Search

Your search keyword '"Taccola G"' showing total 3 results
Start Over Author "Taccola G" Publisher elsevier
3 results on '"Taccola G"'

1. Using EMG to deliver lumbar dynamic electrical stimulation to facilitate cortico-spinal excitability.

Author

Taccola G, Gad P, Culaclii S, Ichiyama RM, Liu W, Edgerton VR, Taccola G, Gad P, Culaclii S, Ichiyama RM, Liu W, and Edgerton VR

Abstract

BACKGROUND:Potentiation of synaptic activity in spinal networks is reflected in the magnitude of modulation of motor responses evoked by spinal and cortical input. After spinal cord injury, motor evoked responses can be facilitated by pairing cortical and peripheral nerve stimuli. OBJECTIVE:To facilitate synaptic potentiation of cortico-spinal input with epidural electrical stimulation, we designed a novel neuromodulation method called dynamic stimulation (DS), using patterns derived from hind limb EMG signal during stepping. METHODS:DS was applied dorsally to the lumbar enlargement through a high-density epidural array composed of independent platinum-based micro-electrodes. RESULTS:In fully anesthetized intact adult rats, at the interface array/spinal cord, the temporal and spatial features of DS neuromodulation affected the entire lumbosacral network, particularly the most rostral and caudal segments covered by the array. DS induced a transient (at least 1 min) increase in spinal cord excitability and, compared to tonic stimulation, generated a more robust potentiation of the motor output evoked by single pulses applied to the spinal cord. When sub-threshold pulses were selectively applied to a cortical motor area, EMG responses from the contralateral leg were facilitated by the delivery of DS to the lumbosacral cord. Finally, based on motor-evoked responses, DS was linked to a greater amplitude of motor output shortly after a calibrated spinal cord contusion. CONCLUSION:Compared to traditional tonic waveforms, DS amplifies both spinal and cortico-spinal input aimed at spinal networks, thus significantly increasing the potential and accelerating the rate of functional recovery after a severe spinal lesion.

Published
2020

2. Two Distinct Stimulus Frequencies Delivered Simultaneously at Low Intensity Generate Robust Locomotor Patterns.

Author

Dose F and Taccola G

Subjects
Action Potentials drug effects, Analysis of Variance, Animals, Animals, Newborn, Biophysics, Excitatory Amino Acid Agonists pharmacology, In Vitro Techniques, Locomotion drug effects, Motor Neurons drug effects, N-Methylaspartate pharmacology, Nerve Net, Patch-Clamp Techniques, Rats, Serotonin pharmacology, Action Potentials physiology, Biophysical Phenomena physiology, Electric Stimulation, Locomotion physiology, Motor Neurons physiology, Spinal Cord cytology
Abstract

Objectives: Explore the primary characteristics of afferent noisy stimuli, which optimally activate locomotor patterns at low intensity., Materials and Methods: Intracellular and extracellular electrophysiological traces were derived from single motoneurons and from ventral roots, respectively. From these recordings, we obtained noisy stimulating protocols, delivered to a dorsal root (DR) of an isolated neonatal rat spinal cord, while recording fictive locomotion (FL) from ventral roots., Results: We decreased complexity of efficient noisy stimulating protocols down to single cell spikes. Then, we identified four main components within the power spectrum of these signals and used them to construct a basic multifrequency protocol of rectangular impulses, able to induce FL. Further disassembling generated the minimum stimulation paradigm that activated FL, which consisted of a pair of 35 and 172 Hz frequency pulse trains, strongly effective at low intensity when delivered either jointly to one lumbosacral DR or as single simultaneous trains to two distinct DRs. This simplified pulse schedule always activated a locomotor rhythm, even when delivered for a very short time (500 ms). One prerequisite for the two-frequency protocol to activate FL at low intensity when applied to sacrocaudal afferents was the ability to induce ascending volleys of greater amplitude., Conclusion: Multifrequency protocols can support future studies in defining the most effective characteristics for electrical stimulation to reactivate stepping following motor injury., (© 2016 International Neuromodulation Society.)

Published
2016
Full Text
View/download PDF

3. Electrical Stimulation Able to Trigger Locomotor Spinal Circuits Also Induces Dorsal Horn Activity.

Author

Dingu N, Deumens R, and Taccola G

Subjects
Animals, Animals, Newborn, Biophysics, Electric Stimulation, In Vitro Techniques, Linear Models, Rats, Rats, Wistar, Spinal Cord physiology, Time Factors, Action Potentials physiology, Nerve Net physiology, Spinal Cord anatomy & histology, Spinal Cord Dorsal Horn physiology, Spinal Nerve Roots physiology
Abstract

Objectives: Investigate whether electrical stimulation of the spinal cord adapted to trigger locomotor patterns additionally influences dorsal horn networks., Materials and Methods: An in vitro model of isolated neonatal rat spinal cord was used to repetitively deliver electrical stimuli to lumbar dorsal roots and record from homolateral lumbar dorsal roots and ventral roots., Results: Repetitive electrical lumbar dorsal root stimulation can affect both locomotor rhythms derived from ventral neuronal circuits and activity from dorsal neuronal circuits., Conclusion: These data suggest that neuro-electrostimulation protocols can simultaneously activate functionally distinct spinal neuronal circuits., (© 2015 International Neuromodulation Society.)

Published
2016
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results