Cu(II) with respect to BSA and 1:1 ratio of BSA-Cu(II). AFM analysis confirmed the fibril formation. ITC analysis has shown the presence of two binding sites with many fold difference in binding affinity at prescribed in vitro conditions. An N-terminal binding site with many fold higher binding affinity was found as the first Cu(II) binding site. The free thiol group of the cysteine residue positioned at 34 (cys-34) in BSA was covalently capped and this modified BSA also showed the presence of two binding sites, which declined the cys-34 site as the second Cu(II) bind site. Therefore Zn(II) binding site was predicted as the second Cu(II) binding site. The binding of Cu(II) at N-terminal binding site marks a square planner geometry; while that at zinc binding site marks a rigid coordination geometry. Conclusively, analyzed data has concluded that the variation in Cu(II) efficacy in the different stoichiometric ratio results because of two different coordination types in different stoichiometric ratios., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2017
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15. Kinetic studies of attachment and re-orientation of octyltriethoxysilane for formation of self-assembled monolayer on a silica substrate.
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Hasan A and Pandey LM
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- Surface Properties, Silanes chemistry, Silicon Dioxide chemistry
- Abstract
The present study deals with kinetic studies of the chemical modification for synthesizing a hydrophobic silica surface. Surface silanization (modification) via formation of Self-Assembled Monolayer (SAM) using a short chain triethoxyoctylsilane (TEOS) was carried out under inert atmosphere at room temperature. Fourier transmission infrared (FTIR) spectroscopy, water contact angle (WCA) and atomic force microscopy (AFM) were employed to investigate surface modification. FTIR analysis in the range from 900-1200cm(-1) and 2850-3000cm(-1) confirmed surface modification and re-orientation of the attached molecules. Kinetic studies of TEOS SAM formation were fitted by Exponential Association function. Kinetic fitting of FTIR data in the range from 900-1200cm(-1) revealed a very fast attachment of TEOS molecules resulting in total surface coverage within 16min whereas re-orientation rate was slow and continued till 512min. Further, change in orientation from lying-down to standing-up state was supported by contact angle analysis. AFM images initially showed small islands of ~20nm, which in-fill with time indicating formation of a smooth monolayer. Our findings indicate that formation of octyl SAM is fast process and completes within 8.5h in contrary to reported 24h in conventional SAM formation protocols. The kinetic fitting data can be explored to design a nanopatterned surface for a specific application., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
- Full Text
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16. Surface chemistry at the nanometer scale influences insulin aggregation.
- Author
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Pandey LM, Le Denmat S, Delabouglise D, Bruckert F, Pattanayek SK, and Weidenhaupt M
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- Adsorption, Benzothiazoles, Fluorescent Dyes, Glass chemistry, Humans, Hydrophobic and Hydrophilic Interactions, Kinetics, Microscopy, Atomic Force, Nanostructures ultrastructure, Protein Structure, Secondary, Recombinant Proteins chemistry, Spectrometry, Fluorescence, Spectroscopy, Fourier Transform Infrared, Surface Properties, Thiazoles, Coated Materials, Biocompatible chemical synthesis, Insulin chemistry, Nanostructures chemistry, Silanes chemistry
- Abstract
We synthesized surfaces with different hydrophobicities and roughness by forming self-assembled monolayers (SAMs) of mixed amine and octyl silanes. Insulin aggregation kinetics in the presence of the above surfaces is characterized by a typical lag phase and growth rate. We show that the lag time but not the growth rate varies as a function of the amine fraction on the surface. The amount of adsorbed protein and the adsorption rate during the aggregation process also vary with the amine fraction on the surface and are maximal for equal parts of amine and octyl groups. For all surfaces, the growth phase starts for identical amounts of adsorbed insulin. The initial surface roughness determines the rate at which protein adsorption occurs and hence the time to accumulate enough protein to form aggregation nuclei. In addition, the surface chemistry and topography influence the morphology of aggregates adsorbed on the material surface and the secondary structures of final aggregates released in solution., (Copyright © 2012 Elsevier B.V. All rights reserved.)
- Published
- 2012
- Full Text
- View/download PDF
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