23 results on '"Tsukuda, M."'
Search Results
2. A fully digital feedback control of gate driver for current balancing of parallel connected power devices
- Author
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Next Generation Power Electronics Research Centre, Kyushu Inst. of Tech., Kitakyushu 808-0196, Japan, Green Electronics Research Institute Kitakyushu (GRIK), Kitakyushu 808-0135, Japan, Tripathi, R.N., Tsukuda, M., Omura, I., Next Generation Power Electronics Research Centre, Kyushu Inst. of Tech., Kitakyushu 808-0196, Japan, Green Electronics Research Institute Kitakyushu (GRIK), Kitakyushu 808-0135, Japan, Tripathi, R.N., Tsukuda, M., and Omura, I.
- Abstract
type:Journal Article, Parallel connected power devices such as Insulated Gate Bipolar Transistors (IGBTs) can be used to realize a system with higher current and higher power rating. However, the operation of parallel connected IGBTs is prone to unbalancing due to variation in parameters of the semiconductor devices and asymmetric parallel system. In this paper, feedback control is proposed for peak overshoot minimization as well as current balancing of parallel connected IGBTs. A fully digital feedback control (DFC) is implemented using the universal clock for balanced operation of the two parallel connected IGBTs.
- Published
- 2020
3. Bias voltage criteria of gate shielding effect for protecting IGBTs from shoot-through phenomena
- Author
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Advanced Power Devices Laboratory, Green Electronics Research Institute, Kitakyushu, Japan, Next Generation Power Electronics Research Center, Kyushu Institute of Technology, Kitakyushu, Japan, Next Generation Power Electronics Research Center, Kyushu Institute of Technology, Kitakyushu, Japan, Advanced Power Devices Laboratory, Green Electronics Research Institute, Kitakyushu, Japan, Tsukuda, M., Abe, S., Hasegawa, K., Ninomiya, T., Omura, I., Advanced Power Devices Laboratory, Green Electronics Research Institute, Kitakyushu, Japan, Next Generation Power Electronics Research Center, Kyushu Institute of Technology, Kitakyushu, Japan, Next Generation Power Electronics Research Center, Kyushu Institute of Technology, Kitakyushu, Japan, Advanced Power Devices Laboratory, Green Electronics Research Institute, Kitakyushu, Japan, Tsukuda, M., Abe, S., Hasegawa, K., Ninomiya, T., and Omura, I.
- Abstract
type:Journal Article, In this paper, we propose the criteria of bias voltage from parasitic capacitance and demonstrate the criteria in an experiment with the present IGBT. The bias voltage criteria are theoretically predicted for the new generation IGBT based on the scaling principle. For safe switching, the required gate voltage bias is predicted to be −1.2V or less for the present IGBTs and −6V or less is required to completely cancel the gate noise voltage. From the IGBT design, the bias voltage of scaling IGBT requires −2V to completely cancel the gate noise voltage.
- Published
- 2020
4. 16-channnel Micro Magnetic Flux Sensor Array for IGBT Current Distribution Measurement
- Author
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Tomonaga, H., Tsukuda, M., Okoda, S., Noda, R., Tashiro, K., and Omura, I.
- Subjects
Digital calibration ,Hardware_INTEGRATEDCIRCUITS ,Film sensor ,Current distribution ,Flat sensitivity ,Reliability analysis ,Current crowding ,Magnetic flux ,IGB - Abstract
Current crowding of IGBT and power diode in a chip or among chips is a barrier to the realization of highly-reliable power module and power electronics system. Current crowding occurs because of the parasitic inductance, difference of chip characteristics or temperature imbalance among chips. Although current crowding among IGBT or power diode chips has been analysed on numerical simulations, no sensor with sufficiently high special resolution and fast measurement time has yet been demonstrated. Therefore, the author developed and demonstrated 16-channel flat sensitivity sensor array for IGBT current distribution measurement. The sensor array consists of tiny-scale film sensors with analog amps and shield case against noise. The array and digital calibration method will be applied for reliability analysis, designing and screening of IGBT modules., ESREF 2015, 26th European Symposium on Reliability of Electron Devices, Failure Physics and Analysis, Oct 5-9, 2015, Centre de Congrès Pierre Baudis, Toulouse, France
- Published
- 2015
5. Calculation of single event burnout failure rate for high voltage devices under satellite orbit without fitting parameters
- Author
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Sudo, M., Nagamatsu, T., Tsukuda, M., Omura, I., Sudo, M., Nagamatsu, T., Tsukuda, M., and Omura, I.
- Abstract
Increase of power bus voltages in spacecraft are expected with the power demand growth. Accordingly, high voltage semiconductor devices in the power supply system will be required to withstand high energy and high flux cosmic ray environment. In this paper, we propose a new formula to calculate failure rate for power semiconductor devices in space application.
- Published
- 2019
6. Development of fast short-circuit protection system for advanced IGBT
- Author
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Ichiki, M., Arimoto, T., Abe, S., Tsukuda, M., Omura, I., Ichiki, M., Arimoto, T., Abe, S., Tsukuda, M., and Omura, I.
- Abstract
The higher current density of IGBTs has made it difficult to achieve high short-circuit withstand capability as well as good conduction characteristics. Therefore, a fast and reliable protection system is required for the safe operation of IGBT. This paper determines the short-circuit safe protection area (SCSPA) for advanced IGBT that has no short-circuit withstand capability and proposes the protection system satisfied this SCSPA. The fast protection is brought by PCB Rogowski coil and digital gate driver using digital circuit (FPGA). Experimental results verify that short-circuit detection time is 70 ns, shut-down time is reduced by controlling the gate resistance at turn-off.
- Published
- 2019
7. Clamp type built-in current sensor using PCB in high-voltage power modules
- Author
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Tsukuda, M., Nakashima, K., Tabata, S., Hasegawa, K., Omura, I., Tsukuda, M., Nakashima, K., Tabata, S., Hasegawa, K., and Omura, I.
- Abstract
type:Journal Article, High reliability is required for power modules because they are increasingly demanded as key devices in an energy-saving society. However, commercially available current sensors like current transformers and Rogowski coils are still of large size. Therefore, it is impossible to measure the current in small parts. In addition, long wiring with large sensors reduces the performance of power modules. In previous studies, we proposed a current sensor of PCB Rogowski coil with a fishbone pattern. The sensor is sufficiently small and thin with high accuracy for power module application. The PCB current sensor can be used as a built-in current sensor in power modules from the viewpoint of size. However, the sensor requires further improvement to be attached to bonding wire from a usability perspective. We propose a new pattern for the clamp-type PCB sensor and fabricate the PCB sensor as a built-in sensor in a power module. With the new pattern, it is possible to move and widen the gap for clamping. The signal error is small even though the sensor is inclinedly attached to the bonding wire. The accuracy is confirmed by a comparison between the clamp-type PCB sensor in the module and a commercially available current sensor outside the module. The clamp-type PCB sensor can be applied for intelligent power modules as well as current measurement for power electronic converters., source:https://doi.org/10.1016/j.microrel.2017.06.026
- Published
- 2019
8. Modelling of the shoot-through phenomenon introduced by the next generation IGBT in inverter applications
- Author
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Department of Life Science and System Engineering, Kyushu Institute of Technology, Green Electronics Research Institute, Department of Electrical and Electronic Engineering, Tokyo Metropolitan University, Abe, S., Hasegawa, K., Tsukuda, M., Wada, K., Omura, I., Ninomiya, T., Department of Life Science and System Engineering, Kyushu Institute of Technology, Green Electronics Research Institute, Department of Electrical and Electronic Engineering, Tokyo Metropolitan University, Abe, S., Hasegawa, K., Tsukuda, M., Wada, K., Omura, I., and Ninomiya, T.
- Abstract
type:Journal Article, The shoot-through phenomenon has not been fully discussed for high-power inverters with IGBTs. This is because a negative gate voltage is applied to IGBTs during off states. Recently, attention is paid to an improved gate driver with only a positive gate voltage in order to meet demands for simplification, integration, and reduction in power consumption as well as in cost of the gate driver. Moreover, the threshold voltage of the next-generation IGBT will decrease with microfabrication techniques of the gate structure. This will make the shoot-through phenomenon severer and degrade the inverter reliability with the next-generation IGBTs. The influence of the parasitic parameters in both the IGBT and circuit on the shoot-through mechanism has not been investigated so far.This paper clarifies the shoot-through mechanism and investigates the impact of the next generation IGBTs on the inverter reliability. The influence of the internal capacitance of IGBT including stray inductance on inverter reliability is experimentally confirmed., source:https://doi.org/10.1016/j.microrel.2017.06.087
- Published
- 2019
9. Micro PCB Rogowski coil for current monitoring and protection of high voltage power modules
- Author
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Advanced Power Device Laboratory, Green Electronics Research Institute, Next Generation Power Electronics Research Center, Kyushu Institute of Technology, Next Generation Power Electronics Research Center, Kyushu Institute of Technology, Tsukuda, M., Koga, M., Nakashima, K., Omura, I., Advanced Power Device Laboratory, Green Electronics Research Institute, Next Generation Power Electronics Research Center, Kyushu Institute of Technology, Next Generation Power Electronics Research Center, Kyushu Institute of Technology, Tsukuda, M., Koga, M., Nakashima, K., and Omura, I.
- Abstract
type:Journal Article, We have developed a printed circuit board Rogowski coil for monitoring of current and protection of high-voltage power modules and packages. It is small, thin, and inexpensive current sensor and is almost the ideal Rogowski coil because of its fishbone pattern. For noise reduction under high-voltage/-current conditions in a module, shield layers and coaxial connector are employed. In addition, a new, fast simulation tool was developed to optimize the main coil pattern for realization of arbitrary printed circuit board geometry in specific, limited spaces.
- Published
- 2018
10. Structure Oriented Compact Model for Advanced Trench IGBTs without Fitting Parameters for Extreme Condition: Part II
- Author
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Kyushu Institute of Technology, The International Centre for the Study of East Asian Development, Takaishi, J., Harada, S., Tsukuda, M., Omura, I., Kyushu Institute of Technology, The International Centre for the Study of East Asian Development, Takaishi, J., Harada, S., Tsukuda, M., and Omura, I.
- Abstract
type:Journal Article, Compact model for expressing turn-off waveform for advanced trench gate IGBTs is proposed even under high current density condition. The model is analytically formulated only with device structure parameters so that no fitting parameters are required. The validity of the model is confirmed with TCAD simulation for 1.2 kV to 6.5 kV class IGBTs. The proposed turn-off model is sufficiently accurate to calculate trade-off curve between turn-off loss and saturation collector voltage under extremely high current conduction, so that the model can be used for system design with the advanced trench gate IGBTs., ESREF 2014, 25th EUROPEAN SYMPOSIUM ON RELIABILITY OF ELECTRON DEVICES,FAILURE PHYSICS AND ANALYSIS, Sep 29–Oct 3, 2014, Technische Universität Berlin
- Published
- 2017
11. Real time degradation monitoring system for high power IGBT module under power cycling test
- Author
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Department of Electrical Engineering/Electronics, Kyushu Institute of Technology, Department of Electrical Engineering/Electronics, Kyushu Institute of Technology, The International Centre for the Study of East Asian Development, Watanabe, A., Tsukuda, M., Omura, I., Department of Electrical Engineering/Electronics, Kyushu Institute of Technology, Department of Electrical Engineering/Electronics, Kyushu Institute of Technology, The International Centre for the Study of East Asian Development, Watanabe, A., Tsukuda, M., and Omura, I.
- Abstract
type:Journal Article, A “real time” monitoring system which enables to observe internal degradation process to failure of power semiconductors under power cycling test is proposed. The system was realized by combining a scanning acoustic tomography (SAT/SAM), power stress controlling, device cooling, water jet system and chip temperature monitoring. Two contradictory problems, namely, electrically wiring for power cycling and waterproof of device for SAT imaging were compatible with each other by experimental setup with an original water tank. Self-heating of power devices was supressed by controlling temperature of water which is couplant of ultrasonic wave for the SAT. A demonstration of this system was performed by using an IGBT module which maximum rating of collector current was 400 A (DC)., 24th European Symposium on Reliability of Electron Devices, Failure Physics and Analysis. Schedule, September 30-October 4, 2013, Venue, Arcachon, France
- Published
- 2017
12. Failure Analysis of Power Devices Based on Real-Time Monitoring
- Author
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Department of Electrical Engineering/Electronics, Kyushu Institute of Technology/Next generation power electronics research center, Kyushu Institute of Technology, Electronics Research Group for Sustainability, Asian Growth Research Institute/Next generation power electronics research center, Kyushu Institute of Technology, Watanabe, A., Tsukuda, M., Omura, I., Department of Electrical Engineering/Electronics, Kyushu Institute of Technology/Next generation power electronics research center, Kyushu Institute of Technology, Electronics Research Group for Sustainability, Asian Growth Research Institute/Next generation power electronics research center, Kyushu Institute of Technology, Watanabe, A., Tsukuda, M., and Omura, I.
- Abstract
type:Journal Article, The aim is to provide failure analysis of power devices based on real-time monitoring. The real-time monitoring provides a time-domain data related to a failure mechanism. The data includes important information about primary failure, which is often lost by conventional post-defect failure analysis. Our system monitors interfaces of component material inside the device by scanning acoustic tomography (SAT) under a power cycling test in addition to electrical and thermal condition of the device. A precursor of the failure in an early stage was indicated by the interface image much earlier than a thermal and an electrical technique. Feature identification and extraction from a series of image data by image processing efficiently pointed out the damaged site before the failure was occurred., ESREF 2015, 26th European Symposium on Reliability of Electron Devices, Failure Physics and Analysis, Oct 5-9, 2015, Centre de Congrès Pierre Baudis, Toulouse, France
- Published
- 2017
13. High-throughput and Full Automatic DBC-Module Screening Tester for High Power IGBT
- Author
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Electronics Research Group for Sustainability, Asian Growth Research Institute, Next Generation Power Electronics Research Center, Kyushu Institute of Technology, Next Generation Power Electronics Research Center, Kyushu Institute of Technology, COPER ELECTRONICS CO., LTD., C.D.N. CORPORATION, HOH KOH SYA Co. Ltd., Tsukuda, M., Tomonaga, H., Okoda, S., Noda, R., Tashiro, K., Omura, I., Electronics Research Group for Sustainability, Asian Growth Research Institute, Next Generation Power Electronics Research Center, Kyushu Institute of Technology, Next Generation Power Electronics Research Center, Kyushu Institute of Technology, COPER ELECTRONICS CO., LTD., C.D.N. CORPORATION, HOH KOH SYA Co. Ltd., Tsukuda, M., Tomonaga, H., Okoda, S., Noda, R., Tashiro, K., and Omura, I.
- Abstract
type:Journal Article, We developed a high-throughput screening tester for DBC-module of IGBT. The tester realizes a new screening test with current distribution in addition to a conventional switching test. It consists of a power circuit, a replaceable test head, sensor array module and digitizer with LabVIEW program. Therefore, all kinds of DBC-modules can be screened by switching the test head. The tester acquires magnetic field signals and displays GO/NOGO judgment automatically after digital calibration and signal processing in 10 seconds. It is expected to be applied for screening in a production line and analysis in order to prevent the failure of power modules., ESREF 2015, 26th European Symposium on Reliability of Electron Devices, Failure Physics and Analysis, Oct 5-9, 2015, Centre de Congrès Pierre Baudis, Toulouse, France
- Published
- 2017
14. Short-Circuit Protection for an IGBT with Detecting the Gate Voltage and Gate Charge
- Author
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Department of Biological Functions and Engineering, Kyushu Institute of Technology, Department of Electrical Engineering and Electronics, Kyushu Institute of Technology, The International Centre for the Study of East Asian Development (ICSEAD), Hasegawa, K., Yamamoto, K., Yoshida, H., Hamada, K., Tsukuda, M., Omura, I., Department of Biological Functions and Engineering, Kyushu Institute of Technology, Department of Electrical Engineering and Electronics, Kyushu Institute of Technology, The International Centre for the Study of East Asian Development (ICSEAD), Hasegawa, K., Yamamoto, K., Yoshida, H., Hamada, K., Tsukuda, M., and Omura, I.
- Abstract
type:Journal Article, This paper proposes a new short-circuit protection method for an IGBT. The proposed method is characterized by detecting not only gate charge but also gate voltage of the IGBT. This results in a shorter protection time, compared to the previous method that detects only the gate charge. A real-time monitoring system using an FPGA, A/D converters, and a D/A converter is used for the proposed protection method. Experimental results verify that the proposed method achieves a protection time of 390 ns, which is reduced by 68% compared to the previous method., ESREF 2014, 25th EUROPEAN SYMPOSIUM ON RELIABILITY OF ELECTRON DEVICES,FAILURE PHYSICS AND ANALYSIS, Sep 29–Oct 3, 2014, Technische Universität Berlin
- Published
- 2017
15. 16-channnel Micro Magnetic Flux Sensor Array for IGBT Current Distribution Measurement
- Author
-
Kyushu Institute of Technology, Kyushu Institute of Technology, Asian Growth Research Institute, Kitakyushu, Japan, COPER ELECTRONICS CO., C.D.N. CORPORATION, HOH KOH SYA Co., Ltd., Tomonaga, H., Tsukuda, M., Okoda, S., Noda, R., Tashiro, K., Omura, I., Kyushu Institute of Technology, Kyushu Institute of Technology, Asian Growth Research Institute, Kitakyushu, Japan, COPER ELECTRONICS CO., C.D.N. CORPORATION, HOH KOH SYA Co., Ltd., Tomonaga, H., Tsukuda, M., Okoda, S., Noda, R., Tashiro, K., and Omura, I.
- Abstract
type:Journal Article, Current crowding of IGBT and power diode in a chip or among chips is a barrier to the realization of highly-reliable power module and power electronics system. Current crowding occurs because of the parasitic inductance, difference of chip characteristics or temperature imbalance among chips. Although current crowding among IGBT or power diode chips has been analysed on numerical simulations, no sensor with sufficiently high special resolution and fast measurement time has yet been demonstrated. Therefore, the author developed and demonstrated 16-channel flat sensitivity sensor array for IGBT current distribution measurement. The sensor array consists of tiny-scale film sensors with analog amps and shield case against noise. The array and digital calibration method will be applied for reliability analysis, designing and screening of IGBT modules., ESREF 2015, 26th European Symposium on Reliability of Electron Devices, Failure Physics and Analysis, Oct 5-9, 2015, Centre de Congrès Pierre Baudis, Toulouse, France
- Published
- 2017
16. Eosinophilic chronic rhinosinusitis in Japan.
- Author
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Ishitoya J, Sakuma Y, and Tsukuda M
- Subjects
- Chronic Disease, Diagnosis, Differential, Humans, Japan, Nasal Polyps diagnostic imaging, Nasal Polyps pathology, Radiography, Rhinitis physiopathology, Rhinitis therapy, Sinusitis physiopathology, Sinusitis therapy, Eosinophils immunology, Nasal Polyps immunology, Rhinitis diagnosis, Sinusitis diagnosis
- Abstract
Chronic rhinosinusitis is a heterogeneous disease. In Europe and the United States, it has recently been divided into two subgroups: chronic rhinosinusitis with nasal polyps (CRSwNP) and chronic rhinosinusitis without nasal polyps (CRSsNP). The majority of CRSwNP cases have a strong tendency to recur after surgery and show eosinophil-dominant inflammation. However, this definition has proved difficult to apply in Japan and East Asia, because more than half of the CRSwNP cases do not exhibit eosinophil-dominant inflammation in these areas of the world. In Japan in the 1990s, refractory CRSwNP to the standard treatment was focused on in clinical studies and the term "eosinophilic chronic rhinosinusitis" (ECRS) was introduced to identify this subgroup of chronic rhinosinusitis in 2001. ECRS is different from non-ECRS in terms of many clinical features: symptom appearance, occurrence site of nasal polyps, CT scan findings, the histology of nasal polyps, blood examination findings, clinical course after surgery, and co-morbid asthma, etc. In this review, we describe these clinical features and mention how to make a clinical diagnosis of ECRS as well as how to treat it. Finally, we discuss the pathophysiology of ECRS. The concept of ECRS in Japan would be applicable for CRSwNP in other countries including Europe and the United States.
- Published
- 2010
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17. Design, synthesis, and biophysical properties of a helical Abeta1-42 analog: Inhibition of fibrillogenesis and cytotoxicity.
- Author
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Matsuzaki K, Okada T, Tsukuda M, Ikeda K, Sohma Y, Chiyomori Y, Taniguchi A, Nakamura S, Ito N, Hayashi Y, and Kiso Y
- Subjects
- Amino Acid Sequence, Amyloid metabolism, Amyloid toxicity, Amyloid beta-Peptides chemical synthesis, Animals, Biophysical Phenomena, Biophysics, Cross-Linking Reagents chemistry, Membrane Microdomains chemistry, Molecular Sequence Data, Neurons chemistry, Neurons drug effects, Neurons metabolism, PC12 Cells, Peptide Fragments chemical synthesis, Protein Structure, Secondary, Rats, Amyloid antagonists & inhibitors, Amyloid beta-Peptides chemistry, Amyloid beta-Peptides pharmacology, Maleimides chemistry, Peptide Fragments chemistry, Peptide Fragments pharmacology
- Abstract
The aggregation of amyloid beta-peptide (Abeta) into beta-sheet-rich aggregates is a crucial step in the etiology of Alzheimer's disease. Helical forms of Abeta have been suggested to be intermediates in the aggregation process of the peptide in aqueous phase, micelles and membranes. A stable helical Abeta analog would be useful to investigate the role of helical intermediates in fibrillization by Abeta. Here we designed a helical analog by simply cross-linking the Cys residues of A30C, G37C-Abeta1-42 with 1,6-bismaleimidohexane. The analog assumed a weak alpha-helical conformation in model membranes mimicking lipid raft microdomains of neuronal membranes under conditions in which the wild-type Abeta1-42 formed a beta-sheet, indicating the cross-linking locally induced a helical conformation. Furthermore, addition of equimolar helical Abeta analog significantly reduced the amyloid formation and cytotoxicity by Abeta1-42. Thus, our helical Abeta1-42 is not only a model peptide to investigate the role of helical intermediates in fibrillization by Abeta, but also an inhibitor of Abeta-induced cytotoxicity.
- Published
- 2008
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18. Communication strategies and interpersonal skills of instructors of esophageal speech: an observation study.
- Author
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Toth G, Hirose H, and Tsukuda M
- Subjects
- Aged, Attitude of Health Personnel ethnology, Attitude to Health ethnology, Authoritarianism, Catharsis, Chi-Square Distribution, Directive Counseling, Female, Helping Behavior, Humans, Japan, Laryngectomy education, Laryngectomy psychology, Laryngectomy rehabilitation, Male, Middle Aged, Nonverbal Communication psychology, Observation, Self-Help Groups, Social Support, Verbal Behavior, Clinical Competence standards, Communication, Interpersonal Relations, Patient Education as Topic standards, Peer Group, Speech, Esophageal psychology
- Abstract
Objective: In this article, the way that the Instructor of Esophageal Speech (IES) works is considered from an interactive-analytic perspective., Methods: The observation project data were gathered during 6 months in 2004 at the training sessions of the Ginreikai-Asian Federation of Laryngectomees' Association using the Six Category Intervention Analysis framework (6CIA) by a trained communication specialist as an active-observer., Results: Mean scores of each of the six categories and a series of percentage distributions were extracted from the usage-frequency data and interpreted together with the results of Chi-square analysis of usage-frequency units. We found the IES used more frequently authoritative categories and used less facilitative categories. Our results of the data analysis show that the 30 IES used more authoritative interventions and used more frequently the prescription and information giving than the confrontation or cathartic interventions., Conclusion: The results of the present study show that the 6CIA framework has its potential and value as an analytic tool to explore the IES' actual behavior in a specific therapy related context., Practice Implications: We argue for the utility of the 6CIA as an analytic framework to investigate the interpersonal behavior of the IES in the Japanese cultural setting.
- Published
- 2006
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19. Markers of malignant transformation of sinonasal inverted papilloma.
- Author
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Katori H, Nozawa A, and Tsukuda M
- Subjects
- Antibodies, Monoclonal, Carcinoma pathology, Carcinoma, Squamous Cell pathology, ErbB Receptors analysis, Female, Humans, In Situ Hybridization, Ki-67 Antigen analysis, Male, Middle Aged, Nasal Mucosa pathology, Neoplasm Invasiveness, Papilloma pathology, Papillomaviridae classification, Papillomaviridae isolation & purification, Precancerous Conditions pathology, Transforming Growth Factor alpha analysis, Biomarkers, Tumor analysis, Cell Transformation, Neoplastic pathology, Nose Neoplasms pathology, Papilloma, Inverted pathology, Paranasal Sinus Neoplasms pathology
- Abstract
Aim: To measure HPV status, epidermal growth factor receptor (EGFR) and transforming growth factor-alpha (TGF-alpha) expression and Ki-67 index in exophytic papilloma (EP), inverted papilloma (IP) with dysplasia, IP with carcinoma and invasive squamous cell carcinoma (SCC)., Methods: Forty-four patients with sinonasal papilloma and invasive SCC were selected. The nasal tissues were stained with monoclonal antibodies to EGFR, TGF-alpha and Ki-67. The results were analysed using quantitative immunohistochemical analysis. In situ hybridization studies for HPV DNA for 6/11, 16/18 and 31/33 were also performed on the tissue., Results: Significant increase of EGFR and TGF-alpha was observed in IP with severe dysplasia, IP with carcinoma and invasive SCC compared to IP with mild dysplasia and control nasal mucosa. And a serial upreguration in terms of Ki-67 index in IP with dysplasia was observed. Among IP, HPV 6/11-positive was present in 42% tumour and HPV 16/18-positive was present in 31% of tumours. Among HPV 6/11 and 16/18-positive IP, significant increase of EGFR and Ki-67 index were observed., Conclusion: Pre-cancerous lesions of IP exhibited elevated levels of EGFR and TGF-alpha and these expression may be associated with early events in IP carcinogenesis. HPV infection may be an early event in a multistep process of malignant formation of IP.
- Published
- 2005
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20. Induction of SPARC by VEGF in human vascular endothelial cells.
- Author
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Kato Y, Lewalle JM, Baba Y, Tsukuda M, Sakai N, Baba M, Kobayashi K, Koshika S, Nagashima Y, Frankenne F, Noël A, Foidart JM, and Hata RI
- Subjects
- Cells, Cultured, Humans, Osteonectin genetics, RNA, Messenger biosynthesis, Vascular Endothelial Growth Factor A, Vascular Endothelial Growth Factors, Endothelial Growth Factors physiology, Endothelium, Vascular metabolism, Lymphokines physiology, Osteonectin biosynthesis
- Abstract
SPARC/osteonectin/BM-40 is a matricellular protein that is thought to be involved in angiogenesis and endothelial barrier function. Previously, we have detected high levels of SPARC expression in endothelial cells (ECs) adjacent to carcinomas of kidney and tongue. Although SPARC-derived peptide showed an angiogenic effect, intact SPARC itself inhibited the mitogenic activity of vascular endothelial growth factor (VEGF) for ECs by the inhibiting phosphorylation of flt-1 (VEGF receptor 1) and subsequent ERK activation. Thus, the role of SPARC in tumor angiogenesis, stimulation or inhibition, is still unclear. To clarify the role of SPARC in tumor growth and progression, we determined the effect of VEGF on the expression of SPARC in human microvascular EC line, HMEC-1, and human umbilical vein ECs. VEGF increased the levels of SPARC protein and steady-state levels of SPARC mRNA in serum-starved HMEC-1 cells. Inhibitors (SB202190 and SB203580) of p38, a mitogen-activated protein (MAP) kinase, attenuated VEGF-stimulated SPARC production in ECs. Since intact SPARC inhibits phosphorylation ERK MAP kinase in VEGF signaling, it was suggested that SPARC plays a dual role in the VEGF functions, tumor angiogenesis, and extravasation of tumors mediated by the increased permeability of endothelial barrier function., (Copyright 2001 Academic Press.)
- Published
- 2001
- Full Text
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21. Reduced expression of p16 and p27 proteins in nasopharyngeal carcinoma.
- Author
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Baba Y, Tsukuda M, Mochimatsu I, Furukawa S, Kagata H, Satake K, Koshika S, Nakatani Y, Hara M, Kato Y, and Nagashima Y
- Subjects
- Carcinoma, Squamous Cell pathology, Cyclin D1 metabolism, Cyclin-Dependent Kinase Inhibitor p27, Cyclin-Dependent Kinases antagonists & inhibitors, Enzyme Inhibitors metabolism, Genes, Tumor Suppressor physiology, Humans, Immunoenzyme Techniques, Nasopharyngeal Neoplasms pathology, Nasopharynx metabolism, Carcinoma, Squamous Cell metabolism, Cell Cycle Proteins metabolism, Cyclin-Dependent Kinase Inhibitor p16 metabolism, Nasopharyngeal Neoplasms metabolism, Tumor Suppressor Proteins metabolism
- Abstract
Nasopharyngeal carcinoma (NPC) is a malignant tumor with a high incidence in east Asian countries. Inactivation of cyclin-dependent kinase (CDK) inhibitors (CKIs) and overexpression of G1 cyclin has been thought to be important for tumor development. To determine whether reduction of CKI (p16 and p27) expression was associated with NPC development, we performed immunohistochemical staining of NPC specimens from 20 patients. We found that p16 and p27 proteins were negative in 8 of 20 and 16 of 20 cases, respectively; that either p16 or p27 proteins were negative in 17 of 20; and that both p16 and p27 were negative in 7 of 20. Excepting the cases in which both CKIs were negative, negativity of p27 alone was statistically higher than that of p16 (9/20 versus 1/20, P = .022), suggesting that the reduction of p27 protein is an important event for the multi-step process of NPC development.
- Published
- 2001
22. Properties of protein kinase C subspecies in human platelets.
- Author
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Tsukuda M, Asaoka Y, Sekiguchi K, Kikkawa U, and Nishizuka Y
- Subjects
- Amino Acid Sequence, Arachidonic Acid, Arachidonic Acids pharmacology, Blood Proteins analysis, Chromatography, Gel, Diglycerides pharmacology, Humans, Molecular Sequence Data, Phosphatidylserines pharmacology, Phosphorylation, Blood Platelets enzymology, Isoenzymes blood, Phosphoproteins, Protein Kinase C blood
- Abstract
Protein kinase C (PKC) from human platelets was resolved into two fractions by hydroxyapatite column chromatography. One of the enzymes was indistinguishable from the brain type III PKC having alpha-sequence in its kinetic and immunological properties. The other enzyme was kinetically different from any of the brain PKC subspecies so far isolated, although it resembled the brain type II PKC having beta-sequence. With H1 histone as substrate, this platelet enzyme was not very sensitive to Ca2+, and activated partly by phosphatidylserine plus diacylglycerol or by free arachidonic acid. Both platelet enzymes could phosphorylate the P47 protein in vitro, but the enzyme physiologically responsible for the P47 protein phosphorylation in the activated platelets remains to be identified.
- Published
- 1988
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23. Three distinct forms of rat brain protein kinase C: differential response to unsaturated fatty acids.
- Author
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Sekiguchi K, Tsukuda M, Ogita K, Kikkawa U, and Nishizuka Y
- Subjects
- Animals, Calcium pharmacology, Chromatography, High Pressure Liquid, Diglycerides pharmacology, Isomerism, Phosphatidylserines pharmacology, Rats, Brain enzymology, Fatty Acids, Unsaturated pharmacology, Protein Kinase C metabolism
- Abstract
Although the three distinct forms of protein kinase C isolated from rat brain soluble fraction are structurally very similar, they respond differently to free unsaturated fatty acids such as arachidonic acid to exhibit their catalytic activity. Type I enzyme encoded by gamma-sequence, as predicted by cDNA clone analysis, responds to these fatty acids only slightly, whereas Type III enzyme determined by alpha-sequence is activated by free unsaturated fatty acids in the presence of Ca2+ in a comparable manner to phosphatidylserine plus diacylglycerol. Type II, a mixture of two enzymes encoded by beta I- and beta II-sequence, resulting from alternative splicing, shows properties in between those of Type I and Type III. Some of these forms of protein kinase C may function at a relatively later phase of cellular responses when large amounts of unsaturated fatty acids and Ca2+ are mobilized.
- Published
- 1987
- Full Text
- View/download PDF
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