1. Generation of a healthy heavy smoker patient-derived induced pluripotent stem cell line UHOMi007-A from peripheral blood mononuclear cells.
- Author
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Ahmed E, Fieldès M, Bourguignon C, Mianné J, Petit A, Amel N, Foisset F, Bourdais C, Vachier I, Assou S, De Vos J, and Bourdin A
- Subjects
- Humans, Male, Cell Line, Cell Differentiation, Smokers, Cellular Reprogramming, Induced Pluripotent Stem Cells metabolism, Induced Pluripotent Stem Cells cytology, Leukocytes, Mononuclear metabolism, Leukocytes, Mononuclear cytology
- Abstract
Human pluripotent stem cells (hiPSC) represent a unique opportunity to model lung development and chronic bronchial diseases. We generated a hiPSC line from a highly characterized healthy heavy smoker male donor free from emphysema or tobacco related disease. Peripheral blood mononuclear cells (PBMCs) were reprogrammed using integration-free Sendai virus. The cell line had normal karyotype, expressed pluripotency hallmarks, and differentiated into the three primary germ layers. The reported UHOMi007-A iPSC line may be used as a control to model lung development, study human chronic bronchial diseases and drug testing., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 University Hospital Centre of Montpellier. Published by Elsevier B.V. All rights reserved.)
- Published
- 2024
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