1. First-generation monoclonal antibodies identifying the human leukotriene B(4) receptor-1.
- Author
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Pettersson A, Boketoft A, Sabirsh A, Nilsson NE, Kotarsky K, Olde B, and Owman C
- Subjects
- 3T3 Cells, Animals, Antibody Specificity, Flow Cytometry, Genes, Reporter, HeLa Cells, Humans, Immunohistochemistry, Mice, Receptors, Leukotriene B4 genetics, Recombinant Proteins analysis, Recombinant Proteins immunology, Transfection, Antibodies, Monoclonal, Receptors, Leukotriene B4 analysis, Receptors, Leukotriene B4 immunology
- Abstract
The leukotriene B(4) receptor (BLTR) is a seven-transmembrane chemoattractant receptor that is important in pro-inflammatory responses. We have produced the first widely applicable monoclonal antibodies against the human BLTR and confirmed the antibody specificity using flow cytometric analysis of three different cell lines stably expressing the recombinant receptor. The antibodies did not cross-react with the recently cloned second LTB(4) receptor, BLTR2, or the Cys LT1 and Cys LT2 receptors. Functional analysis in combination with two-color flow cytometry showed that the BLTR antibodies bind to cells that are activated by LTB(4). The antibodies were shown to recognize BLTR in cell ELISA and immunocytochemistry. Endogenous expression of BLTR in CD15-positive blood leukocytes and in differentiated HL-60 cells was also demonstrated with the antibodies., (Copyright 2000 Academic Press.)
- Published
- 2000
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