1. Coenzyme Q(10) production by immobilized Sphingomonas sp. ZUTE03 via a conversion-extraction coupled process in a three-phase fluidized bed reactor.
- Author
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Qiu L, Ding H, Wang W, Kong Z, Li X, Shi Y, and Zhong W
- Subjects
- Culture Media, Parabens metabolism, Sphingomonas cytology, Terpenes metabolism, Ubiquinone biosynthesis, Bioreactors, Biotechnology methods, Cells, Immobilized metabolism, Sphingomonas metabolism, Ubiquinone analogs & derivatives
- Abstract
A three-phase fluidized bed reactor (TPFBR) was designed to evaluate the potential of CoQ(10) production by gel-entrapped Sphingomonas sp. ZUTE03 via a conversion-extract coupled process. In the reactor, the CoQ(10) yield reached 46.99 mg/L after 8 h of conversion; a high-level yield of about 45 mg/L was maintained even after 15 repetitions (8 h/batch). To fully utilize the residual precursor (para-hydroxybenzoic acid, PHB) in the aqueous phase, the organic phase was replaced with new solution containing 70 mg/L solanesol for each 8 h batch. The CoQ(10) yield of each batch was maintained at a level of about 43 mg/L until the PHB ran out. When solid solanesol was fed to the organic phase for every 8 h batch, CoQ(10) could accumulate and reach a yield of 171.52 mg/L. When solid solanesol and PHB were fed to the conversion system after every 8 h batch, the CoQ(10) yield reached 441.65 mg/L in the organic phase after 20 repetitions, suggesting that the conversion-extract coupled process could enhance CoQ(10) production in the TPFBR., (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Published
- 2012
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