1. In-vitro and in-vivo investigation of wound healing efficacy of Syzygium cumini leaf extracts loaded carboxymethylcellulose film.
- Author
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Ayyanar CB, Bal T, Fahaduddin, Sharma S, Gayathri B, Rinusuba V, Nalini HE, Deepa S, Dharshinii MD, Kharra P, and Sinha A
- Subjects
- Animals, Mice, Cell Line, Escherichia coli drug effects, Rats, Drug Liberation, Staphylococcus aureus drug effects, Male, Wound Healing drug effects, Syzygium chemistry, Carboxymethylcellulose Sodium chemistry, Carboxymethylcellulose Sodium pharmacology, Plant Leaves chemistry, Plant Extracts pharmacology, Plant Extracts chemistry, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents chemistry, Antioxidants pharmacology, Antioxidants chemistry
- Abstract
The study focused on Syzygium cumini Leaf Extract (SCLE) loaded into Carboxymethylcellulose (CMC) film via Solution casting. Phytochemical screening revealed carbohydrates, and HPLC analysis identified quercetin, known for promoting wound healing. FT-IR spectroscopy confirmed various functional groups. X-Ray diffraction (XRD) determined the crystallite size to be 14.58 nm. Field Emission Scanning Electron Microscopy (FESEM) showed the dispersion of extracts, and Energy Dispersive X-ray (EDX) analysis detailed the weight percentages of components. Antibacterial activity tests revealed zones of inhibition for S. aureus (15 mm) and E. coli (11 mm). The film exhibited 63.11 % antioxidant activity at 517 nm with DPPH at a 750 μl sample concentration. Drug release kinetics were also studied. In-vitro wound healing using the L929 cell line showed 83 % healing at a 100 μl concentration. Over 14 days, the treatment group's wounds healed completely within 7 days, unlike the control groups which showed no recovery after 14 days. These findings indicate that the SCLE-CMC film is highly effective in promoting wound healing., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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