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Thanks to its excellent climate and varied agroecological zones, Nepal has been a significant player in the global rose market. The geographical variety of the nation makes it possible to grow roses all year long, making it a desirable location for rose export and cultivation. The production and export of roses have significantly increased in recent years. The rose trade has increased in Nepal for several reasons. Investment in the industry has been stimulated by government measures to support floriculture and offer incentives to rose farmers. Additionally, the effective distribution of roses to domestic and foreign markets has been made possible by better transportation infrastructure. The growth of the global market has greatly aided the development of Nepal's rose industry. The growth of rose cultivation in Nepal has significantly impacted the economy and employment. It is a good source of revenue through internal sales, export, and other activities that provide value addition. The sector creates direct farm jobs while local communities benefit from indirect employment in supporting industries. In addition, there is increased job opportunities due to seasonal spikes in demand. Rose exports have expanded due to a rise in demand for Nepali roses, particularly in European markets and nearby nations like India. The favorable trade agreements Nepal has with several nations have helped to increase its exports even more. But issues still exist in the rose trade sector. Since maintaining consistent quality is necessary to compete in the international market, quality control and standardization continue to be areas of concern. The rose business in Nepal is also vulnerable to changes in global demand and competition from other nations that grow roses. Further expansion of the industry will help it overcome problems like limited technology and training hence leading to its fast development; this calls for government support as well as market expansion. In conclusion, the rose trade environment in Nepal is a fluid and changing one. Due to favorable circumstances, government assistance, and global demand, the sector has seen significant expansion. Continuous efforts in quality control, market diversification, and innovation are necessary to maintain and grow this sector. The rose trade in Nepal has the potential to significantly boost the nation's agricultural exports while promoting economic growth in rural areas.

Single ventricle heart defects (SVDs) are congenital disorders that result in a variety of complications, including increased ventricular mechanical strain and mixing of oxygenated and deoxygenated blood, leading to heart failure without surgical intervention. Corrective surgery for SVDs are traditionally handled by the Fontan procedure, requiring a vascular conduit for completion. Although effective, current conduits are limited by their inability to aid in pumping blood into the pulmonary circulation. In this report, we propose an innovative and versatile design strategy for a tissue engineered pulsatile conduit (TEPC) to aid circulation through the pulmonary system by producing contractile force. Several design strategies were tested for production of a functional TEPC. Ultimately, we found that porcine extracellular matrix (ECM)-based engineered heart tissue (EHT) composed of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) and primary cardiac fibroblasts (HCF) wrapped around decellularized human umbilical artery (HUA) made an efficacious basal TEPC. Importantly, the TEPCs showed effective electrical and mechanical function. Initial pressure readings from our TEPC in vitro (0.68 mmHg) displayed efficient electrical conductivity enabling them to follow electrical pacing up to a 2 Hz frequency. This work represents a proof of principle study for our current TEPC design strategy. Refinement and optimization of this promising TEPC design will lay the groundwork for testing the construct's therapeutic potential in the future. Together this work represents a progressive step toward developing an improved treatment for SVD patients. STATEMENT OF SIGNIFICANCE: Single Ventricle Cardiac defects (SVD) are a form of congenital disorder with a morbid prognosis without surgical intervention. These patients are treated through the Fontan procedure which requires vascular conduits to complete. Fontan conduits have been traditionally made from stable or biodegradable materials with no pumping activity. Here, we propose a tissue engineered pulsatile conduit (TEPC) for use in Fontan circulation to alleviate excess strain in SVD patients. In contrast to previous strategies for making a pulsatile Fontan conduit, we employ a modular design strategy that allows for the optimization of each component individually to make a standalone tissue. This work sets the foundation for an in vitro, trainable human induced pluripotent stem cell based TEPC., (Copyright © 2019. Published by Elsevier Ltd.)