1. Direct anti-inflammatory effects of granulocyte colony-stimulating factor (G-CSF) on activation and functional properties of human T cell subpopulations in vitro.
- Author
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Malashchenko VV, Meniailo ME, Shmarov VA, Gazatova ND, Melashchenko OB, Goncharov AG, Seledtsova GV, and Seledtsov VI
- Subjects
- Adult, Anti-Inflammatory Agents metabolism, Anti-Inflammatory Agents pharmacology, CD28 Antigens immunology, CD4-Positive T-Lymphocytes immunology, Cytokines biosynthesis, Female, Granulocyte Colony-Stimulating Factor pharmacology, Healthy Volunteers, Humans, Immunologic Memory drug effects, Interferon-gamma immunology, Interleukin-2 Receptor alpha Subunit immunology, Interleukin-2 Receptor alpha Subunit metabolism, Macrophage Activation drug effects, Male, T-Lymphocyte Subsets immunology, Granulocyte Colony-Stimulating Factor metabolism, Lymphocyte Activation drug effects, T-Lymphocytes drug effects
- Abstract
We investigated the direct effects of human granulocyte colony-stimulating factor (G-CSF) on functionality of human T-cell subsets. CD3
+ T-lymphocytes were isolated from blood of healthy donors by positive magnetic separation. T cell activation with particles conjugated with antibodies (Abs) to human CD3, CD28 and CD2 molecules increased the proportion of cells expressing G-CSF receptor (G-CSFR, CD114) in all T cell subpopulations studied (CD45RA+ /CD197+ naive T cells, CD45RA- /CD197+ central memory T cells, CD45RA- /CD197- effector memory T cells and CD45RA+ /CD197- terminally differentiated effector T cells). Upon T-cell activation in vitro, G-CSF (10.0 ng/ml) significantly and specifically enhanced the proportion of CD114+ T cells in central memory CD4+ T cell compartment. A dilution series of G-CSF (range, 0.1-10.0 ng/ml) was tested, with no effect on the expression of CD25 (interleukin-2 receptor α-chain) on activated T cells. Meanwhile, G-CSF treatment enhanced the proportion of CD38+ T cells in CD4+ naïve T cell, effector memory T cell and terminally differentiated effector T cell subsets, as well as in CD4- central memory T cells and terminally differentiated effector T cells. G-CSF did not affect IL-2 production by T cells; relatively low concentrations of G-CSF down-regulated INF-γ production, while high concentrations of this cytokine up-regulated IL-4 production in activated T cells. The data obtained suggests that G-CSF could play a significant role both in preventing the development of excessive and potentially damaging inflammatory reactivity, and in constraining the expansion of potentially cytodestructive T cells., (Copyright © 2018 Elsevier Inc. All rights reserved.)- Published
- 2018
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