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1. Modified CRISPR/Cas9 mediated generation of two MKK7 knockout human embryonic stem cell lines

Author

C.H. Cole Sims, Matias I. Autio, Adriana Buskin, Christine Cheung, Rakesh Heer, Roger S.Y. Foo, and Xin Wang

Subjects
Biology (General), QH301-705.5
Abstract

Two cell lines were generated by CRISPR/Cas9 mediated knockout of MKK7 (MAP2K7) by removal of exon 1 or exons 4 through 7. These knockouts were confirmed at the transcript and protein levels. These hESCs are pluripotent and maintain tri-lineage differentiation capacity. These cell lines are a useful resource for studying MKK7 function in humans.

Published
2021
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