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Your search keyword '"Satish N. Nadig"' showing total 9 results
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9 results on '"Satish N. Nadig"'

1. Direct NP- A cost-effective extraction-free RT-qPCR based test for SARS-CoV-2

Author

Rasesh Y. Parikh, Satish N. Nadig, Shikhar Mehrotra, Philip H. Howe, and Vamsi K. Gangaraju

Subjects
COVID-19, SARS-CoV-2, Dualplex, RT-qPCR, Broad-scale, Test, Science (General), Q1-390, Social sciences (General), H1-99
Abstract

Over 2.4 million daily total tests are currently being performed for SARS-CoV-2, in the United States. The most common SARS-CoV-2 tests require RNA extraction and purification. Extraction of RNA is a time-consuming and costly step that requires a constant supply of reagents and accessories. With the current testing demand, the supply chain remains the bottleneck for RNA extraction. Here, we report Direct NP- a cost-effective extraction-free RT-qPCR based dualplex test for SARS-CoV-2 from Nasopharyngeal (NP) swab specimens. Direct NP detects SARS-CoV-2 viral RNA from heat-denatured patient specimens using a dualplex RT-qPCR assay. Direct NP showed 92.5% positive percentage agreement (PPA) (95% Confidence Interval (CI) = 79.61%–98.43%) and 97% negative percent agreement (NPA) (95% CI = 89.11–100%) with the CDC assay. Direct NP reduces the cost per test to $2, making it suitable for broad-scale testing while lowering the cost burden on the healthcare system.

Published
2022
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2. Comparative analysis of antibodies to SARS-CoV-2 between asymptomatic and convalescent patients

Author

Connor J. Dwyer, Colleen A. Cloud, Cindy Wang, Philip Heidt, Paramita Chakraborty, Tara F. Duke, Shannon McGue, Braxton Jeffcoat, Jaclyn Dunne, Logan Johnson, Seungho Choi, Georges J. Nahhas, Amy S. Gandy, Nikolina Babic, Frederick S. Nolte, Philip Howe, Besim Ogretmen, Vamsi K. Gangaraju, Stephen Tomlinson, Brian Madden, Tracy Bridges, Patrick A. Flume, John Wrangle, Mark P. Rubinstein, Prabhakar K. Baliga, Satish N. Nadig, and Shikhar Mehrotra

Subjects
Infection control in health technology, Immunology, Virology, Science
Abstract

Summary: The SARS-CoV-2 viral pandemic has induced a global health crisis, which requires more in-depth investigation into immunological responses to develop effective treatments and vaccines. To understand protective immunity against COVID-19, we screened over 60,000 asymptomatic individuals in the Southeastern United States for IgG antibody positivity against the viral Spike protein, and approximately 3% were positive. Of these 3%, individuals with the highest anti-S or anti-RBD IgG level showed a strong correlation with inhibition of ACE2 binding and cross-reactivity against non-SARS-CoV-2 coronavirus S-proteins. We also analyzed samples from 94 SARS-CoV-2 patients and compared them with those of asymptomatic individuals. SARS-CoV-2 symptomatic patients had decreased antibody responses, ACE2 binding inhibition, and antibody cross-reactivity. Our study shows that healthy individuals can mount robust immune responses against SARS-CoV-2 without symptoms. Furthermore, IgG antibody responses against S and RBD may correlate with high inhibition of ACE2 binding in individuals tested for SARS-CoV-2 infection or post vaccination.

Published
2021
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3. Biliary restoration using a combined endoscopic-percutaneous approach following 'orphan duct syndrome' after pediatric liver transplantation

Author

Patrick B. Dennis, Elizabeth K. Nadeau, Jared White, Ricardo Yamada, D. Thor Johnson, B. Joseph Elmunzer, Vinayak Rohan, Nagraj Kasi, and Satish N. Nadig

Subjects
Liver transplantation, Biliary restoration, Orphan duct, Pediatrics, RJ1-570, Surgery, RD1-811
Abstract

A 2-year-old male with arginosuccinate lyase deficiency underwent left lateral segment liver transplantation complicated by “orphan duct syndrome (Celik et al., 2019) [1]” and biliary leak. After revision of the Roux-en-Y anastomosis, biliary drainage was still impaired. The excluded bile duct was diagnosed and a biliary restoration (or neo-duct) using a combined endoscopic and percutaneous approach was created by Gastroenterology and Interventional Radiology. To our knowledge, this case report represents the first combined endoscopic-percutaneous biliary restoration procedure performed in a pediatric patient.

Published
2020
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4. 'Pushing the margin:' utilization of renal autotransplantation to achieve complete resection in vena caval leiomyosarcomas

Author

Joseph Anderson, Vinayak Rohan, Helen Grey, and Satish N. Nadig

Subjects
Pediatrics, RJ1-570, Surgery, RD1-811
Abstract

We report the case of a 19-year-old female who underwent resection of the sub- and infrahepatic inferior vena cava (IVC) with concomitant left kidney autotransplantation for leiomyosarcoma. Based on the available literature, this is the youngest patient reported undergoing IVC resection and reconstruction with concomitant renal autotransplant. We review the literature, presentation, and a unique treatment option for this infrequent pathology. Keywords: Leiomyosarcoma, Renal autotransplant, Leiomyosarcoma of the inferior vena cava, Leiomyosarcoma with renal vein involvement, Pediatric kidney autotransplant

Published
2020
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5. Biliary restoration using a combined endoscopic-percutaneous approach following 'orphan duct syndrome' after pediatric liver transplantation

Author

Elizabeth K. Nadeau, D. Thor Johnson, Vinayak Rohan, Ricardo Yamada, B. Joseph Elmunzer, Nagraj Kasi, Jared White, Patrick B Dennis, and Satish N. Nadig

Subjects
medicine.medical_specialty, medicine.medical_treatment, lcsh:Surgery, Liver transplantation, Anastomosis, 03 medical and health sciences, 0302 clinical medicine, medicine, Arginosuccinate lyase deficiency, Orphan duct, Biliary drainage, medicine.diagnostic_test, business.industry, Bile duct, lcsh:RJ1-570, Interventional radiology, lcsh:Pediatrics, lcsh:RD1-811, Percutaneous approach, Surgery, medicine.anatomical_structure, Biliary restoration, 030220 oncology & carcinogenesis, Pediatrics, Perinatology and Child Health, 030211 gastroenterology & hepatology, business, Duct (anatomy)
Abstract

A 2-year-old male with arginosuccinate lyase deficiency underwent left lateral segment liver transplantation complicated by “orphan duct syndrome (Celik et al., 2019) [1]” and biliary leak. After revision of the Roux-en-Y anastomosis, biliary drainage was still impaired. The excluded bile duct was diagnosed and a biliary restoration (or neo-duct) using a combined endoscopic and percutaneous approach was created by Gastroenterology and Interventional Radiology. To our knowledge, this case report represents the first combined endoscopic-percutaneous biliary restoration procedure performed in a pediatric patient.

Published
2020

6. Approaches to the Induction of Tolerance

Author

Satish N. Nadig, G Warnecke, and Kathryn J. Wood

Subjects
medicine.medical_specialty, business.industry, medicine.medical_treatment, Transplant recipient, Immunosuppression, Disease, Biology, Organ transplantation, Immunosuppressive drug, Transplantation medicine, Immunity, medicine, Intensive care medicine, business
Abstract

Organ transplants are currently protected from rejection by treating recipients with potent immunosuppressive agents that suppress general immunity. While general immunosuppression does effectively protect allografts from acute rejection in the short term, chronic rejection presents a problem in the long term, and side effects from life-long non-specific immunosuppression can result in organ toxicity, infections, posttransplant malignancy and cardiovascular disease. To avoid the problem of needing these immunosuppressive drugs, early research from the forefathers of transplantation medicine showed us that achieving immunological tolerance is possible and clearly a worthy goal. However, fully understanding the mechanisms of tolerance and practically implementing protocols to reliably and safely induce tolerance have remained elusive. This book chapter delves into the history of tolerance research in organ transplantation and brings us to the current era of what we know about mechanisms that control tolerance. Finally, we will discuss how researchers are using this knowledge to design new protocols to induce tolerance in an attempt to reduce transplant recipient dependency on general immunosuppressive drug therapy.

Published
2016

8. Contributors

Author

Ghassan K. Abou-Alfa, N. Volkan Adsay, Timothy Akhurst, Farzad Alemi, David Allegra, Peter J. Allen, Christopher D. Anderson, Taku Aoki, Ramakrishnan Arulraj, Rebecca Ann C. Auer, Béatrice Aussilhou, Joseph Awad, Chad G. Ball, Zubin M. Bamboat, Claudio Bassi, Christoph D. Becker, Pierre Bedossa, Jacques Belghiti, Paul Belletrutti, Anton J. Bilchik, Leslie H. Blumgart, Philippus C. Bornman, Cherif Boutros, Jonathan R. Brody, Lynn A. Brody, Antoine Brouquet, Karen T. Brown, William R. Brugge, Jordi Bruix, David A. Bruno, Elizabeth M. Brunt, Markus W. Büchler, Justin M. Burns, Giovanni Butturini, Mark P. Callery, David L. Carr-Locke, Charles Cha, See Ching Chan, William C. Chapman, Daniel Cherqui, Clifford S. Cho, Jin Wook Chung, Bryan Clary, Andrei Cocieru, Carlos M. Contreras, Kendra D. Conzen, Carlos U. Corvera, Anne M. Covey, Jeffrey S. Crippin, Hany Dabbous, Michael I. D’Angelica, Michael Darcy, Mark Davenport, Jeroen De Jonge, Ronald P. DeMatteo, Achilles A. Demetriou, Niraj M. Desai, Eduardo De Santibañes, Terry S. Desser, Kiran K. Dhanireddy, Safi Dokmak, M.B. Majella Doyle, Truman M. Earl, Tomoki Ebata, Yousef El-Gohary, Andrew S. Epstein, N. Joseph Espat, Sheung Tat Fan, Olivier Farges, Carlos Fernandez-Del Castillo, Cristina R. Ferrone, Mary Fischer, James E. Fisher, Yuman Fong, Alejandro Forner, Scott L. Friedman, Stefan Fritz, John R. Galloway, Hans Gerdes, George Getrajdman, Paula Ghaneh, Giorgia Ghittoni, George Gittes, Karyn A. Goodman, Gregory J. Gores, Eduardo Gotuzzo, Dirk J. Gouma, Paul D. Greig, Christopher M. Halloran, Neil A. Halpern, Lucy E. Hann, Ewen M. Harrison, Werner Hartwig, Julie K. Heimbach, William S. Helton, Alan W. Hemming, J. Michael Henderson, Amelia J. Hessheimer, Asher Hirshberg, Alice Ho, Michael G. House, Chung-Bao Hsieh, Kuo-Feng Hsu, David A. Iannitti, Hiroshi Imamura, Clem W. Imrie, William R. Jarnagin, Roger L. Jenkins, Stefan Kahl, Ivan Kangrga, Kaitlyn Kelly, Nancy E. Kemeny, Eugene P. Kennedy, Robert K. Kerlan Jr, Adeel S. Khan, Saboor Khan, T. Peter Kingham, Allan D. Kirk, Yuichi Kitagawa, David S. Klimstra, Michael D. Kluger, Stuart J. Knechtle, Peter J. Kneuertz, Jonathan Koea, Alan J. Koffron, Norihiro Kokudo, David A. Kooby, Kevin Korenblat, Robert M. Krasny, Jake Krige, Robert C. Kurtz, Matthew P. Landman, Michael P. La Quaglia, Nicholas F. LaRusso, Kwan N. Lau, Konstantinos N. Lazaridis, Markus M. Lerch, Joseph B. Lillegard, Keith D. Lillemoe, David C. Linehan, Susan Logan, Benjamin Loveday, Jeffrey A. Lowell, David C. Madoff, Shishir K. Maithel, Ali W. Majeed, Masatoshi Makuuchi, Peter Malfertheiner, Mark Mamlouk, Giovanni Marchegiani, Luis A. Marcos, James F. Markmann, J. Wallis Marsh, Maureen Martin, Robert C.G. Martin II, Dipen M. Maru, Francisco Juan Mattera, Julia V. Mayerle, Oscar M. Mazza, Ian D. McGilvray, Colin J. McKay, Jose A. Melendez, James J. Mezhir, George Miller, Klaus Mönkemüller, Tricia A. Moo-Young, Satish N. Nadig, Masato Nagino, Alexander Nagle, David M. Nagorney, Atilla Nakeeb, Hector E. Nazario, Geir Nedredal, John P. Neoptolemos, James Neuberger, Douglas L. Nguyen, Yuji Nimura, Scott L. Nyberg, John G. O’Grady, Risteard O’laoide, Kim Marie Olthoff, Mark S. Orloff, Marshall J. Orloff, Susan L. Orloff, Rish K. Pai, Katia Papalezova, Valérie Paradis, Rowan W. Parks, Steven D. Passik, Stephen M. Pastores, Patrick Pessaux, Maxim S. Petrov, Venu G. Pillarisetty, James Pingpank Jr, C. Wright Pinson, Sofija Pitka, Henry A. Pitt, James J. Pomposelli, Wande B. Pratt, Richard A. Prinz, Srinivas R. Puli, Florencia G. Que, Valentina Ravetta, Maria E. Reig, Ahsun Riaz, Barrie S. Rich, David W. Rittenhouse, John Roberts, Piera Robson, Flavio G. Rocha, Seaborn A. Roddenbery V, Carlos Rodriguez de Lopes, Garrett R. Roll, Alex S. Rosemurgy II, Charles B. Rosen, Sandro Rossi, Pierre F. Saldinger, Riad Salem, Leonard B. Saltz, Norberto J. Sanchez, Charbel Sandroussi, Tsuyoshi Sano, O. Scatton, Mark Schattner, William P. Schecter, Suzanne C. Schiffman, C. Max Schmidt, Helmut Schoellnast, Lawrence H. Schwartz, Ross W. Shepherd, Masafumi Shimoda, Hosein Shokouh-Amiri, Nasir H. Siddiqi, Stephen B. Solomon, Nathaniel J. Soper, Thomas E. Starzl, Michael Steer, Lygia Stewart, Oliver Strobel, Jerrold Teitcher, Angélica Terashima, Sylvain Terraz, William E.G. Thomas, Guido Torzilli, James F. Trotter, Yumirle P. Turmelle, Christine Van Cott, Andrea Vannucci, Eric vanSonnenberg, Jean-Nicolas Vauthey, Diana Vetter, Valerie Vilgrain, Alejandra Villamil, Louis Voigt, Charles M. Vollmer Jr, Jack R. Wands, Julia Wattacheril, Sharon Weber, Matthew Weiss, Jürgen Weitz, Jens Werner, Antony M. Wheatley, John A. Windsor, Corinne Winston, Jordan Winter, Agnieszka K. Witkiewicz, John Wong, Charles J. Yeo, Theresa P. Yeo, Chang Jin Yoon, Adam Yopp, Gazi Zibari, and Randall Zuckerman

Published
2012
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9. Contributors

Author

Richard D.M. Allen, John M. Barry, Amit Basu, Bryan N. Becker, A.D. Billiau, Cristina Bordea, J. Andrew Bradley, Nicholas R. Brook, Michael Campbell, Jeremy R. Chapman, Robert B. Colvin, A. Benedict Cosimi, David Cranston, Abdallah S. Daar, Margaret J. Dallman, John A. Davis, Francis L. Delmonico, Oliver J. Dyar, Robert B. Ettenger, Jay A. Fishman, Andria L. Ford, Julie Franc-Guimond, Patricia M. Franklin, Susan V. Fuggle, James M. Gloor, Ricardo González, Angelika C. Gruessner, Rainer W.G. Gruessner, David Hamilton, Ajay K. Israni, Barry D. Kahan, Bertram L. Kasiske, Allan D. Kirk, Stuart J. Knechtle, Simon R. Knight, Dicken S.C. Ko, Takashi Kobayashi, Aoife Lally, Jin-Moo Lee, Henri G.D. Leuvenink, Michael R. Lucey, C. Mathieu, Shamila Mauiyyedi, Paula J. Mohacsi, Robert A. Montgomery, M. Rafique Moosa, Peter J. Morris, Satish N. Nadig, Brian J. Nankivell, Lisa Nanovic, Michael L. Nicholson, J. Pirenne, John D. Pirsch, Rutger J. Ploeg, Pornpimol Rianthavorn, Neil K.I. Russell, Nasia Safdar, Adnan Said, Minnie M. Sarwal, John W. Sear, Ron Shapiro, Daniel Shoskes, Christopher E. Simpkins, B. Sprangers, Mark D. Stegall, David E.R. Sutherland, Craig J. Taylor, John F. Thompson, E. van Etten, Vanessa Venning, Katie D. Vo, Mark Waer, Gregor Warnecke, Christopher J.E. Watson, Jennifer T. Wells, Fenella Wojnarowska, Kathryn J. Wood, Kenneth E. Wood, and Linda Wright

Published
2008
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