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Your search keyword '"Paul D. Fey"' showing total 6 results
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6 results on '"Paul D. Fey"'

1. Staphylococcus aureus skin colonization is mediated by SasG lectin variation

Author

Krista B. Mills, Joseph J. Maciag, Can Wang, John A. Crawford, Timothy J. Enroth, Klara C. Keim, Yves F. Dufrêne, D. Ashley Robinson, Paul D. Fey, Andrew B. Herr, and Alexander R. Horswill

Subjects
CP: Microbiology, Biology (General), QH301-705.5
Abstract

Summary: Staphylococcus aureus causes the majority of skin and soft tissue infections, but this pathogen only transiently colonizes healthy skin. However, this transient skin exposure enables S. aureus to transition to infection. The initial adhesion of S. aureus to skin corneocytes is mediated by surface protein G (SasG). Here, phylogenetic analyses reveal the presence of two major divergent SasG alleles in S. aureus: SasG-I and SasG-II. Structural analyses of SasG-II identify a nonaromatic arginine in the binding pocket of the lectin subdomain that mediates adhesion to corneocytes. Atomic force microscopy and corneocyte adhesion assays indicate that SasG-II can bind to a broader variety of ligands than SasG-I. Glycosidase treatment results in different binding profiles between SasG-I and SasG-II on skin cells. In addition, SasG-mediated adhesion is recapitulated using differentiated N/TERT keratinocytes. Our findings indicate that SasG-II has evolved to adhere to multiple ligands, conferring a distinct advantage to S. aureus during skin colonization.

Published
2024
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2. Eubacterium callanderi bacteremia: A case report

Author

Chunyi Zhou, Casey Schwee, Ronald E. Matovu, Jessica D. Wiley, Michael R. Wiley, Bennett J. Berning, Peter C. Iwen, and Paul D. Fey

Subjects
Eubacterium callanderi bacteremia, Anaerobic bacteremia, Infectious and parasitic diseases, RC109-216
Abstract

Eubacterium species are a group of obligated anaerobic gram-positive bacilli that are recognized as commensals of the gastrointestinal tract flora. Cases of bacteremia mediated by Eubacterium are rare. This report describes a case of bacteremia caused by Eubacterium callanderi in an 82-year-old female with a history of a cecal perforation secondary to an obstructing sigmoid stricture. The results showed the utility of using whole genome sequencing to identify the causative agent and underlined the significance to identify anaerobic organisms in diagnostic microbiology practice and to perform antimicrobial susceptibility testing to guide therapy and enhance patient outcomes.

Published
2024
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3. A critical role for staphylococcal nitric oxide synthase in controlling flavohemoglobin toxicity

Author

Ryan M. Singh, Sujata S. Chaudhari, Sasmita Panda, Elizabeth H. Hutfless, Cortney E. Heim, Dhananjay Shinde, Abdulelah A. Alqarzaee, Margaret Sladek, Vineet Kumar, Matthew C. Zimmerman, Paul D. Fey, Tammy Kielian, and Vinai C. Thomas

Subjects
Staphylococcus epidermidis, Bacterial nitric oxide synthase, Flavohemoglobin, Superoxide, Respiration, Medicine (General), R5-920, Biology (General), QH301-705.5
Abstract

Most coagulase-negative staphylococcal species, including the opportunistic pathogen Staphylococcus epidermidis, struggle to maintain redox homeostasis and grow under nitrosative stress. Under these conditions, growth can only resume once nitric oxide (NO) is detoxified by the flavohemoglobin Hmp. Paradoxically, S. epidermidis produces endogenous NO through its genetically encoded nitric oxide synthase (seNOS) and heavily relies on its activity for growth. In this study, we investigate the basis of the growth advantage attributed to seNOS activity. Our findings reveal that seNOS supports growth by countering Hmp toxicity. S. epidermidis relies on Hmp activity for its survival in the host under NO stress. However, in the absence of nitrosative stress, Hmp generates significant amounts of the harmful superoxide radical (O2•-) from its heme prosthetic group which impedes growth. To limit Hmp toxicity, nitrite (NO2−) derived from seNOS promotes CymR-CysK regulatory complex activity, which typically regulates cysteine metabolism, but we now demonstrate to also repress hmp transcription. These findings reveal a critical mechanism through which the bacterial NOS-Hmp axis drives staphylococcal fitness.

Published
2023
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4. Contributors

Author

Kjersti Aagaard, Fredrick M. Abrahamian, Ban Mishu Allos, David R. Andes, Fred Y. Aoki, Michael A. Apicella, Kevin L. Ard, Cesar A. Arias, David M. Aronoff, Michael H. Augenbraun, Francisco Averhoff, Dimitri T. Azar, Larry M. Baddour, Lindsey R. Baden, Carol J. Baker, Ronald C. Ballard, Gerard R. Barber, Scott D. Barnes, Dan H. Barouch, Alan D. Barrett, Miriam Baron Barshak, Sridhar V. Basavaraju, Byron E. Batteiger, Stephen G. Baum, Arnold S. Bayer, J. David Beckham, Susan E. Beekmann, Beth P. Bell, John E. Bennett, Dennis A. Bente, Elie F. Berbari, Jonathan Berman, Joseph S. Bertino, Adarsh Bhimraj, Holly H. Birdsall, Alan L. Bisno, Brian G. Blackburn, Lucas S. Blanton, Martin J. Blaser, Thomas P. Bleck, Nicole M.A. Blijlevens, David A. Bobak, William Bonnez, John C. Boothroyd, Luciana L. Borio, Patrick J. Bosque, John Bower, Robert W. Bradsher, Itzhak Brook, Kevin E. Brown, Patricia D. Brown, Barbara A. Brown-Elliott, Roberta L. Bruhn, Amy E. Bryant, Eileen M. Burd, Jane C. Burns, Larry M. Bush, Stephen B. Calderwood, Luz Elena Cano, Charles C.J. Carpenter, Mary T. Caserta, Elio Castagnola, Richard E. Chaisson, Henry F. Chambers, Stephen J. Chapman, James D. Chappell, Lea Ann Chen, Sharon C-A Chen, Anthony W. Chow, Rebecca A. Clark, Jeffrey I. Cohen, Myron S. Cohen, Ronit Cohen-Poradosu, Susan E. Cohn, Mark Connors, Lawrence Corey, Mackenzie L. Cottrell, Timothy L. Cover, Heather L. Cox, William A. Craig, Kent B. Crossley, Clyde S. Crumpacker, James W. Curran, Bart J. Currie, Erika D'Agata, Inger K. Damon, Rabih O. Darouiche, Roberta L. DeBiasi, George S. Deepe, Carlos del Rio, Andrew S. Delemos, Frank R. DeLeo, Gregory P. DeMuri, Peter Densen, Terence S. Dermody, Robin Dewar, James H. Diaz, Carl W. Dieffenbach, Jules L. Dienstag, Yohei Doi, Raphael Dolin, J. Peter Donnelly, Michael S. Donnenberg, Gerald R. Donowitz, Philip R. Dormitzer, James M. Drake, J. Stephen Dumler, J. Stephen Dummer, Herbert L. DuPont, David T. Durack, Marlene L. Durand, Paul H. Edelstein, Michael B. Edmond, John E. Edwards, Morven S. Edwards, George M. Eliopoulos, Richard T. Ellison, Timothy P. Endy, N. Cary Engleberg, Hakan Erdem, Joel D. Ernst, Peter B. Ernst, Rick M. Fairhurst, Jessica K. Fairley, Stanley Falkow, Ann R. Falsey, Anthony S. Fauci, Thomas Fekete, Paul D. Fey, Steven M. Fine, Daniel W. Fitzgerald, Anthony R. Flores, Derek Forster, Vance G. Fowler, David O. Freedman, Arthur M. Friedlander, John N. Galgiani, John I. Gallin, Robert C. Gallo, Tejal N. Gandhi, Wendy S. Garrett, Charlotte A. Gaydos, Thomas W. Geisbert, Jeffrey A. Gelfand, Steven P. Gelone, Dale N. Gerding, Anne A. Gershon, Janet R. Gilsdorf, Ellie J.C. Goldstein, Fred M. Gordin, Paul S. Graman, M. Lindsay Grayson, Jeffrey Bruce Greene, Patricia M. Griffin, David E. Griffith, Richard L. Guerrant, H. Cem Gul, David A. Haake, David W. Haas, Charles Haines, Caroline Breese Hall, Joelle Hallak, Scott A. Halperin, Margaret R. Hammerschlag, Rashidul Haque, Jason B. Harris, Claudia Hawkins, Roderick J. Hay, Craig W. Hedberg, David K. Henderson, Donald A. Henderson, Kevin P. High, Adrian V.S. Hill, David R. Hill, Alan R. Hinman, Martin S. Hirsch, Aimee Hodowanec, Tobias M. Hohl, Steven M. Holland, Robert S. Holzman, Edward W. Hook, David C. Hooper, Thomas M. Hooton, Harold W. Horowitz, C. Robert Horsburgh, James M. Horton, Duane R. Hospenthal, Kevin Hsueh, James M. Hughes, Noreen A. Hynes, Nicole M. Iovine, Jonathan R. Iredell, Michael G. Ison, J. Michael Janda, Edward N. Janoff, Eric C. Johannsen, Angela D.M. Kashuba, Dennis L. Kasper, Donald Kaye, Keith S. Kaye, Kenneth M. Kaye, James W. Kazura, Jay S. Keystone, Rima Khabbaz, David A. Khan, Yury Khudyakov, Rose Kim, Charles H. King, Louis V. Kirchhoff, Jerome O. Klein, Michael Klompas, Bettina M. Knoll, Kirk U. Knowlton, Jane E. Koehler, Stephan A. Kohlhoff, Eija Könönen, Dimitrios P. Kontoyiannis, Igor J. Koralnik, Poonum S. Korpe, Anita A. Koshy, Joseph A. Kovacs, Phyllis Kozarsky, John Krieger, Andrew T. Kroger, Matthew J. Kuehnert, Nalin M. Kumar, Merin Elizabeth Kuruvilla, Regina C. LaRocque, James E. Leggett, Helena Legido-Quigley, Paul N. Levett, Donald P. Levine, Matthew E. Levison, Alexandra Levitt, Russell E. Lewis, W. Conrad Liles, Aldo A.M. Lima, Ajit P. Limaye, W. Ian Lipkin, Nathan Litman, Bennett Lorber, Ruth Ann Luna, Conan MacDougall, Rob Roy, Philip A. Mackowiak, Lawrence C. Madoff, Alan J. Magill, James H. Maguire, Frank Maldarelli, Lewis Markoff, Jeanne M. Marrazzo, Thomas J. Marrie, Thomas Marth, David H. Martin, Gregory J. Martin, Francisco M. Marty, Melanie Jane Maslow, Henry Masur, Alison Mawle, Kenneth H. Mayer, John T. McBride, James S. McCarthy, William M. McCormack, Catherine C. McGowan, Kenneth McIntosh, Paul S. Mead, Malgorzata Mikulska, Robert F. Miller, Samuel I. Miller, David H. Mitchell, John F. Modlin, Rajal K. Mody, Robert C. Moellering, Matthew Moffa, Susan Moir, José G. Montoya, Thomas A. Moore, Philippe Moreillon, J. Glenn Morris, Caryn Gee Morse, Robin Moseley, Robert S. Munford, Edward L. Murphy, Timothy F. Murphy, Barbara E. Murray, Clinton K. Murray, Patrick R. Murray, Daniel M. Musher, Jerod L. Nagel, Esteban C. Nannini, Anna Narezkina, Theodore E. Nash, William M. Nauseef, Jennifer L. Nayak, Marguerite A. Neill, Judith A. O'Donnell, Christopher A. Ohl, Pablo C. Okhuysen, Andrew B. Onderdonk, Steven M. Opal, Walter A. Orenstein, Douglas R. Osmon, Michael T. Osterholm, Stephen M. Ostroff, Michael N. Oxman, Slobodan Paessler, Andrea V. Page, Manjunath P. Pai, Tara N. Palmore, Raj Palraj, Peter G. Pappas, Mark S. Pasternack, Thomas F. Patterson, Deborah Pavan-Langston, David A. Pegues, Robert L. Penn, John R. Perfect, Stanley Perlman, Brett W. Petersen, Phillip K. Peterson, William A. Petri, Cathy A. Petti, Jennifer A. Philips, Julie V. Philley, Michael Phillips, Larry K. Pickering, Peter Piot, Jason M. Pogue, Aurora Pop-Vicas, Cynthia Portal-Celhay, John H. Powers, Richard N. Price, Yok-Ai Que, Justin D. Radolf, Sanjay Ram, Didier Raoult, Jonathan I. Ravdin, Stuart C. Ray, Annette C. Reboli, Marvin S. Reitz, David A. Relman, Cybèle A. Renault, Angela Restrepo, John H. Rex, Elizabeth G. Rhee, Norbert J. Roberts, José R. Romero, Alan L. Rothman, Craig R. Roy, Kathryn L. Ruoff, Mark E. Rupp, Charles E. Rupprecht, Thomas A. Russo, William A. Rutala, Edward T. Ryan, Amar Safdar, Mohammad M. Sajadi, Juan C. Salazar, Juan Carlos Sarria, Maria C. Savoia, Paul E. Sax, W. Michael Scheld, Joshua T. Schiffer, David Schlossberg, Thomas Schneider, Anne Schuchat, Jane R. Schwebke, Cynthia L. Sears, Leopoldo N. Segal, Parham Sendi, Kent A. Sepkowitz, Edward J. Septimus, Alexey Seregin, Stanford T. Shulman, George K. Siberry, Omar K. Siddiqi, Costi D. Sifri, Michael S. Simberkoff, Francesco R. Simonetti, Kamaljit Singh, Nina Singh, Upinder Singh, Scott W. Sinner, Sumathi Sivapalasingam, Leonard N. Slater, A. George Smulian, Jack D. Sobel, M. Rizwan Sohail, David E. Soper, Tania C. Sorrell, James M. Steckelberg, Allen C. Steere, Neal H. Steigbigel, James P. Steinberg, David S. Stephens, Timothy R. Sterling, David A. Stevens, Dennis L. Stevens, Jacob Strahilevitz, Charles W. Stratton, Anthony F. Suffredini, Kathryn N. Suh, Mark S. Sulkowski, Morton N. Swartz, Thomas R. Talbot, C. Sabrina Tan, Ming Tan, Chloe Lynne Thio, David L. Thomas, Lora D. Thomas, Stephen J. Thomas, Anna R. Thorner, Angela María Tobón, Edmund C. Tramont, John J. Treanor, Jason Trubiano, Athe M.N. Tsibris, Allan R. Tunkel, Ronald B. Turner, Kenneth L. Tyler, Ahmet Uluer, Diederik van de Beek, Walter J.F.M. van der Velden, Edouard G. Vannier, Trevor C. Van, James Versalovic, Claudio Viscoli, Ellen R. Wald, Matthew K. Waldor, David H. Walker, Richard J. Wallace, Edward E. Walsh, Stephen R. Walsh, Peter D. Walzer, Christine A. Wanke, Cirle A. Warren, Ronald G. Washburn, Valerie Waters, David J. Weber, Michael D. Weiden, Geoffrey A. Weinberg, Daniel J. Weisdorf, Louis M. Weiss, David F. Welch, Thomas E. Wellems, Richard P. Wenzel, Melinda Wharton, A. Clinton White, Richard J. Whitley, Walter R. Wilson, Glenn W. Wortmann, William F. Wright, Jo-Anne H. Young, Vincent B. Young, Nadezhda Yun, Werner Zimmerli, Stephen H. Zinner, and John J. Zurlo

Published
2015

5. Staphylococcus epidermidis and Other Coagulase-Negative Staphylococci

Author

Paul D. Fey and Mark E. Rupp

Subjects
Staphylococcus saprophyticus, biology, business.industry, Biofilm, Staphylococcus lugdunensis, biology.organism_classification, medicine.disease, Microbiology, Staphylococcus epidermidis, Bacteremia, Staphylococcus haemolyticus, Medicine, Coagulase, business
Published
2015

6. [19] In Vivo Models to evaluate adhesion and biofilm formation by staphylococcus epidermidis

Author

Paul D. Fey and Mark E. Rupp

Subjects
Bacterial adhesin, In vivo, Staphylococcus epidermidis, Biofilm, medicine, Biomaterial, Adhesion, Biology, medicine.disease_cause, biology.organism_classification, Staphylococcus, In vitro, Microbiology
Abstract

Publisher Summary The chapter discusses In Vivo models to evaluate adhesion and biofilm formation by Staphylococcus epidermidis. Adhesion of Staphylococcus epiderrnidis to biomaterials is a pivotal event in the pathogenesis of prosthetic device infections. Adhesion is a complex multistep event that is governed by various adhesions at different stages of the process. The early stages of adhesion are characterized by initial attachment of organisms to the surface of the biomaterial, whereas later stages of adhesion involve cellular aggregation and elaboration of biofilm. The pathogenesis of biomaterial-based infections involves interactions amongst the host, the microbe, and the biomaterial that are impossible to duplicate in in vitro systems. Therefore, to study the adhesion of S. epidermidis to biomaterials and the pathogenesis of prosthetic device infections, investigators have developed in vivo models to mimic the human condition. This chapter describes two in vivo models used in laboratory to study the pathogenic significance of adhesins and biofilm production of S. epidermidis.

Published
2001

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