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Polybrominated diphenyl ether (PBDE) contamination is common in aquatic environments and can severely damage aquatic organisms. However, there is a lack of information on the response and self-adaptation mechanisms of these organisms. Chlorella pyrenoidosa was treated with 2,2',4,4'-tetrabromodiphenyl ether (BDE47), causing significant growth inhibition, pigment reduction, oxidative stress, and chloroplast atrophy. Photosynthetic damage contributed to inhibition, as indicated by Fv/Fm, Chl a fluorescence induction, photosynthetic oxygen evolution activity, and photosystem subunit stoichiometry. Here, Chl a fluorescence induction and quinone electron acceptor (Q A - ) reoxidation kinetics showed that the PSII donor and acceptor sides were insensitive to BDE47. Quantitative analyses of D1 and PsaD proteins illustrated that PSII and PSI complexes were the main primary targets of photosynthesis inhibition by BDE47. Significant modulation of PSII complex might have been caused by the potential binding of BDE47 on D1 protein, and molecular docking was performed to investigate this. Increased activation of antioxidant defense systems and photosystem repair as a function of exposure time indicated a positive resistance to BDE47. After a 5-day exposure, 23 % of BDE47 was metabolized. Our findings suggest that C. pyrenoidosa has potential as a bioremediator for wastewater-borne PBDEs and can improve our understanding of ecological risks to microalgae., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)

The persistent organic pollutant perfluorooctanoic acid (PFOA) is ubiquitous in aquatic environments. However, little is known about its toxicity to microalgae or the mechanisms by which they may self-adapt to it. We found that growth of the bloom-forming cyanobacterium Microcystis aeruginosa was initially inhibited, with inhibition attenuated after 12 d of PFOA exposure. Growth inhibition gradually decreased and stabilized over time. With increasing PFOA concentration, reactive oxygen species levels and superoxide dismutase and photosystem II activity significantly increased, while respiration, NDH-1 activity, and total carbohydrate content significantly decreased. Self-adaptation mechanisms included antioxidant pathways, energy transfer and distribution of photosystems, and repair of the PSI and NDH complexes. The patterns of change in these parameters were consistent with those of the expression levels of genes in their associated metabolic pathways. Our data suggest that PSII overcompensation might be a strategy by which M. aeruginosa contends with oxidative stress induced by PFOA. Multiple downstream photosynthesis-related proteins were upregulated as a function of PFOA exposure time. These findings may help elucidate physiological, genetic stress and self-adaptive responses of microalgae to PFOA exposure., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)