1. Application of high-speed counter-current chromatography for preparative separation of cyclic peptides from Vaccaria segetalis.
- Author
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Wang X, Dong H, Liu Y, Yang B, Wang X, and Huang L
- Subjects
- Acetates chemistry, Alkanes chemistry, Chromatography, High Pressure Liquid, Methanol chemistry, Nuclear Magnetic Resonance, Biomolecular, Seeds chemistry, Spectrometry, Mass, Electrospray Ionization, Countercurrent Distribution methods, Drugs, Chinese Herbal chemistry, Peptides, Cyclic isolation & purification, Vaccaria chemistry
- Abstract
Following an initial clean-up step on silica gel, high-speed counter-current chromatography (HSCCC) was used to separate cyclic peptides from an extract of the seeds of Vaccaria segetalis. The two-phase solvent system used for HSCCC separation was composed of petroleum ether-ethyl acetate-methanol-water at an optimized volume ratio of 0.5:3.5:1:5. From 190 mg of crude extract, 38.0 mg of segetalin B and 28.5 mg of segetalin A were obtained with purities of 98.1% and 95.6% as determined by HPLC, respectively. The chemical structures of the target compounds were confirmed by high resolution electrospray ionization time of flight MS (HRESI-TOF-MS) and (1)H NMR analyses., (Copyright © 2011. Published by Elsevier B.V.)
- Published
- 2011
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